白菜抗芜菁花叶病毒(TuMV)鉴定及抗源筛选
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摘要
[目的]筛选出抗芜菁花叶病毒(Tu MV)的白菜种质资源。[方法]选取8份白菜高代自交系种质材料,在3叶期人工磨擦接种Tu MV-C4株系,进行抗/感病表型调查、d CAPS功能标记筛选以及ELISA试验鉴定。[结果]1600011-1、1600043-3、1600045-3和1600331-3为Tu MV感病材料;1600031-2、1600067-2、1600105-1和1600485-2为Tu MV抗病材料。[结论 ]研究鉴定了大白菜抗Tu MV的性状,为大白菜抗Tu MV育种提供了抗源材料。
[Objective] In order to screen germplasm resources resistant to Turnip mosaic virus(Tu MV)in Chinese cabbage. [Method] Eight Chinese cabbage highly inbred lines were inoculated with Tu MV-C4 isolate to screen the resistance lines,when the plants were at 3-leave stage.[Result] Thronging phenotype investigating,d CAPS marker screening,and the ELISA assay identifying,it confirmed that the four lines:1600011-1、1600043-3、1600045-3 and 1600331-3 were susceptible materials, and the four lines:1600031-2、1600067-2、1600105-1 and 1600485-2 were resistant materials. [Conclusion] The study screened the germplasm resources resistant to Tu MV in Chinese cabbage using three methods,and provided the resistance materials for resistance Tu MV breeding.
[Objective] In order to screen germplasm resources resistant to Turnip mosaic virus(Tu MV)in Chinese cabbage. [Method] Eight Chinese cabbage highly inbred lines were inoculated with Tu MV-C4 isolate to screen the resistance lines,when the plants were at 3-leave stage.[Result] Thronging phenotype investigating,d CAPS marker screening,and the ELISA assay identifying,it confirmed that the four lines:1600011-1、1600043-3、1600045-3 and 1600331-3 were susceptible materials, and the four lines:1600031-2、1600067-2、1600105-1 and 1600485-2 were resistant materials. [Conclusion] The study screened the germplasm resources resistant to Tu MV in Chinese cabbage using three methods,and provided the resistance materials for resistance Tu MV breeding.
引文
[1]WALSH J A,SHARPE A G,JENNER C E,et al.Characterisation of resistance to turnip mosaic virus in oilseed rape(Brassica napus)and genetic mapping of Turb01[J].Theoretical&Applied Genetics,1999,99(7-8):1149-1154.
[2]WALSH J A,JENNER C E.Turnip mosaic virus and the quest for durable resistance[J].Molecular Plant Pathology,2002,3(5):289.
[3]李巧云,张志刚,刘栓桃,等.大白菜芜菁花叶病毒病抗性遗传分析[J].华北农学报,2012,27(4):135-139.
[4]PROVVIDENTI R.Evaluation of Chinese cabbage cultivars from japan and the people's republic of china for resistance to turnip mosaic virus and cauliflower mosaic virus[J].Journal of the American Society for Horticultural Science,1980,105(4):571-573.
[5]GREEN S K,DENG T C.Turnip mosaic virus strains in cruciferous hosts in taiwan[J].Plant Disease,1985,69:28-31.
[6]冯兰香,徐玲,刘佳,等.北京地区大白菜芜菁花叶病毒株系的鉴定[J].中国蔬菜,1988,1(4):4-10.
[7]陈集双,吴林福,周雪平,等.美人蕉黄瓜花叶病毒研究[J].浙江农业学报,1994(4):272-275.
[8]蔡丽,许泽永,陈坤荣,等.芜菁花叶病毒研究进展[J].湖北植保,2004,27(5):104-110.
[9]李国亮.白菜e IF(iso)4E基因对Tu MV的抗性机制研究[D].北京:中国农业科学院,2016.
[10]李烨,罗玉明.不同萝卜品种对Tu MV抗性的苗期鉴定[J].长江蔬菜,2000(12),24-26.
[11]钱伟.大白菜Tu MV抗性基因retr02的克隆与抗性机制研究[D].北京:中国农业科学院,2013.
[12]LI G,QIAN W,ZHANG S,et al.Development of gene-based markers for the turnip mosaic virus resistance gene retr02 in Brassica rapa[J].Plant Breeding,2016,135(4):466-470.
[13]施曼玲,周雪平.植物病毒病的诊断技术[J].微生物学通报,2000,27(2):149-151.
[14]李巧云,张志刚,成文华,等.利用ELISA方法鉴定大白菜Tu MV抗性[J].科技导报,2009,27(1):42-45.
[15]韩和平.大白菜抗芜菁花叶病毒病基因的AFLP分子标记的研究[D].北京:中国农业科学院,2003.
[16]JENNER C E,NELLIST C F,BARKER G C,et al.Turnip mosaic virus(Tu MV)is able to use alleles of both e IF4E and e IF(iso)4E from multiple loci of the diploid Brassica rapa[J].Molecular plant-microbe interactions,MPMI,2010,23(11):1498.
