响应曲面法优化超声-微波辅助提取秦岭龙胆挥发油的工艺研究
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摘要
利用响应曲面法(RSM)优化超声-微波辅助提取秦岭龙胆挥发油的工艺条件。在单因素实验基础上,选取超声功率、微波功率、提取时间、液固比为自变量,以乙醚为浸提溶剂,挥发油得率为响应值,应用中心组合实验设计方法,研究各自变量及其交互作用对挥发油得率的影响,建立二次多项回归方程预测数学模型。结果表明,超声功率、微波功率和提取时间3个因素对挥发油提取率有显著影响。确定超声-微波辅助提取秦岭龙胆挥发油的最佳工艺参数超声功率270 W,微波功率570 W,液固比25 mL/g,提取时间11 min,在此条件下,挥发油的提取率达到了7.183%。
Through response surface methodology( RSM),ultrasonic-microwave synergistic extraction processing of volatile oil from Gentiana apiata was optimized. By using diethyl ether as the solvent,and based on the single factor analysis,the effect of ultrasonic power,microwave power,extraction time and solid-to-liquid ratio on volatile oil yield from Gentiana apiata was analyzed. The interactions of each factor on the oil yield were also investigated by application of central composite experimental design. The results showed that the ultrasonic power,microwave power and extraction time had significant effects on extraction yield. The optimal conditions of oil extraction from Gentiana apiata by the ultrasonic-microwave synergistic were ultrasonic power 270 W,microwave power 570W,liquid /solid 25 mL /g,and extraction time 11 min. Under such conditions,the oil yield was 7. 183 %.
Through response surface methodology( RSM),ultrasonic-microwave synergistic extraction processing of volatile oil from Gentiana apiata was optimized. By using diethyl ether as the solvent,and based on the single factor analysis,the effect of ultrasonic power,microwave power,extraction time and solid-to-liquid ratio on volatile oil yield from Gentiana apiata was analyzed. The interactions of each factor on the oil yield were also investigated by application of central composite experimental design. The results showed that the ultrasonic power,microwave power and extraction time had significant effects on extraction yield. The optimal conditions of oil extraction from Gentiana apiata by the ultrasonic-microwave synergistic were ultrasonic power 270 W,microwave power 570W,liquid /solid 25 mL /g,and extraction time 11 min. Under such conditions,the oil yield was 7. 183 %.
引文
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[2]吴振海,傅青,陈书文,等.陕西特有的中草药[J].中国药学杂志,1991,26(11):654.
[3]许海燕.秦岭龙胆化学成分的研究进展[J].亚太传统医药,2010,6(6):168-169.
[4]温悦,宋洁.挥发油提取分离研究概况[J].中国药业,2011,20(5):78-80.
[5]Wang Q,Jiang L,Wen QB.Effect of three extraction methods on the volatile component of Illicium verum Hook.fanalyzed by GC-MS[J].Wuhan University Journal of Natural Sciences,2007,12(3):529-534.
[6]左笑,张东翔.超声波在油脂提取中的应用[J].粮油加工,2007(11):70-72.
[7]张晓燕,丘泰球,徐彦渊,等.双频超声强化提取葛根有效部位的研究[J].食品工业科技,2006,27(3):51-52.
[8]Zhang S Q,Chen R Z,Wang C Z.Experiment study on ultrahigh pressure extraction of ginsenosides[J].Journal of Food Engineering,2007,79(1):1-5.
[9]ZHANG Zhenshan,WANG Lijun,LI Dong,et al.Ultrasound-assisted extraction of oil from flaxseed[J].Separation and Purification Technology,2008,62(1):192-198.
[10]陈丛瑾,黎跃,李欣.植物挥发油的提取技术研究进展[J].食品研究与开发,2011,32(11):151-156.
[11]Cheng X L,Wan J Y,Li P,et al.Ultrasonic/microwave assisted extraction and diagnosticion filtering strategy by liquid chromatography-quadrupole time-of-flight mas spectrometry for rapid characterization of flavonoids in spatholobus suberectus[J].Journal of Chromatogr A,2011,1218:5774-5786.
[12]徐秀泉,孙雪琴,邱晶波,等.响应面分析法优化生姜挥发油提取工艺[J].应用化工,2011,40(6):1035-1038.
[13]秦娇,姚永红,侯智霞,等.响应面分析法优化毛竹叶挥发油提取工艺[J].食品科学,2010,31(6):1-5.
[14]张薇,魏翔,谢世清,等.响应面法优化草果挥发油提取工艺条件的筛选研究[J].西部林业科学,2013,42(3):28-33.
[15]沈小钟,杨帆,于荣敏.响应面法优选黄芪提取工艺[J].中国实验方剂学杂志,2012,18(18):37-41.