羊蹄甲茎皮提取物抑菌活性及化学成分研究
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摘要
[目的]研究羊蹄甲茎皮抑菌活性成分,为其在植物源农药方面的应用提供理论依据。[方法]采用菌丝生长速度法测定抑菌活性,采用常规柱层析及制备型高效液相色谱法等手段分离活性成分。[结果]羊蹄甲茎皮乙酸乙酯部抑菌效果最佳。从该部位中分离得到6个单体化合物,其中化合物2~5为首次从羊蹄甲中分离得到。在20 mg/L下,地芰普内酯(3)、7S,8R,8R'-(-)5-methoxylariciresinol 4'-O-β-D-glucopyranoside(4)及槲皮素(6)对6种供试真菌的抑制率在32.08%~62.53%之间。生物显微镜观察结果显示:与对照组相比,处理组菌丝出现局部膨胀、排列杂乱无序、断裂等现象。[结论]羊蹄甲茎皮乙酸乙酯具有较好抑菌活性,所分离的化合物3、4和6为羊蹄甲中主要抑菌活性成分。
[Aims] The paper aims to study the antifungal components of Bauhinia purpurea Bark and to provide basis for the development in botanical pesticide field. [Methods] Growth rate method was used to determine the antifungal activity. Active constituents were separated by common column chromatography and preparative high performance liquid chromatography(PHPLC). [Results] The ethyl acetate extract showed the best activity against pathogenic fungis.Six compounds were isolated from the ethyl acetate extract, the compounds 2-5 were for the first time isolated from B.purpurea. At 20 mg/L, compounds Loliolide(3), 7 S, 8 R, 8 R′-(-)5-methoxylariciresinol 4'-O-β-D-glucopyranoside(4)and quercetin(6) exhibited significant inhibiting effect against 6 pathogenic fungis, with inhibitory rate between 32.08-62.53%. The shapes of pathogenic fungis were characterized by microscope, which showed the mycelias of treatment groups swelled, disorderly arranged and fractured compared to control groups. [Conclusions] The ethyl acetate extract of B. purpurea Bark have the best antibacterial activity, the compounds 3, 4 and 6 are the main antifungal components in B. purpurea.
[Aims] The paper aims to study the antifungal components of Bauhinia purpurea Bark and to provide basis for the development in botanical pesticide field. [Methods] Growth rate method was used to determine the antifungal activity. Active constituents were separated by common column chromatography and preparative high performance liquid chromatography(PHPLC). [Results] The ethyl acetate extract showed the best activity against pathogenic fungis.Six compounds were isolated from the ethyl acetate extract, the compounds 2-5 were for the first time isolated from B.purpurea. At 20 mg/L, compounds Loliolide(3), 7 S, 8 R, 8 R′-(-)5-methoxylariciresinol 4'-O-β-D-glucopyranoside(4)and quercetin(6) exhibited significant inhibiting effect against 6 pathogenic fungis, with inhibitory rate between 32.08-62.53%. The shapes of pathogenic fungis were characterized by microscope, which showed the mycelias of treatment groups swelled, disorderly arranged and fractured compared to control groups. [Conclusions] The ethyl acetate extract of B. purpurea Bark have the best antibacterial activity, the compounds 3, 4 and 6 are the main antifungal components in B. purpurea.
引文
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[2]吴贻谷,宋立人,胡烈,等.中华本草:第4卷[M].上海:上海科学技术出版社,1999:2982-3001.
[3]JYOTHI K S,SESHAGIRI M.In-vitro Activity of Saponins of Bauhinia purpurea,Madhuca longifolia,Celastrus paniculatus and Semecarpus anacardium on Selected Oral Pathogens[J].Journal of Dentistry,2012,9(4):216-224.
[4]ANNEGOWDA H V,MORDI M N,RAMANATHAN S,et al.Effect of Extraction Techniques on Phenolic Content,Antioxidant and Antimicrobial Activity of Bauhinia purpurea:HPTLC Determination of Antioxidants[J].Food Anal Methods,2012,5:226-233.
[5]MUTHU S,PERUMAL P.Isolation,Purification and Characterization of Antimicrobial Protein from Seedlings of Bauhinia purpurea L.[J].International Journal of Biological Macromolecules,2016,86:390-401.
[6]CHUAH E L,ZAKARIA Z A,SUHAILI Z,et al.Antimicrobial Activities of Plant Extracts against Methicillin-susceptible and Methicillin-resistant Staphylococcus aureus[J].Journal of Microbiology Research,2014,4(1):6-13.
[7]BHAWNA S N,BHARTI P D,AGARWAL Y K.Evaluation of Antimicrobial Activity of Bauhinia purpurea Leaves Under in vitro Conditions[J].Indian J Microbiol,2012,52(3):360-365.
[8]方中达.植病研究方法[M].北京:中国农业出版社,1998.
[9]HIROE K,SANAE H,YAYOI K,et al.Antioxidative Phenylpropanoids from Berries of Pimenta dioca[J].Phytochemistry,1993,52:1307-1312.
[10]LUISELLA V,MARIO D A,ANDREA G,et al.In vitro Antiplasmodial Activity of Extracts of Tristaniopsis Species and Identification of the Active Constituents:Ellagic Acid and3,4,5-Trimethoxyphenyl-(6'-O-galloyl)-O-β-D-glucopyranoside[J].Journal of Natural Products,2001,64:603-607.
[11]马河,李方丽,王芳,等.白花蛇舌草化学成分研究[J].中药材,2016,39(1):98-102.
[12]黄文武,孔德云,杨培明.小木通木脂素成分研究[J].中国天然药物,2003,1(4):199-202.
[13]郭妍,陈晨,高纯,等.地胆草的化学成分研究[J].天然产物研究与开发,2016,28:1051-1054.
[14]海力茜·陶尔大洪,徐建军,凯赛尔·阿不都克热木,等.新疆红芪的化学成分[J].华西药学杂志,2006,21(1):47-48.
[15]NI Lin,ZHOU Xing,MA Jie,et al.Wilfordonols A-D:Four New Norsesquiterpenes from The Leaves of Tripterygium wilfordii[J].Journal of Asian Natural Products Research,2015,17(6):615-624.
[16]廖瀚,甄宇红,丁超,等.槲皮素的生物活性机制及药动学特点[J].现代生物医学进展,2012,12(16):3174-3177.
[17]秦晓蓉,张铭金,高绪娜,等.槲皮素抗菌活性的研究[J].化学与生物工程,2009,26(4):55-57.
[18]徐汉虹.植物化学保护学[M].北京:中国农业出版社,2008:117-130.