三江大蕉快速繁殖体系建立及优化
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摘要
三江大蕉(Musa spp. AAB Group cv. Plantain)是优异的海南地方品种,属于典型的酸蕉类型。文中以三江大蕉的吸芽为外植体,首次建立并优化了其快速繁殖体系。启动吸芽增殖的培养基为MS+4 mg/L 6-BA,启动率高达100%;吸芽增殖培养基为MS+3 mg/L 6-BA+0. 05 mg/L NAA,每代增殖效率为5. 76;生根培养基为1/2 MS+0. 05 mg/L NAA,生根迅速,生根率高达98%。通过建立并优化三江大蕉的快速繁殖体系,为三江大蕉的品种推广示范以及进一步的品种改良奠定了基础。
Sanjiang dajiao( Musa spp. AAB Group cv. Plantain) is an excellent Hainan banana cultivar,which belongs to the typical sour banana type. In this paper,the rapid mcro-propagation system of Sanjiang dajiao was established and optimized for the first time. MS medium with 4 mg/L 6-BA was used as sucks initiate proliferation medium,and the initiation rate was as high as 100%. MS medium with 3 mg/L 6-BA and 0. 05 mg/L NAA were used for sucks micro-propagation,and with 5. 76 multiplication efficiency per generation.The rooting medium was 1/2 MS medium with 0. 05 mg/L NAA,and the rooting rate was as high as 98%.This paper establishes and optimizes the rapid propagation system of Sanjiang dajiao,which lays a foundation for the extension and demonstration and the further improvement of Sanjiang dajiao.
Sanjiang dajiao( Musa spp. AAB Group cv. Plantain) is an excellent Hainan banana cultivar,which belongs to the typical sour banana type. In this paper,the rapid mcro-propagation system of Sanjiang dajiao was established and optimized for the first time. MS medium with 4 mg/L 6-BA was used as sucks initiate proliferation medium,and the initiation rate was as high as 100%. MS medium with 3 mg/L 6-BA and 0. 05 mg/L NAA were used for sucks micro-propagation,and with 5. 76 multiplication efficiency per generation.The rooting medium was 1/2 MS medium with 0. 05 mg/L NAA,and the rooting rate was as high as 98%.This paper establishes and optimizes the rapid propagation system of Sanjiang dajiao,which lays a foundation for the extension and demonstration and the further improvement of Sanjiang dajiao.
引文
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[3]孙长君,程志号,孙佩光,等.“三江大蕉”植物学性状观察及分类学地位[J].中国热带农业,2018(2):35~39.
[4] N. W. Simmonds,S. E. The taxonomy and origins of the cultivate bananas[J]. Linnean society of botany,1955(55):302~313.
[5]解辉.香蕉开放式组织培养研究[D].海口:海南大学硕士毕业论文,2011.
[6]李华平,胡晋生,王敏,等.香蕉茎尖遗传转化法研究[J].热带作物学报,2000,21(4):33~39.
[7]张建斌,贾彩红,刘菊华,等.香蕉未成熟雄花组织培养与快速繁殖研究[J].热带作物学报,2012,33(7):1125~1129.
[8]吴德邻.中国植物志[M].北京:科学出版社,1981.
[9]陈友,冯慧敏.海南野生蕉吸芽组培防褐化研究[J].热带农业工程,2012,36(6):12~16.
[10]韦家川,张慧英,郑比兰.多效唑和氯化胆碱对香蕉试管苗生长的影响[J].广西农业科学,1999,18(1):39~41.
[2]李锡文.云南芭蕉科植物[J].植物分类学报,1978,16(3):54~64.
[3]孙长君,程志号,孙佩光,等.“三江大蕉”植物学性状观察及分类学地位[J].中国热带农业,2018(2):35~39.
[4] N. W. Simmonds,S. E. The taxonomy and origins of the cultivate bananas[J]. Linnean society of botany,1955(55):302~313.
[5]解辉.香蕉开放式组织培养研究[D].海口:海南大学硕士毕业论文,2011.
[6]李华平,胡晋生,王敏,等.香蕉茎尖遗传转化法研究[J].热带作物学报,2000,21(4):33~39.
[7]张建斌,贾彩红,刘菊华,等.香蕉未成熟雄花组织培养与快速繁殖研究[J].热带作物学报,2012,33(7):1125~1129.
[8]吴德邻.中国植物志[M].北京:科学出版社,1981.
[9]陈友,冯慧敏.海南野生蕉吸芽组培防褐化研究[J].热带农业工程,2012,36(6):12~16.
[10]韦家川,张慧英,郑比兰.多效唑和氯化胆碱对香蕉试管苗生长的影响[J].广西农业科学,1999,18(1):39~41.