植物嫁接砧穗愈合机制研究进展
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摘要
植物嫁接砧穗愈合是指同种或异种植物的细胞、组织或器官互相影响与作用,结合成一个完整有机体的过程,受砧穗自身和外界等多种因素的调节和控制,是植物学领域的研究热点。嫁接可使植物的产量及品质得到提升,并有助于植物更好地适应环境。了解植物嫁接砧穗愈合机制对提高嫁接成活率和嫁接技术在生产中的应用具有重要的意义。就当前植物嫁接砧穗愈合的形态学进程、生理生化机制、分子调控及影响因素等方面的研究进展进行了综述,并对未来研究方向进行了展望。参71
Plant grafting healing is a process in which the same or heterogeneous plant's cells, tissues or organs interact and interplay to form a complete organism. The process is not only regulated by the scion and rootstock, but also influenced by some external factors. It has become a hotspot research area in botany. Grafting can improve plant productivity and quality and help the plant adapt to environment. Understanding the mechanism of graft healing makes a great significance to grafting survival rate and the application of grafting technology in production. This paper reviewed the morphological process, physiological and biochemical mechanism of grafted healing as well as its molecular regulation and determinants, and proposed several directions for future research. [Ch, 71 ref.]
Plant grafting healing is a process in which the same or heterogeneous plant's cells, tissues or organs interact and interplay to form a complete organism. The process is not only regulated by the scion and rootstock, but also influenced by some external factors. It has become a hotspot research area in botany. Grafting can improve plant productivity and quality and help the plant adapt to environment. Understanding the mechanism of graft healing makes a great significance to grafting survival rate and the application of grafting technology in production. This paper reviewed the morphological process, physiological and biochemical mechanism of grafted healing as well as its molecular regulation and determinants, and proposed several directions for future research. [Ch, 71 ref.]
引文
[1]LI Shengyou,TENG Fei,RAO Demin,et al.Agronomic traits of soybean cultivars released in different decades after grafting record-yield cultivar as rootstock[J].Plant Breed,2017,136:133-138.doi:10.1111/pbr.12436.
[2]LOUWS F J,RIVARD C L,KUBOTA C.Grafting fruiting vegetables to manage soilborne pathogens,foliar pathogens,arthropods and weeds[J].Sci Hortic,2010,127(2):127-146.
[3]LEE J M,ODA M.Grafting of herbaceous vegetable and ornamental crops[J].Hortic Rev,2010,28:61-124.
[4]LIU Binghua,LI Mingjun,CHENG Liang,et al.Influence of rootstock on antioxidant system in leaves and roots of young apple trees in response to drought stress[J].Plant Growth Regul,2012,67(3):247-256.
[5]DAVIS A R,PERKINS-VEAZIE P,SAKATA Y,et al.Cucurbit grafting[J].Crit Rev Plant Sci,2008,27(1):50-74.
[6]ROUPHAEL Y,SCHWARZ D,KRUMBEIN A,et al.Impact of grafting on product quality of fruit vegetables[J].Sci Hortic,2010,127(2):172-179.
[7]YAMATO T,DAN K.Improved growth and yield under high-temperature conditions of Cucurbita maxima Duchesne ex Lam.cv.Kurokawa-delicious grafted onto Cucurbita moschata Duchesne ex Poir.cv.Shima-kabocha as rootstock[J].Hortic Res,2015,14(2):171-177.
[8]YOONAH J,JIHYE M,JIWEON L,et al.Effects of different rootstocks on fruit quality of grafted pepper(Capsicum annuum L.)[J].Korean J Hortic Sci Technol,2013,31(6):687-699.
[9]KUBOTA C,Mc CLURE M A,KOKALIS-BURELLE N,et al.Vegetable grafting:history,use,and current technology status in North America[J].Hort Science,2008,43(6):1664-1669.
[10]胡艳青,苏媛,韩风叶,等.嫁接黄瓜在愈合过程中的解剖观察和抗氧化酶活性的变化研究[J].内蒙古农业大学学报,2007,28(3):224-230.HU Yanqing,SU Yuan,HAN Fengye,et al.The research of anatomical structures and three activity of antioxidases change of grafed cucumber seedling[J].J Inn Mong Agric Univ,2007,28(3):224-230.
[11]杨欢欢,李景富.不同砧木黄瓜/南瓜嫁接愈合过程的解剖学观察[J].北方园艺,2014(8):5-8.YANG Huanhuan,LI Jingfu.The anatomical observation of different root stock of healing process on the grafted cucumber/pumpkin[J].Northern Hortic,2014(8):5-8.
[12]黄坚钦,章滨森,陆建伟,等.山核桃嫁接愈合过程的解剖学观察[J].浙江林学院学报,2001,18(2):111-114.HUANG Jianqin,ZHANG Binsen,LU Jianwei,et al.Anatomical observation in graft union of Carya cathayenisis[J].J Zhejiang For Coll,2001,18(2):111-114.
