大白菜抗根肿病的抗源筛选和分子标记鉴定
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摘要
以3种根肿菌M01、M02、M03为病原菌菌源,对24份大白菜材料进行接种鉴定和分子标记鉴定,旨在获得优良的抗源材料并明确材料中含有的抗病基因。结果表明:24份大白菜材料中共有10份抗病材料,5份材料对3种根肿菌都表现抗病:其中CR004和CR007含有根肿病抗病基因CRk,CR013含有抗病基因CRa、CRb~(kato)、CRk和Crr3,CR015和CR016含有抗病基因CRa、CRb~(kato)和Crr3;3份材料对根肿菌M01和M02表现抗病:CR012含有抗病基因CRa和CRb~(kato),CR014和CR018含有抗病基因CRa、CRb~(kato)和CRk;抗根肿菌M01的材料CR021含有抗病基因Crr2和CRk;抗根肿菌M02的材料CR023含有抗病基因CRc和CRk。
Twenty-four Chinese cabbage(Brassica rapa L. ssp. chinensis)materials were inoculated with 3 kinds of Plasmodiophora brassicae M01,M02 and M03,and all the lines were identified by molecular markers in order to obtain excellent anti-sources and to confirm all materials containing disease resistant genes. The results showed that there were 10 out of 24 materials were resistant to disease. Among them 5 were resistant to 3 kinds of Plasmodiophora. Lines CR004 and CR007 contained the disease resistant gene CRk,CR013 containing disease resistant genes CRa,CRb~(kato),CRk and Crr3. CR015 and CR016 contained the disease resistant genes CRa,CRb~(kato) and Crr3. All 3 materials showed resistance to M01 and M02. Line CR012 contained disease resistant genes CRa and CRb~(kato). CR014 and CR018 contained disease resistant genes CRa,CRb~(kato) and CRk. The CR021 line contained disease resistant genes Crr2 and CRk. The CR023 line contained disease resistant genes CRc and CRk showed resistance to M02.
Twenty-four Chinese cabbage(Brassica rapa L. ssp. chinensis)materials were inoculated with 3 kinds of Plasmodiophora brassicae M01,M02 and M03,and all the lines were identified by molecular markers in order to obtain excellent anti-sources and to confirm all materials containing disease resistant genes. The results showed that there were 10 out of 24 materials were resistant to disease. Among them 5 were resistant to 3 kinds of Plasmodiophora. Lines CR004 and CR007 contained the disease resistant gene CRk,CR013 containing disease resistant genes CRa,CRb~(kato),CRk and Crr3. CR015 and CR016 contained the disease resistant genes CRa,CRb~(kato) and Crr3. All 3 materials showed resistance to M01 and M02. Line CR012 contained disease resistant genes CRa and CRb~(kato). CR014 and CR018 contained disease resistant genes CRa,CRb~(kato) and CRk. The CR021 line contained disease resistant genes Crr2 and CRk. The CR023 line contained disease resistant genes CRc and CRk showed resistance to M02.
引文
柴阿丽,朱发娣,王惟萍,石延霞,谢学文,李宝聚.2015.十字花科根肿病接种技术及发病条件研究.华北农学报,(S1):266-271.
丁云花,简元才,余阳俊,汪维红,耿丽华,康俊根.2013.我国8省市十字花科蔬菜根肿菌生理小种的鉴定.中国蔬菜,(16):85-88.
李宁,赵利民,鱼昭君,陈红红,惠麦侠.2015.大白菜品种对陕西省太白县根肿病的抗性鉴定.中国农学通报,31(34):173-176.
刘峰,张丽辉,姬广海.2013.云南和西藏十字花科蔬菜根肿菌生理小种鉴定.中国蔬菜,(20):77-78.
彭沙莎,任佐华,黄小莉,刘敏捷,孙良菲,刘二明.2013.湖南十字花科作物根肿菌生理小种鉴定.长江蔬菜,(6):46-49.
朴钟云,吴迪,王淼,张腾.2010.大白菜抗根肿病近等基因系的分子标记辅助选育.园艺学报,37(8):1264-1272.
