浙江省缙云县H7N9禽流感外环境标本检测结果分析
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摘要
目的通过对本地H7N9禽流感外环境标本的检测分析,为制定人感染H7N9禽流感防控工作提供参考依据。方法在禽类交易市场、养殖场等地采集禽类相关标本,并采集与H7N9阳性禽类有密切接触史的职业暴露人员的咽拭子;采用实时荧光定量PCR法进行H7N9禽流感病毒核酸检测,并对结果进行统计分析。结果共采集禽类相关标本302份,H7N9阳性率为9.27%。外省调入、外县调入、本地散养及本地养殖场4种不同来源的禽类中,以外省调入禽类的阳性率最高(25.64%),差异有统计学意义(χ2=19.99,P=0.000)。鸭、鹅、鸡及鸽4种禽类中,以鸭相关标本的阳性率最高(16.67%),差异有统计学意义(χ2=13.49,P=0.002<0.05)。5种不同类型标本中,禽类饮水与粪便标本的阳性率差异有统计学意义(χ2=4.40,P=0.036<0.05)。22份与H7N9阳性禽类有密切接触史的职业暴露人员的咽拭子结果均为阴性。结论本县禽类中存在H7N9禽流感病毒的感染,以外省调运进入的鸭群感染率最高。高危职业人群存在一定风险,应重点做好健康宣教和日常监测工作。
Objective Through the detection and analysis of the local H7N9 bird flu environmental samples,to provide reference for the human- infected H7N9 avian influenza prevention and control work. Methods In poultry farms and other trading markets,to collect relevant specimens and swabs from occupation exposure personnel with close contact history with H7N9 positive birds; H7N9 avian influenza virus nucleic acid was detected by real- time fluorescent quantitative PCR,and then the test results were statistically analyzed. Results A total of 302 samples were collected,the positive rate of H7N9 was 9. 27%. Among the 4 different sources of birds from other provinces transferred,the county transferred,local backyard and local farms,the highest positive rate of poultry was from the other provinces transferred( 25. 64%),the difference was statistically significant( χ2= 19. 99,P = 0. 000). Among the 4 kinds of birds,duck,goose,chicken and pigeon,the highest positive rate was from duck specimens( 16. 67%),the difference was statistically significant( χ2= 13. 49,P = 0. 002 < 0. 05). Among the 5types of specimens,the differences on the positive rates of poultry drinking water and feces samples were statistically significant( χ2= 4. 40,P = 0. 036 < 0. 05). The swab results of 22 occupation exposure personnel with close contact history with H7N9 positive birds were negative. Conclusion The presence of H7N9 avian influenza virus in poultry infection occurs in our county,provincial transportation ducks were with the highest infection rate. Certain risks exist high- risk occupation groups,we should focus on good health education and routine monitoring.
Objective Through the detection and analysis of the local H7N9 bird flu environmental samples,to provide reference for the human- infected H7N9 avian influenza prevention and control work. Methods In poultry farms and other trading markets,to collect relevant specimens and swabs from occupation exposure personnel with close contact history with H7N9 positive birds; H7N9 avian influenza virus nucleic acid was detected by real- time fluorescent quantitative PCR,and then the test results were statistically analyzed. Results A total of 302 samples were collected,the positive rate of H7N9 was 9. 27%. Among the 4 different sources of birds from other provinces transferred,the county transferred,local backyard and local farms,the highest positive rate of poultry was from the other provinces transferred( 25. 64%),the difference was statistically significant( χ2= 19. 99,P = 0. 000). Among the 4 kinds of birds,duck,goose,chicken and pigeon,the highest positive rate was from duck specimens( 16. 67%),the difference was statistically significant( χ2= 13. 49,P = 0. 002 < 0. 05). Among the 5types of specimens,the differences on the positive rates of poultry drinking water and feces samples were statistically significant( χ2= 4. 40,P = 0. 036 < 0. 05). The swab results of 22 occupation exposure personnel with close contact history with H7N9 positive birds were negative. Conclusion The presence of H7N9 avian influenza virus in poultry infection occurs in our county,provincial transportation ducks were with the highest infection rate. Certain risks exist high- risk occupation groups,we should focus on good health education and routine monitoring.
引文
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[9]胡红蔚,刘亭亭,黄大馄,等.浙江省永嘉县人感染H7N9禽流感外环境监测分析[J].疾病监测,2014,29(10):802-805.
[10]陈恩富,柴程良,孙继民,等.浙江省人感染H7N9禽流感流行特征与防控对策[J].中国公共卫生,2013,29(5):625-627.
[11]曹国平,占炳东,陈旭富,等.浙江省衢州市禽类职业暴露人群及外环境禽流感病毒监测分析[J].疾病监测,2013,28(11):884-887.
[12]崔小波,王定芳,力正斌,等.2011年郧县禽流感高危职业人群及外环境监测结果分析[J].实用预防医学,2012,19(2):252-253.
[2]Chen Y,Liang W,Yang S,et al.Human infections with the emerging avian influenza A H7N9 virus from wet market poultry:clinical analysis and characterization of viral genome[J].Lancetm,2013,381(9881):1916-1925.
[3]Fang LQ,Li XL,Liu K,et al.Mapping spread and risk of avian influenza A(H7N9)in China[J].Sci Rep,2013,26(3):2722.
[4]Ai J,Huang Y,Xu K,et al.Case-control study of risk factors for human infection with influenza A(H7N9)virus in Jiangsu province,China,2013[J].Euro Surveill,2013,18(26):20510.
[5]雷永良,叶夏良,陈秀英,等.浙江省丽水市人感染H7N9禽流感病毒监测结果分析[J].疾病监测,2014,29(9):693-695.
[6]李羽敏,董升草,应丽红.浙江省丽水市人感染H7N9禽流感病例流行病学调查[J].疾病监测,2014,29(6):502-503.
[7]寿钧,周晓红,何玉芳,等.杭州市下城区4例人感染H7N9禽流感病例的临床与流行病学特征分析[J].疾病监测,2013,28(8):657-659.
[8]刘菊辉,何峰,周仲南,等.宁乡县家禽养殖环境禽流感病毒监测分析[J].实用预防医学,2012,19(11):1724-1726.
[9]胡红蔚,刘亭亭,黄大馄,等.浙江省永嘉县人感染H7N9禽流感外环境监测分析[J].疾病监测,2014,29(10):802-805.
[10]陈恩富,柴程良,孙继民,等.浙江省人感染H7N9禽流感流行特征与防控对策[J].中国公共卫生,2013,29(5):625-627.
[11]曹国平,占炳东,陈旭富,等.浙江省衢州市禽类职业暴露人群及外环境禽流感病毒监测分析[J].疾病监测,2013,28(11):884-887.
[12]崔小波,王定芳,力正斌,等.2011年郧县禽流感高危职业人群及外环境监测结果分析[J].实用预防医学,2012,19(2):252-253.