2017年北京地区水痘病原学监测结果分析
|
摘要
目的了解北京地区水痘–带状疱疹病毒(VZV)毒株类型及优势流行亚型,为水痘暴发疫情的防治提供依据。方法收集2017年北京地区水痘暴发疫情病例的疱疹液标本。采用实时荧光PCR方法,进行VZV及野毒株和疫苗株鉴定,根据VZV第22个开放阅读框基因片段序列,确定北京地区VZV进化关系。结果 2017年共收集到水痘病例标本316份,VZV病毒核酸阳性271份,阳性率为85.76%,均为野毒株。共获得240份VZV基因片段,其中234株毒株是clade2型,占97.50%,3株clade1型和3株clade4型,分别占1.25%。其中clade2型分布在北京地区16个区(县),clade1型和clade4型分别分布在西城区和大兴区。结论 2017年北京地区水痘病例主要由clade2型VZV引起,同时对新出现的clade1型和clade4型要加强监测。
Objective To understand the types and predominant types of varicella-zoster virus(VZV) in Beijing, 2017, and provide evidence for the prevention and control of varicella. Methods The clinical vesicular fluid was collected from the varicella cases detected in outbreak of Beijing in 2017 for the identification of wild strains and vaccine strains of VZV with real-time PCR. The phylogenetic analysis on wild VZV in Beijing was performed based on the sequences of twenty-second open reading frame gene fragment(ORF). Results Among the 316 vesicular fluid specimens from varicella cases, 271 were positive for VZV. All of these strains belonged to wild VZV strains. The gene fragments were obtained for 240 of 271 wild strains. They belonged to 3 serotypes, including 234 clade 2 strains(97.50%), 3 clade 1 strains(1.25%) and 3 clade 4 strains(1.25%). Clade 2 strains was distributed in all of 16 districts of Beijing, and clade 1 and clade 4 strains were distributed in Xicheng and Daxing districts, respectively. Conclusion The varicella cases were mainly caused by clade 2 VZV in Beijing in2017. It is necessary to strengthen the surveillance for new clade 1 and clade 4 VZV.
Objective To understand the types and predominant types of varicella-zoster virus(VZV) in Beijing, 2017, and provide evidence for the prevention and control of varicella. Methods The clinical vesicular fluid was collected from the varicella cases detected in outbreak of Beijing in 2017 for the identification of wild strains and vaccine strains of VZV with real-time PCR. The phylogenetic analysis on wild VZV in Beijing was performed based on the sequences of twenty-second open reading frame gene fragment(ORF). Results Among the 316 vesicular fluid specimens from varicella cases, 271 were positive for VZV. All of these strains belonged to wild VZV strains. The gene fragments were obtained for 240 of 271 wild strains. They belonged to 3 serotypes, including 234 clade 2 strains(97.50%), 3 clade 1 strains(1.25%) and 3 clade 4 strains(1.25%). Clade 2 strains was distributed in all of 16 districts of Beijing, and clade 1 and clade 4 strains were distributed in Xicheng and Daxing districts, respectively. Conclusion The varicella cases were mainly caused by clade 2 VZV in Beijing in2017. It is necessary to strengthen the surveillance for new clade 1 and clade 4 VZV.
引文
[1]张铁钢,陈萌,陈超,等.北京地区2008-2010年水痘病原学监测[J].中华检验医学杂志,2011,34(6):522-523.DOI:10.3760/cma.j.issn.1009-9158.2011.06.010.Zhang TG,Chen M,Chen C,et al.The pathogen surveillance for varicella in Beijing from 2008 to 2010[J].Chin J Lab Med,2011,34(6):522-523.DOI:10.3760/cma.j.issn.1009-9158.2011.06.010.
[2]陈婷婷,王官清.水痘-带状疱疹病毒基因分型研究进展[J].国际病毒学杂志,2011,18(1):23-28.DOI:10.3760/cma.j.issn.1673-4092.2011.01.005.Chen TT,Wang GQ.Progress on genotyping of varicella-zoster virus[J].Int J Virol,2011,18(1):23-28.DOI:10.3760/cma.j.issn.1673-4092.2011.01.005.
[3]Arvin AM.Varicella-zoster virus[J].Clin Microbiol Rev,1996,9(3):361-381.DOI:10.1128/CMR.9.3.361.
[4]Takahashi M,Otsuka T,Okuno Y,et al.Live vaccine used to prevent the spread of varicella in children in hospital[J].Lancet,1974,2(7892):1288-1290.DOI:10.1016/S0140-6736(74)90144-5.
