不同分子量的腐殖酸存在下2种无机砷的生物毒性效应研究
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摘要
水环境中天然有机质会与砷形成络合物,进而影响砷的迁移、转化和生物毒性。研究利用超滤方法将腐殖酸(humic acid, HA)分为5个不同分子量的组分,以大型溞为受试生物,探究了不同分子量HA存在下砷对大型溞的毒性效应。结果表明,不同分子量的HA均缓解了As(Ⅲ)和As(Ⅴ)对大型溞的氧化应激损伤和细胞膜损伤,并降低了砷对MT的诱导量。其中1～30 k Da的HA对砷的缓解效果最好,<1 k Da的HA毒性缓解效果最差,可能的原因是HA与砷的络合增加溶液中络合态砷的含量,而络合态砷难以进入细胞并被生物利用。不同分子量的HA对砷毒性的缓解差异与其跟砷的络合比例不同有关。
Natural organic matter(DOM) may affect the migration, transformation and bioavailability of arsenic in aquatic environment by forming As-DOM complex. Using Daphnia magna as the case, humic acid(HA) was divided into 5 groups by ultrafiltration to determine the impacts from different molecular weights on arsenic toxicity in aquatic environment. The results indicated that all HA fractions can significantly alleviate the oxidative stress and membrane damage induced by As, and in the meanwhile reduce MT concentration. In particular, HA from 1-30 kDa fraction had the most remarkable alleviation effect, while the alleviation effect of HA(<1 kDa) was the lowest.The possible reason is that the complexation of HA with arsenic could increase As-DOM concentration in exposed medium, which is difficult to enter cells and be assimilated by organism. The effects of HA in alleviating arsenic toxicity with different molecular weights were related to complexation ratios.
Natural organic matter(DOM) may affect the migration, transformation and bioavailability of arsenic in aquatic environment by forming As-DOM complex. Using Daphnia magna as the case, humic acid(HA) was divided into 5 groups by ultrafiltration to determine the impacts from different molecular weights on arsenic toxicity in aquatic environment. The results indicated that all HA fractions can significantly alleviate the oxidative stress and membrane damage induced by As, and in the meanwhile reduce MT concentration. In particular, HA from 1-30 kDa fraction had the most remarkable alleviation effect, while the alleviation effect of HA(<1 kDa) was the lowest.The possible reason is that the complexation of HA with arsenic could increase As-DOM concentration in exposed medium, which is difficult to enter cells and be assimilated by organism. The effects of HA in alleviating arsenic toxicity with different molecular weights were related to complexation ratios.
引文
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[21] Zhao C M, Campbell P G C, Wilkinson K J. When are metal complexes bioavailable[J]. Environmental Chemistry, 2016, 13(3):425-433
[22] Shen S, Li X F, Cullen W R, et al. Arsenic binding to proteins[J]. Chemical Reviews, 2013, 113(10):7769-7792
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[25] Ren J Q, Fan W H, Wang X R, et al. Influences of sizefractionated humic acids on arsenite and arsenate complexation and toxicity to Daphnia magna[J]. Water Research, 2017, 108:68-77
[2] Kumari B, Kumar V, Sinha A K, et al. Toxicology of arsenic in fish and aquatic systems[J]. Environmental Chemistry Letters, 2017, 15(1):43-64
[3] Sharma V K, Sohn M. Aquatic arsenic:Toxicity, speciation, transformations, and remediation[J]. Environment International, 2009, 35(4):743-759
[4] Flora S J. Arsenic-induced oxidative stress and its reversibility[J]. Free Radical Biology&Medicine, 2011, 51(2):257-281
[5] Kharroubi W, Haj Ahmed S, Nury T, et al. Mitochondrial dysfunction, oxidative stress and apoptotic induction in microglial BV-2 cells treated with sodium arsenate[J].Journal of Environmental Sciences, 2017, 51:44-51
[6] Yan C Z, Yang F, Wang Z S, et al. Changes in arsenate bioaccumulation, subcellular distribution, depuration, and toxicity in Artemia salina nauplii in the presence of titanium dioxide nanoparticles[J]. Environmental ScienceNano, 2017, 4(6):1365-1376
