电针不同经穴对心肌缺血模型大鼠大脑皮质区神经生长因子、酪氨酸激酶A表达的影响
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摘要
目的:观察电针心经原穴"神门"、小肠经络穴"支正"和背俞穴"心俞"对心肌缺血大鼠大脑皮质内神经生长因子(NGF)及其受体酪氨酸激酶A(TrkA)表达的影响,比较针刺不同经穴抗心肌缺血效应的差异,探讨针刺经穴效应的相对特异性。方法:雄性SD大鼠随机选取10只为假手术对照组,其余70只采用左冠状动脉前降支结扎法复制急性心肌缺血模型,模型复制成功的大鼠采用随机数字表分为模型组、神门组、支正组和心俞组,每组15只。各电针组电针相应穴位,每天治疗1次,每次15min,共治疗7d。采用免疫组化法和实时荧光定量PCR技术检测各组大鼠大脑皮质中NGF及其受体TrkA的表达。结果:与假手术组比较,模型组大鼠大脑皮质NGF及其受体TrkA阳性细胞数以及NGF、TrkA mRNA的表达差异无统计学意义(P>0.05)。与模型组比较,各针刺治疗组的大脑皮质NGF及其受体TrkA阳性表达细胞数目和NGF、TrkA mRNA表达均有显著增加(P<0.01,P<0.05)。与支正组及神门组比较,心俞组大脑皮质NGF、TrkA阳性细胞数和NGF、TrkA mRNA表达增多(P<0.01,P<0.05)。结论:电针"神门""支正""心俞"均能够提高心肌缺血大鼠大脑皮质中NGF及其受体TrkA的表达水平,且"心俞"穴效应最佳,具有相对特异性。
Objective To compare the therapeutic effect of electroacupuncture(EA)of"Shenmen"(HT 7,Yuan point of the Heart Meridian),"Zhizheng"(SI 7,Luo point of the Small Intestine Meridian)and"Xinshu"(BL15,Back-shu point)on the expression of nerve growth factor(NGF)and its receptor,tyrosine kinase A(TrkA)in the cerebral cortex of myocardial ischemia(MI)rats.Methods A total of 70 male SD rats were randomized into sham operation(sham,n=10),model,HT 7,SI 7,and BL15 groups(n=15 in each of the latter 4 groups).The MI model was established by ligation of the anterior descending branch of the left coronary artery.EA(1 mA,2 Hz)was respectively applied to HT 7,SI 7 and BL15 for 15 min,once per day for 1 week.Immunohistochemistry and real-time fluorescent quantitative PCR were used to detect the expression of NGF and TrkA proteins and genes in the cerebral cortex.Results Compared with the sham group,no significant changes were found in the number of NGF immune-reaction(IR)-positive and TrkA IR-positive cells,and the expression levels of NGF mRNA and TrkA mRNA in the model group(P>0.05).After EA intervention,the number of NGF and TrkA IR-positive cells and the expression of NGF mRNA and TrkA mRNA in each of the 3 EA groups were significantly increased relevant to the model group(P<0.01,P<0.05).The effect of EA at BL 15 was significantly superior to that of EA at HT 7 and SI 7 in increasing the number of NGF and TrkA positive cells and up-regulating the expression of their mRNAs(P<0.01,P<0.05).Conclusion EA at HT 7,SI 7 and BL 15 can increase the levels of expression of NGF and TrkA proteins and mRNAs in the cerebral cortex of subacute MI rats and the effects of EA-BL 15 are obviously superior to those of EA-HT 7 and EA-SI 7,suggesting a relative specificity of the effect of EA at different acupoints.
