木薯‘新选048’二倍体及其同源四倍体对干旱胁迫的生理响应
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摘要
为研究不同倍性木薯对干旱胁迫耐受能力的差异,本研究以木薯品种‘新选048’二倍体及其同源四倍体盆栽苗为材料,设置不同程度的干旱胁迫,研究干旱对2个倍性木薯苗形态、渗透调节物质、抗氧化能力及内源激素的影响。结果表明:随着干旱胁迫程度的加强,2个倍性的木薯叶片均出现下垂、萎蔫、变黄,进而干枯的症状,二倍体表现更为严重。可溶性蛋白和脯氨酸(Pro)含量基本随着胁迫程度的加剧而逐渐增加,二倍体更为敏感,积累量大于四倍体。2个倍性材料的抗氧化相关指标基本随着干旱程度的加强而升高,四倍体总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)活性比二倍体高,而过氧化氢(H_2O_2)含量、丙二醛(MDA)含量、过氧化物酶(POD)活性和过氧化氢酶(CAT)活性比二倍体低。随着干旱程度的增加,2个倍性材料的赤霉素(GA)含量先上升后下降,在四倍体中的含量始终大于二倍体;吲哚乙酸(IAA)含量变化不规则;玉米素核苷(ZR)含量在二倍体中逐渐下降,而在四倍体中先轻微上升后下降,在四倍体中下降幅度较大,但ZR含量在四倍体中始终大于二倍体中的含量;脱落酸(ABA)含量先上升后下降,轻度胁迫下四倍体大于二倍体,其他程度胁迫下二倍体大于四倍体。综合分析,在干旱胁迫下,四倍体木薯产生较强的抗氧化能力,同时通过GA、ZR和ABA的协同作用,延长叶片的功能期,从而达到比二倍体抗旱的效果。
In order to analyse the different response to drought tolerance of cassava(Manihot esculenta) variety ‘Xinxuan 048' diploid and autotetraploid,we set up different treatments of drought stress to measure the morphology,osmotic adjustment indexes,antioxidant capacity and endogenous hormones of cassava diploid and its autotetraploid seedlings.The results showed that,with the increase of drought stress,leaves of the two genotype cassava appeared drooping,wilting,yellowing and withering,and the performance of diploid plant was more serious.Both of the contents of soluble protein and proline(Pro) of the two genotypes increased gradually with the increase of drought stress.The diploid was more sensitive,and accumulated more soluble protein and proline than the autotetraploid.The antioxidant indexes of the two genotype cassava increased with the strengthening of drought stress.The autotetraploid plant had stronger total antioxidant capacity(T-AOC) and higher superoxide dismutase(SOD) activity,but lower hydrogen peroxide(H_2O_2) content,malondialdehyde(MDA) content,peroxidase(POD) activity and catalase(CAT) activity compared with the diploid.With the increase of drought stress,the gibberellin(GA) contents in the two genotype cassava increased firstly and then drop.the GA content in autotetraploid plant was higher than that in the diploid.The indole acetic acid(IAA) contents of the two genotypes changed irregularity.The content of atin riboside(ZR) gradually declined in the diploid plant,but slightly increased firstly and then decreased quickly in autotetraploid,the ZR content in autotetraploid was always higher than that in diploid.It was observed that the content of abscisic acid(ABA) increased firstly and then decreased in both of the two genotype cassava,the autotetraploid ABA content under mild stress was great than that in diploid,but the other degree under stress was opposite.Comprehensive analysis showed that the autotetraploid cassava had strong antioxidant capacity under drought stress.Meanwhile,the synergistic effect of GA,ZR and ABA prolonged the functional period of leaves,so as to get higher drought resistance than diploid.
