甜瓜芽黄标记性状的发现与遗传分析
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摘要
为了使甜瓜芽黄标记性状能够更好地应用于甜瓜育种工作,笔者利用在本单位甜瓜试验地发现的甜瓜芽黄突变材料,研究了甜瓜芽黄资源的遗传规律。通过进行单株自交留种,并与正常叶色材料配制正、反交组合,其F1代均表现为正常叶色;对正、反交F1代与芽黄性状植株进行测交和自交,并对其测交后代与F2代的正常叶色植株与芽黄植株分离结果进行统计分析,结果表明,测交分离后代中正常叶色植株与芽黄植株的比率均接近于1∶1,正、反交F2代中正常叶色植株与芽黄植株的比率均接近于3∶1,经X2c适合性测验,差异不显著;同时发现,甜瓜芽黄材料通过连续多代自交,其后代均表现为芽黄,说明甜瓜芽黄突变性状可稳定遗传,属1对隐性基因控制的细胞核遗传。
In order to make yellow markers in melon buds better applied in melon breeding, the genetic rules of the melon bud yellow resources were studied in this experiment. The melon bud yellow mutant material found in the melon test field was used for self-seeding of the single plant, and the normal leaf color material was formulated with positive and negative cross combinations. the F1 generation showed normal leaf color; make the F1 generation and the yellow-branch plants for test cross and selfing, the results of the separation of the normal leaf color and the yellow shoots of the test cross progeny and the F2 generation were statistically analyzed, the results showed that the separation test cross descendants in the normal leaves and shoots yellow color plants were close to the ratio of 1∶1, and the ratios of the normal leaves and shoots yellow color plants in F2 generation plants were close to 3∶1, the difference was not significant after the X2 c suitability test. Meanwhile, we found that the melon bud yellow material was self-crossed for several generation successively, and all its progeny showed yellow buds, indicating that the mutant character of melon bud yellow could be inherited stably,belonging to a pair of recessive gene-controlled nuclear genetics.
In order to make yellow markers in melon buds better applied in melon breeding, the genetic rules of the melon bud yellow resources were studied in this experiment. The melon bud yellow mutant material found in the melon test field was used for self-seeding of the single plant, and the normal leaf color material was formulated with positive and negative cross combinations. the F1 generation showed normal leaf color; make the F1 generation and the yellow-branch plants for test cross and selfing, the results of the separation of the normal leaf color and the yellow shoots of the test cross progeny and the F2 generation were statistically analyzed, the results showed that the separation test cross descendants in the normal leaves and shoots yellow color plants were close to the ratio of 1∶1, and the ratios of the normal leaves and shoots yellow color plants in F2 generation plants were close to 3∶1, the difference was not significant after the X2 c suitability test. Meanwhile, we found that the melon bud yellow material was self-crossed for several generation successively, and all its progeny showed yellow buds, indicating that the mutant character of melon bud yellow could be inherited stably,belonging to a pair of recessive gene-controlled nuclear genetics.
引文
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[10]邵勤,于泽源,李兴国,等.叶色黄化突变体甜瓜叶片内部生理生化变化的研究[J].中国蔬菜,2013(14):59-65.
[11]马志虎,孙国胜,孙春青,等.蔬菜芽黄突变体研究进展[J].中国瓜菜,2013(5):1-5.
[12]何冰,刘玲珑,张文伟,等.植物叶色突变体[J].植物生理学通讯,2006,42(1):1-9.
[2]马国荣,刘佑斌,盖钧镒.大豆细胞质遗传芽黄突变体的发现[J].作物学报,1994,20(3):334-338.
[3]马志虎,颜素芳,罗秀龙,等.辣椒黄绿苗突变体对良种繁育及纯度鉴定作用[J].北方园艺,2001(3):13-14.
[4] TERRY M J,KENDRICK R E.Feedback inhibition of chlorophyll synthesisin the phytochrome chromophore-deficient aurea and yellow-green-2 mutants of tomato[J].Plant Physiol,1999,119(1):143-152.
[5]王凤辰,王浩波.西瓜“芽黄”新突变体简报[J].中国西瓜甜瓜,1997,10(3):14-15.
[6]王聪田,王国槐,青先国,等.一个新的水稻黄化突变体的光合作用及叶绿素荧光特性研究[J].江西农业学报,2007,19(9):10-13.
[7] LUO PEIGAO,REN ZHENGLONG.Wheat leaf chlorosis controlledby a single recessive gene[J].Journal of Plant Physiology and Molecular Biology,2006,32(3):330-338.
[8]潘跃平,金永庆,戴忠良,等.甘蓝型油菜芽黄突变体特异种质的发现及遗传分析[J].江苏农业学报,2009,25(5):1183-1184.
[9]陈远良,刘新宇,李树贤.黄瓜芽黄突变体的发现及其遗传分析[J].中国蔬菜,2000(3):35-36.
[10]邵勤,于泽源,李兴国,等.叶色黄化突变体甜瓜叶片内部生理生化变化的研究[J].中国蔬菜,2013(14):59-65.
[11]马志虎,孙国胜,孙春青,等.蔬菜芽黄突变体研究进展[J].中国瓜菜,2013(5):1-5.
[12]何冰,刘玲珑,张文伟,等.植物叶色突变体[J].植物生理学通讯,2006,42(1):1-9.