丙型肝炎病毒相关混合型冷球蛋白血症的炎症因子浓度分析
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摘要
目的探讨丙型肝炎病毒(hepatitis C virus,HCV)相关混合型冷球蛋白血症(mixed cryoglobulinemia,MC)患者的外周血炎症相关因子的表达情况。方法 30例患者经过年龄、性别、HCV抗体检测结果、冷球蛋白定性结果匹配后,纳入本研究。分别归入MC和HCV双阳性组(MC+HCV+)、MC和HCV双阴性组(MC-HCV-)、MC阳性HCV阴性组(MC-HCV+)、MC阴性HCV阳性组(MC-HCV+)。采用魏氏沉降法、毛细管电泳分型进行冷球蛋白定性、定量、分型分析,Meso Scale Discovery(MSD)超敏电化学发光多细胞因子检测试剂盒检测人白细胞介素-2(interleukin-2,IL-2),CXC趋化因子10(C-X-C motif chemokine 10,CXCL10),CXC趋化因子12(C-X-C motif chemokine 12,CXCL12),胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)。IBM SPSS 22. 0软件进行数据分析。结果 MC+组IL-2,CXCL12,TSLP浓度高于MC-组(P<0.05),HCV-MC+亚组CXCL12,TSLP浓度高于HCV-MC-亚组(P<0.05)。其他亚组内细胞因子浓度差异不具有统计学意义。结论本研究发现CXCL12,TSLP与MC有一定的关联,而与HCV感染没有相关性。这些发现为进一步深入研究HCV相关MC的发生机制提供了初步的研究基础。
Objective To investigate the serum cytokines levels in patients with Hepatitis C virus(HCV)related Mixed cryoglobulinemia(MC). Methods Matched withage, gender, HCV antibody test and the qualitative results of cryoglobulin,30 patients were enrolled in this study. They were classified as MC and HCV double positive group( MC +HCV +), MC and HCV double negative group( MC-HCV-), MC positive HCV negative group(MC-HCV+), and MC negative HCV positive group(MC-HCV+). Westergren sedimentation method and capillary electrophoresistyping techniques were adopted for the qualitative,quantitative and classificationanalysisof cryoglobulin. Meso Scale Discovery( MSD) highsensitivity electrochemical luminescence was used to mearsure the serum levels of IL-2,CXCL10,CXCL12,and TSLP.We analized the data usingIBM SPSS 22.0 software. Results The IL-2,CXCL12 and TSLP concentrations in MC + group were higher than that of MC-group(P < 0. 05), and the CXCL12 and TSLP concentrationsin HCV-MC + subgroupwere higher than that of HCV-MC-subgroup(P < 0. 05). There wereno statistical differences in the serum levels of cytokines between other subgroups. Conclusion CXCL12,TSLP concentrations are associated with MC,butare not related to HCV infection. These findings provide a preliminary basis for further research on the pathogensis of HCV related MC.
Objective To investigate the serum cytokines levels in patients with Hepatitis C virus(HCV)related Mixed cryoglobulinemia(MC). Methods Matched withage, gender, HCV antibody test and the qualitative results of cryoglobulin,30 patients were enrolled in this study. They were classified as MC and HCV double positive group( MC +HCV +), MC and HCV double negative group( MC-HCV-), MC positive HCV negative group(MC-HCV+), and MC negative HCV positive group(MC-HCV+). Westergren sedimentation method and capillary electrophoresistyping techniques were adopted for the qualitative,quantitative and classificationanalysisof cryoglobulin. Meso Scale Discovery( MSD) highsensitivity electrochemical luminescence was used to mearsure the serum levels of IL-2,CXCL10,CXCL12,and TSLP.We analized the data usingIBM SPSS 22.0 software. Results The IL-2,CXCL12 and TSLP concentrations in MC + group were higher than that of MC-group(P < 0. 05), and the CXCL12 and TSLP concentrationsin HCV-MC + subgroupwere higher than that of HCV-MC-subgroup(P < 0. 05). There wereno statistical differences in the serum levels of cytokines between other subgroups. Conclusion CXCL12,TSLP concentrations are associated with MC,butare not related to HCV infection. These findings provide a preliminary basis for further research on the pathogensis of HCV related MC.
引文
[1] BROUET J C, CLAUVEL J P, DANON F, et al. Biologic and clinical significance of cryoglobulins:a report of 86 cases[J]. Am J Med,1974,57(5):775-788.
[2] SANSONNO D,DAMMACCO F. Hepatitis C virus,cryoglobulinaemia,and vasculitis:immune complex relations[J]. Lancet Infect Dis,2005,5(4):227-236.
[3] 2016年全国法定传染病疫情概况[EB/OL].http://www.nhfpc.gov. cn.
[4] PILERI P, UEMATSU Y, CAMPAGNOLI S, et al. Binding of hepatitis C virus to CD81[J]. Science, 1998, 282(5390):938-941.
[5]王宇,张颖,贾战生,等.丙型肝炎病毒相关性混合型冷球蛋白血症的治疗进展[J].临床肝胆病杂志,2012,28(8):628-632.
[6] NATTERMANN J, NISCHALKE H D, FELDMANN G, et al.Binding of HCV E2 to CD81 induces RANTES secretion and internalization of CC chemokine receptor 5[J]. J Viral Hepat,2004,11(6):519-526.