In order to analyse the different response to drought tolerance of cassava(Manihot esculenta) variety ‘Xinxuan 048' diploid and autotetraploid,we set up different treatments of drought stress to measure the morphology,osmotic adjustment indexes,antioxidant capacity and endogenous hormones of cassava diploid and its autotetraploid seedlings.The results showed that,with the increase of drought stress,leaves of the two genotype cassava appeared drooping,wilting,yellowing and withering,and the performance of diploid plant was more serious.Both of the contents of soluble protein and proline(Pro) of the two genotypes increased gradually with the increase of drought stress.The diploid was more sensitive,and accumulated more soluble protein and proline than the autotetraploid.The antioxidant indexes of the two genotype cassava increased with the strengthening of drought stress.The autotetraploid plant had stronger total antioxidant capacity(T-AOC) and higher superoxide dismutase(SOD) activity,but lower hydrogen peroxide(H_2O_2) content,malondialdehyde(MDA) content,peroxidase(POD) activity and catalase(CAT) activity compared with the diploid.With the increase of drought stress,the gibberellin(GA) contents in the two genotype cassava increased firstly and then drop.the GA content in autotetraploid plant was higher than that in the diploid.The indole acetic acid(IAA) contents of the two genotypes changed irregularity.The content of atin riboside(ZR) gradually declined in the diploid plant,but slightly increased firstly and then decreased quickly in autotetraploid,the ZR content in autotetraploid was always higher than that in diploid.It was observed that the content of abscisic acid(ABA) increased firstly and then decreased in both of the two genotype cassava,the autotetraploid ABA content under mild stress was great than that in diploid,but the other degree under stress was opposite.Comprehensive analysis showed that the autotetraploid cassava had strong antioxidant capacity under drought stress.Meanwhile,the synergistic effect of GA,ZR and ABA prolonged the functional period of leaves,so as to get higher drought resistance than diploid.
引文
An FF,Chen SB,Li GH,et al(2015).Comparison analysis of starch and protein expression profiles on cassava tuberous roots cv.SC8 and its tetraploid.Sci Agric Sin,48(13):2656-2665(in Chinese with English abstract)[安飞飞,陈松笔,李庚虎等(2015).华南8号木薯及其四倍体块根淀粉及蛋白表达谱的差异分析.中国农业科学,48(13):2656-2665]
An FF,Fan J,Li J,et al(2014).Comparison of leaf proteomes of cassava(Manihot esculenta Crantz)cultivar NZ199diploid and autotetraploid genotypes.PLo S ONE,9(4):e85991
Gao DH,Gao Q,Xu HY,et al(2009).Physiological responses to gradual drought stress in the diploid hybrid Pinus densata and its two parental species.Trees-struct Funct,23(4):717-728
Gao TM,Wu Y,Li F,et al(2017).Effects of water stress on physiological characteristics and growth under water stress in seedling of sesame.J Nucl Agric Sci,31(11):2229-2235(in Chinese with English abstract)[高桐梅,吴寅,李丰等(2017).苗期水分胁迫对芝麻生长和生理特性的影响.核农学报,31(11):2229-2235]
