贵州省食源性蜡样芽孢杆菌毒力基因及耐药研究
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摘要
目的了解贵州省食源性蜡样芽胞杆菌的毒力基因分布及耐药谱,为蜡样芽胞杆菌食源性疾病的防治提供参考依据。方法采用PCR方法对2015-2017年贵州省60株食源性蜡样芽胞杆菌进行10种毒力基因的检测;采用K-B法检测10种抗生素的敏感性。结果 60株蜡样芽胞杆菌共发现16种毒力基因携带模式,其中nheB、nheC检出率100%,nheA、entFM为96.67%(58/60),hblA为63.33%(38/60),hblC为56.67%(34/60),hblD为53.33%(33/60),bceT为15%(9/60),ces为3.33%(2/60)。60株蜡样芽胞杆菌对庆大霉素、氯霉素、环丙沙星、万古霉素敏感率100%,对四环素、红霉素、克林霉素、氨苄西林、甲氧苄啶的敏感率分别为98.33%、91.67%、86.67%、13.33%、3.33%,对头孢吡肟100%耐药。结论贵州省食源性蜡样芽胞杆菌毒素基因携带率较高,存在引起食源性疾病的风险。氨苄西林、甲氧苄啶、头孢吡肟不建议作为蜡样芽孢杆菌引起的食源性疾病的临床治疗用药。
Objective To understand the virulent gene profiles and antimicrobial resistance of foodborne Bacillus cereus in Guizhou province,and to provide reference for the control of foodborne diseases.Methods By using PCR method,10 virulent genes of 60 food-borne B.cereus strains in Guizhou province were detected.Antibiotic sensitivity of these strains was also detected using keyboard(K-B)method.Results A total of 16 toxigenic patterns were found in 60 B.cereus strains.The detection rates of gene nheB and nheC were 100%,while nheA and entFM were 96.67%.Detection rates of hblA,hblC,hblD,bceT and ces were 63.33%,56.67%,53.33%,15% and 3.33%,respectively.For gene cytK,detection rate was negligible.All B.cereus strains were sensitive to gentamicin,chloramphenicol,ciprofloxacin and vancomycin,but were resistant to cefepime.The sensitivity rates to tetracycline,erythromycin,clindamycin,ampicillin,and trimethoprim were 98.33%,91.67%,86.67%,13.33%,and 3.33%,respectively.Conclusion The food-borne B.cereus toxin gene carrying rate in Guizhou province is high,so there is a risk of foodborne diseases.Ampicillin,trimethoprim,and cefepime are not recommended as clinical treatments for foodborne diseases caused by B.cereus.
Objective To understand the virulent gene profiles and antimicrobial resistance of foodborne Bacillus cereus in Guizhou province,and to provide reference for the control of foodborne diseases.Methods By using PCR method,10 virulent genes of 60 food-borne B.cereus strains in Guizhou province were detected.Antibiotic sensitivity of these strains was also detected using keyboard(K-B)method.Results A total of 16 toxigenic patterns were found in 60 B.cereus strains.The detection rates of gene nheB and nheC were 100%,while nheA and entFM were 96.67%.Detection rates of hblA,hblC,hblD,bceT and ces were 63.33%,56.67%,53.33%,15% and 3.33%,respectively.For gene cytK,detection rate was negligible.All B.cereus strains were sensitive to gentamicin,chloramphenicol,ciprofloxacin and vancomycin,but were resistant to cefepime.The sensitivity rates to tetracycline,erythromycin,clindamycin,ampicillin,and trimethoprim were 98.33%,91.67%,86.67%,13.33%,and 3.33%,respectively.Conclusion The food-borne B.cereus toxin gene carrying rate in Guizhou province is high,so there is a risk of foodborne diseases.Ampicillin,trimethoprim,and cefepime are not recommended as clinical treatments for foodborne diseases caused by B.cereus.
引文
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[2] 庄子慧,何丽,郭云昌,等.我国食源性蜡样芽孢杆菌毒力基因和药物敏感性研究[J].中国食品卫生杂志,2013,25(3):198-201.
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[4] 李毅,章乐怡,吴可可,等.温州地区食源性蜡样芽孢杆菌生化分型、毒素和耐药性分析[J].中国卫生检验杂志,2014,24(20):3010-3012.
[5] 闫韶飞,闫旭,甘辛,等.我国市售婴儿配方乳粉中蜡样芽胞杆菌污染及其毒力基因调查[J].中国食品卫生杂志,2015,27(3):286-291.
[6] Wehrle E,Moravek M,Dietrich R,et al.Comparison of multiplex PCR,enzyme immunoassay and cell culture methods for the detection of entertoxinogenic Bacillus cereus[J].Journal of Microbiological Methods,2009,78(3):265-270.
[7] 秦丽云,吕国平,郭玉梅,等.石家庄市市售婴幼儿食品中蜡样芽胞杆菌的监测及溶血素基因的分析[J].中国食品卫生杂志,2014,26(4):388-390.
