小儿血管瘤各期CXCL12/CXCR4、VEGF的表达及其临床意义
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摘要
目的分析各期小儿血管瘤CXCL12/CXCR4及VEGF的表达变化规律,以期为临床诊断及治疗提供参考。方法收集经病理学诊断为小儿血管瘤的患者临床资料共210例,其中增生期血管瘤患者90例,消退期血管瘤患者120例。以海南医学院第一附属医院儿科同期健康体检者60例为对照组。采用定量PCR及酶联免疫吸附测定法分析各组血清中CXCL12/CXCR4及VEGF的表达。结果小儿血管瘤组CXCL12/CXCR4和VEGF及其受体m RNA及蛋白水平明显高于对照组(P<0.05),且增生期血管瘤患儿水平显著高于消退期血管瘤患儿(P<0.05)。结论 CXCL12/CXCR4及VEGF在小儿血管瘤增生期及消退期表达有明显差异,提示其参与了小儿血管瘤病理演变。
Objective The present research tried to explore the role of CXCL12/CXCR4 and VEGF in the development of children hemangioma. Methods 210 cases of children hemangioma patients in our department were enrolled in this part. The expression of CXCL12/CXCR4 and VEGF were assayed in patients with different stages of children hemangioma. 60 healthy subjects were taken as control group at the same period. Results The results showed that the m RNA and protein levels of CXCL12/CXCR4 and VEGF were higher in children hemangioma patients than in control group(P<0.05). At the same time, the m RNA and protein levels of CXCL12/CXCR4 and VEGF were higher in proliferating stage than in involuting stage patients, with a statistical difference(P<0.05). Conclusion CXCL12/CXCR4 and VEGF levels have a close relationship with the pathological process of children hemangioma.
Objective The present research tried to explore the role of CXCL12/CXCR4 and VEGF in the development of children hemangioma. Methods 210 cases of children hemangioma patients in our department were enrolled in this part. The expression of CXCL12/CXCR4 and VEGF were assayed in patients with different stages of children hemangioma. 60 healthy subjects were taken as control group at the same period. Results The results showed that the m RNA and protein levels of CXCL12/CXCR4 and VEGF were higher in children hemangioma patients than in control group(P<0.05). At the same time, the m RNA and protein levels of CXCL12/CXCR4 and VEGF were higher in proliferating stage than in involuting stage patients, with a statistical difference(P<0.05). Conclusion CXCL12/CXCR4 and VEGF levels have a close relationship with the pathological process of children hemangioma.
引文
[1]Hoornweg MJ,Theunissen CI,Hage JJ,et al.Malignant differential diagnosis in children referred for infantile hemangioma[J].Ann Plast Surg.2015,74(1):43-46.DOI:10.1097/SAP.0b013e31828bb2d9.
[2]Tanyildiz HG,Unal EC,Gokce H,et al.Serum basic fibroblastic growth factor levels in children with infantile hemangioma[J].Indian J Pediatr.2016,83(9):937-940.DOI:10.1007/s12098-016-2041-2.
[3]Rubie C,Kauffels A,Kolsch K,et al.Cxcl12/cxcr4 display an inverse mrna expression profile in gastric carcinoma that correlates with tumor progression[J].Oncol Lett.2016,11(1):360-364.DOI:10.3892/ol.2015.3850.
[4]Wyse MM,Goicoechea S,Garcia-Mata R,et al.Mdia2 and cxcl12/cxcr4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions[J].Biochem Biophys Res Commun.2017,484(2):255-261.DOI:10.1016/j.bbrc.2017.01.087.
[5]Yu X,Li W,Deng Q,et al.Neoalbaconol inhibits angiogenesis and tumor growth by suppressing egfr-mediated vegf production[J].Mol Carcinog.2017,56(5):1414-1426.DOI:10.1002/mc.22602.
[6]Conley-La Comb MK,Saliganan A,Kandagatla P,et al.Pten loss mediated akt activation promotes prostate tumor growth and metastasis via cxcl12/cxcr4 signaling[J].Mol Cancer.2013,12(1):85.DOI:10.1186/1476-4598-12-85.
[7]Gil M,Seshadri M,Komorowski MP,et al.Targeting cxcl12/cxcr4 signaling with oncolytic virotherapy disrupts tumor vasculature and inhibits breast cancer metastases[J].Proc Natl Acad Sci U S A.2013,110(14):E1291-1300.DOI:10.1073/pnas.1220580110.
[8]Holland JD,Gyorffy B,Vogel R,et al.Combined wnt/betacatenin,met,and cxcl12/cxcr4 signals characterize basal breast cancer and predict disease outcome[J].Cell Rep.2013,5(5):1214-1227.DOI:10.1016/j.celrep.2013.11.001.
[9]Wu W,Qian LY,Dai J,et al.Expression and clinical significance of chemokine CXCL12 with its receptor CXCR4 and CXCR7 in human breast cancer tissue[J].Chin J Gene Surg,2015,24(11):1577-1582.DOI:10.13547/j.issn.1000-1719.2015.011.044.
[10]Qiao CX,Zhang XF,Cheng XF,et al.Effects of dry toad skin extracts on CXCL12/CXCR4 axis in nude mice with liver metastasis of gastric cancer[J].Laoning J Trad Chin Med,2017,44(8):1736-1738.DOI:10.13192/j.issn.1000-1719.2017.08.062.
[11]Yin D,Zhang Z,Gao S,et al.The role of chemokine receptor and its receptor CXCR4 and its ligand CXCR12 in the process of proliferation and migration of oral squamous cell carcinoma[J].West Chin J Stomatol,2013,31(1):8-12.DOI:10.16578/j.issn.1040-1719.2013.001.043.
