腐胺对沼泽小叶桦硬枝扦插生根和生长的影响
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摘要
以沼泽小叶桦1年生硬枝插条为材料,研究了3种浓度腐胺(50、100、200 mg·L~(-1))和3种处理时间(5、30、120 min)下的扦插生根率、扦插苗的生长和生物量情况,旨在为沼泽小叶桦扦插育苗提供参考。结果表明:不同处理间的扦插生根率差异显著(P<0.05),腐胺50 mg·L~(-1)+30 min处理和腐胺100 mg·L~(-1)+5 min处理的扦插生根率最高,达92.2%;适宜的腐胺浓度和处理时间能提高沼泽小叶桦的扦插生根率,但对苗木地径、苗高、根长和各器官生物量的作用很小,影响苗木生长量的主要因子是光照条件和养分状况。
To improve the cutting method of Betula microphylla var. paludosa,hardwood cuttings( one year old) of B. microphylla var. paludosa were used as test materials. The rooting rate,seedling growth,and biomass were analyzed under various treatments including three putrescine concentrations( 50,100,and 200 mg·L~(-1)) and three soaking times( 5,30,and 120 min). The obtained results demonstrated that the rooting rate of the cuttings were significantly different for the various treatments( P< 0.05).The results obtained for the putrescine treatments of 50 mg·L~(-1)+30 min and 100 mg·L~(-1)+5 min were found to be the best,with both achieving a 92. 2% rooting rate for the cuttings. An appropriate concentration and soaking time of putrescine could improve the rooting rates of B. microphylla var. paludosa cuttings. However,the putrescine treatments could not affect the ground diameter,height,root length,and biomass of the seedlings. The light and nutrient conditions were the main factors that affected the growth of the seedlings.
To improve the cutting method of Betula microphylla var. paludosa,hardwood cuttings( one year old) of B. microphylla var. paludosa were used as test materials. The rooting rate,seedling growth,and biomass were analyzed under various treatments including three putrescine concentrations( 50,100,and 200 mg·L~(-1)) and three soaking times( 5,30,and 120 min). The obtained results demonstrated that the rooting rate of the cuttings were significantly different for the various treatments( P< 0.05).The results obtained for the putrescine treatments of 50 mg·L~(-1)+30 min and 100 mg·L~(-1)+5 min were found to be the best,with both achieving a 92. 2% rooting rate for the cuttings. An appropriate concentration and soaking time of putrescine could improve the rooting rates of B. microphylla var. paludosa cuttings. However,the putrescine treatments could not affect the ground diameter,height,root length,and biomass of the seedlings. The light and nutrient conditions were the main factors that affected the growth of the seedlings.
引文
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[18]洪汉辉,康向阳,汪晓峰.年龄效应对白杨硬枝扦插苗生长及其茎皮部解剖结构和叶片生化指标的影响[J].西北植物学报,2018,38(2):274-281.
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[20]金国庆,秦国峰,储德裕,等.杂种马褂木扦插繁殖技术的研究[J].林业科学研究,2006,19(3):370-375.
[21]敖红,王昆,冯玉龙.长白落叶松插穗的内源激素水平及其与扦插生根的关系[J].植物研究,2002,22(2):190-195.
[22]孙敬爽,郑红娟,贾桂霞,等.不同基质、生长调节剂、插穗规格和代谢调节剂对‘蓝星’扦插生根的影响[J].北京林业大学学报,2008,30(1):67-73.
[23]骆畅,刘济明,黄小龙,等.不同基质、生长调节剂及部位对米槁扦插生根的影响[J].四川大学学报(自然科学版),2018,55(4):843-847.
[24]薛秀康.不同基质对湿地松容器苗生长的影响[J].林业科技通讯,1993(3):17-20.
[25]向青云,黎明,张发维,等.柑橘大棚容器育苗基质配比试验[J].种子,2007,26(2):75-77.
[2]有祥亮,崔心红,张群,等.沼泽小叶桦嫩枝扦插试验[J].上海交通大学学报(农业科学版),2012,29(2):12-16.
