2014年春季济南市活禽交易市场与饲养环境禽流感病毒污染情况调查
|
摘要
目的了解济南市禽类交易市场与饲养环境禽流感病毒污染状况,为防控人感染禽流感提供科学依据。方法 2014年在流感高发的春季采集活禽交易市场和养殖场所环境标本,用实时荧光定量PCR法(Real—time PCR)检测禽流感病毒FIu A、H5、H9、H7、N1、N2、N9。结果活禽市场H5N1检出率为25.32%,其中粪便标本H5H1检出率为24.14%,禽类污水H5H1检出率为25.00%,涂抹笼具表面H5H1检出率为26.92%,H9、H7未检出。养殖场所H5、H7、H9均未检出阳性标本。结论济南市不同活禽交易市场、饲养环境H5NI禽流感病毒污染存在差异。
Objectives To determine the pollution of avian influenza virus in the environment of live poultry markets and poultry farms in Jinan in the spring of 2014,and to provide scientific strategies for the prevention and control of avian influenza in human. Methods Samples were collected from the environment in live poultry markets and poultry farms,and the samples were tested by real-time fluorescence quantitative PCR. Results No positive sample for H5,H9 and H7 was detected in poultry farms. The positive rate for H5N1 in poultry markets was 25. 32%,and the positive rate for H5H1 in the excrement of poultry was 24. 41%,in sewages was 25. 00%,and on the surface of cages for keeping poultry was 26. 92%. Conclusions The pollution of H5N1 virus was various in different live poultry markets and poultry farms.
Objectives To determine the pollution of avian influenza virus in the environment of live poultry markets and poultry farms in Jinan in the spring of 2014,and to provide scientific strategies for the prevention and control of avian influenza in human. Methods Samples were collected from the environment in live poultry markets and poultry farms,and the samples were tested by real-time fluorescence quantitative PCR. Results No positive sample for H5,H9 and H7 was detected in poultry farms. The positive rate for H5N1 in poultry markets was 25. 32%,and the positive rate for H5H1 in the excrement of poultry was 24. 41%,in sewages was 25. 00%,and on the surface of cages for keeping poultry was 26. 92%. Conclusions The pollution of H5N1 virus was various in different live poultry markets and poultry farms.
引文
[1]徐淑慧,于秋燕,成洪旗,等.济南市首例人禽流感疫情处置的思考[J].中国预防医学杂志,2010,11(7):724-726.
[2]向妮娟,周蕾,怀扬,等.2005—2009年中国人禽流感(H5N1)病例流行病学特征分析[J].实用预防医学,2010,17(6):1070-1073.
[3]何为涛,谭毅,闭福银,等.2010—2011年广西活禽市场外环境禽流感病毒污染调查[J].应用预防医学,2013,19(1):6-8.
[4]张如胜,欧新华,宋克云,等.长沙市家禽市场环境病毒传播风险研究[J].中华流行病学杂志,2012,33(8):771-773.
[5]方正斌,崔小波,王配强,等.2011年郧县外环境禽流感病原学监测结果分析[J].预防医学情报杂志,2013,29(2):157-159.
[6]姜里迦,黄贵彪,潘丽,等.梧州市2009—2011年禽类环境标本禽流感病毒调查[J].中国热带医学,2013,13(2):180-183.
[7]王军,杨国丽,关乃鹏,等.正确认识和科学防控高致病性禽流感[J].现代畜牧兽医,2012,10(3):33-35.
[8]费强.禽流感疫苗的使用方法[J].今日畜牧兽医,2012(11):52-53.
[2]向妮娟,周蕾,怀扬,等.2005—2009年中国人禽流感(H5N1)病例流行病学特征分析[J].实用预防医学,2010,17(6):1070-1073.
[3]何为涛,谭毅,闭福银,等.2010—2011年广西活禽市场外环境禽流感病毒污染调查[J].应用预防医学,2013,19(1):6-8.
[4]张如胜,欧新华,宋克云,等.长沙市家禽市场环境病毒传播风险研究[J].中华流行病学杂志,2012,33(8):771-773.
[5]方正斌,崔小波,王配强,等.2011年郧县外环境禽流感病原学监测结果分析[J].预防医学情报杂志,2013,29(2):157-159.
[6]姜里迦,黄贵彪,潘丽,等.梧州市2009—2011年禽类环境标本禽流感病毒调查[J].中国热带医学,2013,13(2):180-183.
[7]王军,杨国丽,关乃鹏,等.正确认识和科学防控高致病性禽流感[J].现代畜牧兽医,2012,10(3):33-35.
[8]费强.禽流感疫苗的使用方法[J].今日畜牧兽医,2012(11):52-53.