沈向群,聂凯,吴琼,张玉光,孟星河.2009.大白菜根肿病主要生理小种种群分化鉴定初报.中国蔬菜,(8):59-62.
孙保亚,沈向群,郭海峰,周永红.2005.十字花科植物根肿病及抗病育种研究进展.中国蔬菜,(4):34-37.
王彤彤,张淑江,章时蕃,李菲,张慧,孙日飞.2012.大白菜根肿病抗性基因的标记和定位.中国蔬菜,(14):31-35.
杨永林.1990.十字花科蔬菜根肿病抑菌型土壤初探.植物保护学报,17(2):127-131.
Chen J,Jing J,Zhan Z.2013.Identification of novel QTLs for isolatespecific partial resistance to Plasmodiophora brassica in Brassica rapa.PLo S One,8(12):e85307.
Chen L,Zhang X,Xu H.2016.Introgression of clubroot resistance into an elite pak choi inbred line through marker-assisted introgression breeding.Plant Breeding,135(4):471-475.
Chu M,Song T,falk K,Zhang X,Liu X,Chang A.2014.Fine mapping of Rcr1 and analyses of its effect on transcriptome patterns during infection by Plasmodiophora brassicae.BMC Genomics,10.1186/1471-2164-15-1166.
Gao F,Hirani A H,Liu J,Liu Z,Fu G,Wu C,Peter B E,Mc Vetty P B E,Li G.2014.Fine mapping a clubroot resistance locus in Chinese cabbage.J Amer Soc Hort Sci,139(3):247-252.
Hatakeyama K,Niwa T,Kato T,Ohara T,Kakizaki T,Matsumoto S.2017.The tandem repeated organization of NB-LRR genes in the clubroot-resistant CRb locus in Brassica rapa L.Molecular Genetics and Genomics.292(2):397-405.
Hayashida N,Takabatake Y,Nakazawa N.2008.Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage,with intensive analysis of HC352b genes.Journal of the Japanese Society for Horticultural Science,77(2):150-154.
Hirai M,Harada T,Kubo N,sukada M,Suwabe K,Matsumoto S.2004.A novel locus for clubroot resistance in Brassica rapa and its linkage markers.Theoretical and Applied Genetics,108(4):639-643.
Karling J S.1968.The plasmodiophorales:including a complete host index,bibliography,and a description of diseases caused by species of this order.Molecular Biology&Evolution,22(3):582-588.
Kato T,Hatakeyama K,Fukino N,Matsumoto S.2013.Fine mapping of the clubroot resistance gene CRb and development of a useful selectable maker in Brassica rapa.Breeding Sci,6(3):116-124.
Kawamura K,Shimizu M,Kawanabe T.2017.Assessment of DNAmarkers for seed contamination testing and selection of disease resistance in cabbage.Euphytica,213(1):28.
Kuginuki Y,Yoshikawa H,Hirai M.1999.Variation in virulence of Plasmodiophora brassicae in Japan tested with clubrootresistant cultivars of Chinese cabbage(Brassica rapa L.ssp.pekinensis).European Journal of Plant Pathology,105(4):327-332.
Liu B,Wang Y,Zhai W,Deng J,Wang H,Cui Y,Cheng F,Wang X W,Wu J.2013.Development of In Del markers for Brassica rapa based on whole-genome re-sequencing.Theoretical and Applied Genetics,126(1):231-239.
Matsumoto E S,Ueno H S,Aruga D.2012.Accumulation of three clubroot resistance genes through marker-assisted selection in Chinese cabbage(Brassica rapa ssp.pekinensis).Journal of the Japanese Society for Horticultural Sciences,81(2):184-190.
McCouch S R,Chen X,Panaud O.1997.Microsatellite marker development,mapping and applications in rice genetics and breeding.Plant Molecular Biology,35:89-99.
Murray M G,Thompson W F.1980.Rapid isolation of high-molecular weight plant DNA.Nucleic Acids Research,8:4321-4325.
Piao Z Y,Deng Y Q,Choi S R.2004.SCAR and CAPS mapping of CRb,a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage(Brassica rapa ssp.pekinensis).Theoretical and Applied Genetics,108(8):1458-1465.
