黄皮白肉火龙果快繁体系的建立
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摘要
黄皮白肉火龙果,又名麒麟果,为建立其快繁体系,本研究选取种子萌发长出的茎段为外植体,研究其不同消毒时间、不同浓度激素配比对其愈伤组织及芽的诱导、增殖继代、根生长的影响。结果表明:用无水乙醇灭菌40 s后,再用5%次氯酸钠溶液灭菌10 min,种子萌发率最高可达97%。愈伤组织及不定芽诱导培养基最佳配方为1/2 MS+2.0 mg/L 6-BA+1.0 mg/L NAA,芽的诱导率最高可达97%;利用MS+2.0 mg/L 6-BA+3.0 mg/L NAA培养基进行增殖、继代培养的效果最佳,增值系数最高为4.6;在MS培养基中添加2.0 mg/L IBA,可最大程度地促进根的生长。
Selenicereus megalanthus is also known as yellow pitahaya. In order to establish a system of Selenicereus megalanthus tissue culture and rapid propagation, seed germination stem segments were used as the explants, and the effects of different disinfection time, different concentrations of hormones were studied on the callus germination, propagation, and the growth of the root. The result indicated that the germination rate was 97% by sterilization with pure ethanol in 40 s, 5% hypochlorite in 10 min. The suitable medium for callus germination was 1/2 MS+2.0 mg/L 6-BA+1.0 mg/L NAA, and the germination rate was 97%; The best medium for proliferation was MS+2.0 mg/L 6-BA+3.0 mg/L NAA, and the proliferation coefficient was 4.6; The best medium for rooting was MS+2.0 mg/L IBA.
Selenicereus megalanthus is also known as yellow pitahaya. In order to establish a system of Selenicereus megalanthus tissue culture and rapid propagation, seed germination stem segments were used as the explants, and the effects of different disinfection time, different concentrations of hormones were studied on the callus germination, propagation, and the growth of the root. The result indicated that the germination rate was 97% by sterilization with pure ethanol in 40 s, 5% hypochlorite in 10 min. The suitable medium for callus germination was 1/2 MS+2.0 mg/L 6-BA+1.0 mg/L NAA, and the germination rate was 97%; The best medium for proliferation was MS+2.0 mg/L 6-BA+3.0 mg/L NAA, and the proliferation coefficient was 4.6; The best medium for rooting was MS+2.0 mg/L IBA.
引文
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[16]洪青梅,胡文斌,李洪立,等.‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导[J].热带作物学报,2017,38(11):2 119-2 123.
[17]彭绿春,瞿素萍,苏艳,等.火龙果两步法组培苗快繁技术研究[J].西南农业学报.2014,27(6):2 529-2 533.
[18]张领,赵左敏,陈红艳,等,火龙果离体快繁与组培苗移植驯化技术[J].贵州农业科学.2016,44(2):20-23.
[19]葛宇,魏卿,刘永霞.外源GA3处理对黄皮白肉火龙果红衣发芽及幼苗鲜重的影响[J].中国南方果树,2016,45(5):73-75.
[20]葛宇,丁哲利,程志号,等.3种火龙果种子萌发及其幼苗形态的比较研究[J].种子,2017,36(7):22-24.
[21]黄春华,杨健荣,梁秋玲,等.‘蜜宝’火龙果的组织培养技术[J].热带农业工程,2013,37(3):31-33.
[2]高国丽,张冰雪,乔光,等.火龙果种质资源的耐寒性综合评价[J].华中农业大学学报2014,33(3):26-32.
[3]邓仁菊,范建新,王彬,等.火龙果抗寒性检测初探[J].植物生理学通讯,2009,45(10):1 023-1 026.
[4]Lichtenzveig J,Abbo S,Nerd A,et al.Cytology and mating systems in the climbing cacti Hylocereus and Selenicereus[J].American Journal of Botany,2000,87(7):1 058-1 065.
[5]陈杰,庞江琳,李尚德,等.火龙果的微量元素含量分析[J].广东微量元素科学,2004,11(5):56-57.
[6]Jamiilah B,Shu C E,Kharidah M.Physico-chemical characteristics of red pitaya(Hylocereus polyrhizus)peel[J].International Food Research Journal,2011,18(2):279-286.
[7]邓仁菊,范建新,蔡永强.国内外火龙果研究进展及产业发展现状[J].贵州农业科学,2011,39(6):188-192.
[8]范建新,邓仁菊,王永清,等.火龙果花药培养诱导胚性愈伤组织及遗传稳定性[J].分子植物育种,2017,15(1):258-266
[9]刘晓翠,文晓鹏,夏道强,等.贵州优异白肉火龙果B7的离题快繁技术研究[J].种子生产,2017,1(36):121-124
[10]谢志亮,吴振旺,增光辉.白玉龙火龙果成熟种子无菌萌发及组培技术研究[J].中国南方果树,2016,45(1):58-61.
[11]郑思成,文晓鹏.火龙果种胚的无菌快速催芽技术[J].种子,2011,30(7):76-78.
[12]Hao Y J,Deng X X.Cytological and molecular evaluation of strawberry plants recovered from in vitro conservation by slowgrowth[J].Jouranl of Horticultural Science&Biotechnology,2005,80(5):588-592.
[13]邹娜,陈璋,林思祖,等.福建山樱花愈伤组织的诱导及植株再生[J].核农学报,2013,27(10):1 417-1 423.
[14]Mohamed Y Y.Micropropagation of pitaya(Hylocereusundatus britt on et rose)[J].In Vitro Cellular&Developmental Biology-Plant,2002,38(5):427-429.
[15]王云山,周美虹,康黎芳,等.火龙果茎段组织培养快繁技术研究[J].山西农业科学,2012,40(5):455-458.
[16]洪青梅,胡文斌,李洪立,等.‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导[J].热带作物学报,2017,38(11):2 119-2 123.
[17]彭绿春,瞿素萍,苏艳,等.火龙果两步法组培苗快繁技术研究[J].西南农业学报.2014,27(6):2 529-2 533.
[18]张领,赵左敏,陈红艳,等,火龙果离体快繁与组培苗移植驯化技术[J].贵州农业科学.2016,44(2):20-23.
[19]葛宇,魏卿,刘永霞.外源GA3处理对黄皮白肉火龙果红衣发芽及幼苗鲜重的影响[J].中国南方果树,2016,45(5):73-75.
[20]葛宇,丁哲利,程志号,等.3种火龙果种子萌发及其幼苗形态的比较研究[J].种子,2017,36(7):22-24.
[21]黄春华,杨健荣,梁秋玲,等.‘蜜宝’火龙果的组织培养技术[J].热带农业工程,2013,37(3):31-33.