铝胁迫下柠檬酸对多年生黑麦草种子萌发和幼苗生理特性的影响
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摘要
为探究柠檬酸处理对多年生黑麦草铝毒害的缓解作用,以多年生黑麦草品种维多利亚为试材,采用培养皿滤纸发芽法,研究柠檬酸不同浓度(0.2,0.5,0.8,1.1,1.5 mmol/L)对250 mg/L和500 mg/L浓度铝胁迫下多年生黑麦草种子萌发和幼苗特性的影响。结果表明:柠檬酸不能提高铝胁迫下多年生黑麦草的发芽势、发芽率及发芽指数,但能促进铝胁迫下其地上部分以及地下部分的生长,同时还能提高幼苗叶片SOD活性,使其MDA含量和相对电导率下降,柠檬酸浓度为0.8 mmol/L时对缓解铝胁迫对多年生黑麦草的毒害作用最适宜。
In order to investigate the mitigation effect of citric acid treatment on aluminum toxicity in perennial ryegrass,perennial ryegrass ‘Victoria' was used as a test material to study the seed germination and seedling characteristics treated with different concentrations(0.2,0.5,0.8,1.1,1.5 mmol/L) of citric acid under 250 mg/L and 500 mg/L of aluminum stress by using the culture dish filter paper germination method.The results showed that citric acid could not increase the germination potential,germination rate and germination index under aluminum stress,but it could promote the growth and SOD activity,decrease MDA content and relative conductivity of seedling leaf.In general,the addition of exogenous citric acid could alleviate the toxicity of aluminum stress on perennial ryegrass to a certain extent.
In order to investigate the mitigation effect of citric acid treatment on aluminum toxicity in perennial ryegrass,perennial ryegrass ‘Victoria' was used as a test material to study the seed germination and seedling characteristics treated with different concentrations(0.2,0.5,0.8,1.1,1.5 mmol/L) of citric acid under 250 mg/L and 500 mg/L of aluminum stress by using the culture dish filter paper germination method.The results showed that citric acid could not increase the germination potential,germination rate and germination index under aluminum stress,but it could promote the growth and SOD activity,decrease MDA content and relative conductivity of seedling leaf.In general,the addition of exogenous citric acid could alleviate the toxicity of aluminum stress on perennial ryegrass to a certain extent.
引文
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[2] 崔雪梅,简君萌,李春生.铝胁迫对油菜根系及叶片生理生化指标的影响[J].江苏农业科学,2015,43(12):107-109.
[3] 张争艳.大豆对铝胁迫响应的研究[D].金华:浙江师范大学,2008.
[4] 黄玉婷,吴亚,刘大林,等.铝胁迫对草本植物生理的影响机制[J].草业科学,2018,35(6):1517-1527.
[5] 黄凯丰,张兰,胡丽雪,等.不同柠檬酸处理对铝胁迫下苦荞幼苗生理特性的影响[J].广东农业科学,2012,39(11):28-29+33.
[6] 金婷婷,刘鹏,张志祥,等.外源柠檬酸缓解大豆根系短期铝胁迫的FTIR特征分析[J].光谱学与光谱分析,2009,29(2):367-371.
[7] 闫智臣,李应德,程维佳,等.不同盐浓度下AM真菌和禾草内生真菌对多年生黑麦草生长的影响[J].草原与草坪,2018,38(1):63-70.
[8] 陈鸣晖,刘大林,张卫红,等.铜污染土壤中不同多年生黑麦草品种富集效应的评价[J].草原与草坪,2017,37(6):78-83.
[9] 曾任森.化感作用研究中的生物测定方法综述[J].应用生态学报,1999,10(1):125-128.
[10] 刘萍,李明军.植物生理学实验技术[M].北京:科学出版社,2007.
[11] 陈爱葵,韩瑞宏,李东洋,等.植物叶片相对电导率测定方法比较研究[J].广东教育学院学报,2010,30(5):88-91.
[12] 宋超,王跃华,赵钢,等.不同酸处理对苦荞种子萌发和幼苗生长的影响[J].种子,2015,34(8):79-82.
[13] 于敏,王文国,王胜华,等.外源柠檬酸对水稻铜毒害的缓解效应[J].应用与环境生物学报,2010,16(5):617-621.
[14] 邢素芝,汪建飞,蔡丹.柠檬酸和苹果酸对小白菜种子萌发和细胞膜透性的影响[J].中国农学通报,2007(9):312-316.
[15] 王月平,章艺,吴玉环,等.植物铝毒害及抗铝毒机制[J].湖北农业科学,2011,50(19):3900-3903.
[16] 马志慧.铝胁迫下杉木无性系苗若干生理过程及转录组的研究[D].福州:福建农林大学,2015.
[17] 初晓辉,张艾青,段新慧,等.铝胁迫对多花黑麦草生长和生理的影响[J].草原与草坪,2017,37(6):48-56.
[18] 徐芬芬.柠檬酸对铝胁迫下大豆根系生长和生理特性的影响[J].生物加工过程,2015,13(4):75-78.
[19] 萧凤迴,沈振国,冯剑亚,等.钙和6-BA对绿豆幼苗耐铝性的影响[J].南京农业大学学报,1999,22(1):9-13.
[20] 孙远秀,邱爽,张伟伟,等.柠檬酸对西瓜幼苗铝毒害的缓解作用[J].核农学报,2016,30(10):2072-2079.
[21] Rengel Z,Robinson D L.Aluminum effects on growth and micronutrient uptake in annual ryegrass[J].Agronomy Journal,1989,81:208-215.
[22] 庄正,李艳娟,刘青青,等.外源低分子有机酸对杉木种子萌发及幼苗抗氧化特性的影响[J].江西农业大学学报,2017,39(2):302-311.
[23] Ma B H,Cao L,Zhang H X,et al.Aluminum-induced oxidative stress and changes in antioxidant defenses in the roots of rice varieties differing in Al tolerance[J].Plant Cell Reports,2012,31(4) :687-696.
[24] 贾松涛,郑阳霞,邱爽,等.铝胁迫对西瓜幼苗生长及生理特性的影响[J].农业环境科学学报,2014,33(8):1485-1492.
[25] 陈佳,韩杰,郑佳梦,等.外源柠檬酸对铝胁迫栝楼抗氧化酶系及根尖铝积累的影响[J].贵州农业科学,2018,46(2):120-123.
[26] 纪雨薇.马尾松铝胁迫生理响应机制[D].重庆:西南大学,2016.
[27] 曹林,吴玉环,章艺,等.外源水杨酸对铝胁迫下菊芋光合特性及耐铝性的影响[J].水土保持学报,2015,29(4):260-266.
[28] 龚良建,杨江山.水杨酸对设施延后栽培葡萄生理生化特性的影响[J].甘肃农业大学学报,2018,53(6):137-142.
[29] 郝磊,弓丽花,黄永芳.铝胁迫对3种油茶初生根生理特性的影响[J].贵州农业科学,2017,45(2):124-129.
[30] 欧阳玲.酸铝胁迫和土壤改良剂对白三叶生长的影响[D].长沙:湖南农业大学,2016.