HNSCC患者肿瘤组织Smad7蛋白、CXCR4蛋白的表达变化及其意义
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摘要
目的探讨头颈部鳞状细胞癌(HNSCC)组织中的Smad7蛋白、趋化因子4(CXCR4)蛋白的表达变化及其意义。方法选取我院2016年1月-2017年1月病理科收集的80例HNSCC组织(HNSCC组)、对应部位的正常头颈部组织80例(对照组),采用免疫组化染色检测两组标本中的Smad7蛋白、CXCR4蛋白阳性表达率,并分析二者与HNSCC组织中的病理学分化程度、TNM分期、淋巴结转移、病灶直径之间的关系。结果 HNSCC组标本中的Smad7蛋白、CXCR4蛋白阳性表达率分别为72.50%、68.75%,对照组的Smad7蛋白、CXCR4蛋白阳性表达率分别为7.50%、10.00%,差异具有统计学意义(P<0.05);低分化、TNM分期(Ⅲ期+Ⅳ期)、发生淋巴结转移的HNSCC组织中的Smad7蛋白、阳性率显著的高于高中分化、TNM分期(Ⅰ期+Ⅱ期)、未发生淋巴结转移的HNSCC组织(P<0.05);TNM分期(Ⅲ期+Ⅳ期)、发生淋巴结转移的HNSCC组织中的Smad7蛋白、阳性率显著的高于TNM分期(Ⅰ期+Ⅱ期)、未发生淋巴结转移的HNSCC组织(P<0.05)。结论 HNSCC组织中的Smad7蛋白、CXCR4蛋白的表达水平升高,并且与肿瘤的病理学特征具有一定的相关性。
Objective To investigate the expression and significance of Smad7 protein and chemokine 4(CXCR4) protein in tissues of head and neck squamous cell carcinoma(HNSCC). Methods Eighty HNSCC tissues(HNSCC group) and 80 normal head and neck tissues(control group) were collected from the pathology department of First Affiliated Hospital of Henan University of Science and Technology from 2016.01 to 2017.01. Immunohistochemical staining was used to detect the positive expression rates of Smad7 protein and CXCR4 protein in the two groups. The relationship between the two and pathological differentiation,TNM stage,lymph node metastasis and lesion diameter in HNSCC tissues was analyzed. Results The positive expression rates of Smad7 protein and CXCR4 protein in HNSCC group were 72.50% and 68.75%,respectively. The positive expression rates of Smad7 protein and CXCR4 protein in the control group were 7.50% and 10.00%,respectively(P<0.05). The positive rate of Smad7 protein in HNSCC tissues with poor differentiation,TNM stage(stage Ⅲ+Ⅳ) and lymph node metastasis was significantly higher than that of high-medium differentiation,TNM stage(stage Ⅰ+Ⅱ),and HNSCC tissues without lymph node metastasis(P<0.05);The positive rate of Smad7 protein in TNS stage(stage Ⅲ+Ⅳ) and HNSCC with lymph node metastasis was significantly higher than that in TNM stage(stage Ⅰ+Ⅱ) and HNSCC without lymph node metastasis(P<0.05). Conclusion The expression levels of Smad7 protein and CXCR4 protein in HNSCC tissues are elevated and have a certain correlation with the pathological features of tumors.
Objective To investigate the expression and significance of Smad7 protein and chemokine 4(CXCR4) protein in tissues of head and neck squamous cell carcinoma(HNSCC). Methods Eighty HNSCC tissues(HNSCC group) and 80 normal head and neck tissues(control group) were collected from the pathology department of First Affiliated Hospital of Henan University of Science and Technology from 2016.01 to 2017.01. Immunohistochemical staining was used to detect the positive expression rates of Smad7 protein and CXCR4 protein in the two groups. The relationship between the two and pathological differentiation,TNM stage,lymph node metastasis and lesion diameter in HNSCC tissues was analyzed. Results The positive expression rates of Smad7 protein and CXCR4 protein in HNSCC group were 72.50% and 68.75%,respectively. The positive expression rates of Smad7 protein and CXCR4 protein in the control group were 7.50% and 10.00%,respectively(P<0.05). The positive rate of Smad7 protein in HNSCC tissues with poor differentiation,TNM stage(stage Ⅲ+Ⅳ) and lymph node metastasis was significantly higher than that of high-medium differentiation,TNM stage(stage Ⅰ+Ⅱ),and HNSCC tissues without lymph node metastasis(P<0.05);The positive rate of Smad7 protein in TNS stage(stage Ⅲ+Ⅳ) and HNSCC with lymph node metastasis was significantly higher than that in TNM stage(stage Ⅰ+Ⅱ) and HNSCC without lymph node metastasis(P<0.05). Conclusion The expression levels of Smad7 protein and CXCR4 protein in HNSCC tissues are elevated and have a certain correlation with the pathological features of tumors.
引文
[1]Sandu K,Nisa L,Monnier P,et al.Clinicobiological progression and prognosis of oral squamous cell carcinoma in relation to the tumor invasive front:impact on prognosis[J].Acta Otolaryngol,2014,134(4):416-424.
[2]Luo S,Chen Y,Liu C,et al.Osteopontin Involves Cisplatin Resistance and Poor Prognosis in Oral Squamous Cell Carcinoma[J].Biomed Res Int,2015,25(6):1-13.
[3]李文友,宋丽芳,李天翔,等.TGF-β1、Smad4、Smad7在口腔鳞癌组织中的表达及临床意义[J].国际医药卫生导报,2017,23(3):322-325.