[5]Loparev VN,Gonzalez A,Deleon-Carnes M,et al.Global identification of three major genotypes of varicella-zoster virus:longitudinal clustering and strategies for genotyping[J].J Virol,2004,78(15):8349-8358.DOI:10.1128/JVI.78.15.8349-8358.2004.
[6]Muir WB,Nichols R,Breuer J.Phylogenetic analysis of varicellazoster virus:evidence of intercontinental spread of genotypes and recombination[J].J Virol,2002,76(4):1971-1979.DOI:10.1128/JVI.76.4.1971-1979.2002.
[7]胡逢蛟,焦素黎,倪红霞,等.浙江省宁波市水痘病例监测及流行株基因多态性分析[J].国际流行病学传染病学杂志,2013,40(4):254-257.DOI:10.3760/cma.j.issn.1673-4149.2013.04.010.Hu FJ,Jiao SL,Ni HX,et al.Chickenpox cases monitoring and epidemic strains gene polymorphism analysis in Ningbo,Zhejiang[J].Int J Epidemiol Infect Dis,2013,40(4):254-257.DOI:10.3760/cma.j.issn.1673-4149.2013.04.010.
[8]Xu ST,Chen MK,Zheng HY,et al.Nationwide distribution of varicella-zoster virus clades in China[J].BMC Infect Dis,2016,16(1):542.DOI:10.1186/s12879-016-1863-x.
[9]曹一鸥,向妮娟.2007年全国水痘流行病学分析[J].疾病监测,2009,24(3):172-174.DOI:10.3784/j.issn.1003-9961.2009.03.06.Cao YO,Xiang NJ.Varicella epidemiology in China,2007[J].Dis Surveill,2009,24(3):172-174.DOI:10.3784/j.issn.1003-9961.2009.03.06.
[10]王秀云,朱宗龙,王涛,等.2012-2016年北京市昌平区水痘病例流行病学特征分析[J].国际病毒学杂志,2018,25(2):113-117.DOI:10.3760/cma.j.issn.1673-4092.2018.02.010.Wang XY,Zhu ZL,Wang T,et al.Epidemiological characteristics of varicella in Changping district of Beijing,2012-2016[J].Int JVirol,2018,25(2):113-117.DOI:10.3760/cma.j.issn.1673-4092.2018.02.010.
[11]董彦宏,时念民,李丽,等.北京市朝阳区健康人群水痘-带状疱疹病毒抗体水平调查[J].中国生物制品学杂志,2017,30(5):509-513.DOI:10.13200/j.cnki.cjb.001736.Dong YH,Shi NM,Li L,et al.Investigation on varicella-zoster virus antibody level in healthy population in Chaoyang district,Beijing city,China[J].Chin J Biol,2017,30(5):509-513.DOI:10.13200/j.cnki.cjb.001736.
[12]彭彦茜,赵文健,陈福春,等.水痘带状疱疹病毒流行的主要基因型[J].中外医学研究,2015,13(25):143-144.DOI:10.14033/j.cnki.cfmr.2015.25.069.Peng YX,Zhao WJ,Chen FC,et al.The major genotype of varicella zoster virus[J].Chin Foreign Med Res,2015,13(25):143-144.DOI:10.14033/j.cnki.cfmr.2015.25.069.
[13]杨吉星,居丽雯,施强,等.上海地区水痘带状疱疹病毒基因型研究[J].中国卫生检验杂志,2008,18(2):223-224.DOI:10.3969/j.issn.1004-8685.2008.02.011.Yang JX,Ju LW,Shi Q,et al.Research of genotype of varicellazoster virus in Shanghai[J].Chin J Health Lab Technol,2008,18(2):223-224.DOI:10.3969/j.issn.1004-8685.2008.02.011.
[14]刘静静,王明丽,甘霖,等.水痘-带状疱疹病毒临床分离株基因型别分析以及与Oka疫苗株的区别[J].中华微生物学和免疫学杂志,2009,29(3):224-228.DOI:10.3760/cma.j.issn.0254-5101.2009.03.010.Liu JJ,Wang ML,Gan L,et al.Genotyping of wild-type strains of varicella-zoster virus and their differentiation from Oka vaccine strain[J].Chin J Microbiol Immunol,2009,29(3):224-228.DOI:10.3760/cma.j.issn.0254-5101.2009.03.010.
[2]陈婷婷,王官清.水痘-带状疱疹病毒基因分型研究进展[J].国际病毒学杂志,2011,18(1):23-28.DOI:10.3760/cma.j.issn.1673-4092.2011.01.005.Chen TT,Wang GQ.Progress on genotyping of varicella-zoster virus[J].Int J Virol,2011,18(1):23-28.DOI:10.3760/cma.j.issn.1673-4092.2011.01.005.