Saito M,Kubo N,Matsumoto S,Suwabe K,Tsukada M,Hirai M.2006.Fine mapping of the clubroot resistance gene,crr3,in Brassica rapa.Theor Appl Genet,114:81-91.
Sakamoto K,Saito A,Hayashida N,Taguchi G,Matsumoto E.2008.Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage(Brassica rapa L.ssp.pekinensis).Theoretical and Applied Genetics,117(5):759-767.
Suwabe K,Tsukazaki H,Lketani H.2003.Identification of two loci for resistance to clubroot(Plasmodiophoru brassicae Worenin)in Brassica rapa L.Theoretical and Applied Genetics,107(6):997-1002.
Suwabe K,Tsukazaki H,Iketani H,Hatakeyama K,Kondo M,Fujimura M,Nunome T,Fukuoka H,Hirai M,Matsumoto S.2006.Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:the genetic origin of clubroot resistance.Genetics,173(1):309-319.
Ueno H,Matsumoto E,Aruga D,Kitagawa S,Matsumura H,Hayashida N.2012.Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa.Plant Mol Biol,80:621-629.
Zhang T,Zhao Z,Zhang C.2014.Fine genetic and physical mapping of the CRb gene conferring resistance to clubroot disease in Brassica rapa.Molecular Breeding,34(3):1173-1183.
Zhang H,Feng J,Zhang S.2015.Resistance to Plasmodiophora brassicae in Brassica rapa and Brassica juncea genotypes from China.Plant Disease,99(6):776-779.
丁云花,简元才,余阳俊,汪维红,耿丽华,康俊根.2013.我国8省市十字花科蔬菜根肿菌生理小种的鉴定.中国蔬菜,(16):85-88.
李宁,赵利民,鱼昭君,陈红红,惠麦侠.2015.大白菜品种对陕西省太白县根肿病的抗性鉴定.中国农学通报,31(34):173-176.
刘峰,张丽辉,姬广海.2013.云南和西藏十字花科蔬菜根肿菌生理小种鉴定.中国蔬菜,(20):77-78.
彭沙莎,任佐华,黄小莉,刘敏捷,孙良菲,刘二明.2013.湖南十字花科作物根肿菌生理小种鉴定.长江蔬菜,(6):46-49.
朴钟云,吴迪,王淼,张腾.2010.大白菜抗根肿病近等基因系的分子标记辅助选育.园艺学报,37(8):1264-1272.
沈向群,聂凯,吴琼,张玉光,孟星河.2009.大白菜根肿病主要生理小种种群分化鉴定初报.中国蔬菜,(8):59-62.
孙保亚,沈向群,郭海峰,周永红.2005.十字花科植物根肿病及抗病育种研究进展.中国蔬菜,(4):34-37.
王彤彤,张淑江,章时蕃,李菲,张慧,孙日飞.2012.大白菜根肿病抗性基因的标记和定位.中国蔬菜,(14):31-35.
杨永林.1990.十字花科蔬菜根肿病抑菌型土壤初探.植物保护学报,17(2):127-131.
Chen J,Jing J,Zhan Z.2013.Identification of novel QTLs for isolatespecific partial resistance to Plasmodiophora brassica in Brassica rapa.PLo S One,8(12):e85307.
Chen L,Zhang X,Xu H.2016.Introgression of clubroot resistance into an elite pak choi inbred line through marker-assisted introgression breeding.Plant Breeding,135(4):471-475.
Chu M,Song T,falk K,Zhang X,Liu X,Chang A.2014.Fine mapping of Rcr1 and analyses of its effect on transcriptome patterns during infection by Plasmodiophora brassicae.BMC Genomics,10.1186/1471-2164-15-1166.
Gao F,Hirani A H,Liu J,Liu Z,Fu G,Wu C,Peter B E,Mc Vetty P B E,Li G.2014.Fine mapping a clubroot resistance locus in Chinese cabbage.J Amer Soc Hort Sci,139(3):247-252.