[13]张红梅,丁明,姜武,等.不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究[J].园艺学报,2012,39(3):493-500.ZHANG Hongmei,DING Ming,JIANG Wu,et al.Histological and cytological research on graft union formation of grafted watermelon seedlings with different scion ages[J].Acta Hortic Sin,2012,39(3):493-500.
[14]朱晓慧,杨途熙,魏安智,等.无刺花椒嫁接愈合过程中相关生理指标的变化[J].西北林学院学报,2015,30(2):134-138.ZHU Xiaohui,YANG Tuxi,WEI Anzhi,et al.Changes of relative physiological indices involved in the graft union development of Zanthoxylum bungeanum[J].J Northwest For Univ,2015,30(2):134-138.
[15]蒋丽娟,马倩,佟金权,等.光皮树砧木生长指标及内源激素对嫁接成活率的影响[J].中南林业科技大学学报,2011,31(3):26-29.JIANG Lijuan,MA Qian,TONG Jinquan,et al.Effects of growth indices and endogenous hormone on grafting survival rate of Cornus wilsoniana[J].J Cent South Univ For Technol,2011,31(3):26-29.
[16]李锋.植物嫁接不亲和性的问题讨论[J].惠州学院学报(自然科学版),1991,17(4):170-172.LI Feng.Discussion on grafting incompatibility of plant[J].J Huizhou Univ Nat Sci,1991,17(4):170-172.
[17]CHINALIA F A,REGALI-SELEGHIN M H,CORREA E M.2,4-D toxicity:cause,effect and control[J].Terr Aquat Environ Toxicol,2007,1(2):24-33.
[18]高京草,王慧霞,李西选.可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究[J].北方园艺,2010(23):18-20.GAO Jingcao,WANG Huixia,LI Xixuan.Relationship between soluble protein,MDA and jujube tree cold hardiness[J].Northern Hortic,2010(23):18-20.
[19]商闯,马春红,翟彩霞,等.丙二醛(MDA)含量在玉米诱导抗病过程中的变化[J].华北农学报,2007,22(增刊):29-32.SHANG Chuang,MA Chunhong,ZHAI Caixia,et al.The changes of malondialdenyde content on course of the induced disease resistance[J].Acta Agric Boreali-Sin,2007,22(suppl):29-32.
[20]丁锦平,徐心诚,李文琦.番茄嫁接后生理指标的变化[J].北方园艺,2015(5):29-31.DING Jinping,XU Xincheng,LI Wenqi.Physiological changes of tomato after grafting[J].Northern Hortic,2015(5):29-31
[21]陈红,王永清,袁媛,等.茄子/番茄嫁接体发育过程中的蛋白质含量、POD、CAT和SOD活性及其同工酶研究[J].四川农业大学学报,2006,24(2):144-147.CHEN Hong,WANG Yongqing,YUAN Yuan,et al.Studies on protein content,activity and enzymes of POD,SOD and CAT in eggplant/tomato graft union[J].J Sichuan Agric Univ,2006,24(2):144-147.
[22]王超霞,邓嘉进,张雅丽,等.不同葡萄砧木对矢富罗莎果肉酚类化合物含量的影响[J].中外葡萄与葡萄酒,2011(7):28-31.WANG Chaoxia,DENG Jiajin,ZHANG Yali,et al.Influence of different grapevine rootstocks on the phenols content of Yatomi Rosa[J].Sino-Oversea Grape Wine,2011(7):28-31.
[23]张力平,孙长霞,李俊清,等.植物多酚的研究现状及发展前景[J].林业科学,2005,41(6):157-162.ZHANG Liping,SUN Changxia,LI Junqing,et al.The present conditions and development trend of plant polyphenols research[J].Sci Silv Sin,2005,41(6):157-162.
[24]郭大勇,谭琪,夏仁学.日本栗/板栗酚类物质含量与嫁接亲和力关系研究[J].华中农业大学学报,2004,23(3):341-343.GUO Dayong,TAN Qi,XIA Renxue.Studies on relationship between phenol content and graft compatibility in chestnut[J].J Huazhong Agric Univ,2004,23(3):341-343.
[25]马绍英,刘元卫,苏李维,等.葡萄离体嫁接部位的生理生化变化[J].果树学报,2016,33(2):179-186.MA Shaoying,LIU Yuanwei,SU Liwei,et al.Physiological and biochemical changes in the graft site after in vitro grafting in grape[J].J Fruit Sci,2016,33(2):179-186.
[26]雷东锋,蒋大宗,王一理.烟草中多酚氧化酶的生理生化特征及其活性控制的研究[J].西安交通大学学报,2003,37(12):1316-1320.LEI Dongfeng,JIANG Dazong,WANG Yili.Physiological and biochemical characteristic of tobacco polyphenol oxidase and control way for its activity[J].J Xi’an Jiaotong Univ,2003,37(12):1316-1320.
[27]陈静,符真珠,王政,等.牡丹多酚氧化酶的酶学特性[J].西北农林科技大学学报(自然科学版),2012,40(3):154-157.CHEN Jing,FU Zhenzhu,WANG Zheng,et al.Study on characteristics of polyphenol oxidase in peony[J].J Northwest A&F Univ Nat Sci Ed,2012,40(3):154-157.