Ge YJ,He XY,Wang JF,et al(2014).Physiological and biochemical responses of Phoebe bournei seedlings to water stress and recovery.Acta Physiol Plant,36(5):1241-1250
Gu B,Yao QQ,Li KM,et al(2013).Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors.Starch-St?rke,65(3-4):253-263
Jia RF,Yang ZJ,Xu DP,et al(2013).Growth and endogenous hormone content of Dalbergia odorifera seedlings under drought stress.Ecol Environ,22(7):1136-1140(in Chinese with English abstract)[贾瑞丰,杨曾奖,徐大平等(2013).干旱胁迫对降香黄檀幼苗生长及内源激素含量的影响.生态环境学报,22(7):1136-1140]
Lai HJ(2014).Research on induction of cassava polyploid through 2n gametes(dissertation).Haikou:Hainan University(in Chinese with English abstract)[赖杭佳(2014).木薯2n配子途径诱导多倍体的研究(学位论文).海口:海南大学]
Lei Y(2008).Physiological responses of Populus przewalskii to oxidative burst caused by drought stress.Russ J Plant Physl,55(6):857
Li YP,Huang J,Xu RL,et al(2013).Effects of salicylic acid and brassinolide on physiological characteristics in cassava seedling under drought stress.Chin J Trop Agric,33(10):6-11(in Chinese with English abstract)[李一萍,黄洁,许瑞丽等(2013).干旱胁迫下水杨酸和油菜素内酯对木薯苗生理特性的影响.热带农业科学,33(10):6-11]
Lu SY,Chen CH,Wang ZH,et al(2009).Physiological responses of somaclonal variants of triploid bermudagrass(Cynodon transvaalensis×Cynodon dactylon)to drought stress.Plant Cell Rep,28:517-526
Ma WT(2007).The drought resistance of different citrus rootstock seedlings(dissertation).Guiyang:Guizhou University(in Chinese with English abstract)[马文涛(2007).不同柑橘实生砧木的抗旱性(学位论文).贵阳:贵州大学]
Maherali H,Walden AE,Husband BC(2009).Genome duplication and the evolution of physiological responses to water stress.New Phytol,184:721-731
Manzaneda AJ,Rey PJ,Bastida JM,et al(2012).Environmental aridity is associated with cytotype segregation and polyploidy occurrence in Brachypodium distachyon(Poaceae).New Phytol,193(3):797
Nie YM,Wen F,Guo WW(2013).Colchicine-induced autotetraploids of five cassava cultivars.J Huazhong Agric Univ,32(6):8-12(in Chinese with English abstract)[聂扬眉,文峰,郭文武(2013).秋水仙素诱导获得5个木薯品种的同源四倍体植株.华中农业大学学报,32(6):8-12]
Shan ZY,Luo XL,Fan WJ,et al(2015).Effects of drought stress on the physiological characteristics of cassava seeding.Chin J Trop Crop,36(2):339-343(in Chinese with English abstract)[单忠英,罗兴录,樊吴静等(2015).干旱胁迫对木薯苗生理特性影响研究.热带作物学报,36(2):339-343]
Song HY,Zhang ZW,An FF,et al(2014).Analysis on chlorophyll fluorescence parameters of photosystem II in cassava diploid and its autotetraploid.Chin J Trop Crop,35(12):2410-2413(in Chinese with English abstract)[宋红艳,张振文,安飞飞等(2014).华南205木薯及其同源四倍体PSII叶绿素荧光参数差异分析.热带作物学报,35(12):2410-2413]
Sun CJ,Cheng ZH,Sun PG,et al(2016).Research advances on polyploid breeding of tropical crops.China Trop Agric,(2):76-80(in Chinese with English abstract)[孙长君,程志号,孙佩光等(2016).热带作物多倍体育种研究进展.中国热带农业,(2):76-80]
Xie XY,Ma ZL,Bai P,et al(2014).The morphological and physiological responses of hot pepper(Capsicum annuum L.)to drought stress during blossom and fruit period.Acta Ecologica Sin,34(13):3797-3805(in Chinese with English abstract)[谢小玉,马仲炼,白鹏等(2014).辣椒开花结果期对干旱胁迫的形态与生理响应.生态学报,34(13):3797-3805]