[17]NELLIST C F,QIAN W,JENNER C E,et al.Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy,enabling diversification through variation in splicing and broad-spectrum virus resistance[J].Plant Journal for Cell&Molecular Biology,2014,77(2):261-268.
[18]JENNER C E,S魣NCHEZ F,NETTLESHIP S B,et al.The cylindrical inclusion gene of turnip mosaic virus encodes a pathogenic determinant to the brassica resistance gene turb01[J].Molecular plantmicrobe interactions,2000,13(10):1102.
[19]WALSH J A,RUSHOLME R L,HUGHES S L,et al.Different classes of resistance to turnip mosaic virus in Brassica rapa[J].European Journal of Plant Pathology,2002,108(1):15-20.
[20]HUGHES S L,HUNTER P J,SHARPE A G,et al.Genetic mapping of the novel turnip mosaic virus resistance gene turb03 in brassica napus[J].Theoretical&Applied Genetics,2003,107(7):1169.
[21]JENNER C E,WANG X,PONZ F,et al.A fitness cost for turnip mosaic virus to overcome host resistance[J].Virus Research,2002,86(1-2):1-6.
[2]WALSH J A,JENNER C E.Turnip mosaic virus and the quest for durable resistance[J].Molecular Plant Pathology,2002,3(5):289.
[3]李巧云,张志刚,刘栓桃,等.大白菜芜菁花叶病毒病抗性遗传分析[J].华北农学报,2012,27(4):135-139.
[4]PROVVIDENTI R.Evaluation of Chinese cabbage cultivars from japan and the people's republic of china for resistance to turnip mosaic virus and cauliflower mosaic virus[J].Journal of the American Society for Horticultural Science,1980,105(4):571-573.
[5]GREEN S K,DENG T C.Turnip mosaic virus strains in cruciferous hosts in taiwan[J].Plant Disease,1985,69:28-31.
[6]冯兰香,徐玲,刘佳,等.北京地区大白菜芜菁花叶病毒株系的鉴定[J].中国蔬菜,1988,1(4):4-10.
[7]陈集双,吴林福,周雪平,等.美人蕉黄瓜花叶病毒研究[J].浙江农业学报,1994(4):272-275.
[8]蔡丽,许泽永,陈坤荣,等.芜菁花叶病毒研究进展[J].湖北植保,2004,27(5):104-110.
[9]李国亮.白菜e IF(iso)4E基因对Tu MV的抗性机制研究[D].北京:中国农业科学院,2016.
[10]李烨,罗玉明.不同萝卜品种对Tu MV抗性的苗期鉴定[J].长江蔬菜,2000(12),24-26.
[11]钱伟.大白菜Tu MV抗性基因retr02的克隆与抗性机制研究[D].北京:中国农业科学院,2013.
[12]LI G,QIAN W,ZHANG S,et al.Development of gene-based markers for the turnip mosaic virus resistance gene retr02 in Brassica rapa[J].Plant Breeding,2016,135(4):466-470.
[13]施曼玲,周雪平.植物病毒病的诊断技术[J].微生物学通报,2000,27(2):149-151.
[14]李巧云,张志刚,成文华,等.利用ELISA方法鉴定大白菜Tu MV抗性[J].科技导报,2009,27(1):42-45.
[15]韩和平.大白菜抗芜菁花叶病毒病基因的AFLP分子标记的研究[D].北京:中国农业科学院,2003.
[16]JENNER C E,NELLIST C F,BARKER G C,et al.Turnip mosaic virus(Tu MV)is able to use alleles of both e IF4E and e IF(iso)4E from multiple loci of the diploid Brassica rapa[J].Molecular plant-microbe interactions,MPMI,2010,23(11):1498.
[17]NELLIST C F,QIAN W,JENNER C E,et al.Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy,enabling diversification through variation in splicing and broad-spectrum virus resistance[J].Plant Journal for Cell&Molecular Biology,2014,77(2):261-268.
[18]JENNER C E,S魣NCHEZ F,NETTLESHIP S B,et al.The cylindrical inclusion gene of turnip mosaic virus encodes a pathogenic determinant to the brassica resistance gene turb01[J].Molecular plantmicrobe interactions,2000,13(10):1102.
[19]WALSH J A,RUSHOLME R L,HUGHES S L,et al.Different classes of resistance to turnip mosaic virus in Brassica rapa[J].European Journal of Plant Pathology,2002,108(1):15-20.
[20]HUGHES S L,HUNTER P J,SHARPE A G,et al.Genetic mapping of the novel turnip mosaic virus resistance gene turb03 in brassica napus[J].Theoretical&Applied Genetics,2003,107(7):1169.
[21]JENNER C E,WANG X,PONZ F,et al.A fitness cost for turnip mosaic virus to overcome host resistance[J].Virus Research,2002,86(1-2):1-6.