蛹虫草菌种退化的探讨
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摘要
蛹虫草具有与冬虫夏草极相似的药理药效,可以作为冬虫夏草的代用品,但其菌株在继代培养过程中极易退化导致产量急剧下降,是影响行业发展的核心技术问题。对蛹虫草退化菌种形态学、遗传学及子实体变化,并对当前研究中存在问题及解决菌种退化的方法进行探讨。
Comparing with the function pharmacology and drug effect,Cordyceps militaris was similar with Cordyceps sinensis,which had been provided substitution of Cordyceps sinensis. However,the degenerate subcultures of C. militaris had increasingly reduced the production of this economically important fungus. In this paper,the development progresses of Cordyceps militaris degenerate subculture in morphological,genetic and changes fruit bodies changes were summed up. At the same time,we prospect the current research existence questions and the technique solution of degenerate subculture.
Comparing with the function pharmacology and drug effect,Cordyceps militaris was similar with Cordyceps sinensis,which had been provided substitution of Cordyceps sinensis. However,the degenerate subcultures of C. militaris had increasingly reduced the production of this economically important fungus. In this paper,the development progresses of Cordyceps militaris degenerate subculture in morphological,genetic and changes fruit bodies changes were summed up. At the same time,we prospect the current research existence questions and the technique solution of degenerate subculture.
引文
[1]汪虹,魏静,林楠.交配型基因作为分子标记鉴定蛹虫草退化菌株的核相初步研究[J].食用菌学报,2010,17(4):1-4.
[2]杨涛,董彩虹.虫草素的研究开发现状与思考[J].菌物学报,2011,30(2):180-190.
[3]郭成金,赵润,朱文碧.冬虫夏草与蛹虫草原生质体融合初探[J].食品科学,2010,31(1):165-171.
[4]朱子雄,谢放,张楠.甘肃省冬虫夏草遗传多样性的ISSR分析[J].菌物学报,2011,30(3):501-507.
[5]李光荣,文庭池,康冀川,等.蛹虫草表型多态性对子实体产生及虫草菌素的影响[J].微生物学通报,2011,38(3):370-382.
[6]李学军,都兴范,王林美,等.蛹虫草中虫草素的提取及纯化工艺研究[J].生物技术,2012,22(5):75-78.
[7]李美娜,吴谢军,李春燕.人工栽培蛹虫草退化现象的分子分析[J].菌物系统,2003,22(2):277-282.
[8]Li L,Pischetsrieder M,J St Leger R,et al.Associated links among mt DNA glycation,oxidative stress and colony sectorization in Metarhizium anisopliae[J].Fungal Genetics and Biology,2008,45(9):1300-1306.
[9]丁湖广.菌种退化与老化原因及防止措施[J].特种经济动植物,2006(1):39-40.
[10]Magae Y,Hayashi N.Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulima velutipes(Enokitake)[J].FEMS Microbiology Letters,1999(180):331-335.
[11]Hemachandra L,Paul H,Victoria F,et al.A new insertion sequence,ISCb1,from Clostridium beijerinckii NCIMB 8052[J].Journal of Molecular Microbiology and Biotechnology,2000(2):107-113.
[12]Chen CK,Blaschek HP.Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB8052solvent production and strain degeneration[J].Applied&Environmental Microbiology,1999(65):499-505.
[13]林清泉,丘雪红,郑壮丽,等.蛹虫草退化菌株的特征研究[J].菌物学报,2010,29(5):670-677.
[14]王英,庄南生,高和琼,等.甘蔗种质遗传基础的ISSR分析[J].湖南农业大学学报:自然科学版,2007(Z1):176-182.
[15]Wang CS,Butt TM,St Leger RJ.Colony sectorization of Metarhizium anisopliae is a sign of ageing[J].Microbiology,2005(151):3223-3236.
[16]唐晓庆,樊美珍.球孢白僵菌继代培养中菌落局变现象及环境影响因素的研究[J].真菌学报,1996,15(3):188-196.
[17]梁宗琦.蛹虫草无性型——蛹草拟青霉的确证[J].食用菌学报,2001,8(4):28-32.
[18]梁宗琦,Roland TVF.蛹虫草(Cordyceps militaris)无性型的多型现象[J].菌物系统,1998,17(1):57-62.
[19]高新华,吴畏,钱国琛,等.北冬虫夏草单孢菌株配对后对子实体形成的影响与无性型产孢结构关系[J].上海农业学报,2000(16):85-92.
[20]薛建娥,冉翠香.蛹虫草人工栽培种的分离与复壮[J].中国食用菌,2002,24(2):17-18.
