屈头鸡果预灌胃对哮喘小鼠气道Th1/Th2型细胞因子水平的调节作用
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摘要
目的观察屈头鸡果预灌胃对哮喘小鼠气道Th1/Th2型细胞因子水平的调节作用。方法将50只BALB/c小鼠随机分为正常对照组(A组)、哮喘模型组(B组)、屈头鸡果治疗剂量组(C组)、屈头鸡果中毒剂量组(D组)、地塞米松治疗组(E组) 5组,每组10只。B组第0、14天腹腔注射0. 2 m L致敏液,第24天以1%卵蛋白溶液10 m L雾化激发哮喘,每天30 min,连续28天; A组代之等量生理盐水; C、D组以上述同样方法用卵蛋白进行致敏和激发小鼠,在每次雾化激发哮喘前30 min分别灌胃屈头鸡果果浆2. 16、10. 8 g/kg,E组腹腔注射地塞米松2mg/kg。在末次激发结束后采集各组小鼠肺泡灌洗液,以ELISA法测定各组小鼠肺泡灌洗液Th1型细胞因子IL-2、IL-10、IFN-γ以及Th2型细胞因子IL-4、IL-5的水平。结果末次激发结束时A组、B组、C组及E组小鼠均存活,D组小鼠在给药的过程中先后死亡4只,存活的6只也存在不同程度的呕吐、腹泻、心律加快和抽搐等症状。与A组比较,B、C、D、E组小鼠肺泡灌洗液IL-2、IL-10、IFN-γ水平低,IL-4、IL-5水平高(P均<0. 01)。与B组比较,C、D、E组小鼠肺泡灌洗液IL-2、IL-10、IFN-γ水平高,IL-4、IL-5水平低(P均<0. 05)。结论屈头鸡果预灌胃对哮喘小鼠气道IL-4、IL-5等Th2型细胞因子水平具有抑制作用,并可激活IL-2、IL-10、IFN-γ等Th1型细胞因子,具有抑制哮喘气道炎症的作用;但屈头鸡果具有一定毒性。
Objective To observe the regulatory effect of Capparis versicolor Griff on airway type Th1/Th2 cytokines in asthmatic mice. Methods Fifty BALB/c mice were randomly divided into five groups: normal control group( group A),asthma model group( group B),Capparis versicolor Griff therapeutic dose group( group C),Capparis versicolor Griff toxic dose group( group D),and dexamethasone treatment group( group E). Group B was administered 0. 2 m L sensitizer intraperitoneally on the 0 and 14 th day,and the asthma was stimulated by aerosol inhalation of 1% ovalbumin solution( 10 m L)on the 24 th day for 30 minutes every day,for 28 days; group A was replaced by the same amount of normal saline; groups C and D were sensitized and stimulated by ovalbumin in the same way,and the mice were given Capparis versicolor Griff2. 16 g/kg( group C) or 10. 8 g/kg( group D) 30 minutes before every atomization; group E was intraperitoneally injected with dexamethasone 2 mg/kg. After the last stimulation,alveolar lavage fluid was collected and the levels of Th1 cytokines interleukin-2( IL-2),IL-10,tumor necrosis factor-γ( IFN-γ),Th2 cytokines IL-4 and IL-5 in the alveolar lavage fluid were determined by enzyme linked immunosorbent assay( ELISA). Results At the end of the last stimulation,mice in the group A,group B,group C and group E all survived. Four mice in the group D died successively in the course of administration. Six survived mice also suffered from vomiting,diarrhea,tachycardia and convulsions. Compared with the group A,the levels of IL-2,IL-10 and IFN-γ in the alveolar lavage fluid were lower,and the levels of IL-4 and IL-5 were higher in the groups B,C,D and E( all P < 0. 01). Compared with the group B,the levels of IL-2,IL-10,and IFN-γwere higher,and the levels of IL-4 and IL-5 were lower in the groups C,D and E( all P < 0. 05). Conclusion The pretreatment of Capparis versicolor Griff can inhibit the levels of Th 2 cytokines such as IL-4 and IL-5 in the airway of asthmatic mice,and activate Th1 cytokines such as IL-2,IL-10 and IFN-gamma,which can inhibit the airway inflammation ofasthma,but Capparis versicolor Griff has certain toxicity.
