中草药复方多糖对肉鸡肠道菌群及免疫功能的影响
|
摘要
为探讨中草药复方多糖(CHCP)对鸡肠道菌群、新城疫(ND-HI)抗体效价及血清补体C3、C4含量的影响,试验选用200羽1日龄商品代AA雏肉鸡,随机分为4组,每组50羽,于7、28 d进行ND疫苗免疫,对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组在基础日粮中分别添加200、400、600 mg/kg的CHCP,试验期为42 d。结果表明:试验Ⅰ、Ⅱ、Ⅲ组的鸡盲肠内双歧杆菌分别较对照组提高3.19%(P> 0.05)、9.79%、6.82%(P <0.05),乳酸杆菌分别提高8.08%(P <0.05)、17.45%、16.86%(P <0.01);Ⅱ、Ⅲ组大肠杆菌和沙门氏菌的数量分别较对照组降低14.34%、12.15%和16.19%、16.61%(P <0.01);14 d时,Ⅰ、Ⅱ、Ⅲ组的ND-HI抗体效价与对照组相比分别提高9.80%、17.32%、13.56%(P <0.05),28、42 d时,Ⅱ、Ⅲ组效果最为显著,分别比对照组提高36.12%、25.79%和35.98%、30.18%(P <0.01);血清中补体C3、C4的含量与对照组相比均显著提高(P <0.05)。综上所述,在鸡的日粮中添加一定量的CHCP可促进有益菌的增殖,改善肉鸡肠道内环境,提高鸡的免疫功能。且以400 mg/kg和600 mg/kg的量添加效果最好,从经济角度考虑,400 mg/kg CHCP为最佳添加量。
To investigate the effect of Chinese herbal compound polysaccharide(CHCP) on chicken intestinal flora, Newcastle disease(ND) antibody titer and serum C3 and C4 levels, 200 1-day-old of AA broiler chickens were randomly divided into 4 groups of 50 chickens each.Immunization with ND vaccine was carried out in 7 d and 28 d, the chickens in the control group were fed basal diet,and groups Ⅰ, Ⅱ, Ⅲ were added 200,400,600 mg/kg CHCP in the basal diet. The test period was 42 d.The results showed that the Bifidobacteria in cecum of chickens in groups Ⅰ,Ⅱ,Ⅲ were increased by 3.19%,9.79%,6.82%,respectively(P < 0.05), while Lactobacillus were increased by 8.08%(P < 0.05),17.45% and 16.86%, respectively(P <0.01);The number of Escherichia coli and Salmonella in groups II and III were decreased by 14.34%,12.15%and 16.19%,16.61%,respectively(P < 0.01);At 14 d, the ND antibody titers of groups Ⅰ, Ⅱ, Ⅲ were increased by 9.80%, 17.32%, 13.56%, respectively,compared with the control group(P < 0.05), at 28 d and42 d,the effects of groups II and III were the nost significant,which were 36.12%, 25.79% and 35.98%,30.18% higher than that of the control group(P < 0.01);The contents of complement C3 and C4 in serum were significantly higher than those in the control group(P < 0.05).To sum up,adding a certain amount of CHCP to the daily diet of chickens could promote the proliferation of beneficial bacteria,improve the intestinal environment and immune function of broiler chickens. Moreover, 400 mg/kg and 600 mg/kg were the most effective dosages.From economic perspective, 400 mg/kg was the best dosages.
To investigate the effect of Chinese herbal compound polysaccharide(CHCP) on chicken intestinal flora, Newcastle disease(ND) antibody titer and serum C3 and C4 levels, 200 1-day-old of AA broiler chickens were randomly divided into 4 groups of 50 chickens each.Immunization with ND vaccine was carried out in 7 d and 28 d, the chickens in the control group were fed basal diet,and groups Ⅰ, Ⅱ, Ⅲ were added 200,400,600 mg/kg CHCP in the basal diet. The test period was 42 d.The results showed that the Bifidobacteria in cecum of chickens in groups Ⅰ,Ⅱ,Ⅲ were increased by 3.19%,9.79%,6.82%,respectively(P < 0.05), while Lactobacillus were increased by 8.08%(P < 0.05),17.45% and 16.86%, respectively(P <0.01);The number of Escherichia coli and Salmonella in groups II and III were decreased by 14.34%,12.15%and 16.19%,16.61%,respectively(P < 0.01);At 14 d, the ND antibody titers of groups Ⅰ, Ⅱ, Ⅲ were increased by 9.80%, 17.32%, 13.56%, respectively,compared with the control group(P < 0.05), at 28 d and42 d,the effects of groups II and III were the nost significant,which were 36.12%, 25.79% and 35.98%,30.18% higher than that of the control group(P < 0.01);The contents of complement C3 and C4 in serum were significantly higher than those in the control group(P < 0.05).To sum up,adding a certain amount of CHCP to the daily diet of chickens could promote the proliferation of beneficial bacteria,improve the intestinal environment and immune function of broiler chickens. Moreover, 400 mg/kg and 600 mg/kg were the most effective dosages.From economic perspective, 400 mg/kg was the best dosages.
