非小细胞肺癌患者血和呼出气冷凝液转移生长因子β1的检测及诊断价值
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摘要
目的探讨非小细胞肺癌(NSCLC)患者血和呼出气冷凝液(EBC)中转移生长因子β1(TGF-β1)检测价值。方法收集NSCLC患者52例,其中鳞癌24例,腺癌28例,TNM分期的Ⅰ期～Ⅱ期18例,Ⅲ期～Ⅳ期34例,同期健康体检者35例为对照组,采集空腹血清和EBC标本,采用ELISA方法检测TGF-β1。结果 NSCLC组血清、EBC中TGF-β1水平均高于对照组,差异有统计学意义(P <0. 01)。腺癌组血清和EBC中TGF-β1水平均高于鳞癌组,差异有统计学意义(P <0. 01)。Ⅲ期～Ⅳ期血清和EBC中TGF-β1均高于Ⅰ期～Ⅱ期,差异有统计学意义(P <0. 01);Ⅰ期～Ⅱ期血清和EBC中TGF-β1水平均高于对照组,差异有统计学意义(P <0. 01)。结论检测血及EBC中TGF-β1水平对肺癌有辅助诊断价值,有助于病理分型,可作为预后参考指标。
Objective To observe the diagnostic value of transforming growth factor β1( TGF-β1) in serum and exhaled breath condensate( EBC) in non-small cell lung cancer( NSCLC). Methods Totally 52 NSCLC patients were collected,including 24 cases of squamous carcinoma,28 cases of adenocarcinoma,and 18 cases in stage Ⅰ-Ⅱ,34 cases in stageⅢ-Ⅳ. 35 healthy subjects were enrolled in the control group. Serum and EBC specimens were collected and enzyme-linked immunosorbent assay( ELISA) was used for the detection of TGF-β1. Results In the NSCLC group,TGF-β1 levels in serum and EBC were significantly higher than those in control group,with the difference statistically significant( P < 0. 01).The TGF-β1 levels in serum and EBC in adenocarcinoma group were significantly higher than squamous cell carcinoma group,with the difference statistically significant( P < 0. 01). According to the TNM stage,TGF-β1 levels in serum and EBC in NSCLC Ⅲ-Ⅳ stages were significantly higher than stages Ⅰ-Ⅱ,with the difference statistically significant( P < 0. 01); and TGF-β1 levels in serum and EBC in stages Ⅰ-Ⅱ were significantly higher than control group,with the difference statistically significant( P < 0. 01). Conclusion The detection of TGF-β1 levels in serum and EBC have assistant diagnostic value,which is helpful for pathological types and can be used as a prognostic index.
Objective To observe the diagnostic value of transforming growth factor β1( TGF-β1) in serum and exhaled breath condensate( EBC) in non-small cell lung cancer( NSCLC). Methods Totally 52 NSCLC patients were collected,including 24 cases of squamous carcinoma,28 cases of adenocarcinoma,and 18 cases in stage Ⅰ-Ⅱ,34 cases in stageⅢ-Ⅳ. 35 healthy subjects were enrolled in the control group. Serum and EBC specimens were collected and enzyme-linked immunosorbent assay( ELISA) was used for the detection of TGF-β1. Results In the NSCLC group,TGF-β1 levels in serum and EBC were significantly higher than those in control group,with the difference statistically significant( P < 0. 01).The TGF-β1 levels in serum and EBC in adenocarcinoma group were significantly higher than squamous cell carcinoma group,with the difference statistically significant( P < 0. 01). According to the TNM stage,TGF-β1 levels in serum and EBC in NSCLC Ⅲ-Ⅳ stages were significantly higher than stages Ⅰ-Ⅱ,with the difference statistically significant( P < 0. 01); and TGF-β1 levels in serum and EBC in stages Ⅰ-Ⅱ were significantly higher than control group,with the difference statistically significant( P < 0. 01). Conclusion The detection of TGF-β1 levels in serum and EBC have assistant diagnostic value,which is helpful for pathological types and can be used as a prognostic index.
引文
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[11] Chen KC,Chen CY,Lin CR,et al. Luteolin attenuates TGF-β1-induced epithelial-mesenchymal transition of lung cancer cells by interfering in the PI3K/Akt-NF-κB-Snail pathway[J]. Life Sci,2013,93(24):924-933.