Xu EK,Fan GQ,Niu SY,et al(2014).Transcriptome wide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa×Paulownia fortunei under drought stress.PLo S ONE,9(11):e113313
Zhang HY,Duan WX,Jie BT,et al(2018).Effects of drought stress at different growth stages on endogenous hormones and its relationship with storage root yield in sweetpotato.Acta Agron Sin,44(1):126-136(in Chinese with English abstract)[张海燕,段文学,解备涛等(2018).不同时期干旱胁迫对甘薯内源激素的影响及其与块根产量的关系.作物学报,44(1):126-136]
Zhang MD,Chen Q,Shen SH(2011).Physiological responses of two Jerusalem artichoke cultivars to drought stress induced by polyethylene glycol.Acta Physiol Plant,33:313-318
Zhang MS,Tan F,Zhang QT(2001).Physiological indices for rapid identification of sweet potato drought resistance and selection of methods.Sci Agric Sin,34(3):260-265(in Chinese with English abstract)[张明生,谈锋,张启堂(2001).快速鉴定甘薯品种抗旱性的生理指标及方法的筛选.中国农业科学,34(3):260-265]
Zhang P,An D,Ma QX,et al(2013).Key scientific questions and recent advances in cassava molecular breeding.SCIESIN Vitae,43(12):1082-1089(in Chinese with English abstract)[张鹏,安冬,马秋香等(2013).木薯分子育种中若干基本科学问题的思考与研究进展.中国科学生命科学,43(12):1082-1089]
Zhang SN,Zhang HL,Zhang Y,et al(2011).Studies on activities of antioxidant enzymes in diploid and tetraploid radish under drought stress.Acta Agric Jiangxi,23(4):39-41(in Chinese with English abstract)[张蜀宁,张红亮,张宇等(2011).干旱胁迫下二倍体、四倍体萝卜抗氧化酶活性比较.江西农业学报,23(4):39-41]
Zhao CJ,Kang SJ,Wang JH,et al(2000).Hormone regulation mechanism on wheat leaf aging.Acta Agric Boreali Sin,15(2):53-56(in Chinese with English abstract)[赵春江,康书江,王纪华等(2000).植物内源激素对小麦叶片衰老的调控机理研究.华北农学报,15(2):53-56]
Zhao WK,Tong JH,Xie SX,et al(2008).The effects of drought stress on endogenous phytohormones in citrange.Prog Modern Biomed,8(9):1662-1664(in Chinese with English abstract)[赵文魁,童建华,谢深喜等(2008).干旱胁迫对枳橙内源激素含量的影响.现代生物医学进展,8(9):1662-1664]
Zhou F,Liu ES,Zhao PJ,et al(2013).Impacts of drought stress on content of endogenous phytohormones at seedling stage of cassava.Agric Res Arid Areas,31(5):238-244(in Chinese with English abstract)[周芳,刘恩世,赵平娟等(2013).干旱胁迫对苗期木薯内源激素含量的影响.干旱地区农业研究,31(5):238-244]
Zhou HW(2016).Studies on tetraploid induction of cassava in vitro and evaluation of the mutagenic material(dissertation).Nanning:Guangxi University(in Chinese with English abstract)[周慧文(2016).木薯四倍体离体诱导与诱变材料评价研究(学位论文).南宁:广西大学]
Zhou YQ,Bo QY(2011).Research on changing of endogenous hormones of fruit trees under drought stress.North Fruits,(3):1-4(in Chinese with English abstract)[周晏起,卜庆雁(2011).干旱胁迫下果树内源激素变化规律研究进展.北方果树,(3):1-4]
An FF,Fan J,Li J,et al(2014).Comparison of leaf proteomes of cassava(Manihot esculenta Crantz)cultivar NZ199diploid and autotetraploid genotypes.PLo S ONE,9(4):e85991
Gao DH,Gao Q,Xu HY,et al(2009).Physiological responses to gradual drought stress in the diploid hybrid Pinus densata and its two parental species.Trees-struct Funct,23(4):717-728
