益气养阴活血方对糖尿病肾病大鼠肾组织Nestin的影响
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摘要
目的:探讨益气养阴活血方对糖尿病肾病(DKD)模型大鼠肾组织Nestin表达的影响。方法:将36只SD雄性大鼠随机分为正常组、模型组、益气养阴活血方(中药)组各12只,模型组与中药组造模成功后,正常组和模型组灌服生理盐水,中药组给予益气养阴活血方颗粒灌胃。6周后生化检测各组大鼠血清白蛋白(Alb)、丙氨酸氨基转移酶(ALT)、血肌酐(Scr)、血尿素氮(BUN)、24 h尿蛋白定量、空腹血糖(FBG)及血脂(TC、TG)等水平;测量大鼠体重、肾质量,计算肥大指数;过碘酸-雪夫氏(PAS)染色观察肾脏外观及病理;免疫组化法检测肾组织Nestin的表达水平。结果:益气养阴活血方可以改善大鼠基本状况及生化指标,减少蛋白尿、改善肾脏病理,肾质量及肥大指数降低,并且发现Nestin在肾组织的表达变化与上述生化及病理改善明显相关。结论:益气养阴活血方可能通过上调Nestin达到保护足细胞、降低蛋白尿及改善肾脏病理而延缓DKD进程。
Objective: To investigate the effect of Yiqi Yangyin Huoxue recipe on Nestin expression in renal tissue of diabetic nephropathy( DKD) rats. Methods: Thirty-six male SD rats were randomly divided into normal group,model group,Yiqi Yangyin Huoxue prescription group( 12 rats),model group and traditional Chinese medicine group were successfully established,normal group and model group were given normal saline. After 6 weeks,serum albumin( Alb),alanine aminotransferase( ALT),serum creatinine( Scr),blood urea nitrogen( BUN),24 hours urine protein were measured. Fasting blood glucose( FBG) and blood lipid( TC,TG) levels; Body weight,kidney mass and hypertrophy index were calculated. The appearance and pathology of kidney were observed by( PAS) staining. The expression of Nestin in renal tissue was detected by immunohistochemistry. Results: Yiqi Yangyin Huoxue prescription could improve the basic condition and biochemical index of rats,reduce proteinuria,improve renal pathology,decrease renal quality and hypertrophy index. It was also found that the expression of Nestin in renal tissue was significantly correlated with the biochemical and pathological changes mentioned above. Conclusion: Yiqi yangyin huoxue prescription may delay the progress of DKD by upregulating Nestin to protect podocytes,reduce proteinuria and improve renal pathology.
Objective: To investigate the effect of Yiqi Yangyin Huoxue recipe on Nestin expression in renal tissue of diabetic nephropathy( DKD) rats. Methods: Thirty-six male SD rats were randomly divided into normal group,model group,Yiqi Yangyin Huoxue prescription group( 12 rats),model group and traditional Chinese medicine group were successfully established,normal group and model group were given normal saline. After 6 weeks,serum albumin( Alb),alanine aminotransferase( ALT),serum creatinine( Scr),blood urea nitrogen( BUN),24 hours urine protein were measured. Fasting blood glucose( FBG) and blood lipid( TC,TG) levels; Body weight,kidney mass and hypertrophy index were calculated. The appearance and pathology of kidney were observed by( PAS) staining. The expression of Nestin in renal tissue was detected by immunohistochemistry. Results: Yiqi Yangyin Huoxue prescription could improve the basic condition and biochemical index of rats,reduce proteinuria,improve renal pathology,decrease renal quality and hypertrophy index. It was also found that the expression of Nestin in renal tissue was significantly correlated with the biochemical and pathological changes mentioned above. Conclusion: Yiqi yangyin huoxue prescription may delay the progress of DKD by upregulating Nestin to protect podocytes,reduce proteinuria and improve renal pathology.
引文
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2.曾洁,谢席胜,冯胜刚.活性维生素D3对高糖环境下小鼠足细胞巢蛋白表达的影响.中国中西医结合肾病杂志,2016,17(10):899-901.
3.宋纯东,喻青,吴晨晨.益气养阴活血方对糖尿病肾病大鼠肾组织TRPC6、Rho A表达的影响.中华中医药学刊,2017,35(11):2738-2740.
4.宋纯东,张霞,宋丹丹,等.益气养阴活血方对糖尿病肾病大鼠肾组织WT1影响的实验研究.中国中医基础医学杂志,2016,22(05):626-627,629.
5.宋纯东,张绿凤,秦林芳,等.益肾活血方联合缬沙坦对糖尿病肾病大鼠肾组织Rho A/ROCK1表达的影响.中国中医基础医学杂志,2016,22(2):198-199,251.
6.薛黎明,宋纯东,张绿风,等.益气养阴活血方联合雷公藤多苷片治疗糖尿病肾病Ⅳ期临床研究.新中医,2017,49(9):48-50.
7.贾评评,宋丹,宋纯东,等.六味地黄丸对糖尿病肾病大鼠肾脏组织Rho A/ROCK1表达的影响.中医学报,2018,33(9):1636-1640.
