以甘薯为原料发酵生产丙酮-丁醇-乙醇的研究
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摘要
【目的】研究以甘薯为原料发酵生产丙酮-丁醇-乙醇(ABE)的可行性。【方法】以本研究分离鉴定的丙酮丁醇梭菌(Clostridium acetobutylicum)J-3-3为生产菌株,通过发酵培养基和发酵条件的优化,确定最佳的发酵工艺条件。发酵产物采用气相色谱的内标法测定。【结果】最佳发酵培养基组成:甘薯液化液初糖浓度为100g/L,豆粕8g/L,KH_2PO_4 0.8g/L,FeSO_4·7H_2O 0.06g/L。最佳发酵条件:温度37℃,初始pH自然(pH值为5.8),种龄22h,接种量10%。在此发酵工艺条件下发酵60h,溶剂产量达到最大,丙酮、乙醇和丁醇的产量分别为6.98g/L、1.16g/L和15.34g/L,总溶剂23.48g/L。【结论】研究结果表明,以甘薯为原料发酵生产丙酮-丁醇-乙醇是可行的,这为拓宽发酵法生产丙酮-丁醇-乙醇的原料来源以及甘薯的深加工提供了新的思路。
【Objective】The feasibility of producing acetone-butanol-ethanol(ABE)from sweet potato as raw material was studied.【Methods】ABE fermentation was carried out with Clostridium acetobutylicumJ-3-3 which was isolated and identified in this study.The optimum fermentation process was determined by optimizing the fermentation medium and the fermentation conditions.Fermentation products were determined by gas chromatography internal standard method.【Results】The optimum medium composition:The initial concentration of sugar in sweet potato liquor was 100 g/L,soybean meal was 8 g/L,KH_2PO_4 was 0.8 g/L,FeSO_4·7 H_2O was 0.06 g/L,and the initial pH was 7.5.The optimum fermentation conditions were as follows:Temperature 37℃,initial pH(pH 5.8),seedling age 22 h,and inoculation amount 10%.Under this fermentation condition,the solvent yield reached the maximum after 60 h of fermentation.The yields of acetone,ethanol and butanol were 6.98 g/L,1.16 g/L and 15.34 g/L,respectively.The total solvent was 23.48 g/L.【Conclusion】The research result demonstrated that it was feasible to perform the ABE fermentation with sweet potato as raw material,which provides a new idea of broadening the source of raw material for producing acetone-butanol-ethanol from fermentation and deep processing of sweet potato.
【Objective】The feasibility of producing acetone-butanol-ethanol(ABE)from sweet potato as raw material was studied.【Methods】ABE fermentation was carried out with Clostridium acetobutylicumJ-3-3 which was isolated and identified in this study.The optimum fermentation process was determined by optimizing the fermentation medium and the fermentation conditions.Fermentation products were determined by gas chromatography internal standard method.【Results】The optimum medium composition:The initial concentration of sugar in sweet potato liquor was 100 g/L,soybean meal was 8 g/L,KH_2PO_4 was 0.8 g/L,FeSO_4·7 H_2O was 0.06 g/L,and the initial pH was 7.5.The optimum fermentation conditions were as follows:Temperature 37℃,initial pH(pH 5.8),seedling age 22 h,and inoculation amount 10%.Under this fermentation condition,the solvent yield reached the maximum after 60 h of fermentation.The yields of acetone,ethanol and butanol were 6.98 g/L,1.16 g/L and 15.34 g/L,respectively.The total solvent was 23.48 g/L.【Conclusion】The research result demonstrated that it was feasible to perform the ABE fermentation with sweet potato as raw material,which provides a new idea of broadening the source of raw material for producing acetone-butanol-ethanol from fermentation and deep processing of sweet potato.
引文
[1]CHIAO J S,SUN Z H.History of the acetone-butanolethanol fermentation industry in China:development of continuous production technology[J].Journal of Molecular Microbiology&Biotechnology,2007,13(1-3):12-14.
[2]EZEJI T C,QURESHI N,BLASCHEK H P.Butanol production from agricultural residues:Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation[J].Biotechnology and Bioengineering,2007,97(6):1460-1469.
[3]黄格省,李振宇,张兰波,等.生物丁醇的性能优势及技术进展[J].石化技术与应用,2012,30(3):254-259.HUANG G S,LI Z Y,ZHANG L B,et al.Performance advantage and technology progress in biobutanol[J].Petrochemical Technology&Application,2012,30(3):254-258.