[12]Yu SX,Li J.The relationship between CXCL12/CXCR4-CXCR7 axis signal transduction and the biological features of tumor[J].Basic Clin Med,2012,32(3):349-353.DOI:10.16352/j.issn.1001-6325.2012.03.028.
[13]Lawicki S,Zajkowska M,Glazewska EK,et al.Plasma levels and diagnostic utility of vegf,mmp-2 and timp-2 in the diagnostics of breast cancer patients[J].Biomarkers.2017;22(2):157-164.DOI:10.1080/1354750X.2016.1252955.
[14]Liu Y,Li F,Gao F,et al.Periostin promotes tumor angiogenesis in pancreatic cancer via erk/vegf signaling[J].Oncotarget.2016;7(26):40148-40159.DOI:10.18632/oncotarget.9512.
[15]Feng YL,Qu C,Wang P,et al.Expression of chemokine receptors,MMPs and VEGF in pancreatic cancer fibroblast cell lines[J].Chin J Oncol Prev and Treat,2016,8(4):212-216.DOI:10.1547/j.issn.1090-1719.2016.0041.087.
[16]Jiang H,Mao XG.Effects on expression of blood and tumor tissue MMP2,MMP9,VEGF in mouse cervical carcinoma xenograft model[J].J Clin Experiment Med,2016,15(9):832-835.DOI:10.1050/j.issn.1000-1548.2016.015.089.
[2]Tanyildiz HG,Unal EC,Gokce H,et al.Serum basic fibroblastic growth factor levels in children with infantile hemangioma[J].Indian J Pediatr.2016,83(9):937-940.DOI:10.1007/s12098-016-2041-2.
[3]Rubie C,Kauffels A,Kolsch K,et al.Cxcl12/cxcr4 display an inverse mrna expression profile in gastric carcinoma that correlates with tumor progression[J].Oncol Lett.2016,11(1):360-364.DOI:10.3892/ol.2015.3850.
[4]Wyse MM,Goicoechea S,Garcia-Mata R,et al.Mdia2 and cxcl12/cxcr4 chemokine signaling intersect to drive tumor cell amoeboid morphological transitions[J].Biochem Biophys Res Commun.2017,484(2):255-261.DOI:10.1016/j.bbrc.2017.01.087.
[5]Yu X,Li W,Deng Q,et al.Neoalbaconol inhibits angiogenesis and tumor growth by suppressing egfr-mediated vegf production[J].Mol Carcinog.2017,56(5):1414-1426.DOI:10.1002/mc.22602.
[6]Conley-La Comb MK,Saliganan A,Kandagatla P,et al.Pten loss mediated akt activation promotes prostate tumor growth and metastasis via cxcl12/cxcr4 signaling[J].Mol Cancer.2013,12(1):85.DOI:10.1186/1476-4598-12-85.
[7]Gil M,Seshadri M,Komorowski MP,et al.Targeting cxcl12/cxcr4 signaling with oncolytic virotherapy disrupts tumor vasculature and inhibits breast cancer metastases[J].Proc Natl Acad Sci U S A.2013,110(14):E1291-1300.DOI:10.1073/pnas.1220580110.
[8]Holland JD,Gyorffy B,Vogel R,et al.Combined wnt/betacatenin,met,and cxcl12/cxcr4 signals characterize basal breast cancer and predict disease outcome[J].Cell Rep.2013,5(5):1214-1227.DOI:10.1016/j.celrep.2013.11.001.
[9]Wu W,Qian LY,Dai J,et al.Expression and clinical significance of chemokine CXCL12 with its receptor CXCR4 and CXCR7 in human breast cancer tissue[J].Chin J Gene Surg,2015,24(11):1577-1582.DOI:10.13547/j.issn.1000-1719.2015.011.044.
[10]Qiao CX,Zhang XF,Cheng XF,et al.Effects of dry toad skin extracts on CXCL12/CXCR4 axis in nude mice with liver metastasis of gastric cancer[J].Laoning J Trad Chin Med,2017,44(8):1736-1738.DOI:10.13192/j.issn.1000-1719.2017.08.062.
[11]Yin D,Zhang Z,Gao S,et al.The role of chemokine receptor and its receptor CXCR4 and its ligand CXCR12 in the process of proliferation and migration of oral squamous cell carcinoma[J].West Chin J Stomatol,2013,31(1):8-12.DOI:10.16578/j.issn.1040-1719.2013.001.043.
[12]Yu SX,Li J.The relationship between CXCL12/CXCR4-CXCR7 axis signal transduction and the biological features of tumor[J].Basic Clin Med,2012,32(3):349-353.DOI:10.16352/j.issn.1001-6325.2012.03.028.
[13]Lawicki S,Zajkowska M,Glazewska EK,et al.Plasma levels and diagnostic utility of vegf,mmp-2 and timp-2 in the diagnostics of breast cancer patients[J].Biomarkers.2017;22(2):157-164.DOI:10.1080/1354750X.2016.1252955.
[14]Liu Y,Li F,Gao F,et al.Periostin promotes tumor angiogenesis in pancreatic cancer via erk/vegf signaling[J].Oncotarget.2016;7(26):40148-40159.DOI:10.18632/oncotarget.9512.
[15]Feng YL,Qu C,Wang P,et al.Expression of chemokine receptors,MMPs and VEGF in pancreatic cancer fibroblast cell lines[J].Chin J Oncol Prev and Treat,2016,8(4):212-216.DOI:10.1547/j.issn.1090-1719.2016.0041.087.
[16]Jiang H,Mao XG.Effects on expression of blood and tumor tissue MMP2,MMP9,VEGF in mouse cervical carcinoma xenograft model[J].J Clin Experiment Med,2016,15(9):832-835.DOI:10.1050/j.issn.1000-1548.2016.015.089.