荷叶生物碱成分及其调脂机制研究进展
|
摘要
荷叶Nelumbo nucifera是《本草纲目》记载具有调脂减肥功效的药材。荷叶生物碱是从荷叶中提取的生物碱类活性物质,具有抑菌、调脂、止痉等多种功效。近年来因肥胖引发的健康问题受到高度重视,因此荷叶生物碱的调脂减肥功效成为研究热点。对荷叶生物碱的成分、调脂机制及其在药品开发中的应用进行综述,为荷叶生物碱调脂产品的开发及调脂机制的深入研究提供参考。
Nelumbinis Folium is a Chinese herbal medicine with the effect of regulating lipid and losing weight recorded in "Compendium of Materia Medica". The alkaloids extracted from lotus leaves have bacteriostatic, lipid-lowering, antispasmodic and other biological activities. In recent years, much attention has been paid to the health problems caused by obesity, so the effect of alkaloids in Nelumbinis Folium on lowering fat and losing weight has become a research hotspot. The chemical constituents, lipid-lowering mechanisms and application in medicine of alkaloids in Nelumbinis Folium were reviewed, which provides a theoretical reference for the development of slimming products and lipid-lowering mechanism of alkaloids in Nelumbinis Folium.
Nelumbinis Folium is a Chinese herbal medicine with the effect of regulating lipid and losing weight recorded in "Compendium of Materia Medica". The alkaloids extracted from lotus leaves have bacteriostatic, lipid-lowering, antispasmodic and other biological activities. In recent years, much attention has been paid to the health problems caused by obesity, so the effect of alkaloids in Nelumbinis Folium on lowering fat and losing weight has become a research hotspot. The chemical constituents, lipid-lowering mechanisms and application in medicine of alkaloids in Nelumbinis Folium were reviewed, which provides a theoretical reference for the development of slimming products and lipid-lowering mechanism of alkaloids in Nelumbinis Folium.
引文
[1]杨菲,徐新,唐良秋,等.荷叶生物碱的研究进展[J].现代医院,2012,12(6):93-94.
[2]朱秀萍,徐翔.荷叶生物碱研究进展[J].中国药房,2008,19(6):459-461.
[3]Ghias U,Abdur R,Bina S S,et al.Anti-nociceptive,anti-inflammatory and sedative activities of the extracts and chemical constituents of Diospyros lotus L.[J].Phytomedicine,2014,21(7):954-959.
[4]Mahboba B N,Rabia O A,Nabil A S,et al.Evaluation of antibacterial and antioxidant activities of Artemisia campestris(Astraceae)and Ziziphus lotus(Rhamnacea)[J].Arab J Chem,2010,3(2):79-84.
[5]蒋益虹.荷叶抑菌有效成分的提取优化工艺研究[J].中国中药杂志,2005,30(13):1030-1031.
[6]Jeong S Y,Yun J L,Kyoung S K,et al.Ethanol extract of lotus(Nelumbo nucifera)root exhibits an anti-adipogenic effect in human pre-adipocytes and anti-obesity and anti-oxidant effects in rats fed a high-fat diet[J].Nutr Res,2014,34(3):258-267.
[7]Ivette M M,Peter J F.Benzylisoquinoline alkaloids biosynthesis in sacred lotus[J].Molecules,2018,doi:10.3390/molecules23112899.
[8]福田真雄,渡边真男,福田真次,等.莲的生物碱(第1报)[J].药学杂志(日),1961,81(4):469.
[9]范婷婷.荷叶生物碱类物质降脂减肥活性研究[D].杭州:浙江大学,2013.
[10]王颖滢.荷叶生物碱单体的分离鉴定及其抑菌活性研究[D].杭州:浙江大学,2012.
[11]Liu C M,Kao C L,Wu H M,et al.Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn.[J].Molecules,2014,19(11):17829-17838.
[12]Nakamura S,Nakashima S,Tanabe G,et al.Alkaloid constitutens from flower buds and leaves of sacred lotus(Nelumbo nucifera,Nymphaeaceae)with melanogenesis inhibitory activity in B16 melanoma cells[J].Med Chem,2013,doi:10.1016/j.bmc.2012.11.038.
[13]周家驹,谢桂荣,严新建.中药原植物化学成分集[M].北京:科学出版社,2009.
[14]陈曦,戚进.荷叶中黄酮和生物碱的研究进展[J].中国实验方剂学杂志,2015,21(18):211-214.
