酸柏栀油软胶囊对2型糖尿病肾病大鼠的作用
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摘要
目的探究酸柏栀油软胶囊对糖尿病肾病(DN)大鼠的作用。方法 60只4周龄雄性SD大鼠随机分为正常对照组和模型组,运用高糖高脂饲料联合小剂量链脲佐菌素(STZ)制备DN模型。成模后大鼠随机分为模型对照组、阳性对照组、酸柏栀油软胶囊高、中、低剂量组。正常对照组和模型对照组给予等量生理盐水,阳性对照组给予盐酸二甲双胍、酸柏栀油软胶囊高、中、低剂量组分别给予不同剂量酸柏栀油软胶囊灌胃。干预8 w末,检测大鼠血糖、血脂、血肌酐(SCr)、尿素氮(BUN)水平。结果在干预末,各给药组体重均显著高于模型对照组(P<0.05),酸柏栀油软胶囊高剂量组体重、血糖、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、BUN与阳性对照组相比差异无统计学意义(P>0.05),而与模型组相比差异有统计学意义(P<0.05)。结论酸柏栀油软胶囊具有改善DN大鼠糖代谢、脂代谢紊乱的作用,能促进糖尿病大鼠肾功能的恢复。
Objective To study the effect of soft capsule of compound oil of jujube,arboruitae,and gardenia on diabetic nephropathy rats.Methods Sixty four-week-old male SD rats were randomly divided into normal control and model groups. Diabetic nephropathy model was established by high-sugar and high-fat diet combined with low-dose STZ. The rats were randomly divided into model control, positive control and different dosage groups of compound oil of soft capsule of compound oil of jujube, arboruitae, and gardenia. The normal control and model control groups were given the same amount of saline. The positive control group was given metformin hydrochloride and the intervention groups were given different doses of Soft capsule of compound oil of jujube,arboruitae,and gardenia. After 8 weeks of intervention, the levels of blood glucose, blood lipid, creatinine and urea nitrogen were measured.Results At the end of the intervention, the body weight of each intervention group was higher than that of model control group(P<0.05).There were no significant differences in body weight, blood glucose, HDL-C, LDL-C, TC, BUN between high dose group and positive control group(P>0.05), but there were significant differences between high dose group and model group(P<0.05). Conclusions soft capsule of compound oil of jujube,arboruitae,and gardenia could improve glucose metabolism and lipid metabolism disorders in diabetic nephropathy rats, and promote the recovery of renal function in diabetic rats.
Objective To study the effect of soft capsule of compound oil of jujube,arboruitae,and gardenia on diabetic nephropathy rats.Methods Sixty four-week-old male SD rats were randomly divided into normal control and model groups. Diabetic nephropathy model was established by high-sugar and high-fat diet combined with low-dose STZ. The rats were randomly divided into model control, positive control and different dosage groups of compound oil of soft capsule of compound oil of jujube, arboruitae, and gardenia. The normal control and model control groups were given the same amount of saline. The positive control group was given metformin hydrochloride and the intervention groups were given different doses of Soft capsule of compound oil of jujube,arboruitae,and gardenia. After 8 weeks of intervention, the levels of blood glucose, blood lipid, creatinine and urea nitrogen were measured.Results At the end of the intervention, the body weight of each intervention group was higher than that of model control group(P<0.05).There were no significant differences in body weight, blood glucose, HDL-C, LDL-C, TC, BUN between high dose group and positive control group(P>0.05), but there were significant differences between high dose group and model group(P<0.05). Conclusions soft capsule of compound oil of jujube,arboruitae,and gardenia could improve glucose metabolism and lipid metabolism disorders in diabetic nephropathy rats, and promote the recovery of renal function in diabetic rats.
引文
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2 Chen CJ,Liao WL,Chang CT,et al.Urine proteome analysis by C18 plate-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis and prediction of diabetic nephropathy[J].PLoS One,2018;13(7):1-14.
3 张丽芬,吕仁和,黄文政.链脲佐菌素糖尿病肾病大鼠模型的建立及稳定性评价[J].中国比较医学杂志,2014;24(4):8-12,18.
