摘要
目的:建立常用秋海棠属中药材水八角及红白二丸质量评价方法,为该属药材的质量评价提供依据。方法:对水八角和红白二丸的芦丁含量及HPLC指纹图谱,进行比较研究。结果:芦丁含量在水八角中范围为0.022%~0.207%,在红白二丸中范围为0.001%~0.019%;水八角指纹图谱有10个共有峰,9批样品90%相似度在0.9以上;红白二丸指纹图谱有13个共有峰,19批样品68.4%相似度在0.9以上。结论:掌裂叶秋海棠含有的化学成分与秋海棠、中华秋海棠的区别大,秋海棠与中华秋海棠所含有的化学成分差异小。本研究建立的秋海棠属药材质量评价方法,可用于秋海棠属中药材的整体质量控制,为该属药材的合理利用以及资源开发奠定基础。
Objective: To establish the quality detection methods for Gratiola offcinalis L. and Begeonia sinensis A. DC. and provide basis for the quality evaluation of Begonia Linn.. Methods: The content of rutin was detected and the HPLC fingerprint was established for Gratiola offcinalis L. and Begeonia sinensis A. DC.. Results: The rutin content in Gratiola offcinalis L. was within the range of 0. 022%-0. 207% and that in Begeonia sinensis A. DC. was within the range of 0. 001%-0. 019%. The HPLC fingerprint of Gratiola offcinalis L. had ten common peaks and the similarity of 90% samples reached over 0. 9. The HPLC fingerprint of Begeonia sinensis A. DC. had thirteen common peaks and the similarity of 68. 4% samples reached over 0. 9. Conclusion: The chemical compositions contained in B. pedatifida Levl. are different from those in B. sinensis A. DC. and B. evansiana Andr. The differences in the chemical compositions between B. sinensis A. DC. and B. evansiana Andr are very small. The quality evaluation methods can be used for the rational utilization and development of Begonia Linn.
引文
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