[7] D'AGOSTO G,TRENTO E,NOSOTTIL,et al. CD81 expression on CD19+peripheral blood lymphocytes is associated with chronic HCV disease and increased risk for HCV infection:a putative role for inflammatory cytokines[J]. J Biol Regul Homeost Agents,2009,23(3):155-164.
[8] LOFFREDA S, MURATORI P, MURATORI L, et al. Enhanced monocyte Thl cytokine production in HCV-infected cryoglobulinemic patients[J]. J Hepatol,2003,38(2):230-236.
[9] APOLINARIO A, MAJANO P L, LORENTE R, et al. Gene expression profile of T-cell-specific chemokines in human hepatocyte-derived cells:evidence for a synergistic inducer effect of cytokines and hepatitis C virus proteins[J]. J Viral Hepat, 2005,12(1):27-37.
[10] SAADOUN D, BOYER O, TREBEDEN-NEGRE H, et al.Predominance of type 1(Th1)cytokine production in the liver of patients with HCV-associated mixed cryoglobulinemia vasculitis[J].J Hepatol, 2004,41(6):1031-1037.
[11]范晓红,邓志华,徐小元.慢性丙型病毒性肝炎冷球蛋白血症患者血清CXC趋化因子10水平及其对抗病毒治疗的影响[J].中华肝脏病杂志,2016,24(10):744-748.
[12] MOSER B, LOETSCHER P. Lymphocyte traffic control by chemokines[J]. Nat Immunol,2001,2(2):123-128.
[13] PAVLASOVA G,BORSKY M, SEDA V, et al. Ibrutinib inhibits CD20 upregulation on CLL B cells mediated by the CXCR4/SDF-1axis[J]. Blood,2016,128(12),1609-1613.
[14] WALD 0,PAPPO 0, SAFADI R, et al. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus[J]. Eur J Immunol,2004,34(4):1164-1174.
[15] SANSONNO D,RUSSI S, SANSONNO S, et al. Thymic stromal lymphopoietin in hepatitis C virus-related cryoglobulinemic vasculitis:gene expression level and protein distribution[J].Arthritis Res Ther,2015,17(1):62.
[2] SANSONNO D,DAMMACCO F. Hepatitis C virus,cryoglobulinaemia,and vasculitis:immune complex relations[J]. Lancet Infect Dis,2005,5(4):227-236.
[3] 2016年全国法定传染病疫情概况[EB/OL].http://www.nhfpc.gov. cn.
[4] PILERI P, UEMATSU Y, CAMPAGNOLI S, et al. Binding of hepatitis C virus to CD81[J]. Science, 1998, 282(5390):938-941.
[5]王宇,张颖,贾战生,等.丙型肝炎病毒相关性混合型冷球蛋白血症的治疗进展[J].临床肝胆病杂志,2012,28(8):628-632.
[6] NATTERMANN J, NISCHALKE H D, FELDMANN G, et al.Binding of HCV E2 to CD81 induces RANTES secretion and internalization of CC chemokine receptor 5[J]. J Viral Hepat,2004,11(6):519-526.
[7] D'AGOSTO G,TRENTO E,NOSOTTIL,et al. CD81 expression on CD19+peripheral blood lymphocytes is associated with chronic HCV disease and increased risk for HCV infection:a putative role for inflammatory cytokines[J]. J Biol Regul Homeost Agents,2009,23(3):155-164.
[8] LOFFREDA S, MURATORI P, MURATORI L, et al. Enhanced monocyte Thl cytokine production in HCV-infected cryoglobulinemic patients[J]. J Hepatol,2003,38(2):230-236.
[9] APOLINARIO A, MAJANO P L, LORENTE R, et al. Gene expression profile of T-cell-specific chemokines in human hepatocyte-derived cells:evidence for a synergistic inducer effect of cytokines and hepatitis C virus proteins[J]. J Viral Hepat, 2005,12(1):27-37.
[10] SAADOUN D, BOYER O, TREBEDEN-NEGRE H, et al.Predominance of type 1(Th1)cytokine production in the liver of patients with HCV-associated mixed cryoglobulinemia vasculitis[J].J Hepatol, 2004,41(6):1031-1037.
[11]范晓红,邓志华,徐小元.慢性丙型病毒性肝炎冷球蛋白血症患者血清CXC趋化因子10水平及其对抗病毒治疗的影响[J].中华肝脏病杂志,2016,24(10):744-748.
[12] MOSER B, LOETSCHER P. Lymphocyte traffic control by chemokines[J]. Nat Immunol,2001,2(2):123-128.
[13] PAVLASOVA G,BORSKY M, SEDA V, et al. Ibrutinib inhibits CD20 upregulation on CLL B cells mediated by the CXCR4/SDF-1axis[J]. Blood,2016,128(12),1609-1613.
[14] WALD 0,PAPPO 0, SAFADI R, et al. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus[J]. Eur J Immunol,2004,34(4):1164-1174.
[15] SANSONNO D,RUSSI S, SANSONNO S, et al. Thymic stromal lymphopoietin in hepatitis C virus-related cryoglobulinemic vasculitis:gene expression level and protein distribution[J].Arthritis Res Ther,2015,17(1):62.