[21]陈永久,宿兵,王文.用RAPD标记冬虫夏草[Cordyceps sinensis(Berk.)Sacc.]个体的遗传特性[J].中国虫生真菌研究与应用,1997a(4):97-101.
[22]陈永久,王文,杨跃雄.冬虫夏草(Cordyceps sinensis)的随机扩增多态DNA及其遗传分化[J].遗传学报,1997b,24(5):410-416.
[23]张云武,陈永久,沈发荣,等.滇西北地区冬虫夏草和阔孢虫草的遗传分化研究[J].菌物系统,1999,18(2):176-183.
[24]梁月,张国珍,安沫平,等.蛹虫草子囊孢子萌发及其后代群体培养性状观察[J].菌物学报,2005,24(4):525-532.
[2]杨涛,董彩虹.虫草素的研究开发现状与思考[J].菌物学报,2011,30(2):180-190.
[3]郭成金,赵润,朱文碧.冬虫夏草与蛹虫草原生质体融合初探[J].食品科学,2010,31(1):165-171.
[4]朱子雄,谢放,张楠.甘肃省冬虫夏草遗传多样性的ISSR分析[J].菌物学报,2011,30(3):501-507.
[5]李光荣,文庭池,康冀川,等.蛹虫草表型多态性对子实体产生及虫草菌素的影响[J].微生物学通报,2011,38(3):370-382.
[6]李学军,都兴范,王林美,等.蛹虫草中虫草素的提取及纯化工艺研究[J].生物技术,2012,22(5):75-78.
[7]李美娜,吴谢军,李春燕.人工栽培蛹虫草退化现象的分子分析[J].菌物系统,2003,22(2):277-282.
[8]Li L,Pischetsrieder M,J St Leger R,et al.Associated links among mt DNA glycation,oxidative stress and colony sectorization in Metarhizium anisopliae[J].Fungal Genetics and Biology,2008,45(9):1300-1306.
[9]丁湖广.菌种退化与老化原因及防止措施[J].特种经济动植物,2006(1):39-40.
[10]Magae Y,Hayashi N.Double-stranded RNA and virus-like particles in the edible basidiomycete Flammulima velutipes(Enokitake)[J].FEMS Microbiology Letters,1999(180):331-335.
[11]Hemachandra L,Paul H,Victoria F,et al.A new insertion sequence,ISCb1,from Clostridium beijerinckii NCIMB 8052[J].Journal of Molecular Microbiology and Biotechnology,2000(2):107-113.
[12]Chen CK,Blaschek HP.Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB8052solvent production and strain degeneration[J].Applied&Environmental Microbiology,1999(65):499-505.
[13]林清泉,丘雪红,郑壮丽,等.蛹虫草退化菌株的特征研究[J].菌物学报,2010,29(5):670-677.
[14]王英,庄南生,高和琼,等.甘蔗种质遗传基础的ISSR分析[J].湖南农业大学学报:自然科学版,2007(Z1):176-182.
[15]Wang CS,Butt TM,St Leger RJ.Colony sectorization of Metarhizium anisopliae is a sign of ageing[J].Microbiology,2005(151):3223-3236.
[16]唐晓庆,樊美珍.球孢白僵菌继代培养中菌落局变现象及环境影响因素的研究[J].真菌学报,1996,15(3):188-196.
[17]梁宗琦.蛹虫草无性型——蛹草拟青霉的确证[J].食用菌学报,2001,8(4):28-32.
[18]梁宗琦,Roland TVF.蛹虫草(Cordyceps militaris)无性型的多型现象[J].菌物系统,1998,17(1):57-62.
[19]高新华,吴畏,钱国琛,等.北冬虫夏草单孢菌株配对后对子实体形成的影响与无性型产孢结构关系[J].上海农业学报,2000(16):85-92.
[20]薛建娥,冉翠香.蛹虫草人工栽培种的分离与复壮[J].中国食用菌,2002,24(2):17-18.
[21]陈永久,宿兵,王文.用RAPD标记冬虫夏草[Cordyceps sinensis(Berk.)Sacc.]个体的遗传特性[J].中国虫生真菌研究与应用,1997a(4):97-101.
[22]陈永久,王文,杨跃雄.冬虫夏草(Cordyceps sinensis)的随机扩增多态DNA及其遗传分化[J].遗传学报,1997b,24(5):410-416.
[23]张云武,陈永久,沈发荣,等.滇西北地区冬虫夏草和阔孢虫草的遗传分化研究[J].菌物系统,1999,18(2):176-183.
[24]梁月,张国珍,安沫平,等.蛹虫草子囊孢子萌发及其后代群体培养性状观察[J].菌物学报,2005,24(4):525-532.