高效混合石油降解菌种的制备
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摘要
经过分离、纯化、富集等操作,从咸阳某石化公司石油废水中分离了5种降解效果好的菌种。通过对这些菌种形态和生理特性进行分析,鉴别菌种到属。研究降解效率及菌种数量随时间变化的关系,探索单个菌种降解趋势。然后把单一菌种按不同比例混合,探索降解效果最优的复合菌种投加比例。实验结果表明:W_1为奈瑟氏球菌属,W_2和W_5为枯叶芽孢杆菌,W_3为假单胞菌属,W_4为动胶菌属,都是革兰氏阴性菌。随着时间的延长,单一菌种的降解效果逐渐增加,其中以W_3的降解效果最好,达到了40.60%。把不同的菌种进行正交实验,发现菌种最优投加比例为W_1∶W_2∶W_3∶W_4∶W_5=1∶0.5∶0.5∶1∶0.5时,混合菌种降解效果最好,达到了75.43%。
After separation, purification, enrichment and other operations, five kinds of good bacteria were isolated from petrochemical wastewater of a Xianyang petrochemical company.Through the analysis of morphology and physiological characteristics of bacteria, their species were identified. Then the relationship between the degradation efficiency and the number of bacteria species with time was studied, and the trend of degradation of individual strains was investigated. Then single strains were mixed in different proportions to explore the composite strain suitable dosing ratio in order to obtain the best degradation effect. The experimental results show that W_1 is Neisseria, W_2 and W_5 are Bacillus thuringiensis, W_3 is Pseudomonas,W_4 is Agrobacterium,all of which are Gram-negative bacteria.With the extension of time, the degradation effect of bacteria gradually increased, and the degradation effect of W_3 was the best, reaching 40.60%.By orthogonal experiment, the optimum ratio of the species was found as follows: W_1∶W_2∶W_3∶W_4∶W_5 = 1∶0.5∶0.5∶1∶0.5, the mixed strain had the best degradation effect, reaching 75.43%.
After separation, purification, enrichment and other operations, five kinds of good bacteria were isolated from petrochemical wastewater of a Xianyang petrochemical company.Through the analysis of morphology and physiological characteristics of bacteria, their species were identified. Then the relationship between the degradation efficiency and the number of bacteria species with time was studied, and the trend of degradation of individual strains was investigated. Then single strains were mixed in different proportions to explore the composite strain suitable dosing ratio in order to obtain the best degradation effect. The experimental results show that W_1 is Neisseria, W_2 and W_5 are Bacillus thuringiensis, W_3 is Pseudomonas,W_4 is Agrobacterium,all of which are Gram-negative bacteria.With the extension of time, the degradation effect of bacteria gradually increased, and the degradation effect of W_3 was the best, reaching 40.60%.By orthogonal experiment, the optimum ratio of the species was found as follows: W_1∶W_2∶W_3∶W_4∶W_5 = 1∶0.5∶0.5∶1∶0.5, the mixed strain had the best degradation effect, reaching 75.43%.
引文
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[16]宁卓,刘雅慈,张胜,等.石油降解菌的分离筛选及混合菌群的构建与优化[J].四川环境,2013,32(6):16-20.
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[3]郭璇,唐翠梅,赵春彦.浅谈国内石化废水深度处理回用技术应用现状[J].环境与可持续发展,2015,40(5):75-77.
[4]刘振东.海洋石油污染与生物修复[J].中国水运(理论版),2006,4(11):17-18.
[5]周楚莹.石油降解菌群的构建及其固定化技术研究[D].广东工业大学,2015.
[6]李宝明,阮志勇,姜瑞波.石油降解菌的筛选、鉴定及菌群构建[J].中国土壤与肥料,2007,2007(3):68-72.
[7]谢丹平,尹华,彭辉.石油降解菌株的分离及其降解特性研究[J].上海环境科学,2003(12):951-954.
[8]汪杰,郑维爽,礼晓,等.高效石油降解菌的筛选鉴定及修复能力研究[J].环境科学学报,2010,30(6):1228-1234.
[9]王鑫,王学江,刘免,等.高效石油降解菌群构建及降解性能[J].海洋环境科学,2014,33(4):576-579.
[10]Chen J,Yang Q Y,Huang T P,et al.Enhanced bioremediation of soil contaminated with viscous oil through microbial consortium construction and ultraviolet mutation[J].World Journal of Microbiology and Biotechnology,2011,27(6):1381-1389.
[11]范瑞娟,郭书海,李凤梅.石油降解菌群的构建及其对混合烃的降解特性[J].农业环境科学学报,2017,36(3):522-530.
[12]陈晓鹏,易筱筠,陶雪琴,等.石油污染土壤中芘高效降解菌群的筛选及降解特性研究[J].环境工程学报,2008,2(3):413-417.
[13]Lal B,Khanna S.Degradation of crude oil by Acinetobacter calcoaceticus and Alcaligenes odorans[J].Journal of Applied Bacteriology,1996,81(4):355-62.
[14]Tao X Q,Lu G N,Dang Z,et al.A phenanthrene-degrading strain Spingomonas sp.GY2B isolated from contaminated soils[J].Process Biochemistry,2007,42(3):401-408.
[15]李丽,张利平,张元亮.石油烃类化合物降解菌的研究概况[J].微生物学通报,2001,28(5):89-92.
[16]宁卓,刘雅慈,张胜,等.石油降解菌的分离筛选及混合菌群的构建与优化[J].四川环境,2013,32(6):16-20.