[15]吴昊,刘斌,王伟,等.荷叶中的一个新阿朴啡型生物碱[J].中草药,2010,41(4):514-516.
[16]罗金波,肖文军,刘仲华.荷叶生物碱类成分的研究进展[J].今日药学,2008,18(3):9-12.
[17]袁谱龙,陈亮,刘小宇,等.荷叶生物碱分离及相关活性研究[J].中成药,2014,36(11):2330-2333.
[18]王洪燕,周先礼,黄帅,等.凹叶厚朴中生物碱成分的研究[J].华西药学杂志,2007,22(1):30-33.
[19]Guo Y,Chen X,Qi J,et al.Simultaneous qualitative and quantitative analysis of flavonoids and alkaloids from the leaves of Nelumbo nucifera Gaertn.using highperformance liquid chromatography with quadrupole time-of-flight mass spectrometry[J].J Sep Sci,2016,39(13):2499-2507.
[20]郑振佳,王晓,王明林,等.固相萃取-快速分离液相-四级杆串联飞行时间质谱联用分析荷叶中的生物碱[J].中草药,2011,42(6):1066-1068.
[21]Deng X,Zhu L,Fang T,et al.Analysis of isoquinoline alkaloid composition and wound-induced variation in Nelumbo using HPLC-MS/MS[J].J Agric Food Chem,2016,64(5):1130-1136.
[22]黄阿根,施洪飞,韦红,等.荷叶黄酮和生物碱的提纯及调节血脂作用比较[J].扬州大学烹饪学报,2006(3):23-25.
[23]涂长春,李晓宇,杨军平,等.荷叶生物总碱对肥胖高脂血症大鼠减肥作用的实验研究[J].江西中医学院学报,2001,13(3):120-121.
[24]Sharma B R,Gautam L N S,Adhikari D,et al.Acomprehensive review on chemical profiling of Nelumbo nucifera:Potential for drug development[J].Phytother Res,doi:10.1002/ptr.5732.
[25]张丽静,艾耀伟,王政强.荷叶碱防治小鼠高脂血症作用及其机制[J].医药导报,2015,34(4):440-444.
[26]范婷婷,法鲁克,方芳,等.荷叶总生物碱降脂减肥作用的体内外试验[J].浙江大学学报:农业与生命科学版,2013,39(2):141-148.
[27]赵骏,高岚,齐召朋.荷叶总生物碱及其盐的提取和降脂作用的比较[J].天津中医药,2005,22(2):161-162.
[28]Jong H A,Eun S K,Chul L,et al.Chemical constituents from Nelumbo nucifera leaves and their anti-obesity effects[J].Bioorg Med Chem Lett,2013,23(12):3604-3608.
[29]袁洋.非酒精性脂肪性肝病的代谢组学及荷叶生物碱干预机制研究[D].扬州:扬州大学,2008.
[30]孙晨光.论荷叶的减肥降脂作用[J].中医临床研究,2014,6(3):100-102.
[31]Guo F C,Yang X,Li X X,et al.Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters[J].PLo S One,2013,8(5):e63770.
[32]韩晓,吴成爱,王伟,等.荷叶碱对bel-7402细胞胆固醇代谢的影响[J].现代生物医学进展,2008,8(9):1628-1630.
[33]刘晓琴,郭慧,晁鲁平,等.荷叶生物碱对3T3-L1前脂肪细胞增殖分化的影响[J].中国食品学报,2016,16(10):54-58.
[34]刘晓琴.荷叶生物碱单体的分离纯化及其对3T3-L1前脂肪细胞作用的构效关系研究[D].杭州:浙江大学,2015.
[35]Ma C J,Wang J J,Chu H M,et al.Purification and characterization of aporphine alkaloids from leaves of Nelumbo nucifera Gaertn.and their effects on glucose consumption in 3T3-L1 adipocytes[J].Inter J Mol Sci,2014,15(3):3481-3494.
[36]Zhang D D,Zhang J G,Wu X,et al.Nuciferine downregulates Per-Arnt-Sim kinase expression during its alleviation of lipogenesis and inflammation on oleic acid-induced hepatic steatosis in HepG(2)cells[J].Front Pharmacol,2015,doi:10.3389/fphar.2015.00238.
