莱氏野村菌Nr5772菌株对甜菜夜蛾的毒力研究
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摘要
旨在确定莱氏野村菌Nr 5772菌株对甜菜夜蛾的毒力,为甜菜夜蛾的生物防治提供新的技术手段。以甜菜夜蛾幼虫为实验材料,采用浸渍法,测定莱氏野村菌Nr5772菌株分生孢子对甜菜夜蛾3、4、5龄幼虫的LC50和LT50。结果表明莱氏野村菌5×109个孢子/mL对甜菜夜蛾3、4、5龄幼虫的LT50分别为4.44 d、4.48 d和4.85 d,LC50分别为2.71×106个/mL、5.61×106个/mL和1.75×107个孢子/mL。莱氏野村菌Nr5772菌株对甜菜夜蛾幼虫有较强的致病力,孢子浓度越高、幼虫龄期越低则致病力越强。
The aim is to investigate the virulence of Nomuraea rileyi Nr5772 isolate against beet armyworm Spodoptera exigua, and provide a new technique for biological control of the pest. The larvae of beet armyworm were used as materials. Dipping method was adopted to test the LC50 and LT50 of the 3 rd, 4 th and 5 th instar beet armyworm larvae in the conidia suspension of N. rileyi Nr5772 isolate. The results showed that LT50 of the 3 rd,4 th and 5 th instar larvae was 4.44 d, 4.48 d and 4.85 d, respectively, when exposed to 5×109 conidia/mL N.rileyi suspension; and the LC50 was 2.71×106 conidia/mL, 5.61×respectively. In conclusion, N. rileyi Nr5772 isolate has effective pathogenicity to beet armyworm larvae and the virulence becomes stronger along with the increase of conidia concentration and the decrease of the larva instar.
The aim is to investigate the virulence of Nomuraea rileyi Nr5772 isolate against beet armyworm Spodoptera exigua, and provide a new technique for biological control of the pest. The larvae of beet armyworm were used as materials. Dipping method was adopted to test the LC50 and LT50 of the 3 rd, 4 th and 5 th instar beet armyworm larvae in the conidia suspension of N. rileyi Nr5772 isolate. The results showed that LT50 of the 3 rd,4 th and 5 th instar larvae was 4.44 d, 4.48 d and 4.85 d, respectively, when exposed to 5×109 conidia/mL N.rileyi suspension; and the LC50 was 2.71×106 conidia/mL, 5.61×respectively. In conclusion, N. rileyi Nr5772 isolate has effective pathogenicity to beet armyworm larvae and the virulence becomes stronger along with the increase of conidia concentration and the decrease of the larva instar.
引文
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[10]彭好文,李启秦,林纬.生物防治研究及其概况[J].广西农业生物科学, 2004(6):1008-3464.
[11]杨新军,林华峰,李茂业,等.莱氏野村菌Nr05菌株的培养性状及其对斜纹夜蛾的毒力[J].中国生物防治,2007,23(1):44-48.
[12]周晓梅,黄炳球.斜纹夜蛾抗药性及防治对策的研究进展[J].昆虫知识,2002,9(2):98-102.
[13]唐维媛.不同地域莱氏野村菌的培养性状及对斜纹夜蛾的毒力[J].湖南农业大学学报:自科版,2013,39(3):279-281.
[14]孙庚,刘少武,常秀辉,等.一种改进的斜纹夜蛾人工饲养技术的效果研究[J].山东农业科学, 2015,47(2):04-106.
[15]刘国全. DPS数据处理系统在植物保护专业中的应用[J].中国植保导刊, 2013,02(2):1672-1680.
[16]周世力.昆虫的体壁与真菌的入侵[J].江汉大学学报:人文科学版, 1997,16(6):11-13.
[17] Boucias D, Liu S, Meagher R, et al. Fungal dimorphism in the entomopathogenic fungus Metarhizium rileyi:Detection of an in vivo quorum-sensing system[J]. Journal of Invertebrate Pathology,2016,136:100-108.
[18]裘晖,吴振强,梁世中.金龟子绿僵菌及其杀虫机理[J].农药,2004,8(43):342-345.
[19] Dubovskiy I M, Whitten M M A, Yaroslavtseva O N, et al. Can insects develop resistance to insect pathogenic fungi[J]. PLoS One,2013,8(4):e60248.
