隐丹参酮对HTR-8/SVneo细胞增殖、凋亡、侵袭、迁移及hCG表达的影响
|
摘要
目的:观察隐丹参酮对HTR-8/SVneo细胞增殖、凋亡、侵袭、迁移及人绒毛膜促性腺激素(hCG)表达的影响。方法:不同浓度的隐丹参酮处理细胞后,CCK8检测细胞增殖,划痕实验检测细胞迁移,transwell实验检测细胞侵袭,流式细胞仪检测细胞凋亡,ELISA检测细胞培养上清hCG表达。结果:与空白组比较,隐丹参酮5、10、20、40μmol/L作用48h可显著抑制HTR-8/SVneo细胞增殖(P<0.01),隐丹参酮20μmol/L可抑制HTR-8/SVneo细胞迁移,隐丹参酮20、40μmol/L作用24h可显著抑制HTR-8/SVneo细胞侵袭(P<0.01),隐丹参酮40μmol/L作用24h可显著诱导HTR-8/SVneo细胞凋亡(P<0.01),隐丹参酮20、40μmol/L作用24h可显著抑制HTR-8/SVneo上清β-hCG表达(P<0.05)。结论:隐丹参酮能抑制HTR-8/SVneo细胞增殖及侵袭迁移,诱导凋亡,并抑制HTR-8/SVneo细胞合成分泌hCG。
Objective: To observe the intervention effects of cryptotanshinone on the proliferation, apoptosis, invasion,migration and human chorionic gonadotropin(hCG) expression of human chorionic trophoblast HTR-8/SVneo cells. Methods:HTR-8/SVneo cells were treated with different concentration of cryptotanshinone, cell proliferation, migration invasion and apoptosis were detected by CCK8, wound scratch assay, transwell and flow cytometry. Enzyme-Linked Immuno Sorbent Assay(ELISA) was used to detect hCG expression in cell supernatant. Results: Compared with the control group, 5, 10, 20 and40μmol/L of 48 h exposure of cryptotanshinone could significantly inhibit the proliferation of HTR-8/SVneo cells(P<0.01),20μmol/L of cryptotanshinone could inhibit the migration of HTR-8/SVneo cells, 20 and 40μmol/L of 24 h exposure of cryptotanshinone could significantly inhibit the invasion of HTR-8/SVneo cells(P<0.01). The apoptosis of HTR-8/SVneo cells was significantly induced by 40μmol/L of 24 h exposure of cryptotanshinone(P<0.01), and the levels of β-hCG in HTR-8/SVneo supernatants was significantly inhibited by 20 and 40μmol/L of 24 h exposure of cryptotanshinone(P<0.05). Conclusion:Cryptotanshinone can inhibit the proliferation, invasion, migration and hCG synthesis and secretion as well as induce apoptosis of HTR-8/SVneo cells.
Objective: To observe the intervention effects of cryptotanshinone on the proliferation, apoptosis, invasion,migration and human chorionic gonadotropin(hCG) expression of human chorionic trophoblast HTR-8/SVneo cells. Methods:HTR-8/SVneo cells were treated with different concentration of cryptotanshinone, cell proliferation, migration invasion and apoptosis were detected by CCK8, wound scratch assay, transwell and flow cytometry. Enzyme-Linked Immuno Sorbent Assay(ELISA) was used to detect hCG expression in cell supernatant. Results: Compared with the control group, 5, 10, 20 and40μmol/L of 48 h exposure of cryptotanshinone could significantly inhibit the proliferation of HTR-8/SVneo cells(P<0.01),20μmol/L of cryptotanshinone could inhibit the migration of HTR-8/SVneo cells, 20 and 40μmol/L of 24 h exposure of cryptotanshinone could significantly inhibit the invasion of HTR-8/SVneo cells(P<0.01). The apoptosis of HTR-8/SVneo cells was significantly induced by 40μmol/L of 24 h exposure of cryptotanshinone(P<0.01), and the levels of β-hCG in HTR-8/SVneo supernatants was significantly inhibited by 20 and 40μmol/L of 24 h exposure of cryptotanshinone(P<0.05). Conclusion:Cryptotanshinone can inhibit the proliferation, invasion, migration and hCG synthesis and secretion as well as induce apoptosis of HTR-8/SVneo cells.