[4]尹东,金立元,张佐,等.趋化因子SDF-1及其受体CXCR4对口腔鳞癌细胞生物学行为的影响[J].北京口腔医学,2015,23(2):85-88.
[5]尹东,张娜,封净,等.SDF-1/CXCR4生物轴对口腔鳞癌细胞增殖与侵袭的影响[J].宁夏医学杂志,2017,39(8):812-814.
[6]Shinriki S,Jono H,Ueda M,et al.Stromal expression of neutrophil gelatinase-associated lipocalin correlates with poor differentiation and adverse prognosis in oral squamous cell carcinoma[J].Histopathology,2014,64(3):356-364.
[7]Cheng M,Huang M,Lin C,et al.Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma[J].Histopathology,2014,65(1):24-34.
[8]Hutchison I.Improving the prognosis of oral squamous cell carcinoma in 2013[J].Br Dent J,2013,215(9):441-443.
[9]Yoshida R,Nakayama H,Nagata M,et al.Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma[J].Br J Cancer,2014,111(12):2308-2315.
[10]刘一秀,夏德林.趋化因子受体CXCR4及其配体CXCL12与口腔癌研究进展[J].西南军医,2014,16(3):278-280.
[11]尹东,严伟,张佐,等.抗CXCR4单克隆抗体对口腔鳞癌细胞增殖与侵袭的影响[J].宁夏医学杂志,2016,38(2):117-119.
[12]刘进忠,李媛,周建炜,等.Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义[J].上海口腔医学,2017,26(4):409-413.
[13]郑琛,石超吉,郑波,等.Smad7蛋白在口腔鳞癌组织中的表达及其对口腔鳞癌细胞增殖和迁移能力的影响[J].中国口腔颌面外科杂志,2017,15(3):220-226.
[14]张舒,王博,周旋,等.趋化因子CXC亚家族受体4调控口腔鳞状细胞癌上皮间质转化介导淋巴转移的体外实验[J].中华口腔医学杂志,2014,34(3):171-176.
[15]宋国华,李莉红,皇甫冰,等.EGFR,PDCD4,TGF-β1,Smad3,Smad7在中国地鼠口腔颊囊黏膜癌变过程中的表达研究[J].中国比较医学杂志,2017,27(5):64-70.
[16]傅新文,胡银英,宋晓萍,等.头颈部肿瘤多瘤抑制基因p16表达研究[J].实验与检验医学,2010,28(5):439-440.
[2]Luo S,Chen Y,Liu C,et al.Osteopontin Involves Cisplatin Resistance and Poor Prognosis in Oral Squamous Cell Carcinoma[J].Biomed Res Int,2015,25(6):1-13.
[3]李文友,宋丽芳,李天翔,等.TGF-β1、Smad4、Smad7在口腔鳞癌组织中的表达及临床意义[J].国际医药卫生导报,2017,23(3):322-325.
[4]尹东,金立元,张佐,等.趋化因子SDF-1及其受体CXCR4对口腔鳞癌细胞生物学行为的影响[J].北京口腔医学,2015,23(2):85-88.
[5]尹东,张娜,封净,等.SDF-1/CXCR4生物轴对口腔鳞癌细胞增殖与侵袭的影响[J].宁夏医学杂志,2017,39(8):812-814.
[6]Shinriki S,Jono H,Ueda M,et al.Stromal expression of neutrophil gelatinase-associated lipocalin correlates with poor differentiation and adverse prognosis in oral squamous cell carcinoma[J].Histopathology,2014,64(3):356-364.
[7]Cheng M,Huang M,Lin C,et al.Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma[J].Histopathology,2014,65(1):24-34.
[8]Hutchison I.Improving the prognosis of oral squamous cell carcinoma in 2013[J].Br Dent J,2013,215(9):441-443.
[9]Yoshida R,Nakayama H,Nagata M,et al.Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma[J].Br J Cancer,2014,111(12):2308-2315.
[10]刘一秀,夏德林.趋化因子受体CXCR4及其配体CXCL12与口腔癌研究进展[J].西南军医,2014,16(3):278-280.
[11]尹东,严伟,张佐,等.抗CXCR4单克隆抗体对口腔鳞癌细胞增殖与侵袭的影响[J].宁夏医学杂志,2016,38(2):117-119.
[12]刘进忠,李媛,周建炜,等.Smad4、Smad7和Caveolin-1在Wistar大鼠口腔黏膜癌变中的表达和意义[J].上海口腔医学,2017,26(4):409-413.
[13]郑琛,石超吉,郑波,等.Smad7蛋白在口腔鳞癌组织中的表达及其对口腔鳞癌细胞增殖和迁移能力的影响[J].中国口腔颌面外科杂志,2017,15(3):220-226.
[14]张舒,王博,周旋,等.趋化因子CXC亚家族受体4调控口腔鳞状细胞癌上皮间质转化介导淋巴转移的体外实验[J].中华口腔医学杂志,2014,34(3):171-176.
[15]宋国华,李莉红,皇甫冰,等.EGFR,PDCD4,TGF-β1,Smad3,Smad7在中国地鼠口腔颊囊黏膜癌变过程中的表达研究[J].中国比较医学杂志,2017,27(5):64-70.
[16]傅新文,胡银英,宋晓萍,等.头颈部肿瘤多瘤抑制基因p16表达研究[J].实验与检验医学,2010,28(5):439-440.