抑癌基因TCEAL7在乳腺组织中的表达及与人端粒酶逆转录酶的关系
摘要
目的探讨抑癌基因TCEAL7在乳腺组织中的表达特征及其与人端粒酶逆转录酶(h TERT)间的相互关系。方法运用RT-PCR技术检测乳腺良性组织及乳腺癌组织中TCEAL7、h TERT的表达状况,收集对应患者的临床及基本病理资料[包括年龄、淋巴结转移状态、TNM分期及免疫组化结果(ER、PR及HER2表达情况)],利用统计学方法分析讨论它们之间的相互关系。结果 TCEAL7基因在14例乳腺良性组织中全部表达,但在55例乳腺癌组织中却没有发现表达;h TERT在乳腺良性组织中有1例表达,在乳腺癌组织中有40例表达。h TERT(+)及APBs(+)组之间年龄、肿瘤大小、淋巴结转移及病理分期均差异无统计学意义。在分析h TERT与临床基本资料时,发现其与临床分期相关。结论抑癌基因TCEAL7在乳腺正常组织中高表达,在乳腺癌组织中几乎没有表达。在细胞水平,TCEAL7基因在端粒延长替代机制细胞中高表达,而在端粒酶阳性细胞中低表达,并且促进h TERT的转录,发现h TERT表达与肿瘤的病理分期相关。
引文
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[2]Rattan R,Narita K,Chien J,et al.TCEAL7,a putative tumor suppressor gene,negatively regulates NF-kappa B pathway[J].Oncogene,2010,29(9):1362-1373.
[3]Telli ML,Ford JM.Novel treatment approaches for triple-negative breast cancer[J].Clin Breast Cancer,2010,10 Suppl 1:E16-E22.
[4]Mccloskey SA,Lee SP,Steinberg ML.Roles and types of radiation in breast cancer treatment:early breast cancer,locoregionally advanced,and metastatic disease[J].Curr Opin Obstet Gynecol,2011,23(1):51-57.
[5]Greenberg S,Stopeck A,Rugo HS.Systemic treatment of early breast cancer——a biological perspective[J].J Surg Oncol,2011,103(6):619-626.
[6]Caudle AS,Hunt KK.The neoadjuvant approach in breast cancer treatment:it is not just about chemotherapy anymore[J].Curr Opin Obstet Gynecol,2011,23(1):31-36.
[7]Agrawal AK,Jelen M,Rudnicki J,et al.Molecular markers(cerb B-2,p53)in breast cancer[J].Folia Histochem Cytobiol,2008,46(4):449-455.
[8]Shridhar V,Sen A,Chien J,et al.Identification of underexpressed genes in early-and late-stage primary ovarian tumors by suppression subtraction hybridization[J].Cancer Res,2002,62(1):262-270.
[9]Chien J,Narita K,Rattan R,et al.A role for candidate tumorsuppressor gene TCEAL7 in the regulation of c-Myc activity,cyclin D1 levels and cellular transformation[J].Oncogene,2008,27(58):7223-7234.
[10]Chien J,Staub J,Avula R,et al.Epigenetic silencing of TCEAL7(Bex4)in ovarian cancer[J].Oncogene,2005,24(32):5089-5100.
[11]Hashimoto Y,Murakami Y,Uemura K,et al.Detection of human telomerase reverse transcriptase(h TERT)expression in tissue and pancreatic juice from pancreatic cancer[J].Surgery,2008,143(1):113-125.
[12]Qi DL,Ohhira T,Oshimura M,et al.Human chromosome 5 carries a transcriptional regulator of human telomerase reverse transcriptase(h TERT)[J].Biochem Biophys Res Commun,2010,398(4):695-701.
[13]Rao YK,Kao TY,Wu MF,et al.Identification of small molecule inhibitors of telomerase activity through transcriptional regulation of h TERT and calcium induction pathway in human lung adenocarcinoma A549 cells[J].Bioorg Med Chem,2010,18(19):6987-6994.