刺玫果总皂苷的纯化工艺及体外活性研究
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摘要
研究不同型号树脂对刺玫果总皂苷的纯化效果及刺玫果总皂苷体外活性.分别采用LSA-21、D-101和AB-8等8种型号的大孔树脂对刺玫果总皂苷进行纯化,通过紫外-可见分光光度法测定吸光度,从而计算吸附率和解吸率吸附率和解吸率.结果表明,LSA-21型大孔树脂对对刺玫果总皂苷的纯化效果最好,其最佳工艺条件为:吸附液浓度为0.192 7 mg/mL,吸附液体积为25 mL,吸附液pH为6.5,乙醇体积分数为90%,解吸液体积为40 mL,解吸液pH为8.5.在上述纯化条件下,刺玫果总皂苷的吸附率、解吸率分别可以达到77.51%和65.28%,干浸膏中总皂苷的含量由9.49%提高到33.65%.试验结果表明,刺玫果总皂苷对DPPH·、·OH、超氧阴离子自由基、ABTS自由基均具有清除能力,同时具有一定的体外抗氧化活性,并能够抑制脂质和α-葡萄糖苷酶的活性,试验结果为刺玫果总皂苷的进一步研究提供了参考.
The purification effect of different models resin and their activity in vitro of the total saponins in fruit of Rosa davuvrica Pall.were studied.Eight types of macroporous resins,such as LSA-21,D-101 and AB-8,were used to purify the total saponins from Fruit of Rosa davuvrica Pall. Adsorption rate and desorption rate were determinated by ultraviolet-visible spectrophotometry method. The results showed that the LSA-21 type of macroporous resin had the best purification effect on the total saponins in fruit of Rosa davuvrica Pall.The best conditions were as follows: The concentration of adsorption liquid was 0.192 7 g/mL.The volume of adsorption liquid was 25 mL.The pH of adsorption liquid was 6.5.The volume fraction of ethanol was 90%.The volume of desorption liquid was 40 mL and the desorption solution pH was 8.5.With the above purification conditions,the calculated adsorption and desorption rate of the total saponins in fruit of Rosa davuvrica Pall. could reach77.51% and 65.28%,respectively.The content of the total saponins in dry extract was increased from 9.49% to33.65%.The test results showed that scavenging ability to DPPH·,·OH,superoxide anion and ABTS free radicals,vitro antioxidant activity,and the inhibition of the activity of alpha glucosidase can be benefited from the total saponins in fruit of Rosa davuvrica Pall..The results provide a reference to further study on the total saponins in fruit of Rosa davuvrica Pall.
The purification effect of different models resin and their activity in vitro of the total saponins in fruit of Rosa davuvrica Pall.were studied.Eight types of macroporous resins,such as LSA-21,D-101 and AB-8,were used to purify the total saponins from Fruit of Rosa davuvrica Pall. Adsorption rate and desorption rate were determinated by ultraviolet-visible spectrophotometry method. The results showed that the LSA-21 type of macroporous resin had the best purification effect on the total saponins in fruit of Rosa davuvrica Pall.The best conditions were as follows: The concentration of adsorption liquid was 0.192 7 g/mL.The volume of adsorption liquid was 25 mL.The pH of adsorption liquid was 6.5.The volume fraction of ethanol was 90%.The volume of desorption liquid was 40 mL and the desorption solution pH was 8.5.With the above purification conditions,the calculated adsorption and desorption rate of the total saponins in fruit of Rosa davuvrica Pall. could reach77.51% and 65.28%,respectively.The content of the total saponins in dry extract was increased from 9.49% to33.65%.The test results showed that scavenging ability to DPPH·,·OH,superoxide anion and ABTS free radicals,vitro antioxidant activity,and the inhibition of the activity of alpha glucosidase can be benefited from the total saponins in fruit of Rosa davuvrica Pall..The results provide a reference to further study on the total saponins in fruit of Rosa davuvrica Pall.
引文
[1]郭海欢,王晓林,钟方丽,等.响应面法优化刺玫果黄酮苷元纤维素酶的酶解工艺研究[J].吉林化工学院学报,2017,34(3):10-15.
[2]陈帅,钟方丽,祝波.刺玫果药理学研究进展[J].吉林化工学院学报,2011,28(5):31-32.
[3]钟方丽,王晓林,张娜.刺玫果总皂苷的提取工艺研究[J].安徽农业科学,2011,39(3):1387-1389+1398.
[4]冯杉杉,金哲雄.刺玫果研究进展[J].黑龙江医药,2014,27(4):785-788.
[5]刘洋,刘海霞,马先红.天然植物中苷类化合物提取纯化方法的研究进展[J].吉林化工学报,2013,30(9):13-17.
[6]龚行楚,闫安忆,瞿海斌.大孔树脂分离纯化中草药中皂苷类成分的研究进展[J].世界科学技术-中医药现代化,2013,15(2):329-334.
[7]钟方丽,王晓林,付丽娟,等.大孔树脂法纯化刺玫果总皂苷工艺研究[J].河南工业大学学报:自然科学版,2014,35(1):76-80.
[8]汪忠波,敖明章.分光光度法测定竹节参中齐墩果酸皂甙含量[J].安徽农业科学,2007,35(26):8094-8095.
[9]赵昕,于一帆,苏婉莹,等.大孔树脂吸附分离黑胡萝卜红色素的研究[J].食品研究与开发,2017,38(23):38-43.
[10]赵贝塔,赵巍,刘邻渭,等.大孔树脂纯化酸石榴汁中花青素的研究[J].西北农林科技大学学报:自然科学版,2014,42(10):199-204.