[3]曹敏.多胺及其植物生理学作用[J].植物生理学通讯,1984,20(6):1-5.
[4]彭向永,程运河,李振坚,等.蒿柳成花过程中内源激素和多胺含量变化特征[J].林业科学,2018,54(8):39-47.
[5]郝楠,王成,李月明.多胺代谢与玉米低温胁迫的研究进展[J].园艺与种苗,2018(8):53-56.
[6]李霞,程运河,马晓东,等.多胺在植物抗逆中的生理机制[J].世界林业研究,2018,31(4):23-28.
[7]BIONDI S,SCARAMAGLI S,CAPITANI F,et al.Methyl jasmonate upregulates biosynthetic gene expression,oxidation and conjugation of polyamines,and inhibits shoot formation in tobacco thin layers[J].Journal of Experimental Botany,2001,52(355):231-242.
[8]HAUSMAN J F,KEVERS C,GASPAR T.Auxin-polyamine interaction in the control of the rooting inductive phase of poplar shoots in vitro[J].Plant Science,1995,110(1):63-71.
[9]刘鑫,李晓彤,荆鑫,等.S-腺苷甲硫氨酸对黄瓜断根扦插苗生长及生理代谢的影响[J].园艺学报,2018,45(8):1 513-1 522.
[10]李际红,孟凡志,刘海燕,等.稀土及多胺对欧石楠瓶苗生根的效应[J].山东农业大学学报(自然科学版),2012,43(2):169-173.
[11]NAG S,SAHA K,CHOUDHURI M A.Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting[J].Journal of Plant Growth Regulation,2001,20(2):182-194.
[12]REY M,DIAZ-SALA C,RODRIGUEZ R.Exogenous polyamines improve rooting of hazel microshoots[J].Plant Cell,Tissue and Organ Culture,1994,36(3):303-308.
[13]DENAXA N K,ROUSSOS P A,VEMMOS S N.The possible role of polyamines to the recalcitrance of“Kalamata”olive leafy cuttings to root[J].Journal of Plant Growth Regulation,2014,33(3):579-589.
[14]杨洪强,黄天栋,束怀瑞,等.外源IBA和PAs对苹果幼苗新根形成、多胺及核酸含量的影响[J].园艺学报,1995,22(2):123-126.
[15]徐东花.亚精胺对菊花不定根发生及生根机理的研究[D].泰安:山东农业大学,2015.
[16]魏茂胜.不同处理对茶绒杜鹃扦插生根与生长的影响[J].森林与环境学报,2019,39(1):27-31.
[17]唐罗忠,赵丹,田野,等.不同处理下的麻栎扦插成活率和内含物含量[J].福建林学院学报,2012,32(2):113-119.
[18]洪汉辉,康向阳,汪晓峰.年龄效应对白杨硬枝扦插苗生长及其茎皮部解剖结构和叶片生化指标的影响[J].西北植物学报,2018,38(2):274-281.
[19]郑健,郑勇奇,吴超.花楸树嫩枝扦插繁殖技术研究[J].林业科学研究,2009,22(1):91-97.
[20]金国庆,秦国峰,储德裕,等.杂种马褂木扦插繁殖技术的研究[J].林业科学研究,2006,19(3):370-375.
[21]敖红,王昆,冯玉龙.长白落叶松插穗的内源激素水平及其与扦插生根的关系[J].植物研究,2002,22(2):190-195.
[22]孙敬爽,郑红娟,贾桂霞,等.不同基质、生长调节剂、插穗规格和代谢调节剂对‘蓝星’扦插生根的影响[J].北京林业大学学报,2008,30(1):67-73.
[23]骆畅,刘济明,黄小龙,等.不同基质、生长调节剂及部位对米槁扦插生根的影响[J].四川大学学报(自然科学版),2018,55(4):843-847.
[24]薛秀康.不同基质对湿地松容器苗生长的影响[J].林业科技通讯,1993(3):17-20.
[25]向青云,黎明,张发维,等.柑橘大棚容器育苗基质配比试验[J].种子,2007,26(2):75-77.