[20]何雪香. David Merritt, David Holdom.莱氏野村菌对棉铃虫幼虫的致病机理[A].全国生物防治暨第八届杀虫微生物学术研讨会论文摘要集[C].2000:3-4.
[21] Enríquez-vara J N, Córdoba-aguilar A, Guzmán-Franco A W, et al.Is survival after pathogen exposure explained by host’s immune strength? A test with two species of white grubs(Coleoptera:Scarabaeidae)exposed to fungal infection[J]. Environmental Entomology, 2012,41(4):959-965.
[2]刘琴,徐健,殷向东,等.莱氏野村菌的生物学特性及其对甜菜夜蛾的致病力研究[J].江苏农业学报,2009,25(1):68-72.
[3]李峰,谢明,万方浩,等.莱氏野村菌研究及应用进展[A]//第二届全国绿色环保农药新技术、新产品交流会论文集[C].厦门:中国植物保护协会,2003:72-79
[4]袁盛勇,孔琼,张宏瑞,等.莱氏野村菌对斜纹夜蛾幼虫的致病性[J].植物保护,2010,36(5):171-173
[5]蒋靖靖,马忠友,李佳丽,等.重要虫生真菌莱氏野村菌芽生孢子的形态发生[J].菌物学报,2010,29(3):340-346.
[6] Perinotto W M S, Terra A L M, Angelo I C, et al. Nomuraea rileyi as biological control agents of Rhipicephalus microplus tick[J].Parasitlolgy Research,2012,111(4):1743-1748
[7]方文杰,龚进兴,李金景.厦门地区甜菜夜蛾的发生与防治[J].华东昆虫学报,1998,7(2):73-79
[8]陈亮,李兵,浦冠勤.斜纹夜蛾抗药性产生的原因及防治对策[J].中国蚕业,2009,30(2):16-19.
[9]罗礼智,曹雅忠,江幸福.甜菜夜蛾发生危害特点及其趋势分析[J].植物保护, 2000,26(3):37-39.
[10]彭好文,李启秦,林纬.生物防治研究及其概况[J].广西农业生物科学, 2004(6):1008-3464.
[11]杨新军,林华峰,李茂业,等.莱氏野村菌Nr05菌株的培养性状及其对斜纹夜蛾的毒力[J].中国生物防治,2007,23(1):44-48.
[12]周晓梅,黄炳球.斜纹夜蛾抗药性及防治对策的研究进展[J].昆虫知识,2002,9(2):98-102.
[13]唐维媛.不同地域莱氏野村菌的培养性状及对斜纹夜蛾的毒力[J].湖南农业大学学报:自科版,2013,39(3):279-281.
[14]孙庚,刘少武,常秀辉,等.一种改进的斜纹夜蛾人工饲养技术的效果研究[J].山东农业科学, 2015,47(2):04-106.
[15]刘国全. DPS数据处理系统在植物保护专业中的应用[J].中国植保导刊, 2013,02(2):1672-1680.
[16]周世力.昆虫的体壁与真菌的入侵[J].江汉大学学报:人文科学版, 1997,16(6):11-13.
[17] Boucias D, Liu S, Meagher R, et al. Fungal dimorphism in the entomopathogenic fungus Metarhizium rileyi:Detection of an in vivo quorum-sensing system[J]. Journal of Invertebrate Pathology,2016,136:100-108.
[18]裘晖,吴振强,梁世中.金龟子绿僵菌及其杀虫机理[J].农药,2004,8(43):342-345.
[19] Dubovskiy I M, Whitten M M A, Yaroslavtseva O N, et al. Can insects develop resistance to insect pathogenic fungi[J]. PLoS One,2013,8(4):e60248.
[20]何雪香. David Merritt, David Holdom.莱氏野村菌对棉铃虫幼虫的致病机理[A].全国生物防治暨第八届杀虫微生物学术研讨会论文摘要集[C].2000:3-4.
[21] Enríquez-vara J N, Córdoba-aguilar A, Guzmán-Franco A W, et al.Is survival after pathogen exposure explained by host’s immune strength? A test with two species of white grubs(Coleoptera:Scarabaeidae)exposed to fungal infection[J]. Environmental Entomology, 2012,41(4):959-965.