引文
[1]曾金,张志荣,缪萍,等.隐丹参酮的药理作用研究进展.中成药,2015,37(6):1309-1313
[2]Xu J,Wei K Zhang G,et al.Ethnopharmacology,phytochemi stry,and pharmacology of Chinese Salvia species:A review.J Ethnopharmacol,2018,225:18-30
[3]J E D,Pollheimer J,Yong H E,et al.Epithelial-mesenchymal transition during extravillous trophoblast differentiation.Cell Adh Migr,2016,10(3):310-321
[4]DaSilva-Arnold S,James J L,Al-Khan A,et al.Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition.Placenta,2015,36(12):1412-1418
[5]ACOG Practice Bulletin No.191:Tubal Ectopic Pregnancy.Obstet Gynecol,2018,131(2):e65-e77
[6]Ectopic pregnancy-United States,1990-1992.MMWR Morb Mortal Wkly Rep,1995,44(3):46-48
[7]刘磊,张光霁,楼招欢,等.丹参有效成分防治恶性肿瘤作用机制的研究进展.中华中医药杂志,2018,33(6):2472-2475
[8]王加茹,徐宛婷,朴仙姬,等.隐丹参酮诱导人食管癌HCE-4细胞凋亡的机制.肿瘤防治研究,2018,45(8):533-539
[9]叶因涛,钱钧强,杨惠莉,等.隐丹参酮对人乳腺癌MCF-7细胞周期调控因子表达的影响.中国临床药理学杂志,2018,34(8):968-971
[10]邓凤春,于占江,杨钰,等.隐丹参酮对人胃癌细胞SGC-7901凋亡的影响及其机制.北京中医药大学学报,2015,38(7):457-461
[11]Xia C,Bai X,Hou X,et al.Cryptotanshinone reverses cisplatin resistance of human lung carcinoma A549 cells through downregulating Nrf2 pathway.Cell Physiol Biochem,2015,37(2):816-824
[12]朱智杰,刘兆国,周梁,等.隐丹参酮对人乳腺癌细胞MDAMB-231转移的影响及其分子机制.中国实验方剂学杂志,2014,20(11):160-165
[13]全军利,贺文兴,吴斯敏,等.隐丹参酮对乳腺癌MDAMB-231细胞自噬和侵袭迁移的影响.广西医科大学学报,2015,32(1):23-26
[14]Randall S,Buckley C H,Fox H.Placentation in the fallopian tube.Int J Gynecol Pathol,1987,6(2):132-139
[15]Li P,Peng H,Lu W H,et al.Role of Slit2/Robo1 in trophoblast invasion and vascular remodeling during ectopic tubal pregnancy.Placenta,2015,36(10):1087-1094
[16]李晓荣.化瘀消癥杀胚中药诱导输卵管妊娠滋养细胞凋亡的分子机制及临床研究.广州:广州中医药大学,2014
[17]王瑞雪.化瘀消癥杀胚中药复方对输卵管妊娠滋养细胞影响的实验研究.广州:广州中医药大学,2013
[18]谢幸,苟文丽.妇产科学.8版.北京:人民卫生出版社,2013:34
[2]Xu J,Wei K Zhang G,et al.Ethnopharmacology,phytochemi stry,and pharmacology of Chinese Salvia species:A review.J Ethnopharmacol,2018,225:18-30
[3]J E D,Pollheimer J,Yong H E,et al.Epithelial-mesenchymal transition during extravillous trophoblast differentiation.Cell Adh Migr,2016,10(3):310-321
[4]DaSilva-Arnold S,James J L,Al-Khan A,et al.Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition.Placenta,2015,36(12):1412-1418
[5]ACOG Practice Bulletin No.191:Tubal Ectopic Pregnancy.Obstet Gynecol,2018,131(2):e65-e77
[6]Ectopic pregnancy-United States,1990-1992.MMWR Morb Mortal Wkly Rep,1995,44(3):46-48
[7]刘磊,张光霁,楼招欢,等.丹参有效成分防治恶性肿瘤作用机制的研究进展.中华中医药杂志,2018,33(6):2472-2475
[8]王加茹,徐宛婷,朴仙姬,等.隐丹参酮诱导人食管癌HCE-4细胞凋亡的机制.肿瘤防治研究,2018,45(8):533-539
[9]叶因涛,钱钧强,杨惠莉,等.隐丹参酮对人乳腺癌MCF-7细胞周期调控因子表达的影响.中国临床药理学杂志,2018,34(8):968-971
[10]邓凤春,于占江,杨钰,等.隐丹参酮对人胃癌细胞SGC-7901凋亡的影响及其机制.北京中医药大学学报,2015,38(7):457-461
[11]Xia C,Bai X,Hou X,et al.Cryptotanshinone reverses cisplatin resistance of human lung carcinoma A549 cells through downregulating Nrf2 pathway.Cell Physiol Biochem,2015,37(2):816-824
[12]朱智杰,刘兆国,周梁,等.隐丹参酮对人乳腺癌细胞MDAMB-231转移的影响及其分子机制.中国实验方剂学杂志,2014,20(11):160-165
[13]全军利,贺文兴,吴斯敏,等.隐丹参酮对乳腺癌MDAMB-231细胞自噬和侵袭迁移的影响.广西医科大学学报,2015,32(1):23-26
[14]Randall S,Buckley C H,Fox H.Placentation in the fallopian tube.Int J Gynecol Pathol,1987,6(2):132-139
[15]Li P,Peng H,Lu W H,et al.Role of Slit2/Robo1 in trophoblast invasion and vascular remodeling during ectopic tubal pregnancy.Placenta,2015,36(10):1087-1094
[16]李晓荣.化瘀消癥杀胚中药诱导输卵管妊娠滋养细胞凋亡的分子机制及临床研究.广州:广州中医药大学,2014
[17]王瑞雪.化瘀消癥杀胚中药复方对输卵管妊娠滋养细胞影响的实验研究.广州:广州中医药大学,2013
[18]谢幸,苟文丽.妇产科学.8版.北京:人民卫生出版社,2013:34