[37]Nguyen K H,Ta T N,Pham T H M,et al.Nuciferine stimulates insulin secretion from beta cells-An in vitro comparison with glibenclamide[J].J Ethnopharmacol,2012,142(2):488-495.
[38]栗亚云.天然植物中胰脂肪酶抑制剂的筛选[D].太原:山西大学,2015.
[39]牛梦宪,隋建新,林洪,等.3种天然提取物对胰脂肪酶卵黄抗体的增效作用[J].食品科技,2018,43(7):203-209.
[40]李刚.AMPK活化介导荷叶生物碱干预脂毒性的研究[A]//第八届泛环渤海生物化学与分子生物学会2018年学术交流会论文集[C].天津:山东生物化学与分子生物学会,2018.
[41]Ma C J,Li G,He Y F,et al.Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway[J].Life Sci,2015,doi:10.1016/j.lfs.2015.07.001.
[42]Hurt R T,Edakkanambeth V J,Ebbert J O,et al.New pharmacological treatments for the management of obesity[J].Curr Gastroenterol Rep,2014,16(6):1-8.
[43]Munusamy V,Yap B K,Buckle M J C,et al.Structure-based identification of aporphines with selective 5-HT2A receptor-binding activity[J].Chem Biol Drug Design,2013,81(2):250-256.
[44]Zhang C,Deng J J,Liu D,et al.Nuciferine ameliorates hepatic steatosis in high-fat diet/streptozocin-induced diabetic mice through a PPAR alpha/PPAR gamma coactivator-1 alpha pathway[J].British J Pharmacol,2018,175(22):4218-4228.
[45]熊登科,黄志军,邓瑜,等.反相高效液相色谱法测定通脉降脂片荷叶碱含量[J].医药导报,2012,31(8):1070-1071.
[46]范成太,闫克里,赵丽,等.高效液相色谱法测定降脂宁颗粒中药材荷叶中荷叶碱的含量[J].中国药物与临床,2007,7(12):950-951.
[47]朱良辉,汪军,尧享华.高效液相色谱法测定荷丹颗粒中荷叶碱的含量[J].江西中医学院学报,2005,17(6):38.
[48]唐外姣,周本杰,周华.护肝清脂片对非酒精性脂肪肝大鼠的药效学研究[J].中药药理与临床,2013,29(2):169-172.
[49]肖淳欣,杨妙婷,陈芝娟,等.护肝清脂颗粒的质控研究[J].中药材,2018,41(6):1408-1414.
[50]柳占彪,李玉红,张少卓,等.糖脂清对高脂血症家兔糖脂代谢及肝组织的影响[J].中国实验方剂学杂志,2011,17(15):135-138.
[51]祁晓霞.糖脂清方治疗2型糖尿病合并高脂血症的临床研究[D].南京:南京中医药大学,2006.
[52]Li Z Q,Liu J,Zhang D Q,et al.Nuciferine and paeoniflorin can be quality markers of Tangzhiqing tablet,a Chinese traditional patent medicine,based on the qualitative,quantitative and dose-exposure-response analysis[J].Phytomedicine,2018,doi:10.1016/j.phymed.2018.02.006.
[2]朱秀萍,徐翔.荷叶生物碱研究进展[J].中国药房,2008,19(6):459-461.
[3]Ghias U,Abdur R,Bina S S,et al.Anti-nociceptive,anti-inflammatory and sedative activities of the extracts and chemical constituents of Diospyros lotus L.[J].Phytomedicine,2014,21(7):954-959.
[4]Mahboba B N,Rabia O A,Nabil A S,et al.Evaluation of antibacterial and antioxidant activities of Artemisia campestris(Astraceae)and Ziziphus lotus(Rhamnacea)[J].Arab J Chem,2010,3(2):79-84.
[5]蒋益虹.荷叶抑菌有效成分的提取优化工艺研究[J].中国中药杂志,2005,30(13):1030-1031.
[6]Jeong S Y,Yun J L,Kyoung S K,et al.Ethanol extract of lotus(Nelumbo nucifera)root exhibits an anti-adipogenic effect in human pre-adipocytes and anti-obesity and anti-oxidant effects in rats fed a high-fat diet[J].Nutr Res,2014,34(3):258-267.
[7]Ivette M M,Peter J F.Benzylisoquinoline alkaloids biosynthesis in sacred lotus[J].Molecules,2018,doi:10.3390/molecules23112899.
