细胞生物起搏器的研究新进展
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摘要
<正>目前,植入电子起搏器是治疗缓慢心律失常以及病态窦房结综合征的主要方法,虽然在技术上相当娴熟,但它依然存在诸多并发症,如感染、金属过敏、电极脱位、电子干扰和对神经激素缺乏反应性等。这些缺点使得人们迫切需要寻找更接近心脏生理功能的生物起搏器,来进一步提高患者的生活质量[1]。心脏生物起搏是利用细胞分子生物学及其相关技术,对受损的自律性节律点或发生传导障碍的特殊传导系统的组织进行修复或替代,使心脏的起搏和传导功能得以恢
引文
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[13] Kim N,Cho SG. Overcoming immunoregulatory plasticity of mesenchymal stem cells for accelerated clinical applications[J]. Int J Hematol,2016,103(2):129-37.
[14]Xu R,Chen W,Zhang Z,et al. Integrated data analysis identifies potential inducers and pathways during the endothelial differentiation of bone-marrow stromal cells by DNA methyltransferase inhibitor,5-aza-2'-deoxycytidine[J]. Gene,2018,657:9-18.
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[18]Zhang H,Li S,Qu D,et al. Autologous biological pacing function with adrenergic-responsiveness in porcine of complete heart block[J]. Int J Cardiol,2013,168(4):3747-51.
[19] Zhang Xi,Zhang Chuan-Sen,LIU Yan-Chun,et al. Isolation,Culture and Characterization of Cardiac Progenitor Cells Derived from Human Embryonic Heart Tubes[J]. Cells Tissues Organs.2009,190(4):194-208.
[20]李艳君,杨媚,张格格.基因治疗在生物起搏中的研究进展[J].医学研究杂志,2017,46(4):175-8.
[21]Zhou YF,Yang XJ,Li HX,et al. Geneticallyengineered mesenchymal stem cells transfected with human HCN1 gene to create cardiac pacemaker cells[J]. J Int Med Res,2013,41(5):1570-6.
[22]Li Y, Yang M, Zhang G,et al. Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells[J]. Int J Mol Med,2018,41(2):845-51.
[23]张格格,黄从新.转录因子Tbx18、Tbx3、Shox2在生物起搏方面研究进展[J].疑难病杂志,2016,15(4):428-31.
[24] Ionta V,Liang W,Kim EH,et al. Shox2 overexpression favors dIfferentiation of embryonic stem cells into cardiac pacemaker cellsimproving biological pacing ability[J]. Stem Cell Reports,2015,4(1):129-42.
[25]Saito Y,Nakamura K,Yoshida M,et al. Enhancement of spontaneous activity by HCN4 overexpression in mouse embryonic stem cellderived cardiomyocytes-a possible[J]. PLoS One, 2015,10(9):e0138193.
[26]李乐,张城硕,张佳林.细胞融合技术及其应用研究进展[J].辽宁医学杂志,2013(4):195-8.
[27] Cho HC,Kashiwakura Y,Marbán E. Creation of a biological pacemaker by cell fusion[J]. Circ Res,2007,100(8):1112-5.
[28]Gennari M,Gambini E,Bassetti B,et al. Emerging treatment options for refractory angina pectoris:ranolazine, shock wave treatment, and cell-based therapies[J]. Rev Cardiovasc Med, 2014,15(1):31-7.
[2]Nishii N:Arrhythmia management after device removal[J]. J Arrhythm,2016,32(4):287-92.
[3]刘元生.窦房结细胞的钙钟和膜钟[J].临床心电学杂志,2014,23(2):156.
[4]Noam A,Irisawa H. Membrabe currents in the rabbit sinoatrial node cell as studied by the double microelectrode method[J]. Pflugers Arch,1976,364(1):45-52.
[5]成秋平. HCN通道特性研究[D].武汉.华中科技大学,2013.
[6]Boink GJ,Duan L,Nearing BD,et al. HCN2/SkM1 gene transfer into canine left bundle branch induces stable, autonomically responsive biological pacing at physiological heart rates[J]. J Am Coll Cardiol,2013,61(11):1192-201.
[7]Oshita K,Itoh M,Hirashima S,et al. Ectopic automaticity induced in ventricular myocytes by transgenic overexpression of HCN2[J]. J Mol Cell Cardiol,2015,80:81-9.