4 Gilbert RE,Cooper ME.The tubulointerstitium in progressive diabetic kidney disease:more than an aftermath of glomerular injury[J]?Kidney Int,1999;56(5):1627-37.
5 Kim SY,Jeong TD,Lee W,et al.Plasma neutrophil gelatinase-associated lipocalin as a marker of tubular damage in diabetic nephropathy[J].Anna Lab Med,2018;38(6):524-9.
6 李宝莉,谷秀娟,陈雅慧,等.酸柏栀油软胶囊对去势大鼠血液流变性的影响及其降血脂作用[J].吉林大学学报(医学版),2012;38(5):927-31.
7 张正祥,付佳廷,景东华,等.酸柏栀油软胶囊抗氧化作用初步研究[J].陕西医学杂志,2016;45(3):267-70.
8 陈永欣,韦锦斌.链脲佐菌素诱导大鼠2型糖尿病并发症模型研究进展[J].中国比较医学杂志,2013;23(3):63-6.
9 刘素芳,刘丽秋,杨鹏鹏.RANK-RANKL在2型糖尿病肾病大鼠肾脏表达的研究[J].中国实验诊断学,2018;22(4):695-9.
10 童东,王文倩,罗煜,等.藏药四味姜黄方对STZ诱导糖尿病肾病大鼠模型的剂量配比关系的初步研究[J].中成药,2018;40(3):516-24.
11 李宝莉,朱梅,陈雅慧,等.酸柏栀油软胶囊中脂肪酸和维生素E的组成[J].中国老年学杂志,2013;33(13):3106-7.
12 Oktem F,Ozguner F,Yilmaz HR,et al.Melatonin reduces urinary excretion of N-acetyl-beta-D-glucosaminidase,albumin and renaloxidative markers in diabetic rats[J].Clin Exp Pharmacol Physiol,2006;33(1-2):95-101.
13 Cam M,Yavuz O,Guven A,et al.Protective effects of chronic melatonin treatment against renal injury in streptozotocin-induceddiabetic rats[J].J Pineal Res,2003;35(3):212-20.
14 李军,韩玲,董力,等.2型糖尿病患者血清维生素E含量与胰岛素抵抗、氧化应激反应的相关性研究[J].海南医学院学报,2017;23(16):2200-2,2206.
15 孙永叶,戴云鹤,张华琦,等.维生素E对糖尿病胰岛素抵抗的影响及其相关机制分析[J].微量元素与健康研究,2015;32(5):53-5.
16 Farvid MS,Jalali M,Siassi F,et al.Comparison of the effects of vitamins and/or mineral supplementation on glomerular and tubular dysfunction in type 2 diabetes[J].Diabetes Care,2005;28(10):2458-64.
17 马琦,袁其朋,丁轲,等.酸枣仁抗氧化成分提取及其活性[J].过程工程学报,2010;10(4):756-61.
18 Fedor D,Kelley DS.Prevention of insulin resistance by n-3 polyunsaturated fatty acids[J].Curr Opin Clin Nutr Metab Care,2009;12(2):138-46.
19 Le Jossic-Corcos C,Gonthier C,Zaghini I,et al.Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids[J].Biochem J,2005;385(3):787-94.
20 李霞,袁凤来,袁丽萍,等.多不饱和脂肪酸调血脂作用研究进展[J].安徽医药,2007;11(10):867-9.
21 司芹芹,牛晓红,李俊岩,等.酸枣仁皂苷A对糖尿病模型大鼠肾小球细胞凋亡的影响[J].中国药理学与毒理学杂志,2017;31(5):399-404.
22 Cai M,Yu Z,Wang L,et al.Tongxinluo reduces brain edema and inhibits post-ischemic inflammation after middle cerebral artery occlusion in rats[J].J Ethnopharmacol,2016;181:136-45.
2 Chen CJ,Liao WL,Chang CT,et al.Urine proteome analysis by C18 plate-matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis and prediction of diabetic nephropathy[J].PLoS One,2018;13(7):1-14.