[4]苏会波,李凡,彭超,等.新型生物能源丁醇的研究进展和市场现状[J].生物质化学工程,2014,48(1):37-43.SU H B,LI F,PENG C,et al.Research progress and market status of novel bioenergy-butanol[J].Biomass Chemical Engineering,2014,48(1):37-43.
[5]LI S B,GUO Y,LU F Z,et al.High-level butanol production from cassava starch by a newly isolated Clostridium acetobutylicum[J].Applied Biochemistry&Biotechnology,2015,177(4):831-841.
[6]LI S B,QIAN Y,LIANG Z W,et al.Enhanced butanol production from cassava with Clostridium acetobutylicumby genome shuffling[J].World J Microbiol Biotechnol,2016,32(4):53.
[7]PANG Z W,LU W,ZHANG H,et al.Butanol production employing fed-batch fermentation by Clostridium acetobutylicum GX01using alkali-pretreated sugarcane bagasse hydrolysed by enzymes from Thermoascus aurantiacus QS 7-2-4[J].Bioresour Technol,2016,212:82-91.
[8]周英梅.木薯与甘蔗糖蜜混合发酵产丙酮丁醇的研究[D].南宁:广西大学,2014.ZHOU Y M.Study of ABE production from cassava and molasses[D].Nanning:Guangxi University,2014.
[9]庞宗文,俸雪,吴三星,等.以新鲜马铃薯为原料发酵丙酮丁醇的研究[J].可再生能源,2012,30(11):83-86.PANG Z W,FENG X,WU S X,et al.Study of acetone and butanol production from fresh potato[J].Renewable Energy Resources,2012,30(11):83-86.
[10]陈丽杰,辛程勋,邓攀,等.丙酮丁醇梭菌发酵菊芋汁生产丁醇[J].生物工程学报,2010,26(7):991-996.CHEN L J,XIN C X,DENG P,et al.Butanol production from hydrolysate of Jerusalem artichokejuice by Clostridium acetobutylicum L7[J].Chinese Journal of Biotechnology,2010,26(7):991-996.
[11]马晓建,张霞,常春.丙酮丁醇梭菌发酵玉米秸秆生产丁醇[J].华南理工大学学报:自然科学版,2014,24(2):27-32.MA X J,ZHANG X,CHANG C.Butanol production from corn stover by fermentation with Clostridium acetobutylicum[J].Journal of South China University of Technology:Natural Science Edition,2014,24(2):27-32.
[12]王风芹,仝银杏,李传斌,等.蒸汽爆破玉米芯水解液脱毒及其发酵生产燃料丁醇[J].农业工程学报,2016,32(5):257-262.WANG F Q,TONG Y X,LI C B,et al.Hydrolysate detoxified from steam exploded corn cob and its fermentationproducing butanol fuels[J].Transactions of the Chinese Society of Agricultural Engineering,2016,32(5):257-262.
[13]龙飞,靳艳玲,赵云,等.丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012发酵鲜芭蕉芋生产丁醇[J].应用与环境生物学报,2013,19(1):54-60.LONG F,JIN Y L,ZHAO Y,et al.Optimization of butanol production from Canna edulis Ker by Clostridium acetobutylicum using response surface methodology[J].Chinese Journal of Applied and Environmental Biology,2013,19(1):54-60.
[14]田毅红,朱志豪,高媛,等.红薯发酵产丁醇的工艺优化[J].化学与生物工程,2016,33(2):64-66.TIAN Y H,ZHU Z H,GAO Y,et al.Optimization on fermentation conditions of butanol from sweet potato[J].Chemistry&Bioengineering,2016,33(2):64-66.
[15]蒋玉峰,马代夫.国家甘薯产业技术体系建设推动甘薯产业和学科发展[J].江苏师范大学学报:自然科学版,2016,34(3):23-27.JIANG Y F,MA D F.The sweet potato of China agricultural research system promotes the industrilization and disciplinary development of sweet potato[J].Journal of Jiangsu Normal University:Natural Science Edition,2016,34(3):23-27.
[16]国家发展和改革委员会.可再生能源中长期发展规划[J].可再生能源,2007,25(6):1-5.National Development and Reform Commission.Longterm development plan for renewable energy[J].Renewable Energy Resources,2007,25(6):1-5.
[2]EZEJI T C,QURESHI N,BLASCHEK H P.Butanol production from agricultural residues:Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation[J].Biotechnology and Bioengineering,2007,97(6):1460-1469.