Objective To observe the regulatory effect of Capparis versicolor Griff on airway type Th1/Th2 cytokines in asthmatic mice. Methods Fifty BALB/c mice were randomly divided into five groups: normal control group( group A),asthma model group( group B),Capparis versicolor Griff therapeutic dose group( group C),Capparis versicolor Griff toxic dose group( group D),and dexamethasone treatment group( group E). Group B was administered 0. 2 m L sensitizer intraperitoneally on the 0 and 14 th day,and the asthma was stimulated by aerosol inhalation of 1% ovalbumin solution( 10 m L)on the 24 th day for 30 minutes every day,for 28 days; group A was replaced by the same amount of normal saline; groups C and D were sensitized and stimulated by ovalbumin in the same way,and the mice were given Capparis versicolor Griff2. 16 g/kg( group C) or 10. 8 g/kg( group D) 30 minutes before every atomization; group E was intraperitoneally injected with dexamethasone 2 mg/kg. After the last stimulation,alveolar lavage fluid was collected and the levels of Th1 cytokines interleukin-2( IL-2),IL-10,tumor necrosis factor-γ( IFN-γ),Th2 cytokines IL-4 and IL-5 in the alveolar lavage fluid were determined by enzyme linked immunosorbent assay( ELISA). Results At the end of the last stimulation,mice in the group A,group B,group C and group E all survived. Four mice in the group D died successively in the course of administration. Six survived mice also suffered from vomiting,diarrhea,tachycardia and convulsions. Compared with the group A,the levels of IL-2,IL-10 and IFN-γ in the alveolar lavage fluid were lower,and the levels of IL-4 and IL-5 were higher in the groups B,C,D and E( all P < 0. 01). Compared with the group B,the levels of IL-2,IL-10,and IFN-γwere higher,and the levels of IL-4 and IL-5 were lower in the groups C,D and E( all P < 0. 05). Conclusion The pretreatment of Capparis versicolor Griff can inhibit the levels of Th 2 cytokines such as IL-4 and IL-5 in the airway of asthmatic mice,and activate Th1 cytokines such as IL-2,IL-10 and IFN-gamma,which can inhibit the airway inflammation ofasthma,but Capparis versicolor Griff has certain toxicity.
引文
[1]Nathan RA,Thompson PJ,Price D,et al. Taking Aim at Asthma Around the World:Global Results of the Asthma Insight and ManagementSurvey in the Asia-Pacific Region,Latin America,Europe,Canada,and the United States[J]. J Allergy Clin Immunol Pract,2015,3(5):734-742.
[2]《全国中草药汇编》编写组.全国中草药汇编[M].下册.北京:人民卫生出版社,1986:376.
[3]覃冠德,黄正志.屈头鸡果急性中毒34例救治分析[J].实用医学杂志,2012,28(6):1029.
[4]Rogala B,Bozek A,Gluck J,et al. Prevalence of Ig E-mediated allergy and evaluation of Th1/Th2 cytokine profiles in patients with severe bronchial asthma[J]. Postepy Dermatol Alergol,2015,32(4):274-280.
[5]Wegmann M. Th2 cells as targets for therapeutic intervention in allergic bronchial asthma[J]. Expert Rev Mol Diagn,2009,9(1):85-100.
[6] Walsh GM. Biologics targeting IL-5,IL-4 or IL-13 for the treatment of asthma-an update[J]. Expert Rev Clin Immunol,2017,13(2):143-149.
[7] Lam C,Shah KJ,Mansukhani R. Targeting interleukin-5 in patients with severe eosinophilic asthma:a clinical review[J]. P T,2017,42(3):196-201.
[8]Shi H,Qin S,Huang G,et al. Infiltration of eosinophils into the asthmatic airways caused by interleukin 5[J]. Am J Respir Cell Mol Biol,1997,16(3):220-224.
[9]Yim RP,Koumbourlis AC. Steroid-resistant asthma[J]. Paediatr Respir Rev,2012,13(3):172-176.
[10]Lefebvre P,Duh MS,Lafeuille MH,et al. Burden of systemic glucocorticoid-related complications in severe asthma[J]. Curr Med Res Opin,2017,33(1):57-65.
[2]《全国中草药汇编》编写组.全国中草药汇编[M].下册.北京:人民卫生出版社,1986:376.
[3]覃冠德,黄正志.屈头鸡果急性中毒34例救治分析[J].实用医学杂志,2012,28(6):1029.
[4]Rogala B,Bozek A,Gluck J,et al. Prevalence of Ig E-mediated allergy and evaluation of Th1/Th2 cytokine profiles in patients with severe bronchial asthma[J]. Postepy Dermatol Alergol,2015,32(4):274-280.
[5]Wegmann M. Th2 cells as targets for therapeutic intervention in allergic bronchial asthma[J]. Expert Rev Mol Diagn,2009,9(1):85-100.
[6] Walsh GM. Biologics targeting IL-5,IL-4 or IL-13 for the treatment of asthma-an update[J]. Expert Rev Clin Immunol,2017,13(2):143-149.
[7] Lam C,Shah KJ,Mansukhani R. Targeting interleukin-5 in patients with severe eosinophilic asthma:a clinical review[J]. P T,2017,42(3):196-201.
[8]Shi H,Qin S,Huang G,et al. Infiltration of eosinophils into the asthmatic airways caused by interleukin 5[J]. Am J Respir Cell Mol Biol,1997,16(3):220-224.
[9]Yim RP,Koumbourlis AC. Steroid-resistant asthma[J]. Paediatr Respir Rev,2012,13(3):172-176.
[10]Lefebvre P,Duh MS,Lafeuille MH,et al. Burden of systemic glucocorticoid-related complications in severe asthma[J]. Curr Med Res Opin,2017,33(1):57-65.