Hatakeyama K,Niwa T,Kato T,Ohara T,Kakizaki T,Matsumoto S.2017.The tandem repeated organization of NB-LRR genes in the clubroot-resistant CRb locus in Brassica rapa L.Molecular Genetics and Genomics.292(2):397-405.
Hayashida N,Takabatake Y,Nakazawa N.2008.Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage,with intensive analysis of HC352b genes.Journal of the Japanese Society for Horticultural Science,77(2):150-154.
Hirai M,Harada T,Kubo N,sukada M,Suwabe K,Matsumoto S.2004.A novel locus for clubroot resistance in Brassica rapa and its linkage markers.Theoretical and Applied Genetics,108(4):639-643.
Karling J S.1968.The plasmodiophorales:including a complete host index,bibliography,and a description of diseases caused by species of this order.Molecular Biology&Evolution,22(3):582-588.
Kato T,Hatakeyama K,Fukino N,Matsumoto S.2013.Fine mapping of the clubroot resistance gene CRb and development of a useful selectable maker in Brassica rapa.Breeding Sci,6(3):116-124.
Kawamura K,Shimizu M,Kawanabe T.2017.Assessment of DNAmarkers for seed contamination testing and selection of disease resistance in cabbage.Euphytica,213(1):28.
Kuginuki Y,Yoshikawa H,Hirai M.1999.Variation in virulence of Plasmodiophora brassicae in Japan tested with clubrootresistant cultivars of Chinese cabbage(Brassica rapa L.ssp.pekinensis).European Journal of Plant Pathology,105(4):327-332.
Liu B,Wang Y,Zhai W,Deng J,Wang H,Cui Y,Cheng F,Wang X W,Wu J.2013.Development of In Del markers for Brassica rapa based on whole-genome re-sequencing.Theoretical and Applied Genetics,126(1):231-239.
Matsumoto E S,Ueno H S,Aruga D.2012.Accumulation of three clubroot resistance genes through marker-assisted selection in Chinese cabbage(Brassica rapa ssp.pekinensis).Journal of the Japanese Society for Horticultural Sciences,81(2):184-190.
McCouch S R,Chen X,Panaud O.1997.Microsatellite marker development,mapping and applications in rice genetics and breeding.Plant Molecular Biology,35:89-99.
Murray M G,Thompson W F.1980.Rapid isolation of high-molecular weight plant DNA.Nucleic Acids Research,8:4321-4325.
Piao Z Y,Deng Y Q,Choi S R.2004.SCAR and CAPS mapping of CRb,a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage(Brassica rapa ssp.pekinensis).Theoretical and Applied Genetics,108(8):1458-1465.
Saito M,Kubo N,Matsumoto S,Suwabe K,Tsukada M,Hirai M.2006.Fine mapping of the clubroot resistance gene,crr3,in Brassica rapa.Theor Appl Genet,114:81-91.
Sakamoto K,Saito A,Hayashida N,Taguchi G,Matsumoto E.2008.Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage(Brassica rapa L.ssp.pekinensis).Theoretical and Applied Genetics,117(5):759-767.
Suwabe K,Tsukazaki H,Lketani H.2003.Identification of two loci for resistance to clubroot(Plasmodiophoru brassicae Worenin)in Brassica rapa L.Theoretical and Applied Genetics,107(6):997-1002.
Suwabe K,Tsukazaki H,Iketani H,Hatakeyama K,Kondo M,Fujimura M,Nunome T,Fukuoka H,Hirai M,Matsumoto S.2006.Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:the genetic origin of clubroot resistance.Genetics,173(1):309-319.
Ueno H,Matsumoto E,Aruga D,Kitagawa S,Matsumura H,Hayashida N.2012.Molecular characterization of the CRa gene conferring clubroot resistance in Brassica rapa.Plant Mol Biol,80:621-629.
Zhang T,Zhao Z,Zhang C.2014.Fine genetic and physical mapping of the CRb gene conferring resistance to clubroot disease in Brassica rapa.Molecular Breeding,34(3):1173-1183.
Zhang H,Feng J,Zhang S.2015.Resistance to Plasmodiophora brassicae in Brassica rapa and Brassica juncea genotypes from China.Plant Disease,99(6):776-779.