[8] 中华人民共和国卫生部.WS/T 82-1996 蜡样芽胞杆菌食物中毒诊断标准及处理原则[S].北京:中国标准出版社,1997.
[9] 倪刚,倪文玲,何美红,等.一起蜡样芽胞杆菌食物中毒分离株的毒力基因分析研究[J].现代预防医学,2016,43(13):2439-2441.
[10] 高景枝,郑向梅,王滨.一起由蜡样芽孢杆菌引起食物中毒调查分析[J].中国公共卫生管理,2008,24(4):387-388.
[11] 殷全喜,张秀红,杜欣,等.一起蜡样芽孢杆菌引起的食物中毒的调查报告[J].中国预防医学杂志,2006,7(2):115-117.
[12] Moravek M,Dietrich R,Buerk C,et al.Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses[J].FEMS Microbiology Letters,2006,257(2):293-298.
[13] 章乐怡,张秀尧,李毅,等.婴幼儿奶粉和米粉中蜡样芽胞杆菌及其毒素、毒力基因的调查研究[J].中国食品卫生杂志,2014,26(6):600-604.
[14] 郭贵海,王崇文.肠道菌群调节剂的研究进展[J].临床内科杂志,2002,19(2):88-90.
[15] 黄俊云,邓晓.208例蜡样芽胞杆菌的分离及耐药性分析[J].现代预防医学,2010,37(16):3140-3141,3149.
[16] 常虹,周汉洪,王维,等.达州市食源性蜡样芽胞杆菌药敏试验结果分析[J].中国卫生检验杂志,2018,28(6):724-726.
[17] 丁元廷.细菌耐药机制的国内外最新研究进展[J].现代预防医学,2013,40(6):1109-1111.
[2] 庄子慧,何丽,郭云昌,等.我国食源性蜡样芽孢杆菌毒力基因和药物敏感性研究[J].中国食品卫生杂志,2013,25(3):198-201.
[3] Heilkenbrinker U,Dietrich R,Didier A,et al.Complex formation between NheB and NheC is necessary to induce cytotoxic activity by the three-component Bacillus cereus Nhe enterotoxin[J].PLOS One,2013,8(4):e63104.
[4] 李毅,章乐怡,吴可可,等.温州地区食源性蜡样芽孢杆菌生化分型、毒素和耐药性分析[J].中国卫生检验杂志,2014,24(20):3010-3012.
[5] 闫韶飞,闫旭,甘辛,等.我国市售婴儿配方乳粉中蜡样芽胞杆菌污染及其毒力基因调查[J].中国食品卫生杂志,2015,27(3):286-291.
[6] Wehrle E,Moravek M,Dietrich R,et al.Comparison of multiplex PCR,enzyme immunoassay and cell culture methods for the detection of entertoxinogenic Bacillus cereus[J].Journal of Microbiological Methods,2009,78(3):265-270.
[7] 秦丽云,吕国平,郭玉梅,等.石家庄市市售婴幼儿食品中蜡样芽胞杆菌的监测及溶血素基因的分析[J].中国食品卫生杂志,2014,26(4):388-390.
[8] 中华人民共和国卫生部.WS/T 82-1996 蜡样芽胞杆菌食物中毒诊断标准及处理原则[S].北京:中国标准出版社,1997.
[9] 倪刚,倪文玲,何美红,等.一起蜡样芽胞杆菌食物中毒分离株的毒力基因分析研究[J].现代预防医学,2016,43(13):2439-2441.
[10] 高景枝,郑向梅,王滨.一起由蜡样芽孢杆菌引起食物中毒调查分析[J].中国公共卫生管理,2008,24(4):387-388.
[11] 殷全喜,张秀红,杜欣,等.一起蜡样芽孢杆菌引起的食物中毒的调查报告[J].中国预防医学杂志,2006,7(2):115-117.
[12] Moravek M,Dietrich R,Buerk C,et al.Determination of the toxic potential of Bacillus cereus isolates by quantitative enterotoxin analyses[J].FEMS Microbiology Letters,2006,257(2):293-298.
[13] 章乐怡,张秀尧,李毅,等.婴幼儿奶粉和米粉中蜡样芽胞杆菌及其毒素、毒力基因的调查研究[J].中国食品卫生杂志,2014,26(6):600-604.
[14] 郭贵海,王崇文.肠道菌群调节剂的研究进展[J].临床内科杂志,2002,19(2):88-90.
[15] 黄俊云,邓晓.208例蜡样芽胞杆菌的分离及耐药性分析[J].现代预防医学,2010,37(16):3140-3141,3149.
[16] 常虹,周汉洪,王维,等.达州市食源性蜡样芽胞杆菌药敏试验结果分析[J].中国卫生检验杂志,2018,28(6):724-726.
[17] 丁元廷.细菌耐药机制的国内外最新研究进展[J].现代预防医学,2013,40(6):1109-1111.