不同窖泥功能菌液细菌群落的研究
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摘要
采用多聚酶链式反应结合变性梯度凝胶电泳指纹分析技术(PCR-DGGE)对己酸菌和液体窖泥混合菌液、窖泥复合功能菌液、窖泥特殊生香功能菌液中细菌群落结构进行分析,并对不同质量窖泥接种培养的窖泥功能菌液中细菌群落结构进行对比分析,从微生物物种多样性的角度说明了不同窖泥功能菌液细菌的复杂性以及不同窖泥功能菌液具有不同生理、生化功能的原因,表明窖泥的质量与窖泥功能菌液、窖泥特殊生香功能菌液的质量可能存在相关性,为窖泥功能菌培养的进一步改进提供了理论依据。
Polymerase chain reaction denaturing gradient gel electrophoresis(PCR-DGGE) was used to analyse the bacterial community structure of different microbial culture liquid,such as the mixed culture liquid of caproic acid bacterial and liquid pit mud, the compound culture liquid of functional microbial in pit mud and the culture liquid of special aroma producing bacteria in pit mud. In addition, the bacterial community structure of functional microbial culture liquid by inoculating different quality pit mud was comparative analyzed. This paper illustrated the complexity of the bacteria structure, as well as what causes difference of physiological and biochemical functional of functional microbial in pit mud by researching the perspective of microbial species diversity. The results showed that the quality of pit mud was related to the quality of their functional microbial culture liquid probably. It provided theoretical basis for further improvement of culturing functional microbial in pit mud.
Polymerase chain reaction denaturing gradient gel electrophoresis(PCR-DGGE) was used to analyse the bacterial community structure of different microbial culture liquid,such as the mixed culture liquid of caproic acid bacterial and liquid pit mud, the compound culture liquid of functional microbial in pit mud and the culture liquid of special aroma producing bacteria in pit mud. In addition, the bacterial community structure of functional microbial culture liquid by inoculating different quality pit mud was comparative analyzed. This paper illustrated the complexity of the bacteria structure, as well as what causes difference of physiological and biochemical functional of functional microbial in pit mud by researching the perspective of microbial species diversity. The results showed that the quality of pit mud was related to the quality of their functional microbial culture liquid probably. It provided theoretical basis for further improvement of culturing functional microbial in pit mud.
引文
[1]杜礼泉,王远成,饶家权等.窖泥复合功能菌液培养及应用的研究[J].酿酒,2009(5):35-36.
[2]杜礼泉,饶家权,唐聪等.窖泥功能菌在浓香型大曲酒生产中的应用[J].酿酒,2010(6):43-44.
[3]万联.窖泥功能菌筛选及DYH-1养窖液的应用研究[J].湖北工业大学学报,2011:1-3.
[4]杜礼泉,古加强,唐聪等.己酸菌、液体窖泥的发酵罐混合培养及应用的进一步研究[J].酿酒,2004(1):31-32.
[5]徐军.人工老窖泥培养中功能菌发酵液的制备[J].酿酒科技,2011(10):70-71.
[6]熊翔,杨志龙,方跃进等.超浓缩复合己酸菌液在湘窖酒业窖泥培养中的应用[J].酿酒科技,2011(9):62-64.
[7]赵兴青,杨柳燕,陈灿等.PCR-DGGE技术用于湖泊沉积物中微生物群落结构多样性研究[J].生态学报,2006,11:3610-3616.
[8]林海龙,李伟光,闫险峰等.中药废水污泥群落结构解析中PCR-DGGE引物的选择与评价[J].环境科学,2011,5:1505-1510
[9]Olga Sanchez,Josep M.Gasol,Ramon Massana,et a1.Comparison of different denaturing gradient gel electrophoresis primersets for the study of marine bacterioplankton communities[J].Applied And Environmental Microbiology,2007,73(18):5962-5967.
[2]杜礼泉,饶家权,唐聪等.窖泥功能菌在浓香型大曲酒生产中的应用[J].酿酒,2010(6):43-44.
[3]万联.窖泥功能菌筛选及DYH-1养窖液的应用研究[J].湖北工业大学学报,2011:1-3.
[4]杜礼泉,古加强,唐聪等.己酸菌、液体窖泥的发酵罐混合培养及应用的进一步研究[J].酿酒,2004(1):31-32.
[5]徐军.人工老窖泥培养中功能菌发酵液的制备[J].酿酒科技,2011(10):70-71.
[6]熊翔,杨志龙,方跃进等.超浓缩复合己酸菌液在湘窖酒业窖泥培养中的应用[J].酿酒科技,2011(9):62-64.
[7]赵兴青,杨柳燕,陈灿等.PCR-DGGE技术用于湖泊沉积物中微生物群落结构多样性研究[J].生态学报,2006,11:3610-3616.
[8]林海龙,李伟光,闫险峰等.中药废水污泥群落结构解析中PCR-DGGE引物的选择与评价[J].环境科学,2011,5:1505-1510
[9]Olga Sanchez,Josep M.Gasol,Ramon Massana,et a1.Comparison of different denaturing gradient gel electrophoresis primersets for the study of marine bacterioplankton communities[J].Applied And Environmental Microbiology,2007,73(18):5962-5967.