[8]福田真雄,渡边真男,福田真次,等.莲的生物碱(第1报)[J].药学杂志(日),1961,81(4):469.
[9]范婷婷.荷叶生物碱类物质降脂减肥活性研究[D].杭州:浙江大学,2013.
[10]王颖滢.荷叶生物碱单体的分离鉴定及其抑菌活性研究[D].杭州:浙江大学,2012.
[11]Liu C M,Kao C L,Wu H M,et al.Antioxidant and anticancer aporphine alkaloids from the leaves of Nelumbo nucifera Gaertn.[J].Molecules,2014,19(11):17829-17838.
[12]Nakamura S,Nakashima S,Tanabe G,et al.Alkaloid constitutens from flower buds and leaves of sacred lotus(Nelumbo nucifera,Nymphaeaceae)with melanogenesis inhibitory activity in B16 melanoma cells[J].Med Chem,2013,doi:10.1016/j.bmc.2012.11.038.
[13]周家驹,谢桂荣,严新建.中药原植物化学成分集[M].北京:科学出版社,2009.
[14]陈曦,戚进.荷叶中黄酮和生物碱的研究进展[J].中国实验方剂学杂志,2015,21(18):211-214.
[15]吴昊,刘斌,王伟,等.荷叶中的一个新阿朴啡型生物碱[J].中草药,2010,41(4):514-516.
[16]罗金波,肖文军,刘仲华.荷叶生物碱类成分的研究进展[J].今日药学,2008,18(3):9-12.
[17]袁谱龙,陈亮,刘小宇,等.荷叶生物碱分离及相关活性研究[J].中成药,2014,36(11):2330-2333.
[18]王洪燕,周先礼,黄帅,等.凹叶厚朴中生物碱成分的研究[J].华西药学杂志,2007,22(1):30-33.
[19]Guo Y,Chen X,Qi J,et al.Simultaneous qualitative and quantitative analysis of flavonoids and alkaloids from the leaves of Nelumbo nucifera Gaertn.using highperformance liquid chromatography with quadrupole time-of-flight mass spectrometry[J].J Sep Sci,2016,39(13):2499-2507.
[20]郑振佳,王晓,王明林,等.固相萃取-快速分离液相-四级杆串联飞行时间质谱联用分析荷叶中的生物碱[J].中草药,2011,42(6):1066-1068.
[21]Deng X,Zhu L,Fang T,et al.Analysis of isoquinoline alkaloid composition and wound-induced variation in Nelumbo using HPLC-MS/MS[J].J Agric Food Chem,2016,64(5):1130-1136.
[22]黄阿根,施洪飞,韦红,等.荷叶黄酮和生物碱的提纯及调节血脂作用比较[J].扬州大学烹饪学报,2006(3):23-25.
[23]涂长春,李晓宇,杨军平,等.荷叶生物总碱对肥胖高脂血症大鼠减肥作用的实验研究[J].江西中医学院学报,2001,13(3):120-121.
[24]Sharma B R,Gautam L N S,Adhikari D,et al.Acomprehensive review on chemical profiling of Nelumbo nucifera:Potential for drug development[J].Phytother Res,doi:10.1002/ptr.5732.
[25]张丽静,艾耀伟,王政强.荷叶碱防治小鼠高脂血症作用及其机制[J].医药导报,2015,34(4):440-444.
[26]范婷婷,法鲁克,方芳,等.荷叶总生物碱降脂减肥作用的体内外试验[J].浙江大学学报:农业与生命科学版,2013,39(2):141-148.
[27]赵骏,高岚,齐召朋.荷叶总生物碱及其盐的提取和降脂作用的比较[J].天津中医药,2005,22(2):161-162.
[28]Jong H A,Eun S K,Chul L,et al.Chemical constituents from Nelumbo nucifera leaves and their anti-obesity effects[J].Bioorg Med Chem Lett,2013,23(12):3604-3608.
[29]袁洋.非酒精性脂肪性肝病的代谢组学及荷叶生物碱干预机制研究[D].扬州:扬州大学,2008.
[30]孙晨光.论荷叶的减肥降脂作用[J].中医临床研究,2014,6(3):100-102.
[31]Guo F C,Yang X,Li X X,et al.Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters[J].PLo S One,2013,8(5):e63770.
