传统中药百药煎中没食子酸测定方法的研究
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摘要
没食子酸是传统中药百药煎的主要成分之一,本文开展了HPLC法测定百药煎中没食子酸含量的方法学研究。通过试验考察了提取溶剂、提取体积、提取时间、提取次数对没食子酸含量测定结果的影响,确定了最优的提取条件(20 mL 50%甲醇为提取溶剂,超声提取20 min,提取一次)。在此提取条件下,开展了HPLC测定法的线性范围、仪器精密度试验、溶液稳定性试验、重复性试验、准确度(回收率)试验和耐用性试验等研究,结果表明本文开发的HPLC是传统中药百药煎中没食子酸含量测定的良好方法。
Gallic acid is one of main ingredients of traditional Chinese gall leaven. This study was conducted to develop a method for determination of gallic acid content in traditional Chinese gall leaven by HPLC. The effects of different extraction solvent, extraction volume, extraction time and extraction timeson gallic acid extraction were investigated and the optimal extraction condition was confirmed as follow: the extraction solvent was 20 mL 50% methanol, the ultrasound extraction time as 20 min. Under this condition, the linear range, instrument precision, solution stability, repeatability, accuracy(recovery) and durability of the HPLC method were investigated. The result indicated that HPLC was effective for determinationof gallic acid content in traditional Chinese gall leaven.
Gallic acid is one of main ingredients of traditional Chinese gall leaven. This study was conducted to develop a method for determination of gallic acid content in traditional Chinese gall leaven by HPLC. The effects of different extraction solvent, extraction volume, extraction time and extraction timeson gallic acid extraction were investigated and the optimal extraction condition was confirmed as follow: the extraction solvent was 20 mL 50% methanol, the ultrasound extraction time as 20 min. Under this condition, the linear range, instrument precision, solution stability, repeatability, accuracy(recovery) and durability of the HPLC method were investigated. The result indicated that HPLC was effective for determinationof gallic acid content in traditional Chinese gall leaven.
引文
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[3]王瑞生,张振凌,王胜超,等. HPLC法同时测定五倍子发酵百药煎中没食子酸及鞣花酸含量[J].中国现代中药,2016,18(7):831-845.
[4]刘起中,张可可. HPLC测定五倍子中没食子酸的含量[J].中草药,2002,33(5):427.
[5]叶定江.中药炮制学[M].北京:人民卫生出版社,1999:743-744.
[6]张婷,李满秀,贾海清,等. LC-MS/MS法测定大棚蔬菜氨基甲酸酯类农药残留分析研究[J].海南师范大学学报(自然科学版),2016,29(4):407-409.
[7]李志伟,梁丹,张建夫.氨基甲酸酯类农药残留分析方法的研究进展[J].华中农业大学学报,2008,27(5):691-695.
[8]李崇英,白亚之,钮松召,等.食品中氨基甲酸酯类农药残留的分析方法[J].分析科学学报,2007,23(6):723-724.