丝氨酸/精氨酸富集剪接因子1和Livin在膀胱移行细胞癌中的表达及临床意义
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摘要
目的探讨膀胱移行细胞癌组织中丝氨酸/精氨酸富集剪接因子1(serine/arginine-rich splicing factor 1,SRSF1)和凋亡蛋白抑制因子Livin表达及临床意义。方法膀胱移行细胞癌患者46例,取手术切除癌组织46份为膀胱移行细胞癌组,癌旁正常组织10份为对照组,采用免疫组织化学法检测2组SRSF1和Livin阳性表达情况,分析SRSF1和Livin阳性表达与膀胱移行细胞癌临床病理特征的关系,Spearman秩相关分析膀胱移行细胞癌患者SRSF1和Livin阳性表达的相关性。结果膀胱移行细胞癌组SRSF1、Livin阳性表达率(80.4%、82.6%)均高于对照组(10.0%、30.0%)(P<0.05);膀胱移行细胞癌组肿瘤复发者SRSF1、Livin阳性表达率(92.6%、96.3%)高于未复发者(63.2%、63.2%)(P<0.05),SRSF1、Livin阳性表达率在肿瘤是否单发、分化程度、TNM分期上比较差异无统计学意义(P>0.05);Spearman秩相关分析结果显示,膀胱移行细胞癌组SRSF1和Livin阳性表达呈正相关(r=0.662,P<0.001)。结论膀胱移行细胞癌组织中Livin、SRSF1呈高表达,且与患者复发有关。
Objective To investigate the expressions of serine/arginine-rich splicing factor 1(SRSF1)and apoptotic inhibitor Livin in bladder transitional cell carcinoma(BTCC)and their clinical significances.Methods The expressions of SRSF1 and Livin were detected by immunohistochemical method in 46 samples of resected BTCC tissues(BTCC group)and 10 samples of adjacent normal tissues(control group)from 46 patients with BTCC.The relationship of SRSF1 and Livin expressions with the clinicopathological characteristics was analyzed.The Spearman method was used to analyze the correlation between SRSF1 and Livin.Results The positive rates of SRSF1 and Livin were significantly higher in BTCC group(80.4%,82.6%)than those in control group(10.0%,30.0%)(P<0.05).The positive rates of SRSF1 and Livin were significantly higher in recurrent patients(92.6%,96.3%)than those in patients without recurrence(63.2%,63.2%)in BTCC group(P<0.05).The positive rates of SRSF1 and Livin showed no significant differences in single or multiple tumor,differentiation degree and TNM stage(P>0.05).Spearman rank correlation analysis showed that the expressions of SRSF1 and Livin protein were positively correlated with BTCC(r=0.662,P<0.001).Conclusion Livin and SRSF1 are highly expressed in BTCC and are involved in its recurrence.
Objective To investigate the expressions of serine/arginine-rich splicing factor 1(SRSF1)and apoptotic inhibitor Livin in bladder transitional cell carcinoma(BTCC)and their clinical significances.Methods The expressions of SRSF1 and Livin were detected by immunohistochemical method in 46 samples of resected BTCC tissues(BTCC group)and 10 samples of adjacent normal tissues(control group)from 46 patients with BTCC.The relationship of SRSF1 and Livin expressions with the clinicopathological characteristics was analyzed.The Spearman method was used to analyze the correlation between SRSF1 and Livin.Results The positive rates of SRSF1 and Livin were significantly higher in BTCC group(80.4%,82.6%)than those in control group(10.0%,30.0%)(P<0.05).The positive rates of SRSF1 and Livin were significantly higher in recurrent patients(92.6%,96.3%)than those in patients without recurrence(63.2%,63.2%)in BTCC group(P<0.05).The positive rates of SRSF1 and Livin showed no significant differences in single or multiple tumor,differentiation degree and TNM stage(P>0.05).Spearman rank correlation analysis showed that the expressions of SRSF1 and Livin protein were positively correlated with BTCC(r=0.662,P<0.001).Conclusion Livin and SRSF1 are highly expressed in BTCC and are involved in its recurrence.
引文
[1]SIEGEL R L,MILLER K D,JEMAL A.Cancer statistics,2015[J].CA Cancer J Clin,2015,65(1):5-29.
[2]祝兴旺,张明明,宋永胜.凋亡抑制蛋白Livin在膀胱移行细胞癌中表达及临床意义[J].中华实用诊断与治疗杂志,2009,23(2):121-123.
[3]郑巍,桂西青,苟新敏,等.膀胱移行细胞癌患者尿Survivin及透明质酸含量检测意义[J].中华实用诊断与治疗杂志,2008,22(6):407-408.
[4]何广宁,凌晓辉,江福能,等.丝氨酸/精氨酸富集剪接因子1上调表达对前列腺癌发病进程及临床预后的指导作用[J].中华实验外科杂志,2015,32(5):1187-1189.