Objective To compare the therapeutic effect of electroacupuncture(EA)of"Shenmen"(HT 7,Yuan point of the Heart Meridian),"Zhizheng"(SI 7,Luo point of the Small Intestine Meridian)and"Xinshu"(BL15,Back-shu point)on the expression of nerve growth factor(NGF)and its receptor,tyrosine kinase A(TrkA)in the cerebral cortex of myocardial ischemia(MI)rats.Methods A total of 70 male SD rats were randomized into sham operation(sham,n=10),model,HT 7,SI 7,and BL15 groups(n=15 in each of the latter 4 groups).The MI model was established by ligation of the anterior descending branch of the left coronary artery.EA(1 mA,2 Hz)was respectively applied to HT 7,SI 7 and BL15 for 15 min,once per day for 1 week.Immunohistochemistry and real-time fluorescent quantitative PCR were used to detect the expression of NGF and TrkA proteins and genes in the cerebral cortex.Results Compared with the sham group,no significant changes were found in the number of NGF immune-reaction(IR)-positive and TrkA IR-positive cells,and the expression levels of NGF mRNA and TrkA mRNA in the model group(P>0.05).After EA intervention,the number of NGF and TrkA IR-positive cells and the expression of NGF mRNA and TrkA mRNA in each of the 3 EA groups were significantly increased relevant to the model group(P<0.01,P<0.05).The effect of EA at BL 15 was significantly superior to that of EA at HT 7 and SI 7 in increasing the number of NGF and TrkA positive cells and up-regulating the expression of their mRNAs(P<0.01,P<0.05).Conclusion EA at HT 7,SI 7 and BL 15 can increase the levels of expression of NGF and TrkA proteins and mRNAs in the cerebral cortex of subacute MI rats and the effects of EA-BL 15 are obviously superior to those of EA-HT 7 and EA-SI 7,suggesting a relative specificity of the effect of EA at different acupoints.
引文
[1]青虎,王丽杰,郑国.冠心病治疗进展[J].内蒙古民族大学学报(自然科学版),2008,23(6):693-695.
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[6]胡玲,蔡荣林,吴子建,等.电针不同经穴对急性心肌缺血家兔心功能的影响[J].针刺研究,2008,33(2):88-92.
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[8]郭鹞.人类疾病的动物模型[M].北京:人民卫生出版社,1982:177-182.
[9]王月兰,刘婧,龙迪和,等.非人工呼吸下大鼠心肌缺血再灌注损伤模型的复制[J].中国中医急症,2008,17(7):969-970.
[10]陈业农,周逸平,汪克明,等.急性心肌缺血再灌注损伤大鼠模型制备方法的改进与评价[J].中国中医急症,2008,17(3):359-363.
[11]李忠仁.实验针灸学[M].2版,北京:中国中医药出版社,2010:255-257.
[12]徐莉,饶春明.神经生长因子的研究进展[J].中国生物制品学杂志,2014,27(1):131-134.
[13]吴剑涓.神经生长因子的药理作用及临床应用进展[J].中国药房,2009,20(35):2779-2782.
[14]李强,王冬阳,赵宝东.神经生长因子与脑缺血[J].锦州医学院学报,2004,25(2):52-54.
[15]刘芳,张伟玲,邹伟.神经生长因子对急性神经损伤保护作用机制初探[J].中国急救医学,2006,26(10):687-689.
[16]朱奕,王彤.神经生长因子的生物学效应及其在脑损伤康复中的研究进展[J].中国康复医学杂志,2005,20(6):474-476.
[17]胡玲.经络腧穴学[M].上海:上海科学技术出版社,2009:28-29.
[18]何璐,龙迪和,胡玲,等.电针不同经穴对急性心肌缺血家兔血浆乙酰胆碱与去甲肾上腺素的影响[J].中国医药导报,2008,5(23):23-25.
[19]蔡荣林,胡玲,吴子建,等.电针不同原穴对急性心肌缺血家兔心率变异性的影响[J].云南中医学院学报,2013,36(5):23-24.
[20]李梦.电针内关、心俞改善急性心肌缺血大鼠血流变参数的协同作用[J].甘肃中医学院学报,2008,25(2):12-15.
[21]吴子建,蔡荣林,何璐,等.针刺“内关”“神门”穴对高脂血症大鼠心肌梗死后室旁核区和血清5-羟色胺含量的影响[J].针刺研究,2013,38(6):482-487.