[8]刘如秀,刘宇,汪艳丽.乳兔窦房结细胞的分离及鉴定[J].中国循证心血管医学杂志,2013,5(2):194-5.
[9]支运霞.去分化脂肪细胞向起搏细胞的诱导分化[D].第二军医大学,2012.
[10] Jun C,Zhihui Z,Lu W,et al. Canine bone marrow mesenchymal stromal cells with lentiviral mHCN4 gene transfer create cardiac pacemakers[J]. Cytotherapy,2012,14(5):529-39.
[11]Zhang L,Li X,Yu X,et al. Construction of vascularized pacemaker tissues by seeding cardiac progenitor cells and endothelial progenitor cells into Matrigel[J]. Life Sci,2017,179:139-46.
[12]吕井井,施国丞,陈会文.心脏干细胞治疗心血管疾病的研究进展[J].中国胸心血管外科临床杂志,2017(12):983-7.
[13] Kim N,Cho SG. Overcoming immunoregulatory plasticity of mesenchymal stem cells for accelerated clinical applications[J]. Int J Hematol,2016,103(2):129-37.
[14]Xu R,Chen W,Zhang Z,et al. Integrated data analysis identifies potential inducers and pathways during the endothelial differentiation of bone-marrow stromal cells by DNA methyltransferase inhibitor,5-aza-2'-deoxycytidine[J]. Gene,2018,657:9-18.
[15]Guhathakurta S,Subramanyan UR,Balasundari R,et al. Stem cell experiments and initial clinical trial of cellular cardiomyoplasty[J].Asian Cardiovasc Thorac Ann,2009,17(6):581-6.
[16]Wang DG,Zhang FX,Chen ML,et al. Cx43 in mesenchymal stem cells promotes angiogenesis of the infarcted heart independent of gap junctions[J]. Mol Med Rep,2014,9(4):1095-102.
[17]蒋伟.骨髓间充质干细胞诱导分化为具有起搏功能的心肌样细胞[D].郑州大学,2010.
[18]Zhang H,Li S,Qu D,et al. Autologous biological pacing function with adrenergic-responsiveness in porcine of complete heart block[J]. Int J Cardiol,2013,168(4):3747-51.
[19] Zhang Xi,Zhang Chuan-Sen,LIU Yan-Chun,et al. Isolation,Culture and Characterization of Cardiac Progenitor Cells Derived from Human Embryonic Heart Tubes[J]. Cells Tissues Organs.2009,190(4):194-208.
[20]李艳君,杨媚,张格格.基因治疗在生物起搏中的研究进展[J].医学研究杂志,2017,46(4):175-8.
[21]Zhou YF,Yang XJ,Li HX,et al. Geneticallyengineered mesenchymal stem cells transfected with human HCN1 gene to create cardiac pacemaker cells[J]. J Int Med Res,2013,41(5):1570-6.
[22]Li Y, Yang M, Zhang G,et al. Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells[J]. Int J Mol Med,2018,41(2):845-51.
[23]张格格,黄从新.转录因子Tbx18、Tbx3、Shox2在生物起搏方面研究进展[J].疑难病杂志,2016,15(4):428-31.
[24] Ionta V,Liang W,Kim EH,et al. Shox2 overexpression favors dIfferentiation of embryonic stem cells into cardiac pacemaker cellsimproving biological pacing ability[J]. Stem Cell Reports,2015,4(1):129-42.
[25]Saito Y,Nakamura K,Yoshida M,et al. Enhancement of spontaneous activity by HCN4 overexpression in mouse embryonic stem cellderived cardiomyocytes-a possible[J]. PLoS One, 2015,10(9):e0138193.
[26]李乐,张城硕,张佳林.细胞融合技术及其应用研究进展[J].辽宁医学杂志,2013(4):195-8.
[27] Cho HC,Kashiwakura Y,Marbán E. Creation of a biological pacemaker by cell fusion[J]. Circ Res,2007,100(8):1112-5.
[28]Gennari M,Gambini E,Bassetti B,et al. Emerging treatment options for refractory angina pectoris:ranolazine, shock wave treatment, and cell-based therapies[J]. Rev Cardiovasc Med, 2014,15(1):31-7.