[3]黄格省,李振宇,张兰波,等.生物丁醇的性能优势及技术进展[J].石化技术与应用,2012,30(3):254-259.HUANG G S,LI Z Y,ZHANG L B,et al.Performance advantage and technology progress in biobutanol[J].Petrochemical Technology&Application,2012,30(3):254-258.
[4]苏会波,李凡,彭超,等.新型生物能源丁醇的研究进展和市场现状[J].生物质化学工程,2014,48(1):37-43.SU H B,LI F,PENG C,et al.Research progress and market status of novel bioenergy-butanol[J].Biomass Chemical Engineering,2014,48(1):37-43.
[5]LI S B,GUO Y,LU F Z,et al.High-level butanol production from cassava starch by a newly isolated Clostridium acetobutylicum[J].Applied Biochemistry&Biotechnology,2015,177(4):831-841.
[6]LI S B,QIAN Y,LIANG Z W,et al.Enhanced butanol production from cassava with Clostridium acetobutylicumby genome shuffling[J].World J Microbiol Biotechnol,2016,32(4):53.
[7]PANG Z W,LU W,ZHANG H,et al.Butanol production employing fed-batch fermentation by Clostridium acetobutylicum GX01using alkali-pretreated sugarcane bagasse hydrolysed by enzymes from Thermoascus aurantiacus QS 7-2-4[J].Bioresour Technol,2016,212:82-91.
[8]周英梅.木薯与甘蔗糖蜜混合发酵产丙酮丁醇的研究[D].南宁:广西大学,2014.ZHOU Y M.Study of ABE production from cassava and molasses[D].Nanning:Guangxi University,2014.
[9]庞宗文,俸雪,吴三星,等.以新鲜马铃薯为原料发酵丙酮丁醇的研究[J].可再生能源,2012,30(11):83-86.PANG Z W,FENG X,WU S X,et al.Study of acetone and butanol production from fresh potato[J].Renewable Energy Resources,2012,30(11):83-86.
[10]陈丽杰,辛程勋,邓攀,等.丙酮丁醇梭菌发酵菊芋汁生产丁醇[J].生物工程学报,2010,26(7):991-996.CHEN L J,XIN C X,DENG P,et al.Butanol production from hydrolysate of Jerusalem artichokejuice by Clostridium acetobutylicum L7[J].Chinese Journal of Biotechnology,2010,26(7):991-996.
[11]马晓建,张霞,常春.丙酮丁醇梭菌发酵玉米秸秆生产丁醇[J].华南理工大学学报:自然科学版,2014,24(2):27-32.MA X J,ZHANG X,CHANG C.Butanol production from corn stover by fermentation with Clostridium acetobutylicum[J].Journal of South China University of Technology:Natural Science Edition,2014,24(2):27-32.
[12]王风芹,仝银杏,李传斌,等.蒸汽爆破玉米芯水解液脱毒及其发酵生产燃料丁醇[J].农业工程学报,2016,32(5):257-262.WANG F Q,TONG Y X,LI C B,et al.Hydrolysate detoxified from steam exploded corn cob and its fermentationproducing butanol fuels[J].Transactions of the Chinese Society of Agricultural Engineering,2016,32(5):257-262.
[13]龙飞,靳艳玲,赵云,等.丙酮丁醇梭菌Clostridium acetobutylicum CICC 8012发酵鲜芭蕉芋生产丁醇[J].应用与环境生物学报,2013,19(1):54-60.LONG F,JIN Y L,ZHAO Y,et al.Optimization of butanol production from Canna edulis Ker by Clostridium acetobutylicum using response surface methodology[J].Chinese Journal of Applied and Environmental Biology,2013,19(1):54-60.
[14]田毅红,朱志豪,高媛,等.红薯发酵产丁醇的工艺优化[J].化学与生物工程,2016,33(2):64-66.TIAN Y H,ZHU Z H,GAO Y,et al.Optimization on fermentation conditions of butanol from sweet potato[J].Chemistry&Bioengineering,2016,33(2):64-66.
[15]蒋玉峰,马代夫.国家甘薯产业技术体系建设推动甘薯产业和学科发展[J].江苏师范大学学报:自然科学版,2016,34(3):23-27.JIANG Y F,MA D F.The sweet potato of China agricultural research system promotes the industrilization and disciplinary development of sweet potato[J].Journal of Jiangsu Normal University:Natural Science Edition,2016,34(3):23-27.
[16]国家发展和改革委员会.可再生能源中长期发展规划[J].可再生能源,2007,25(6):1-5.National Development and Reform Commission.Longterm development plan for renewable energy[J].Renewable Energy Resources,2007,25(6):1-5.