[3]Arvin AM.Varicella-zoster virus[J].Clin Microbiol Rev,1996,9(3):361-381.DOI:10.1128/CMR.9.3.361.
[4]Takahashi M,Otsuka T,Okuno Y,et al.Live vaccine used to prevent the spread of varicella in children in hospital[J].Lancet,1974,2(7892):1288-1290.DOI:10.1016/S0140-6736(74)90144-5.
[5]Loparev VN,Gonzalez A,Deleon-Carnes M,et al.Global identification of three major genotypes of varicella-zoster virus:longitudinal clustering and strategies for genotyping[J].J Virol,2004,78(15):8349-8358.DOI:10.1128/JVI.78.15.8349-8358.2004.
[6]Muir WB,Nichols R,Breuer J.Phylogenetic analysis of varicellazoster virus:evidence of intercontinental spread of genotypes and recombination[J].J Virol,2002,76(4):1971-1979.DOI:10.1128/JVI.76.4.1971-1979.2002.
[7]胡逢蛟,焦素黎,倪红霞,等.浙江省宁波市水痘病例监测及流行株基因多态性分析[J].国际流行病学传染病学杂志,2013,40(4):254-257.DOI:10.3760/cma.j.issn.1673-4149.2013.04.010.Hu FJ,Jiao SL,Ni HX,et al.Chickenpox cases monitoring and epidemic strains gene polymorphism analysis in Ningbo,Zhejiang[J].Int J Epidemiol Infect Dis,2013,40(4):254-257.DOI:10.3760/cma.j.issn.1673-4149.2013.04.010.
[8]Xu ST,Chen MK,Zheng HY,et al.Nationwide distribution of varicella-zoster virus clades in China[J].BMC Infect Dis,2016,16(1):542.DOI:10.1186/s12879-016-1863-x.
[9]曹一鸥,向妮娟.2007年全国水痘流行病学分析[J].疾病监测,2009,24(3):172-174.DOI:10.3784/j.issn.1003-9961.2009.03.06.Cao YO,Xiang NJ.Varicella epidemiology in China,2007[J].Dis Surveill,2009,24(3):172-174.DOI:10.3784/j.issn.1003-9961.2009.03.06.
[10]王秀云,朱宗龙,王涛,等.2012-2016年北京市昌平区水痘病例流行病学特征分析[J].国际病毒学杂志,2018,25(2):113-117.DOI:10.3760/cma.j.issn.1673-4092.2018.02.010.Wang XY,Zhu ZL,Wang T,et al.Epidemiological characteristics of varicella in Changping district of Beijing,2012-2016[J].Int JVirol,2018,25(2):113-117.DOI:10.3760/cma.j.issn.1673-4092.2018.02.010.
[11]董彦宏,时念民,李丽,等.北京市朝阳区健康人群水痘-带状疱疹病毒抗体水平调查[J].中国生物制品学杂志,2017,30(5):509-513.DOI:10.13200/j.cnki.cjb.001736.Dong YH,Shi NM,Li L,et al.Investigation on varicella-zoster virus antibody level in healthy population in Chaoyang district,Beijing city,China[J].Chin J Biol,2017,30(5):509-513.DOI:10.13200/j.cnki.cjb.001736.
[12]彭彦茜,赵文健,陈福春,等.水痘带状疱疹病毒流行的主要基因型[J].中外医学研究,2015,13(25):143-144.DOI:10.14033/j.cnki.cfmr.2015.25.069.Peng YX,Zhao WJ,Chen FC,et al.The major genotype of varicella zoster virus[J].Chin Foreign Med Res,2015,13(25):143-144.DOI:10.14033/j.cnki.cfmr.2015.25.069.
[13]杨吉星,居丽雯,施强,等.上海地区水痘带状疱疹病毒基因型研究[J].中国卫生检验杂志,2008,18(2):223-224.DOI:10.3969/j.issn.1004-8685.2008.02.011.Yang JX,Ju LW,Shi Q,et al.Research of genotype of varicellazoster virus in Shanghai[J].Chin J Health Lab Technol,2008,18(2):223-224.DOI:10.3969/j.issn.1004-8685.2008.02.011.
[14]刘静静,王明丽,甘霖,等.水痘-带状疱疹病毒临床分离株基因型别分析以及与Oka疫苗株的区别[J].中华微生物学和免疫学杂志,2009,29(3):224-228.DOI:10.3760/cma.j.issn.0254-5101.2009.03.010.Liu JJ,Wang ML,Gan L,et al.Genotyping of wild-type strains of varicella-zoster virus and their differentiation from Oka vaccine strain[J].Chin J Microbiol Immunol,2009,29(3):224-228.DOI:10.3760/cma.j.issn.0254-5101.2009.03.010.