丙酮丁醇梭菌复合诱变及发酵废弃物原料产生物丁醇的研究
|
摘要
[目的]通过废弃资源的再利用研究可替代的新型生物燃料——丁醇的发酵培养基。[方法]对菌株Clostridium acetobutylicum CGMCC 1.0134进行紫外诱变和磁场与Fe~(2+)共同诱变,获得1株丁醇产量高和稳定性好的优良突变株Clostridium acetobutylicum UM-80,采用不同的废弃原料考察该突变菌株的发酵性能,并筛选出合适的性价比高的培养基。[结果]突变株UM-80发酵产丁醇和总溶剂(丙酮、丁醇、乙醇)分别为9.04、17.95 g/L,较原始菌株分别提高了5.12%、4.12%。单独的蕨根是较好的丁醇发酵原料,当蕨根醪质量分数为15%时发酵液中丁醇浓度最高为5.50 g/L。当高山被孢霉与蕨根共同发酵时发酵液中丁醇最高产量为6.50 g/L。[结论]蕨根与高山被孢霉的复合培养基是较好的生物丁醇发酵培养基。
[Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources.[Method]Clostridium acetobutylicum UM-80,a mutant with high butanol yield and good stability,was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe~(2+) by Clostridium acetobutylicum CGMCC 1. 0134. Then the different fermentation materials were used to study the fermentation performance of the mutant strains,and the suitable medium with high cost performance was found out. [Result]The yield of butanol and total solvent( acetone,butanol,ethanol) were 9. 04 and 17. 95 g/L,respectively,which were 5. 12% and 4. 12% higher than those of the original strain. The fern root was the best raw material for the fermentation of butanol; when the mass fraction of fern root was 15%,the concentration of butanol in fermentation broth was 5. 50 g/L. When the co-fermentation of Mortierella alpina and fern root,the highest yield was 6. 50 g/L. [Conclusion] The co-fermentation of Mortierella alpina and fern root is better fermentation medium for butanol.
[Objective] The aim was to study better fermentation medium for new type biofuel butanol through recycling waster resources.[Method]Clostridium acetobutylicum UM-80,a mutant with high butanol yield and good stability,was obtained by ultraviolet mutagenesis and co-mutagenesis with Fe~(2+) by Clostridium acetobutylicum CGMCC 1. 0134. Then the different fermentation materials were used to study the fermentation performance of the mutant strains,and the suitable medium with high cost performance was found out. [Result]The yield of butanol and total solvent( acetone,butanol,ethanol) were 9. 04 and 17. 95 g/L,respectively,which were 5. 12% and 4. 12% higher than those of the original strain. The fern root was the best raw material for the fermentation of butanol; when the mass fraction of fern root was 15%,the concentration of butanol in fermentation broth was 5. 50 g/L. When the co-fermentation of Mortierella alpina and fern root,the highest yield was 6. 50 g/L. [Conclusion] The co-fermentation of Mortierella alpina and fern root is better fermentation medium for butanol.
引文
[1]陈丽杰,辛程勋,邓攀,等.丙酮丁醇梭菌发酵菊芋汁生产丁醇[J].生物工程学报,2010,26(7):991-996.
[2]范俊辉,冯文亮,邸胜苗,等.利用甜菜糖蜜补料发酵生产丁醇[J].生物加工过程,2010,8(6):6-9.
[3]王风芹,原欢,楚乐然,等.玉米秸秆水解液燃料丁醇发酵条件优化研究[J].食品与发酵工业,2010,36(10):79-83.
[4]马晓建,张霞,常春.丙酮丁醇梭菌发酵玉米秸秆生产丁醇[J].华南理工大学学报(自然科学版),2014,42(2):27-32.
[5]李汉广.高产丁醇菌株选育及其耐受丁醇机制研究[D].无锡:江南大学,2014.
[6]田毅红,朱志豪,高媛,等.红薯发酵产丁醇的工艺优化[J].化学与生物工程,2016,33(2):64-66.
[7]李鑫,李志刚,史仲平.原料碳氮比对丁醇发酵两阶段发酵性能的影响[J].食品与生物技术学报,2014,33(11):1168-1175.
[2]范俊辉,冯文亮,邸胜苗,等.利用甜菜糖蜜补料发酵生产丁醇[J].生物加工过程,2010,8(6):6-9.
[3]王风芹,原欢,楚乐然,等.玉米秸秆水解液燃料丁醇发酵条件优化研究[J].食品与发酵工业,2010,36(10):79-83.
[4]马晓建,张霞,常春.丙酮丁醇梭菌发酵玉米秸秆生产丁醇[J].华南理工大学学报(自然科学版),2014,42(2):27-32.
[5]李汉广.高产丁醇菌株选育及其耐受丁醇机制研究[D].无锡:江南大学,2014.
[6]田毅红,朱志豪,高媛,等.红薯发酵产丁醇的工艺优化[J].化学与生物工程,2016,33(2):64-66.
[7]李鑫,李志刚,史仲平.原料碳氮比对丁醇发酵两阶段发酵性能的影响[J].食品与生物技术学报,2014,33(11):1168-1175.