[5]LEU S,LIN Y M,WU C H,et al.Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD[J].J Cell Sci,2012,125(Pt 13):3164-3172.
[6]张远贵,段冬,李攀登,等.瘢痕疙瘩组织中Livin、Smac、Caspase-3的表达及其相关性[J].中国组织工程研究,2016,20(11):1558-1563.
[7]张寒,郑胡镛.丝氨酸/精氨酸富集剪接因子1在肿瘤发生发展过程中的作用[J].中国肿瘤生物治疗杂志,2013,20(6):631-636.
[8]肖邦明,祝兴旺,董理鸣,等.SRSF1和Survivin在前列腺癌组织中的表达及其与病理分级的相关性[J].中国医科大学学报,2016,45(5):452-455.
[9]祝兴旺,李永智,刘屹立.SRSF1和caspase-3在膀胱尿路上皮癌中的表达及其临床意义[J].现代肿瘤医学,2017,25(3):412-415.
[10]RICHTER B W,MIR S S,EIBEN L J,et al.Molecular cloning of ILP-2,a novel member of the inhibitor of apoptosis protein family[J].Mol Cell Biol,2001,21(13):4292-4301.
[11]张应选,赵永福.Livin在原发性肝癌中的表达与caspase-3的关系[J].中国实用医刊,2010,37(8):13-14.
[12]张建成,吴刚,康谊,等.Livin和P53蛋白在胃癌及癌旁组织中的表达及临床意义[J].中华实用诊断与治疗杂志,2012,26,(9):876.
[13]刘军辉,赵永灵,喻军.Livin蛋白表达与结直肠癌组织病理参数间关系研究[J].中华实用诊断与治疗杂志,2015,29(12):1160-1162.
[14]高伟兴,张新,张立国,等.膀胱尿路上皮癌组织中Livin蛋白的表达与预后的关系[J].西部医学,2011,23(2):236-238.
[15]李国伟.上皮性膀胱癌组织中Livin蛋白表达与肿瘤复发的关系[J].肿瘤基础与临床,2015,28(5):386-387.
[2]祝兴旺,张明明,宋永胜.凋亡抑制蛋白Livin在膀胱移行细胞癌中表达及临床意义[J].中华实用诊断与治疗杂志,2009,23(2):121-123.
[3]郑巍,桂西青,苟新敏,等.膀胱移行细胞癌患者尿Survivin及透明质酸含量检测意义[J].中华实用诊断与治疗杂志,2008,22(6):407-408.
[4]何广宁,凌晓辉,江福能,等.丝氨酸/精氨酸富集剪接因子1上调表达对前列腺癌发病进程及临床预后的指导作用[J].中华实验外科杂志,2015,32(5):1187-1189.
[5]LEU S,LIN Y M,WU C H,et al.Loss of Pnn expression results in mouse early embryonic lethality and cellular apoptosis through SRSF1-mediated alternative expression of Bcl-xS and ICAD[J].J Cell Sci,2012,125(Pt 13):3164-3172.
[6]张远贵,段冬,李攀登,等.瘢痕疙瘩组织中Livin、Smac、Caspase-3的表达及其相关性[J].中国组织工程研究,2016,20(11):1558-1563.
[7]张寒,郑胡镛.丝氨酸/精氨酸富集剪接因子1在肿瘤发生发展过程中的作用[J].中国肿瘤生物治疗杂志,2013,20(6):631-636.
[8]肖邦明,祝兴旺,董理鸣,等.SRSF1和Survivin在前列腺癌组织中的表达及其与病理分级的相关性[J].中国医科大学学报,2016,45(5):452-455.
[9]祝兴旺,李永智,刘屹立.SRSF1和caspase-3在膀胱尿路上皮癌中的表达及其临床意义[J].现代肿瘤医学,2017,25(3):412-415.
[10]RICHTER B W,MIR S S,EIBEN L J,et al.Molecular cloning of ILP-2,a novel member of the inhibitor of apoptosis protein family[J].Mol Cell Biol,2001,21(13):4292-4301.
[11]张应选,赵永福.Livin在原发性肝癌中的表达与caspase-3的关系[J].中国实用医刊,2010,37(8):13-14.
[12]张建成,吴刚,康谊,等.Livin和P53蛋白在胃癌及癌旁组织中的表达及临床意义[J].中华实用诊断与治疗杂志,2012,26,(9):876.
[13]刘军辉,赵永灵,喻军.Livin蛋白表达与结直肠癌组织病理参数间关系研究[J].中华实用诊断与治疗杂志,2015,29(12):1160-1162.
[14]高伟兴,张新,张立国,等.膀胱尿路上皮癌组织中Livin蛋白的表达与预后的关系[J].西部医学,2011,23(2):236-238.
[15]李国伟.上皮性膀胱癌组织中Livin蛋白表达与肿瘤复发的关系[J].肿瘤基础与临床,2015,28(5):386-387.