3 张丽芬,吕仁和,黄文政.链脲佐菌素糖尿病肾病大鼠模型的建立及稳定性评价[J].中国比较医学杂志,2014;24(4):8-12,18.
4 Gilbert RE,Cooper ME.The tubulointerstitium in progressive diabetic kidney disease:more than an aftermath of glomerular injury[J]?Kidney Int,1999;56(5):1627-37.
5 Kim SY,Jeong TD,Lee W,et al.Plasma neutrophil gelatinase-associated lipocalin as a marker of tubular damage in diabetic nephropathy[J].Anna Lab Med,2018;38(6):524-9.
6 李宝莉,谷秀娟,陈雅慧,等.酸柏栀油软胶囊对去势大鼠血液流变性的影响及其降血脂作用[J].吉林大学学报(医学版),2012;38(5):927-31.
7 张正祥,付佳廷,景东华,等.酸柏栀油软胶囊抗氧化作用初步研究[J].陕西医学杂志,2016;45(3):267-70.
8 陈永欣,韦锦斌.链脲佐菌素诱导大鼠2型糖尿病并发症模型研究进展[J].中国比较医学杂志,2013;23(3):63-6.
9 刘素芳,刘丽秋,杨鹏鹏.RANK-RANKL在2型糖尿病肾病大鼠肾脏表达的研究[J].中国实验诊断学,2018;22(4):695-9.
10 童东,王文倩,罗煜,等.藏药四味姜黄方对STZ诱导糖尿病肾病大鼠模型的剂量配比关系的初步研究[J].中成药,2018;40(3):516-24.
11 李宝莉,朱梅,陈雅慧,等.酸柏栀油软胶囊中脂肪酸和维生素E的组成[J].中国老年学杂志,2013;33(13):3106-7.
12 Oktem F,Ozguner F,Yilmaz HR,et al.Melatonin reduces urinary excretion of N-acetyl-beta-D-glucosaminidase,albumin and renaloxidative markers in diabetic rats[J].Clin Exp Pharmacol Physiol,2006;33(1-2):95-101.
13 Cam M,Yavuz O,Guven A,et al.Protective effects of chronic melatonin treatment against renal injury in streptozotocin-induceddiabetic rats[J].J Pineal Res,2003;35(3):212-20.
14 李军,韩玲,董力,等.2型糖尿病患者血清维生素E含量与胰岛素抵抗、氧化应激反应的相关性研究[J].海南医学院学报,2017;23(16):2200-2,2206.
15 孙永叶,戴云鹤,张华琦,等.维生素E对糖尿病胰岛素抵抗的影响及其相关机制分析[J].微量元素与健康研究,2015;32(5):53-5.
16 Farvid MS,Jalali M,Siassi F,et al.Comparison of the effects of vitamins and/or mineral supplementation on glomerular and tubular dysfunction in type 2 diabetes[J].Diabetes Care,2005;28(10):2458-64.
17 马琦,袁其朋,丁轲,等.酸枣仁抗氧化成分提取及其活性[J].过程工程学报,2010;10(4):756-61.
18 Fedor D,Kelley DS.Prevention of insulin resistance by n-3 polyunsaturated fatty acids[J].Curr Opin Clin Nutr Metab Care,2009;12(2):138-46.
19 Le Jossic-Corcos C,Gonthier C,Zaghini I,et al.Hepatic farnesyl diphosphate synthase expression is suppressed by polyunsaturated fatty acids[J].Biochem J,2005;385(3):787-94.
20 李霞,袁凤来,袁丽萍,等.多不饱和脂肪酸调血脂作用研究进展[J].安徽医药,2007;11(10):867-9.
21 司芹芹,牛晓红,李俊岩,等.酸枣仁皂苷A对糖尿病模型大鼠肾小球细胞凋亡的影响[J].中国药理学与毒理学杂志,2017;31(5):399-404.
22 Cai M,Yu Z,Wang L,et al.Tongxinluo reduces brain edema and inhibits post-ischemic inflammation after middle cerebral artery occlusion in rats[J].J Ethnopharmacol,2016;181:136-45.