微波与超声-微波在秦岭龙胆挥发油分析中的对比研究
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摘要
目的研究秦岭龙胆用不同提取方法提取挥发油成分的异同。方法采用微波辅助提取法(MA)、超声-微波双辅助(UME)提取秦岭龙胆中的挥发油。采用GC-MS结合计算机检索对所得挥发油成分进行分析和鉴定,峰面积归一法测定各组分的相对含量。结果结果表明,2种方法分别从秦岭龙胆所得挥发油中鉴定出24及29种化合物。结论不同方法所得秦岭龙胆挥发油化学组成各有异同,但主要成分是2,2’-亚甲基双(四-甲基-6-叔丁基)-苯酚、棕榈酸、油酸、亚油酸等。
Objective To study differences of chemical constituents between the two kinds of essential oils from Gentiana apiata N. E. Br. Methods Gentiana apiata N. E. Br. is a popular medicinal plant traditionally used in China to treat several diseases.In order to enlarge the medication scope,the constituents of essential oils which were extracted by means of microwave-assisted(MA) and ultrasonic-microwave synergistic extraction(UAMA) from Gentiana apiata N. E. Br. were analyzed and identifi ed with GC-MS. Results Based on the standard mass spectral data,24 and 29 kinds of volatile components were identifi ed respectively.Relative mass fractions of the constituents were determined by area normalization method.Conclusion There were differences in some chemical constituents among the two kinds of essential oils.But their main constituents were Oleic acid,Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl), linoleic acid, palmitic acid, stearic acid and so on.
Objective To study differences of chemical constituents between the two kinds of essential oils from Gentiana apiata N. E. Br. Methods Gentiana apiata N. E. Br. is a popular medicinal plant traditionally used in China to treat several diseases.In order to enlarge the medication scope,the constituents of essential oils which were extracted by means of microwave-assisted(MA) and ultrasonic-microwave synergistic extraction(UAMA) from Gentiana apiata N. E. Br. were analyzed and identifi ed with GC-MS. Results Based on the standard mass spectral data,24 and 29 kinds of volatile components were identifi ed respectively.Relative mass fractions of the constituents were determined by area normalization method.Conclusion There were differences in some chemical constituents among the two kinds of essential oils.But their main constituents were Oleic acid,Phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl), linoleic acid, palmitic acid, stearic acid and so on.
引文
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[2]许海燕.秦岭龙胆中齐墩果酸的提取工艺研究[J].现代中药研究与实践,2009,(5):68-69.
[3]张丽媛,申书昌,刘志明,等.微波法辅助萃取万寿菊花挥发油及其化学成分的气质联用分析[J].食品科学,2011,32(16):326-330.
[4]刘瑞林,王德金,袁静,等.超声#微波协同提取山茱英籽油工艺优化研究[J].中国食品添加剂,2012,(5):83-91.
[5]汪洪武,刘艳清,韦寿莲,等.微波、超声波和水蒸气蒸馏在紫苏籽挥发油分析中的对比研究[J].精细化工,2011,28(6):544-548.
[6]曹雨虹,顾健,谭睿,等.钻叶龙胆挥发油化学成分的GC-MS分析[J].中国实验方剂学杂志,2013,19(5):91-93.
[7]李勇慧,曹晓燕,押辉远.大叶秦艽中脂肪酸及挥发油成分的GC-MS分析[J].中药材,2011,34(4):559-562.
[8]杨红澎,赵钰玲,蒋与刚,等.蓝玉簪龙胆挥发油化学成分气相色谱-质谱联用分析[J].时珍国医国药,2009,20(2):347-348.
[9]高咏莉,余振喜,林瑞超,等.藏药萝卜秦艽挥发油成分的GC-MS分析研究[J].中国现代药物应用,2009,3(4):25-261
[10]丛蒲珠,李笋玉.天然有机质谱学[M].北京:中国医药科技出版社,2003:23.
[11]Stenha E,Abrahamssen S,Mc Laferty F W.Registry of Mass Spectra Data[M].Base Vol1~2.Washington:V S Government Printing Office,1978:52.
[12]王积涛,张宝申,王永梅,等.有机化学[M].天津:南开大学出版社,2003:420.