引文
[1]段智璇,田维毅.中草药对肠道菌群及肠黏膜屏障功能的影响[J].内蒙古医学杂志,2016,48(9):1053~1057.
[2]方磊涵,毕玉霞,王振,等.绞股蓝多糖对猪瘟弱毒疫苗免疫效果的影响[J].中兽医医药杂志,2018,37(1):58~60.
[3]郝桂英,钟元良.黄芪多糖对新城疫弱毒疫苗免疫雏鸡血凝抑制抗体水平的影响[J].西昌学院学报.自然科学版,2013,27(1):14~16.
[4]黄皓,郝丽,肖向红,等.肠道菌群功能及检测技术研究进展[J].黑龙江畜牧兽医,2018,13:45~48.
[5]黄志坚,谢红兵,陈强,等.精制中草药免疫增强剂对断奶仔猪免疫功能的影响[J].福建农林大学学报(自然科学版),2007,36(2):163~166.
[6]靳录洋,李星艳.中草药多糖对雏鸡淋巴细胞转化和新城疫抗体水平的影响[J].畜牧兽医杂志,2009,28(3):7~10.
[7]雷红,祁成年,郭世宁.几种中草药方剂对鸡血清免疫球蛋白和补体的影响[J].中国兽医杂志,2007,1:33~34.
[8]李杰,田博,徐良梅,等.不同类型女贞子对AA肉鸡生产性能及免疫功能的影响[J].中国畜牧杂志,2011,47(23):58~62.
[9]李平兰,吕燕妮,郑海涛,等.中草药对鸡肠道微生物菌群的影响[J].饲料研究,2003,5:1~3.
[10]李清丰,赵川,黄光远,等."激饮康"对夏季肉兔血清兔瘟抗体及补体C3、C4含量的影响[J].黑龙江畜牧兽医,2018,6:177~179.
[11]刘静,朱盛山,罗裕,等.中草药在养鸡业中的应用概述[J].中兽医学杂志,2013,49(6):49~52.
[12]孙波,陈静,吴娟,等.黄芪多糖对免疫抑制鸡生长性能、肠道菌群及免疫功能的影响[J].中国畜牧兽医,2014,41(8):101~106.
[13]王俊丽,章世元,徐春燕,等.黄芪多糖对肉仔鸡生产性能和部分免疫指标的影响[J].中国饲料,2010,7:18~21.
[14]王留,王向国.大枣多糖对保育猪肠道微生物菌群及肠道组织形态的影响[J].养猪,2014,6:7
[15]魏炳栋,陈群,于维,等.黄芪多糖对三黄肉鸡生长性能及肠道菌群的影响[J].中国饲料,2013,17:26~28.
[16]肖啸,张开伟,沈学文,等.黄芪多糖对犬免疫指标的影响[J].山东畜牧兽医,2009,30(11):3~5.
[17]杨红洋,朱晓庆,谷新利.纯化后中草药多糖对鸡新城疫疫苗免疫、T淋巴细胞转化率及IgG、IgM的影响[J].石河子大学学报,2017,35(2):152~156.
[18]杨玉红,张帆.中草药多糖的生物学活性及其在畜牧兽医中的应用[J].中国兽医杂志,2013,9:94~96.
[19]于红梅.淫羊藿多糖提取工艺的优化及其对肉鸡免疫功能的调节作用:[硕士学位论文][D].聊城:聊城大学,2015.
