右美托咪定对糖尿病大鼠体液免疫及补体的影响
摘要
目的评价右美托咪定对糖尿病大鼠体液免疫及补体的影响。方法健康成年雄性SD大鼠72只,体重300~350 g,采用随机数字表法,将其随机分为3组(n=24):对照组(C组)、糖尿病组(DM组)和右美托咪定+糖尿病组(DEX组)。DM组和DEX组以链脲佐菌素(STZ)60 mg/kg一次性腹腔注射建立糖尿病大鼠模型,于模型稳定后4 w时3组大鼠均实施剖腹探查术,手术时间30 min。DEX组于手术开始前经10 min静脉输注右美托咪定负荷剂量1μg/kg,随后以5μg·kg~(-1)·h~(-1)(容量1 ml/h)的速率维持4 h,C组和DM组给予等容量生理盐水。分别于术前(T0)、术毕(T1)、术后24 h(T2)和72 h(T3)时每组随机取6只大鼠采集血样,测定血糖,并采用ELISA法检测血清Ig G、Ig A及Ig M和补体CH50、C3及C4浓度;分别于T0~T3时采血结束后处死大鼠,处死前称重,解剖分离脾脏组织称重,计算脾脏指数。结果与C组比较,DM组和DEX组T0~T3时血清Ig G、Ig M浓度及脾脏指数降低,Ig A、CH50、C3及C4浓度升高(P<0.05);与DM组比较,DEX组T1~T3时血清Ig G、Ig M浓度及脾脏指数升高,Ig A、CH50、C3及C4浓度降低(P<0.05)。结论右美托咪定可改善糖尿病大鼠围术期体液免疫及补体功能。
引文
1徐键,李树云,瞿秋,等.慢性阻塞性肺疾病合并2型糖尿病患者肺功能的改变〔J〕.中国老年学杂志,2013;33(6):1432-3.
2 MeretskyǐVM.The humoral immunity changes in experimental craniocerebral trauma,concurrent with diabetes mellitus〔J〕.Klin Khir,2013;(5):80-2.
3 Takeda Y,Shimomura T,Wakabayashi I.Immunological disorders of diabetes mellitus in experimental rat models〔J〕.Nihon Eiseigaku Zasshi,2014;69(3):166-7.
4 Gerlach AT,Murphy CV,Dasta JF.An updated focused review of dexmedetomidine in adults〔J〕.Ann Pharmacother,2009;43(12):2064-74.
5 梁桦,刘洪珍,王汉兵,等.右美托咪定对结肠癌根治术病人围术期细胞免疫功能和血循环微转移的影响〔J〕.中华麻醉学杂志,2012;32(10):1165-8.
6 高项羽,李姗,吕志峰.右美托咪定对糖尿病患者围术期糖代谢的影响〔J〕.河南外科学杂志,2013;19(4):76-7.
7 陈慰峰.医学免疫学〔M〕.北京:人民卫生出版社,2002:23.
8 Thumser AE,Moore JB,Plant NJ.Fatty acid binding proteins:tissue-specific functions in health and disease〔J〕.Curr Opin Clin Nutr Metab Care,2014;17(2):124-9.
9 Uesugi N,Sakata N,Nangaku M,et al.Possible mechanism for medial smooth muscle cell injury in diabetic nephropathy:glycoxidation mediated local complement activation〔J〕.Am J Kidney Dis,2004;44(2):224-38.
10 Nie F,Su D,Shi Y,et al.A preliminary study on the role of the complement regulatory protein,cluster of differentiation 55,in mice with diabetic neuropathic pain〔J〕.Mol Med Rep,2015;11(3):2076-82.
11 Hess K,Alzahrani SH,Price JF,et al.Hypofibrinolysis in type 2 diabetes:the role of the inflammatory pathway and complement C3〔J〕.Diabetologia,2014;57(8):1737-41.
12 付作文,程朝晖,谭海燕.右美托咪定对脑肿瘤切除术患者血清补体C3、C4水平的影响〔J〕.实用医学杂志,2013;29(8):1341-2.
13 张宏,于德民,陈樱,等.2型糖尿病患者免疫功能变化与血糖和胰岛素水平相关性的研究〔J〕.免疫检验和临床医学杂志,2002;9(2):90.
2 MeretskyǐVM.The humoral immunity changes in experimental craniocerebral trauma,concurrent with diabetes mellitus〔J〕.Klin Khir,2013;(5):80-2.
3 Takeda Y,Shimomura T,Wakabayashi I.Immunological disorders of diabetes mellitus in experimental rat models〔J〕.Nihon Eiseigaku Zasshi,2014;69(3):166-7.
4 Gerlach AT,Murphy CV,Dasta JF.An updated focused review of dexmedetomidine in adults〔J〕.Ann Pharmacother,2009;43(12):2064-74.
5 梁桦,刘洪珍,王汉兵,等.右美托咪定对结肠癌根治术病人围术期细胞免疫功能和血循环微转移的影响〔J〕.中华麻醉学杂志,2012;32(10):1165-8.
6 高项羽,李姗,吕志峰.右美托咪定对糖尿病患者围术期糖代谢的影响〔J〕.河南外科学杂志,2013;19(4):76-7.
7 陈慰峰.医学免疫学〔M〕.北京:人民卫生出版社,2002:23.
8 Thumser AE,Moore JB,Plant NJ.Fatty acid binding proteins:tissue-specific functions in health and disease〔J〕.Curr Opin Clin Nutr Metab Care,2014;17(2):124-9.
9 Uesugi N,Sakata N,Nangaku M,et al.Possible mechanism for medial smooth muscle cell injury in diabetic nephropathy:glycoxidation mediated local complement activation〔J〕.Am J Kidney Dis,2004;44(2):224-38.
10 Nie F,Su D,Shi Y,et al.A preliminary study on the role of the complement regulatory protein,cluster of differentiation 55,in mice with diabetic neuropathic pain〔J〕.Mol Med Rep,2015;11(3):2076-82.
11 Hess K,Alzahrani SH,Price JF,et al.Hypofibrinolysis in type 2 diabetes:the role of the inflammatory pathway and complement C3〔J〕.Diabetologia,2014;57(8):1737-41.
12 付作文,程朝晖,谭海燕.右美托咪定对脑肿瘤切除术患者血清补体C3、C4水平的影响〔J〕.实用医学杂志,2013;29(8):1341-2.
13 张宏,于德民,陈樱,等.2型糖尿病患者免疫功能变化与血糖和胰岛素水平相关性的研究〔J〕.免疫检验和临床医学杂志,2002;9(2):90.