[12] Li H,Da LJ,Fan WD,et al. Transcription factor glioma-associated oncogene homolog 1 is required for transforming growth factor-β1-induced epithelial-mesenchymal transition of non-small cell lung cancer cells[J]. Mol Med Rep,2015,11(5):3259-3268.
[13] Huang AL,Liu SG,Qi WJ,et al. TGF-β1 protein expression in non-small cell lung cancers is correlated with prognosis[J]. Asian Pac J Cancer Prev,2014,15(19):8143-8147.
[14] Liu RY,Zeng Y,Lei Z,et al. JAK/STAT3 signaling is required for TGF-β-induced epithelial-mesenchymal transition in lung cancer cells[J]. Int J Oncol,2014,44(5):1643-1651.
[15] Lei Z,Xu G,Wang L,et al. MiR-142-3p represses TGF-β-induced growth inhibition through repression of TGFβR1 in nonsmall cell lung cancer[J]. FASEB J,2014,28(6):2696-2704.
[2]孙健,沈巨信,周国忠,等. COPD患者呼出气冷凝液中8-异前列腺素及白细胞介素6水平的差异及临床意义[J].中国呼吸与危重监护杂志,2010,9(2):137-140.
[3]沈巨信,秦娥,李明晖,等.呼出气冷凝液白三烯、8异前列腺素、硝酸盐/亚硝酸盐检测在哮喘中的应用及孟鲁司特钠对炎症指标的影响[J].中国临床药理学与治疗学,2012,17(7):802-805.
[4]秦娥,沈巨信,周国忠,等.哮喘患者呼出气冷凝液白三烯检测及孟鲁司特的抗炎作用[J].中国卫生检验杂志,2013,23(2):390-394.
[5]秦娥,沈巨信,周国忠.非小细胞肺癌患者呼出气冷凝液中不均一核糖核蛋白A2/B1检测的临床意义[J].中国卫生检验杂志,2016,26(22):3250-3254.
[6]乔华,王广发,张红,等.阻塞性睡眠呼吸暂停低通气综合征患者呼出气冷凝液中白细胞介素6的研究[J].中华结核和呼吸杂志,2005,28(6):364-367.
[7]王怡,刘沙,魏溪,等. IL-29和TGF-β1在肺癌中的表达及临床意义[J].江苏大学学报:医学版,2016,26(5):458-460.
[8] Hou YL,Chen H,Dong ZH,et al. Clinical significance of serum transforming growth factor-β1 in lung cancer[J]. Cancer Epidemiol,2013,37(5):750-753.
[9] Domagala-Kulawik J,Hoser G,Safianowska A,et al. Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer[J]. Arch Immunol Ther Exp,2006,54(2):143-147.
[10] Ansieau S,Collin G,Hill L. EMT or EMT-promoting transcription factors,where to focus the light?[J]. Front Oncol,2014,4:353.
[11] Chen KC,Chen CY,Lin CR,et al. Luteolin attenuates TGF-β1-induced epithelial-mesenchymal transition of lung cancer cells by interfering in the PI3K/Akt-NF-κB-Snail pathway[J]. Life Sci,2013,93(24):924-933.
[12] Li H,Da LJ,Fan WD,et al. Transcription factor glioma-associated oncogene homolog 1 is required for transforming growth factor-β1-induced epithelial-mesenchymal transition of non-small cell lung cancer cells[J]. Mol Med Rep,2015,11(5):3259-3268.
[13] Huang AL,Liu SG,Qi WJ,et al. TGF-β1 protein expression in non-small cell lung cancers is correlated with prognosis[J]. Asian Pac J Cancer Prev,2014,15(19):8143-8147.
[14] Liu RY,Zeng Y,Lei Z,et al. JAK/STAT3 signaling is required for TGF-β-induced epithelial-mesenchymal transition in lung cancer cells[J]. Int J Oncol,2014,44(5):1643-1651.
[15] Lei Z,Xu G,Wang L,et al. MiR-142-3p represses TGF-β-induced growth inhibition through repression of TGFβR1 in nonsmall cell lung cancer[J]. FASEB J,2014,28(6):2696-2704.