秦岭龙胆挥发油成分分析及抗氧化活性研究
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摘要
目的对比秦岭龙胆挥发油酯化前后化学成分及相对含量的变化,并评价其抗氧化能力。方法利用超声辅助提取技术提取秦岭龙胆中的挥发油,并对挥发油进行甲酯化。利用气相色谱-质谱联用技术(GC-MS)对酯化前后的化学成分进行分析;利用清除DPPH自由基及ABTS自由基测定秦岭龙胆挥发油的抗氧化活性。结果GC-MS结果显示,秦岭龙胆挥发性成分组成在酯化前后发生了较大变化,酯化前共分析出26种成分,酯化后分析出25种成分,其中有相同成分16种;其主要成分含量在酯化前后亦发生了较大变化,如不饱和脂肪酸亚油酸从酯化前的1.56%上升到酯化后的6.72%,油酸从酯化前的0.42%上升到4.92%;秦岭龙胆总挥发油有一定的抗氧化活性,对DPPH及ABTS自由基阳离子均有一定的清除作用,其中对DPPH的清除率远大于标准品抗氧化剂VC。结论秦岭龙胆挥发性成分组成在酯化前后组成及含量均发生了较大变化,且秦岭龙胆挥发油成分有一定的抗氧化活性。
Objective To study the change of chemical composition and relative content of volatile oil before andafter esterification in volatile oil from Gentiana apiata N. E. Br.,and evaluate its antioxidant activity. Method Thevolatile oil from Gentiana apiata was extracted by ultrasonic assisted extraction, and the volatile oil was methylesterified. The change of its components before and after esterification was examined by GC-MS. Antioxidant activitywas assessed by scavenging rate of DPPH and ABTS+·. Results The volatile oil composition changed greatly beforeand after esterification. A total of 26 components were detected before esterification, and 25 components weredetected after esterification, of which 16 were the same components. The main components' contents were alsogreatly changed,for example,unsaturated fatty acid linoleic acid increased from 1.56 % to 6.72 %,and oleic acidincreased from 0.42 % to 4.92 %. The volatile oil from Gentiana apiata could scavenge DPPH and ABTS+·,and theremoval rate of DPPH is much greater than the standard antioxidant VC. Conclusion The composition and contentof the volatile oil from Gentiana apiata changed greatly before and after esterification, and the volatile oil from Gentiana apiata showed certain antioxidant activity.
Objective To study the change of chemical composition and relative content of volatile oil before andafter esterification in volatile oil from Gentiana apiata N. E. Br.,and evaluate its antioxidant activity. Method Thevolatile oil from Gentiana apiata was extracted by ultrasonic assisted extraction, and the volatile oil was methylesterified. The change of its components before and after esterification was examined by GC-MS. Antioxidant activitywas assessed by scavenging rate of DPPH and ABTS+·. Results The volatile oil composition changed greatly beforeand after esterification. A total of 26 components were detected before esterification, and 25 components weredetected after esterification, of which 16 were the same components. The main components' contents were alsogreatly changed,for example,unsaturated fatty acid linoleic acid increased from 1.56 % to 6.72 %,and oleic acidincreased from 0.42 % to 4.92 %. The volatile oil from Gentiana apiata could scavenge DPPH and ABTS+·,and theremoval rate of DPPH is much greater than the standard antioxidant VC. Conclusion The composition and contentof the volatile oil from Gentiana apiata changed greatly before and after esterification, and the volatile oil from Gentiana apiata showed certain antioxidant activity.
引文
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[2]许定发.中国经济植物:上卷[M].江苏:江苏凤凰科学技术出版社,2015:730.
[3]许海燕,郑伶俐,多本加,等.响应面法优化秦岭龙胆有效成分的提取工艺[J].中国实验方剂学杂志,2014,20(15):31-34.
[4]孙汉巨.食品分析与检测[M].合肥:合肥工业大学出版社,2016:178.
[5]赵维波,张丹雁,徐展翅,等.斜叶黄檀香材挥发油成分及抗氧化活性研究[J].中药新药与临床药理,2017,28(5):659-662.
[6]陈丽萍,王先敏,李茂星,等.栀子中抗氧化的活性成分研究[J].华西药学杂志,2018,33(2):179-182.
[7]吴世敏,印德麟.简明精细化工大辞典[M].沈阳:辽宁科学技术出版社,1999:361.
[8]陈叶,石秀云,李海亮,等.窄叶鲜卑花果穗挥发油成分分析[J].中药材,2016,39(10):2261-2263.
[9]惠阳,刘园,林婧,等.三叶鬼针草不同部位挥发油成分的GC-MS分析[J].化学研究与应用,2017,29(1):19-24.
[10]ZELLER MH,INGE T H,MODI A C,et al.Severe obesity and comorbid condition impact on the weight-related quality of life of the adolescent patients[J].Journal of pediatric,2015,166(3):651-659.
[11]Bolsoni-Lopes A,Festuccia W T,Chimin P,et al.Palmitoleic acid(n-7)increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation[J].Lipids in Health and Disease,2014,13:199.
[12]YANH Z H,TAKEO J,KATAYAMA M.Oral administration of omega-7 palmitoleic acid induces satiety and the release of appetiterelated hormones in male rats[J].Appetite,2013,65:1-7.