[11]苏东晓,张瑞芬,张名位,等.荔枝果肉酚类物质大孔树脂分离纯化工艺优化[J].中国农业科学,2014,47(14):2897-2906.
[12]童鑫,张瑞芬,邓媛元,等.米糠酚类物质的大孔树脂分离纯化工艺[J].中国农业科学,2016,49(19):3818-3830.
[13]张慧君,王文霞,宋秘钊.大孔吸附树脂对金莲花黄色素的吸附性能研究[J].化学研究与应用,2009,21(4):472-476.
[14]张洪坤.大孔树脂吸附纯化茯苓多糖工艺研究[J].食品研究与开发,2017,38(23):67-71.
[15]李进,瞿伟菁,张素军,等.黑果枸杞色素的抗氧化活性研究[J].中国中药杂志,2006,31(14):1179-1183.
[16]张强,张黎明.阿魏酸茯苓多糖的抗氧化活性研究[J].现代食品科技,2011,27(9):1077-1089.
[17]符春丽,彭燕,黎诗敏,等.番石榴叶乙酸乙酯萃取物的体外抗氧化活性及化学成分的分离鉴定[J].现代食品科技,2017,33(10):52-57+20.
[18]崔同,李喜悦,王荣芳,等.山楂酒甲醇含量的测定及清除DPPH和ABTS自由基活性的研究[J].酿酒科技,2015(7):17-20.
[19]牛鹏飞,仇农学,杜寅.苹果渣中不同极性多酚的分离及体外抗氧化活性研究[J].农业工程学报,2008(3):238-242.
[20]赵文恩,李茜倩.FRAP法测定大枣枣皮红色素的总抗氧化能力[J].郑州大学学报:工学版,2011,32(3):28-30+35.
[21]李颖畅,孟宪军.蓝莓花色苷抗氧化活性的研究[J].食品与发酵工业,2007(9):61-64.
[22]王艳丽,许惠芳,郭瑜,等.大孔树脂纯化酸浆果实多酚及其生物活性研究[J].食品研究与开发,2018,39(2):13-20.
[2]陈帅,钟方丽,祝波.刺玫果药理学研究进展[J].吉林化工学院学报,2011,28(5):31-32.
[3]钟方丽,王晓林,张娜.刺玫果总皂苷的提取工艺研究[J].安徽农业科学,2011,39(3):1387-1389+1398.
[4]冯杉杉,金哲雄.刺玫果研究进展[J].黑龙江医药,2014,27(4):785-788.
[5]刘洋,刘海霞,马先红.天然植物中苷类化合物提取纯化方法的研究进展[J].吉林化工学报,2013,30(9):13-17.
[6]龚行楚,闫安忆,瞿海斌.大孔树脂分离纯化中草药中皂苷类成分的研究进展[J].世界科学技术-中医药现代化,2013,15(2):329-334.
[7]钟方丽,王晓林,付丽娟,等.大孔树脂法纯化刺玫果总皂苷工艺研究[J].河南工业大学学报:自然科学版,2014,35(1):76-80.
[8]汪忠波,敖明章.分光光度法测定竹节参中齐墩果酸皂甙含量[J].安徽农业科学,2007,35(26):8094-8095.
[9]赵昕,于一帆,苏婉莹,等.大孔树脂吸附分离黑胡萝卜红色素的研究[J].食品研究与开发,2017,38(23):38-43.
[10]赵贝塔,赵巍,刘邻渭,等.大孔树脂纯化酸石榴汁中花青素的研究[J].西北农林科技大学学报:自然科学版,2014,42(10):199-204.
[11]苏东晓,张瑞芬,张名位,等.荔枝果肉酚类物质大孔树脂分离纯化工艺优化[J].中国农业科学,2014,47(14):2897-2906.
[12]童鑫,张瑞芬,邓媛元,等.米糠酚类物质的大孔树脂分离纯化工艺[J].中国农业科学,2016,49(19):3818-3830.
[13]张慧君,王文霞,宋秘钊.大孔吸附树脂对金莲花黄色素的吸附性能研究[J].化学研究与应用,2009,21(4):472-476.
[14]张洪坤.大孔树脂吸附纯化茯苓多糖工艺研究[J].食品研究与开发,2017,38(23):67-71.
[15]李进,瞿伟菁,张素军,等.黑果枸杞色素的抗氧化活性研究[J].中国中药杂志,2006,31(14):1179-1183.
[16]张强,张黎明.阿魏酸茯苓多糖的抗氧化活性研究[J].现代食品科技,2011,27(9):1077-1089.
[17]符春丽,彭燕,黎诗敏,等.番石榴叶乙酸乙酯萃取物的体外抗氧化活性及化学成分的分离鉴定[J].现代食品科技,2017,33(10):52-57+20.
[18]崔同,李喜悦,王荣芳,等.山楂酒甲醇含量的测定及清除DPPH和ABTS自由基活性的研究[J].酿酒科技,2015(7):17-20.
[19]牛鹏飞,仇农学,杜寅.苹果渣中不同极性多酚的分离及体外抗氧化活性研究[J].农业工程学报,2008(3):238-242.
[20]赵文恩,李茜倩.FRAP法测定大枣枣皮红色素的总抗氧化能力[J].郑州大学学报:工学版,2011,32(3):28-30+35.
[21]李颖畅,孟宪军.蓝莓花色苷抗氧化活性的研究[J].食品与发酵工业,2007(9):61-64.
[22]王艳丽,许惠芳,郭瑜,等.大孔树脂纯化酸浆果实多酚及其生物活性研究[J].食品研究与开发,2018,39(2):13-20.