细胞传代致弱的禽偏肺病毒JC毒株致病性变化的研究
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摘要
将禽偏肺病毒(aMPV)JC株在Vero细胞上传代传至第50代时,细胞适应毒株毒价为105.6TCID50/m L;雏鸡致病性试验结果显示,aMPV JC株细胞传代第50代与亲本毒F0相比感染雏鸡发病率降低,病理变化减轻,表明第F50细胞适应毒的毒力减弱。
Avian metapneumovirus( a MPV) JC strain was propagated in Vero cells. The virus titer reached to 105. 6 TCID5 0/m L after 50 passages. Compared to parental virus,the morbidity rate decreased and the pathological changes were alleviated in chicken after being challenged with passage 50 virus. It indicated that the virulence of F50 cell adapted strain was attenuated.
Avian metapneumovirus( a MPV) JC strain was propagated in Vero cells. The virus titer reached to 105. 6 TCID5 0/m L after 50 passages. Compared to parental virus,the morbidity rate decreased and the pathological changes were alleviated in chicken after being challenged with passage 50 virus. It indicated that the virulence of F50 cell adapted strain was attenuated.
引文
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[15]Saif Y M.禽病学[M].苏敬良,高福,索勋,译.12版.北京:中国农业出版社,2012:110-122.
[2]Cook J K.Avian pneumovirus infections of turkeys and chickens[J].Vet J,2000,160:118-125.
[3]Velayudhan B T,Lopes V C,Noll S L,et al.Avian pneumovirus and its survival in poultry litter[J].Avian Dis,2003,47:764-768.
[4]Buys S B,Du Preez J H.A preliminary report on the isolation of a virus causing sinusitis in turkeys in South African and attempts attenuate the virus[J].Turkeys,11980,28:36-46.
[5]沈瑞忠,曲立新,于康震,等.禽肺病毒的分离鉴定[J].中国预防兽医学报,1999,21(1):76-77.
[6]郭龙宗,曲立新.种鸡禽肺病毒感染的血清学调查[J].中国畜牧兽医,2009,36(4):149-150.
[7]王菁,韦莉,朱珊珊,等.禽APV感染情况初步调查[C].中国畜牧兽医学会禽病学分会第十五次学术研讨会论文集.重庆:中国畜牧兽医学会禽病学分会,2010:141-142.
[8]贠炳岭,刘在斯,吴关,等.我国部分地区种鸡禽肺病毒感染的血清学调查[J].中国家禽,2012,34(12):64-65.
[9]李亚林,段晓琴.新疆昌吉地区规模化种鸡场禽偏肺病毒感染情况的血清学调查[J].当代畜牧,2013(4月中旬刊):72-73.
[10]徐仕忠,秦云,于鹏,等.中国鸡群禽偏肺病毒感染的流行病学调查报告.中国家禽,2013,35(6):59-60.
[11]薛聪,唐熠,陈琳,等.1株B亚型禽偏肺病毒的分离与鉴定[J].中国兽医学报,2014,34(1):39-44.
[12]Wei L,Zhu S,Yan X,et al.Avian metapneumovirus subgroup C infection in chickens,China[J].Emerg Infect Dis,2013,19(7):1 092-1 094.
[13]Patnayak D P,Tiwari A,Goyal S M.Growth of vaccine strains of avian pneumovirus in different cell lines[J].Avian Pathol,2005,34(2):123-126.
[14]Banet-Noach C,Simanov L,Perk S.Characterization of Israeli avian metapneumovirus strains in turkeys and chickens[J].Avian Pathol,2005,34(3):220-226.
[15]Saif Y M.禽病学[M].苏敬良,高福,索勋,译.12版.北京:中国农业出版社,2012:110-122.