中红杨离体叶片植株再生体系建立
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摘要
以中红杨组培苗的叶片为试验材料,研究了基本培养基、植物生长调节物质浓度、外源添加物和光照条件对离体叶片不定芽再生的影响,并获得了完整的再生植株。结果表明:1)最适基本培养基为MS,最佳植物生长调节剂和外源添加物的组合为0.5 mg·L~(-1) 6-BA+0.2 mg·L~(-1) NAA+30 g·L~(-1)蔗糖;2)暗培养有利于愈伤组织诱导,光照16 h·d~(-1)有利于不定芽的诱导,不定芽形成率可达83.3%;3)将叶片再生植株转接到MS+0.2 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA培养基中壮苗培养,在1/2 MS+0.2 mg·L~(-1) IBA+20 g·L~(-1)蔗糖+6.5 g·L~(-1)琼脂培养基诱导生根,生根率96.4%,生根苗大田移栽成活率86.8%。
Taking the leaves of Populus × euramericana ‘zhonghong' as tested materials, the factors affecting in vitro leaf regeneration, including basal medium, plant growth regulator, exogenous additives and illumination conditions, were studied, and some complete regenerated plants were obtained, and a high ef?cient system of in vitro leaf regeneration was established. The results show that: 1) The optimum basic medium was MS, and the combination of the optimal plant growth regulator and the exogenous additive was0.5 mg·L~(-1) 6-BA + 0.2 mg·L~(-1) NAA + 30 g·L~(-1) sucrose; 2) Dark culture was conducive to callus induction, illumination of 16 h·d~(-1) was conducive to adventitious bud induction, adventitious bud formation rate could reach 83.3%; 3) Leaf regenerated plants were transferred to MS + 0.2 mg·L~(-1) 6-BA + 0.1 mg·L~(-1) NAA medium for strong seedling culture, the leaves were induced and rooted in 1/2 MS + 0.2 mg·L~(-1) IBA + 20 g·L~(-1) sucrose + 6.5 g·L~(-1) agar medium, the rooting rate was 96.4%, and the survival rate of rooting seedlings in the big ?eld was 86.8%.
Taking the leaves of Populus × euramericana ‘zhonghong' as tested materials, the factors affecting in vitro leaf regeneration, including basal medium, plant growth regulator, exogenous additives and illumination conditions, were studied, and some complete regenerated plants were obtained, and a high ef?cient system of in vitro leaf regeneration was established. The results show that: 1) The optimum basic medium was MS, and the combination of the optimal plant growth regulator and the exogenous additive was0.5 mg·L~(-1) 6-BA + 0.2 mg·L~(-1) NAA + 30 g·L~(-1) sucrose; 2) Dark culture was conducive to callus induction, illumination of 16 h·d~(-1) was conducive to adventitious bud induction, adventitious bud formation rate could reach 83.3%; 3) Leaf regenerated plants were transferred to MS + 0.2 mg·L~(-1) 6-BA + 0.1 mg·L~(-1) NAA medium for strong seedling culture, the leaves were induced and rooted in 1/2 MS + 0.2 mg·L~(-1) IBA + 20 g·L~(-1) sucrose + 6.5 g·L~(-1) agar medium, the rooting rate was 96.4%, and the survival rate of rooting seedlings in the big ?eld was 86.8%.
引文
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[11]秦公伟,曹小勇,张静,等.银杏叶片愈伤组织培养及光暗条件对黄铜含量的影响[J].种子,2017,27(1):71-76.
[12]蒲秀琴,薛寒青.百合组培苗移栽前的壮苗技术研究[J].青海农林科技,2007(1):59-60.
[2]程相军.中华红叶杨(中红杨)的科学研究与发展前景[J].现代园林,2006(5):31-32.
[3]李小康,朱延林,宁豫婷,等.不同光照条件下外施营养液对中红杨叶色变化的影响[J].上海农业学报,2008,24(2):20-24.
[4]许鹏.中华红叶杨栽培技术[J].山西林业,2010(1):22-23.
[5]韩富军.中华红叶杨在兰州地区的引种试验[J].青海大学学报(自然科学版),2009,27(1):79-81,85.
[6]王欢,许士钊,李旭.长白忍冬的组织培养与快速繁殖[J].北方园艺,2016,40(13):107-110.
[7]赵振利,曹艳春,刘荣宁.素心腊梅组织培养及其体外植株再生技术[J].北方园艺,2017(3):112-115.
[8]张文泉,邓洁,罗国涛,等.青钱柳组织培养研究[J].中南林业科技大学学报,2018,38(3):13-19.
[9]高红兵,唐晓杰,孟庆繁.高浓度6-BA诱导酸樱桃苗的玻璃化苗内源激素含量变化[J].林业科学研究,2006,19(4):488-490.
[10]潘梅,符瑞侃,戚华莎,等.黄花马铃苣苔不定芽高频再生研究[J].现代农业科技,2017,36(1):85-89.
[11]秦公伟,曹小勇,张静,等.银杏叶片愈伤组织培养及光暗条件对黄铜含量的影响[J].种子,2017,27(1):71-76.
[12]蒲秀琴,薛寒青.百合组培苗移栽前的壮苗技术研究[J].青海农林科技,2007(1):59-60.