Gao TM,Wu Y,Li F,et al(2017).Effects of water stress on physiological characteristics and growth under water stress in seedling of sesame.J Nucl Agric Sci,31(11):2229-2235(in Chinese with English abstract)[高桐梅,吴寅,李丰等(2017).苗期水分胁迫对芝麻生长和生理特性的影响.核农学报,31(11):2229-2235]
Ge YJ,He XY,Wang JF,et al(2014).Physiological and biochemical responses of Phoebe bournei seedlings to water stress and recovery.Acta Physiol Plant,36(5):1241-1250
Gu B,Yao QQ,Li KM,et al(2013).Change in physicochemical traits of cassava roots and starches associated with genotypes and environmental factors.Starch-St?rke,65(3-4):253-263
Jia RF,Yang ZJ,Xu DP,et al(2013).Growth and endogenous hormone content of Dalbergia odorifera seedlings under drought stress.Ecol Environ,22(7):1136-1140(in Chinese with English abstract)[贾瑞丰,杨曾奖,徐大平等(2013).干旱胁迫对降香黄檀幼苗生长及内源激素含量的影响.生态环境学报,22(7):1136-1140]
Lai HJ(2014).Research on induction of cassava polyploid through 2n gametes(dissertation).Haikou:Hainan University(in Chinese with English abstract)[赖杭佳(2014).木薯2n配子途径诱导多倍体的研究(学位论文).海口:海南大学]
Lei Y(2008).Physiological responses of Populus przewalskii to oxidative burst caused by drought stress.Russ J Plant Physl,55(6):857
Li YP,Huang J,Xu RL,et al(2013).Effects of salicylic acid and brassinolide on physiological characteristics in cassava seedling under drought stress.Chin J Trop Agric,33(10):6-11(in Chinese with English abstract)[李一萍,黄洁,许瑞丽等(2013).干旱胁迫下水杨酸和油菜素内酯对木薯苗生理特性的影响.热带农业科学,33(10):6-11]
Lu SY,Chen CH,Wang ZH,et al(2009).Physiological responses of somaclonal variants of triploid bermudagrass(Cynodon transvaalensis×Cynodon dactylon)to drought stress.Plant Cell Rep,28:517-526
Ma WT(2007).The drought resistance of different citrus rootstock seedlings(dissertation).Guiyang:Guizhou University(in Chinese with English abstract)[马文涛(2007).不同柑橘实生砧木的抗旱性(学位论文).贵阳:贵州大学]
Maherali H,Walden AE,Husband BC(2009).Genome duplication and the evolution of physiological responses to water stress.New Phytol,184:721-731
Manzaneda AJ,Rey PJ,Bastida JM,et al(2012).Environmental aridity is associated with cytotype segregation and polyploidy occurrence in Brachypodium distachyon(Poaceae).New Phytol,193(3):797
Nie YM,Wen F,Guo WW(2013).Colchicine-induced autotetraploids of five cassava cultivars.J Huazhong Agric Univ,32(6):8-12(in Chinese with English abstract)[聂扬眉,文峰,郭文武(2013).秋水仙素诱导获得5个木薯品种的同源四倍体植株.华中农业大学学报,32(6):8-12]
Shan ZY,Luo XL,Fan WJ,et al(2015).Effects of drought stress on the physiological characteristics of cassava seeding.Chin J Trop Crop,36(2):339-343(in Chinese with English abstract)[单忠英,罗兴录,樊吴静等(2015).干旱胁迫对木薯苗生理特性影响研究.热带作物学报,36(2):339-343]
Song HY,Zhang ZW,An FF,et al(2014).Analysis on chlorophyll fluorescence parameters of photosystem II in cassava diploid and its autotetraploid.Chin J Trop Crop,35(12):2410-2413(in Chinese with English abstract)[宋红艳,张振文,安飞飞等(2014).华南205木薯及其同源四倍体PSII叶绿素荧光参数差异分析.热带作物学报,35(12):2410-2413]