[22]余情,胡玲,吴子建,等.电针“神门”“太渊”穴对急性心肌缺血家兔听感觉门控电位P 50的影响[J].针刺研究,2014,39(6):472-476.
[23]王洁,胡玲,许静,等.电针不同穴组对心肌缺血大鼠海马脑源性神经营养因子、酪氨酸激酶B表达的影响[J].针刺研究,2016,41(1):40-44.
[2]BALLEGAARD S,BORG E,KARPATSCHOF B,et al.Longterm effects of integrated rehabilitation in patients with advanced angina pectoris:a nonrandomized comparative study[J].J Altern Complement Med,2004,10(5):777-783.
[3]ELLIS A,WISEMAN N,BOSS K.Fundamentals of Chinese Acupuncture[M].Paradigm Publications,1991.
[4]STUX G,HAMMERSCHLAG R.Clinical Acupuncture:Scientific Basis[M].Seattle:Springer-Verlag,2001.
[5]蔡荣林,胡玲,周逸平,等.电针对急性心肌缺血家兔心功能及心交感神经电活动的影响[J].针刺研究,2007,32(4):243-246.
[6]胡玲,蔡荣林,吴子建,等.电针不同经穴对急性心肌缺血家兔心功能的影响[J].针刺研究,2008,33(2):88-92.
[7]倪宗赞.卫生统计学[M].4版,北京:人民卫生出版社,2002:267.
[8]郭鹞.人类疾病的动物模型[M].北京:人民卫生出版社,1982:177-182.
[9]王月兰,刘婧,龙迪和,等.非人工呼吸下大鼠心肌缺血再灌注损伤模型的复制[J].中国中医急症,2008,17(7):969-970.
[10]陈业农,周逸平,汪克明,等.急性心肌缺血再灌注损伤大鼠模型制备方法的改进与评价[J].中国中医急症,2008,17(3):359-363.
[11]李忠仁.实验针灸学[M].2版,北京:中国中医药出版社,2010:255-257.
[12]徐莉,饶春明.神经生长因子的研究进展[J].中国生物制品学杂志,2014,27(1):131-134.
[13]吴剑涓.神经生长因子的药理作用及临床应用进展[J].中国药房,2009,20(35):2779-2782.
[14]李强,王冬阳,赵宝东.神经生长因子与脑缺血[J].锦州医学院学报,2004,25(2):52-54.
[15]刘芳,张伟玲,邹伟.神经生长因子对急性神经损伤保护作用机制初探[J].中国急救医学,2006,26(10):687-689.
[16]朱奕,王彤.神经生长因子的生物学效应及其在脑损伤康复中的研究进展[J].中国康复医学杂志,2005,20(6):474-476.
[17]胡玲.经络腧穴学[M].上海:上海科学技术出版社,2009:28-29.
[18]何璐,龙迪和,胡玲,等.电针不同经穴对急性心肌缺血家兔血浆乙酰胆碱与去甲肾上腺素的影响[J].中国医药导报,2008,5(23):23-25.
[19]蔡荣林,胡玲,吴子建,等.电针不同原穴对急性心肌缺血家兔心率变异性的影响[J].云南中医学院学报,2013,36(5):23-24.
[20]李梦.电针内关、心俞改善急性心肌缺血大鼠血流变参数的协同作用[J].甘肃中医学院学报,2008,25(2):12-15.
[21]吴子建,蔡荣林,何璐,等.针刺“内关”“神门”穴对高脂血症大鼠心肌梗死后室旁核区和血清5-羟色胺含量的影响[J].针刺研究,2013,38(6):482-487.
[22]余情,胡玲,吴子建,等.电针“神门”“太渊”穴对急性心肌缺血家兔听感觉门控电位P 50的影响[J].针刺研究,2014,39(6):472-476.
[23]王洁,胡玲,许静,等.电针不同穴组对心肌缺血大鼠海马脑源性神经营养因子、酪氨酸激酶B表达的影响[J].针刺研究,2016,41(1):40-44.