乐昌含笑基因收集库中植株生长情况研究
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摘要
在前期开展乐昌含笑多点种源家系试验的基础上,通过嫁接方法建立基因收集库,收集保存乐昌含笑育种材料,调查分析基因收集库中植株生长情况。结果表明,嫁接成活率可达83.39%,能达到较好的建库效果;接穗径向粗度、接穗高度2个生长性状的统计分析表明,基因收集库中的基因型存在显著的遗传差异,显示出丰富的遗传多样性;盒状图分析结果表明,同一基因型中的不同嫁接株也存在一定的差异。基因收集库的建立对乐昌含笑今后的遗传改良研究有积极的作用。
Based on the early research of provenance and family trial of Michelia chapensis Dandy,grafting was adopted to establish a gene collection library for the preservation of genetic material of Michelia chapensis Dandy.Results obtained from growth analysis of plants in gene collection library indicated that the grafting survival ratio was as high as 83.39%.The difference of diameter and height of the grafting was reached high significant level,which showed abundant genetic diversity.Boxplot analysis also indicated different grafted plants with the same genotype appeared some differences.The establishment of gene collection library has positive role for genetic improvement of Michelia chapensis Dandy.
Based on the early research of provenance and family trial of Michelia chapensis Dandy,grafting was adopted to establish a gene collection library for the preservation of genetic material of Michelia chapensis Dandy.Results obtained from growth analysis of plants in gene collection library indicated that the grafting survival ratio was as high as 83.39%.The difference of diameter and height of the grafting was reached high significant level,which showed abundant genetic diversity.Boxplot analysis also indicated different grafted plants with the same genotype appeared some differences.The establishment of gene collection library has positive role for genetic improvement of Michelia chapensis Dandy.
引文
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[3]韦如萍,胡德活,张照勋,等.乐昌含笑果实特征[J].广东林业科技,2006,22(1):60-63.
[4]王润辉,张伟红,郑会全,等.乐昌含笑多点种源试验与优良种源选择研究[J].中南林业科技大学学报,2014(5):16-21.
[5]胡德活,韦如萍,张照勋,等.乐昌含笑种源与家系种子育苗试验[J].广东林业科技,2006,22(3):1-7.
[6]张伟红,王润辉,郑会全,等.乐昌含笑优树多点子代测定及优良家系选择研究[J].浙江农林大学学报,2015,32(5):763-769.
[7]张伟红,王润辉,郑会全,等.乐昌含笑种源地理变异研究[J].西南林业大学学报,2015,35(2):24-29.
[8]邹寿明,晏姝,王润辉,等.乐昌含笑家系试验初报[J].广东林业科技,2009,25(2):10-15.
[9]王润辉,胡德活,郑会全,等.杉木无性系生长和材性变异及多性状指数选择[J].林业科学,2012,48(3):45-50.
[10]王润辉,胡德活.SAS Proc Mixed在林木育种试验统计中应用简介[J].广东林业科技,2011,27(3):50-54.
[11]黄少伟,谢维辉.实用SAS编程与林业试验数据分析[M].广州:华南理工大学出版社,2001.
[12]丘作忠,王润辉,郑会全,等,乐昌含笑优树子代测定及选择[J].广东林业科技,2015,31(3):1-5.