[32]韩晓,吴成爱,王伟,等.荷叶碱对bel-7402细胞胆固醇代谢的影响[J].现代生物医学进展,2008,8(9):1628-1630.
[33]刘晓琴,郭慧,晁鲁平,等.荷叶生物碱对3T3-L1前脂肪细胞增殖分化的影响[J].中国食品学报,2016,16(10):54-58.
[34]刘晓琴.荷叶生物碱单体的分离纯化及其对3T3-L1前脂肪细胞作用的构效关系研究[D].杭州:浙江大学,2015.
[35]Ma C J,Wang J J,Chu H M,et al.Purification and characterization of aporphine alkaloids from leaves of Nelumbo nucifera Gaertn.and their effects on glucose consumption in 3T3-L1 adipocytes[J].Inter J Mol Sci,2014,15(3):3481-3494.
[36]Zhang D D,Zhang J G,Wu X,et al.Nuciferine downregulates Per-Arnt-Sim kinase expression during its alleviation of lipogenesis and inflammation on oleic acid-induced hepatic steatosis in HepG(2)cells[J].Front Pharmacol,2015,doi:10.3389/fphar.2015.00238.
[37]Nguyen K H,Ta T N,Pham T H M,et al.Nuciferine stimulates insulin secretion from beta cells-An in vitro comparison with glibenclamide[J].J Ethnopharmacol,2012,142(2):488-495.
[38]栗亚云.天然植物中胰脂肪酶抑制剂的筛选[D].太原:山西大学,2015.
[39]牛梦宪,隋建新,林洪,等.3种天然提取物对胰脂肪酶卵黄抗体的增效作用[J].食品科技,2018,43(7):203-209.
[40]李刚.AMPK活化介导荷叶生物碱干预脂毒性的研究[A]//第八届泛环渤海生物化学与分子生物学会2018年学术交流会论文集[C].天津:山东生物化学与分子生物学会,2018.
[41]Ma C J,Li G,He Y F,et al.Pronuciferine and nuciferine inhibit lipogenesis in 3T3-L1 adipocytes by activating the AMPK signaling pathway[J].Life Sci,2015,doi:10.1016/j.lfs.2015.07.001.
[42]Hurt R T,Edakkanambeth V J,Ebbert J O,et al.New pharmacological treatments for the management of obesity[J].Curr Gastroenterol Rep,2014,16(6):1-8.
[43]Munusamy V,Yap B K,Buckle M J C,et al.Structure-based identification of aporphines with selective 5-HT2A receptor-binding activity[J].Chem Biol Drug Design,2013,81(2):250-256.
[44]Zhang C,Deng J J,Liu D,et al.Nuciferine ameliorates hepatic steatosis in high-fat diet/streptozocin-induced diabetic mice through a PPAR alpha/PPAR gamma coactivator-1 alpha pathway[J].British J Pharmacol,2018,175(22):4218-4228.
[45]熊登科,黄志军,邓瑜,等.反相高效液相色谱法测定通脉降脂片荷叶碱含量[J].医药导报,2012,31(8):1070-1071.
[46]范成太,闫克里,赵丽,等.高效液相色谱法测定降脂宁颗粒中药材荷叶中荷叶碱的含量[J].中国药物与临床,2007,7(12):950-951.
[47]朱良辉,汪军,尧享华.高效液相色谱法测定荷丹颗粒中荷叶碱的含量[J].江西中医学院学报,2005,17(6):38.
[48]唐外姣,周本杰,周华.护肝清脂片对非酒精性脂肪肝大鼠的药效学研究[J].中药药理与临床,2013,29(2):169-172.
[49]肖淳欣,杨妙婷,陈芝娟,等.护肝清脂颗粒的质控研究[J].中药材,2018,41(6):1408-1414.
[50]柳占彪,李玉红,张少卓,等.糖脂清对高脂血症家兔糖脂代谢及肝组织的影响[J].中国实验方剂学杂志,2011,17(15):135-138.
[51]祁晓霞.糖脂清方治疗2型糖尿病合并高脂血症的临床研究[D].南京:南京中医药大学,2006.
[52]Li Z Q,Liu J,Zhang D Q,et al.Nuciferine and paeoniflorin can be quality markers of Tangzhiqing tablet,a Chinese traditional patent medicine,based on the qualitative,quantitative and dose-exposure-response analysis[J].Phytomedicine,2018,doi:10.1016/j.phymed.2018.02.006.