复合菌种固态发酵法提高甜高粱秸秆饲料品质的研究
|
摘要
试验探讨了不同菌种及菌种组合对固态发酵甜高粱秸秆酒糟生产蛋白饲料的影响。以产朊假丝酵母、康宁木霉、黑曲霉、枯草芽孢杆菌和白地霉为发酵初筛菌种,探索单一菌种及双菌组合对甜高粱秸秆酒糟木质纤维素、蛋白质含量积累的影响。结果表明,对比未发酵甜高粱秸秆酒糟数据可知,单一菌种和双菌组合均能提高发酵产物的蛋白质含量和降低粗纤维含量。黑曲霉和产朊假丝酵母组合为最佳菌种配伍,且当菌种比例为11时,粗蛋白质含量为18.79%;粗纤维含量为34.68%,较未接菌降低了16.38%,且较单一菌种黑曲霉和产朊假丝酵母分别降低了8.15%、7.01%。通过微生物菌种发酵处理可以降解部分纤维素、提高蛋白质含量,还可在很大程度上缓解饲料中蛋白不足的矛盾。
The effects of different strains and their combinations on the production of protein feed by solid state fermentation of sweet sorghum straw distiller's grains were studied. Candida utilis, Trichoderma koningii, Aspergillus niger, Bacillus subtilis and Geotrichum candidum were used as primary screening strains to explore the effects of single strains and double bacteria combinations on the accumulation of lignocellulose and protein content in sweet sorghum straw distiller's grains. The results showed that compared with the data of unfermented sweet sorghum straw distiller's grains, it can be seen that both single and double bacterial combinations can increase the protein content of the fermentation product and reduce the crude fiber content. The combination of Aspergillus niger and Candida utilis was the best compatibility, and when the proportion of strain was 11, the crude protein content was 18.79%. The crude fiber content was 34.68%, 16.38% lower than that without inoculation, and8.15% and 7.01% lower than that of Aspergillus niger and Candida utilis, respectively. Fermentation treatment with microbial strains can degrade part of cellulose and increase protein content, and can also largely alleviate the contradiction of protein deficiency in feed.
The effects of different strains and their combinations on the production of protein feed by solid state fermentation of sweet sorghum straw distiller's grains were studied. Candida utilis, Trichoderma koningii, Aspergillus niger, Bacillus subtilis and Geotrichum candidum were used as primary screening strains to explore the effects of single strains and double bacteria combinations on the accumulation of lignocellulose and protein content in sweet sorghum straw distiller's grains. The results showed that compared with the data of unfermented sweet sorghum straw distiller's grains, it can be seen that both single and double bacterial combinations can increase the protein content of the fermentation product and reduce the crude fiber content. The combination of Aspergillus niger and Candida utilis was the best compatibility, and when the proportion of strain was 11, the crude protein content was 18.79%. The crude fiber content was 34.68%, 16.38% lower than that without inoculation, and8.15% and 7.01% lower than that of Aspergillus niger and Candida utilis, respectively. Fermentation treatment with microbial strains can degrade part of cellulose and increase protein content, and can also largely alleviate the contradiction of protein deficiency in feed.
引文
[1]Qu H,Liu X B,Dong C F,et al.Field performance and nutritive value of sweet sorghum in eastern China[J].Field Crops Research,2014,157:84-88.
[2]Laopaiboon L,Nuanpeng S,Srinophakun P,et al.Ethanol production from sweet sorghum juice using very high gravity technology:effects of carbon and nitrogen supplementations[J].Bioresource technology,2009,100(18):4176-4182.
[3]赵凯,马龙彪,耿贵,等.能源作物甜高粱的综合开发与利用[J].中国糖料,2008(3):67-68.
[4]原海兵,刘军,孙东伟.复合菌种固态发酵豆粕生产蛋白饲料的研究[J].粮食与饲料工业,2013(6):46-50.
[5]Shen F,Saddler J N,Liu R,et al.Evaluation of steam pretreatment on sweet sorghum bagasse for enzymatic hydrolysis and bioethanol production[J].Carbohydrate polymers,2011,86(4):1542-1548.
[6]赵辰龙.利用甘蔗尾叶发酵生产蛋白饲料[D].广西大学,2013.
[7]杨森,王石垒,张雷,等.甜高粱茎秆残渣生料多菌种固态发酵生产蛋白饲料[J].农业工程学报,2015,31(15):309-314.
[8]杨耀刚.10株纤维素降解菌混菌的酶活特性研究[D].内蒙古农业大学,2018.
[9]Wang L,Luo Z,Shahbazi A.Optimization of simultaneous saccharification and fermentation for the production of ethanol from sweet sorghum(Sorghum bicolor)bagasse using response surface methodology[J].Industrial Crops and Products,2013,42:280-291.
[10]焦有宙,高赞,李刚,等.不同土著菌及其复合菌对玉米秸秆降解的影响[J].农业工程学报,2015(23):201-207.
[11]李明轩.室温条件下混菌发酵稻草秸秆的降解研究[D].延吉:延边大学,2012.