[7]王亚军,马军.水体环境中天然有机质腐殖酸研究进展[J].生态环境学报, 2012, 21(6):1155-1165Wang Y J, Ma J. The research progress of humic acid in aquatic environment[J]. Ecology and Environmental Sciences, 2012, 21(6):1155-1165(in Chinese)
[8]吴丰昌,王立英,黎文,等.天然有机质及其在地表环境中的重要性[J].湖泊科学, 2008, 20(1):1-12Wu F C, Wang L Y, Li W, et al. Natural organic matter and its significance in terrestrial surface environment[J].Lake Sciences, 2008, 20(1):1-12(in Chinese)
[9] Chappaz A, Curtis P J. Integrating empirically dissolved organic matter quality for WHAM VI using the DOM optical properties:A case study of Cu-Al-DOM interactions[J]. Environmental Science&Technology, 2013, 47(4):2001-2007
[10]刘广良,蔡勇.环境中砷与溶解有机质的络合作用[J].环境化学, 2011, 30(1):50-55Liu G L, Cai Y. Complexation of arsenic with dissolved organic matter in environment[J]. Environmental Chemistry, 2011, 30(1):50-55(in Chinese)
[11] Dutton J, Fisher N S. Influence of humic acid on the uptake of aqueous metals by the killifish Fundulus heteroclitus[J]. Environmental Toxicology and Chemistry, 2012,31(10):2225-2232
[12] Zhang J Y, Ni Y Y, Ding T D, et al. The role of humic acid in the toxicity of arsenite to the diatom Navicula sp[J]. Environmental Science and Pollution Rresearch, 2014,21(6):4366-4375
[13] Al-Reasi H A, Smith D S, Wood C M. Evaluating the ameliorative effect of natural dissolved organic matter(DOM)quality on copper toxicity to Daphnia magna:Improving the BLM[J]. Ecotoxicology, 2012, 21(2):524-537
[14] Al-Reasi H A, Wood C M, Smith D S. Physicochemical and spectroscopic properties of natural organic matter(NOM)from various sources and implications for ameliorative effects on metal toxicity to aquatic biota[J]. Aquatic Toxicology, 2011, 103(3):179-190
[15] ISO. Water Quality. Determination of the Acute Toxicity of Substance to Daphnia(Daphnia magna Strauss). ISO6341.[S]. Geneva:ISO, 1982
[16] Wang X D, Chen X N, Liu S A, et al. Effect of molecular weight of dissolved organic matter on toxicity and bioavailability of copper to lettuce[J]. Journal of Environmental Sciences, 2010, 22(12):1960-1965
[17] Poldoski J E. Cadmium bioaccumulation assays. Their relationship to various ionic equilibriums in Lake Superior water[J]. Environmental Science&Technology, 1980, 14(6):735
[18] Fan W H, Yang X P, Shi Z W, et al. Effect of humic acid on copper uptake and metallothionein induction in Daphnia magna[J]. Fresennius Environmental Bulletin, 2012,21(8):2056-2061
[19] Lorenzo J I, Nieto O, Beiras R. Effect of humic acids on speciation and toxicity of copper to Paracentrotus lividus larvae in seawater[J]. Aquatic Toxicology, 2002, 58(1):27-41
[20]王利红,尹西翔,段桂兰,等.生物体砷代谢解毒机制的研究进展[J].安徽农业科学, 2009, 17(17):8144-8147Wang L H, Yin X X, Duan G L, et al. Developments in understanding mechanisms of arsenic metabolism and detoxication in organisms[J]. Anhui Agriculture Science,2009, 17(17):8144-8147(in Chinese)
[21] Zhao C M, Campbell P G C, Wilkinson K J. When are metal complexes bioavailable[J]. Environmental Chemistry, 2016, 13(3):425-433
[22] Shen S, Li X F, Cullen W R, et al. Arsenic binding to proteins[J]. Chemical Reviews, 2013, 113(10):7769-7792
[23] Ajees A A, Rosen B P. As(III)S-adenosylmethionine methyltransferases and other arsenic binding proteins[J].Geomicrobiology Journal, 2015, 32(7):570-576
[24] Roggenbeck B A, Banerjee M, Leslie E M. Cellular arsenic transport pathways in mammals[J]. Journal of Environmental Sciences, 2016, 49(11):38-58
[25] Ren J Q, Fan W H, Wang X R, et al. Influences of sizefractionated humic acids on arsenite and arsenate complexation and toxicity to Daphnia magna[J]. Water Research, 2017, 108:68-77