[20]尹瑞卿.枸杞多糖对肉鸡生产性能和免疫功能的调节作用:[硕士学位论文][D].杨凌:西北农林科技大学,2013.
[21]赵国先,邵丽玮,冯志华,等.刺五加多糖对蛋雏鸡生长性能及血清免疫指标的影响[J].饲料研究,2016,17:15~19.
[22]赵娟,贺东昌,武果桃,等.中草药复方颗粒剂对肉仔鸡肠道菌群的影响[J].中国兽医杂志,2011,47(6):48~49.
[2]方磊涵,毕玉霞,王振,等.绞股蓝多糖对猪瘟弱毒疫苗免疫效果的影响[J].中兽医医药杂志,2018,37(1):58~60.
[3]郝桂英,钟元良.黄芪多糖对新城疫弱毒疫苗免疫雏鸡血凝抑制抗体水平的影响[J].西昌学院学报.自然科学版,2013,27(1):14~16.
[4]黄皓,郝丽,肖向红,等.肠道菌群功能及检测技术研究进展[J].黑龙江畜牧兽医,2018,13:45~48.
[5]黄志坚,谢红兵,陈强,等.精制中草药免疫增强剂对断奶仔猪免疫功能的影响[J].福建农林大学学报(自然科学版),2007,36(2):163~166.
[6]靳录洋,李星艳.中草药多糖对雏鸡淋巴细胞转化和新城疫抗体水平的影响[J].畜牧兽医杂志,2009,28(3):7~10.
[7]雷红,祁成年,郭世宁.几种中草药方剂对鸡血清免疫球蛋白和补体的影响[J].中国兽医杂志,2007,1:33~34.
[8]李杰,田博,徐良梅,等.不同类型女贞子对AA肉鸡生产性能及免疫功能的影响[J].中国畜牧杂志,2011,47(23):58~62.
[9]李平兰,吕燕妮,郑海涛,等.中草药对鸡肠道微生物菌群的影响[J].饲料研究,2003,5:1~3.
[10]李清丰,赵川,黄光远,等."激饮康"对夏季肉兔血清兔瘟抗体及补体C3、C4含量的影响[J].黑龙江畜牧兽医,2018,6:177~179.
[11]刘静,朱盛山,罗裕,等.中草药在养鸡业中的应用概述[J].中兽医学杂志,2013,49(6):49~52.
[12]孙波,陈静,吴娟,等.黄芪多糖对免疫抑制鸡生长性能、肠道菌群及免疫功能的影响[J].中国畜牧兽医,2014,41(8):101~106.
[13]王俊丽,章世元,徐春燕,等.黄芪多糖对肉仔鸡生产性能和部分免疫指标的影响[J].中国饲料,2010,7:18~21.
[14]王留,王向国.大枣多糖对保育猪肠道微生物菌群及肠道组织形态的影响[J].养猪,2014,6:7
[15]魏炳栋,陈群,于维,等.黄芪多糖对三黄肉鸡生长性能及肠道菌群的影响[J].中国饲料,2013,17:26~28.
[16]肖啸,张开伟,沈学文,等.黄芪多糖对犬免疫指标的影响[J].山东畜牧兽医,2009,30(11):3~5.
[17]杨红洋,朱晓庆,谷新利.纯化后中草药多糖对鸡新城疫疫苗免疫、T淋巴细胞转化率及IgG、IgM的影响[J].石河子大学学报,2017,35(2):152~156.
[18]杨玉红,张帆.中草药多糖的生物学活性及其在畜牧兽医中的应用[J].中国兽医杂志,2013,9:94~96.
[19]于红梅.淫羊藿多糖提取工艺的优化及其对肉鸡免疫功能的调节作用:[硕士学位论文][D].聊城:聊城大学,2015.
[20]尹瑞卿.枸杞多糖对肉鸡生产性能和免疫功能的调节作用:[硕士学位论文][D].杨凌:西北农林科技大学,2013.
[21]赵国先,邵丽玮,冯志华,等.刺五加多糖对蛋雏鸡生长性能及血清免疫指标的影响[J].饲料研究,2016,17:15~19.
[22]赵娟,贺东昌,武果桃,等.中草药复方颗粒剂对肉仔鸡肠道菌群的影响[J].中国兽医杂志,2011,47(6):48~49.