Sun CJ,Cheng ZH,Sun PG,et al(2016).Research advances on polyploid breeding of tropical crops.China Trop Agric,(2):76-80(in Chinese with English abstract)[孙长君,程志号,孙佩光等(2016).热带作物多倍体育种研究进展.中国热带农业,(2):76-80]
Xie XY,Ma ZL,Bai P,et al(2014).The morphological and physiological responses of hot pepper(Capsicum annuum L.)to drought stress during blossom and fruit period.Acta Ecologica Sin,34(13):3797-3805(in Chinese with English abstract)[谢小玉,马仲炼,白鹏等(2014).辣椒开花结果期对干旱胁迫的形态与生理响应.生态学报,34(13):3797-3805]
Xu EK,Fan GQ,Niu SY,et al(2014).Transcriptome wide profiling and expression analysis of diploid and autotetraploid Paulownia tomentosa×Paulownia fortunei under drought stress.PLo S ONE,9(11):e113313
Zhang HY,Duan WX,Jie BT,et al(2018).Effects of drought stress at different growth stages on endogenous hormones and its relationship with storage root yield in sweetpotato.Acta Agron Sin,44(1):126-136(in Chinese with English abstract)[张海燕,段文学,解备涛等(2018).不同时期干旱胁迫对甘薯内源激素的影响及其与块根产量的关系.作物学报,44(1):126-136]
Zhang MD,Chen Q,Shen SH(2011).Physiological responses of two Jerusalem artichoke cultivars to drought stress induced by polyethylene glycol.Acta Physiol Plant,33:313-318
Zhang MS,Tan F,Zhang QT(2001).Physiological indices for rapid identification of sweet potato drought resistance and selection of methods.Sci Agric Sin,34(3):260-265(in Chinese with English abstract)[张明生,谈锋,张启堂(2001).快速鉴定甘薯品种抗旱性的生理指标及方法的筛选.中国农业科学,34(3):260-265]
Zhang P,An D,Ma QX,et al(2013).Key scientific questions and recent advances in cassava molecular breeding.SCIESIN Vitae,43(12):1082-1089(in Chinese with English abstract)[张鹏,安冬,马秋香等(2013).木薯分子育种中若干基本科学问题的思考与研究进展.中国科学生命科学,43(12):1082-1089]
Zhang SN,Zhang HL,Zhang Y,et al(2011).Studies on activities of antioxidant enzymes in diploid and tetraploid radish under drought stress.Acta Agric Jiangxi,23(4):39-41(in Chinese with English abstract)[张蜀宁,张红亮,张宇等(2011).干旱胁迫下二倍体、四倍体萝卜抗氧化酶活性比较.江西农业学报,23(4):39-41]
Zhao CJ,Kang SJ,Wang JH,et al(2000).Hormone regulation mechanism on wheat leaf aging.Acta Agric Boreali Sin,15(2):53-56(in Chinese with English abstract)[赵春江,康书江,王纪华等(2000).植物内源激素对小麦叶片衰老的调控机理研究.华北农学报,15(2):53-56]
Zhao WK,Tong JH,Xie SX,et al(2008).The effects of drought stress on endogenous phytohormones in citrange.Prog Modern Biomed,8(9):1662-1664(in Chinese with English abstract)[赵文魁,童建华,谢深喜等(2008).干旱胁迫对枳橙内源激素含量的影响.现代生物医学进展,8(9):1662-1664]
Zhou F,Liu ES,Zhao PJ,et al(2013).Impacts of drought stress on content of endogenous phytohormones at seedling stage of cassava.Agric Res Arid Areas,31(5):238-244(in Chinese with English abstract)[周芳,刘恩世,赵平娟等(2013).干旱胁迫对苗期木薯内源激素含量的影响.干旱地区农业研究,31(5):238-244]
Zhou HW(2016).Studies on tetraploid induction of cassava in vitro and evaluation of the mutagenic material(dissertation).Nanning:Guangxi University(in Chinese with English abstract)[周慧文(2016).木薯四倍体离体诱导与诱变材料评价研究(学位论文).南宁:广西大学]
Zhou YQ,Bo QY(2011).Research on changing of endogenous hormones of fruit trees under drought stress.North Fruits,(3):1-4(in Chinese with English abstract)[周晏起,卜庆雁(2011).干旱胁迫下果树内源激素变化规律研究进展.北方果树,(3):1-4]