8.孟加宁,姚源璋.高糖环境下糖尿病肾病蛋白尿的发病机制.中国中西医结合肾病杂志,2016,17(12):1121-1123.
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10.王娜,魏日胞.足细胞裂孔隔膜相关蛋白分子研究进展.中国中西医结合肾病杂志,2016,17(3):266-268.
11.杨汝春,朱晓玲,张华琴,等.白藜芦醇对TGF-β1诱导的足细胞凋亡及骨架分子损害的干预作用.中国中西医结合肾病杂志,2013,14(1):9-12.
12.Schell C,Huber TB.The Evolving Complexity of the Podocyte Cytoskeleton.J Am Soc Nephrol,2017,28(11):3166-3174.
13.汪建成.中间丝蛋白Nestin调节细胞内环境稳态的作用机理研究.中山大学,2016.
14.陶花,顾乐怡.足细胞骨架相关蛋白的调节及其在细胞生物学中的作用.中国中西医结合肾病杂志,2011,12(7):641-645.
15.Liu W,Zhang Y,Hao J,et al.Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism.J Cell Biochem,2014,115(7):3186-3196.
16.杨敏,封晓娟,吴超,等.狼疮性肾炎肾小球中nestin和活化型Caspase3的表达及意义.临床与实验病理学杂志,2016,32(2):203-206.
17.秦林芳.益气养阴活血方对糖尿病肾病大鼠肾组织Nephrin、Nestin、Desmin影响的实验研究.河南中医学院,2015.
18.路建饶,陈晛,陈杰.传统医学对糖尿病肾病发病机制的研究进展.时珍国医国药,2018,29(02):415-417.
19.张向伟,柳红芳,张先慧.糖尿病肾病病机层次分析与辨治.中医杂志,2017,58(5):390-393.
2.曾洁,谢席胜,冯胜刚.活性维生素D3对高糖环境下小鼠足细胞巢蛋白表达的影响.中国中西医结合肾病杂志,2016,17(10):899-901.
3.宋纯东,喻青,吴晨晨.益气养阴活血方对糖尿病肾病大鼠肾组织TRPC6、Rho A表达的影响.中华中医药学刊,2017,35(11):2738-2740.
4.宋纯东,张霞,宋丹丹,等.益气养阴活血方对糖尿病肾病大鼠肾组织WT1影响的实验研究.中国中医基础医学杂志,2016,22(05):626-627,629.
5.宋纯东,张绿凤,秦林芳,等.益肾活血方联合缬沙坦对糖尿病肾病大鼠肾组织Rho A/ROCK1表达的影响.中国中医基础医学杂志,2016,22(2):198-199,251.
6.薛黎明,宋纯东,张绿风,等.益气养阴活血方联合雷公藤多苷片治疗糖尿病肾病Ⅳ期临床研究.新中医,2017,49(9):48-50.
7.贾评评,宋丹,宋纯东,等.六味地黄丸对糖尿病肾病大鼠肾脏组织Rho A/ROCK1表达的影响.中医学报,2018,33(9):1636-1640.
8.孟加宁,姚源璋.高糖环境下糖尿病肾病蛋白尿的发病机制.中国中西医结合肾病杂志,2016,17(12):1121-1123.
9.Crl C,Leite NC,Salles GC,et al.Aortic stiffness and ambulatory blood pressure as predictors of diabetic kidney disease:a competing risks analysis from the Rio de Janeiro Type 2 Diabetes Cohort Study.Diabetologia,2018,61(2):455-465.
10.王娜,魏日胞.足细胞裂孔隔膜相关蛋白分子研究进展.中国中西医结合肾病杂志,2016,17(3):266-268.
11.杨汝春,朱晓玲,张华琴,等.白藜芦醇对TGF-β1诱导的足细胞凋亡及骨架分子损害的干预作用.中国中西医结合肾病杂志,2013,14(1):9-12.
12.Schell C,Huber TB.The Evolving Complexity of the Podocyte Cytoskeleton.J Am Soc Nephrol,2017,28(11):3166-3174.
13.汪建成.中间丝蛋白Nestin调节细胞内环境稳态的作用机理研究.中山大学,2016.
14.陶花,顾乐怡.足细胞骨架相关蛋白的调节及其在细胞生物学中的作用.中国中西医结合肾病杂志,2011,12(7):641-645.
15.Liu W,Zhang Y,Hao J,et al.Nestin protects mouse podocytes against high glucose-induced apoptosis by a Cdk5-dependent mechanism.J Cell Biochem,2014,115(7):3186-3196.
16.杨敏,封晓娟,吴超,等.狼疮性肾炎肾小球中nestin和活化型Caspase3的表达及意义.临床与实验病理学杂志,2016,32(2):203-206.
17.秦林芳.益气养阴活血方对糖尿病肾病大鼠肾组织Nephrin、Nestin、Desmin影响的实验研究.河南中医学院,2015.
18.路建饶,陈晛,陈杰.传统医学对糖尿病肾病发病机制的研究进展.时珍国医国药,2018,29(02):415-417.
19.张向伟,柳红芳,张先慧.糖尿病肾病病机层次分析与辨治.中医杂志,2017,58(5):390-393.