[12]徐雯.利用纤维素降解菌固态发酵提高粗饲料营养价值的研究[D].广西大学,2015.
[13]Pongsak M S.黑曲霉和绿色木霉处理棕榈树干产酶的研究[D].哈尔滨工业大学,2016.
[14]张文佳.产朊假丝酵母和白地霉混合固态发酵豆渣生产反刍动物饲料的研究[D].东北农业大学,2015.
[15]李国强.两种木霉混合菌产酶的条件优化及其对玉米秸秆降解的研究[D].东北林业大学,2011.
[16]张玉诚,薛白,达勒措,等.混菌固态发酵白酒糟开发为蛋白质饲料的条件优化及营养价值评定[J].动物营养学报,2016,28(11):3711-3720.
[17]张高波,李巨秀,来航线,等.菌种对苹果渣发酵饲料中蛋白酶活,纤维素酶活及总酚含量的影响[J].食品与发酵工业,2013,39(11):118-123.
[18]Wen Z,Liao W,Chen S.Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure[J].Process Biochemistry,2005,40(9):3087-3094.
[19]Juhasz T,Kozma K,Reczey K.Production ofβ-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30[J].Food technology and Biotechnology,2003,41(1):49-53.
[20]黎晔晖.复合菌种协同发酵降解木薯渣工艺研究[D].华南农业大学,2016.
[21]Marques N P,De Cassia Pereira J,Gomes E,et al.Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse[J].Industrial Crops and Products,2018,122:66-75.
[22]Suto M,Tomita F.Induction and catabolite repression mechanisms of cellulase in fungi[J].Journal of bioscience and bioengineering,2001,92(4):305-311.
[2]Laopaiboon L,Nuanpeng S,Srinophakun P,et al.Ethanol production from sweet sorghum juice using very high gravity technology:effects of carbon and nitrogen supplementations[J].Bioresource technology,2009,100(18):4176-4182.
[3]赵凯,马龙彪,耿贵,等.能源作物甜高粱的综合开发与利用[J].中国糖料,2008(3):67-68.
[4]原海兵,刘军,孙东伟.复合菌种固态发酵豆粕生产蛋白饲料的研究[J].粮食与饲料工业,2013(6):46-50.
[5]Shen F,Saddler J N,Liu R,et al.Evaluation of steam pretreatment on sweet sorghum bagasse for enzymatic hydrolysis and bioethanol production[J].Carbohydrate polymers,2011,86(4):1542-1548.
[6]赵辰龙.利用甘蔗尾叶发酵生产蛋白饲料[D].广西大学,2013.
[7]杨森,王石垒,张雷,等.甜高粱茎秆残渣生料多菌种固态发酵生产蛋白饲料[J].农业工程学报,2015,31(15):309-314.
[8]杨耀刚.10株纤维素降解菌混菌的酶活特性研究[D].内蒙古农业大学,2018.
[9]Wang L,Luo Z,Shahbazi A.Optimization of simultaneous saccharification and fermentation for the production of ethanol from sweet sorghum(Sorghum bicolor)bagasse using response surface methodology[J].Industrial Crops and Products,2013,42:280-291.
[10]焦有宙,高赞,李刚,等.不同土著菌及其复合菌对玉米秸秆降解的影响[J].农业工程学报,2015(23):201-207.
[11]李明轩.室温条件下混菌发酵稻草秸秆的降解研究[D].延吉:延边大学,2012.
[12]徐雯.利用纤维素降解菌固态发酵提高粗饲料营养价值的研究[D].广西大学,2015.
[13]Pongsak M S.黑曲霉和绿色木霉处理棕榈树干产酶的研究[D].哈尔滨工业大学,2016.
[14]张文佳.产朊假丝酵母和白地霉混合固态发酵豆渣生产反刍动物饲料的研究[D].东北农业大学,2015.
[15]李国强.两种木霉混合菌产酶的条件优化及其对玉米秸秆降解的研究[D].东北林业大学,2011.
[16]张玉诚,薛白,达勒措,等.混菌固态发酵白酒糟开发为蛋白质饲料的条件优化及营养价值评定[J].动物营养学报,2016,28(11):3711-3720.
[17]张高波,李巨秀,来航线,等.菌种对苹果渣发酵饲料中蛋白酶活,纤维素酶活及总酚含量的影响[J].食品与发酵工业,2013,39(11):118-123.
[18]Wen Z,Liao W,Chen S.Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure[J].Process Biochemistry,2005,40(9):3087-3094.
[19]Juhasz T,Kozma K,Reczey K.Production ofβ-glucosidase in mixed culture of Aspergillus niger BKMF 1305 and Trichoderma reesei RUT C30[J].Food technology and Biotechnology,2003,41(1):49-53.
[20]黎晔晖.复合菌种协同发酵降解木薯渣工艺研究[D].华南农业大学,2016.
[21]Marques N P,De Cassia Pereira J,Gomes E,et al.Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse[J].Industrial Crops and Products,2018,122:66-75.
[22]Suto M,Tomita F.Induction and catabolite repression mechanisms of cellulase in fungi[J].Journal of bioscience and bioengineering,2001,92(4):305-311.