[28]张淑红,王月,聂洪光,等.茄子嫁接愈合过程中相关酶活性的变化[J].沈阳农业大学学报,2016,47(3):266-270.ZHANG Shuhong,WANG Yue,NIE Hongguang,et al.Changes of related enzyme activity during healing process of grafted eggplant[J].J Shenyang Agric Univ,2016,47(3):266-270.
[29]严毅,高柱,何承忠,等.葡萄柚嫁接愈合过程关联酶活性研究进展[J].安徽农业科学,2011,39(2):734-736,742.YAN Yi,GAO Zhu,HE Chengzhong,et al.Research progress of related enzyme activity during graft healing process of grapefruit[J].J Anhui Agric Sci,2011,39(2):734-736,742.
[30]孙华丽,宋健坤,李鼎立,等.梨不同嫁接组合嫁接愈合过程中生理动态变化研究[J].北方园艺,2013(16):25-29.SUN Huali,SONG Jiankun,LI Dingli,et al.Study on the physiological dynamic change of different graft combinations in pear during the grafted healing process[J].Northern Horti,2013(16):25-29.
[31]曲云峰,赵忠,张小鹏.大扁杏嫁接愈合过程中几种生化物质含量的变化[J].西北农林科技大学学报(自然科学版),2008,36(5):73-78.QU Yunfeng,ZHAO Zhong,ZHANG Xiaopeng.The content changes of several biochemistry substances of grafted armeniaca surviving[J].J Northwest A&F Univ Nat Sci Ed,2008,36(5):73-78.
[32]张红梅,黄丹枫,丁明,等.不同苗龄接穗的西瓜嫁接体愈合过程中的3种酶活性变化[J].植物生理学通讯,2005,41(3):302-304.ZHANG Hongmei,HUANG Danfeng,DING Ming,et al.Changes in three enzyme activities in the prcess of watermelon seedlings grafted with different ages of scion[J].Plant Physiol Commun,2005,41(3):302-304.
[33]王瑞,陈永忠,王湘南,等.油茶芽苗砧嫁接愈合过程中砧穗相关生理指标的研究[J].西北农林科技大学学报(自然科学版),2014,42(1):46-50.WANG Rui,CHEN Yongzhong,WANG Xiangnan,et al.Dynamic changes in physiological indexes of Camellia oleifera nurse seed graft in the grafting and recovering process[J].J Northwest A&F Univ Nat Sci Ed,2014,42(1):46-50.
[34]程勋东,李姗姗,隋益虎,等.辣椒/茄子嫁接体保护酶同工酶分析[J].安徽科技学院学报,2015,29(3):14-17.CHENG Xundong,LI Shanshan,SUI Yihu,et al.Analysis of protecting isoenzymes of pepper/eggplant graft union[J].J Anhui Sci Technol Univ,2015,29(3):14-17.
[35]张子学,李爱青,许玉龙,等.西瓜嫁接苗的植物保护酶同工酶与亲和力分析[J].热带作物学报,2006,27(1):12-16.ZHANG Zixue,LI Aiqing,XU Yulong,et al.Analysis of plant protecting isozymes and theirs affinity of grafted watermelon seedlings[J].Chin J Trop Crops,2006,27(1):12-16.
[36]邰翔,郭世荣,陈志贵,等.以耐盐茄子为砧木的番茄嫁接苗的分析[J].江苏农业学报,2010,26(3):583-586.TAI Xiang,GUO Shirong,CHEN Zhigui,et al.Analysis of tomato seedlings grafted with salt tolerance eggplant rootstock[J].Jiangsu J Agric Sci,2010,26(3):583-586.
[37]冯金玲,杨志坚,陈辉.油茶芽苗砧嫁接体愈合过程AFLP分析[J].中南林业科技大学学报,2012,32(3):141-146.FENG Jinling,YANG Zhijian,CHEN Hui.AFLP analysis of nurse seed grafted union in Camellia oleifera during healing[J].J Cent South Univ For Technol,2012,32(3):141-146.
[38]YIN Hao,YAN Bo,SUN Jing,et al.Graft-union development:a delicate process that involves cell-cell communication between scion and stock for local auxin accumulation[J].J Exp Bot,2012,63(11):4219-4232.
[39]QIU Lingling,JIANG Bo,FANG Jia,et al.Analysis of transcriptome in hickory(Carya cathayensis),and uncover the dynamics in the hormonal signaling pathway during graft process[J].BMC Genom,2016,17(1):935.doi:10.1186/s12864-016-3182-4.
[40]COOKSON S J,MORENO M J C,HEVIN C,et al.Graft union formation in grapevine induces tran scriptional changes related to cell wall modification,wounding,hormone signalling,and secondary metabolism[J].J Exp Bot,2013,64(10):2997-3008.
[41]田晓彬,齐红岩,李岩,等.不同砧木嫁接对薄皮甜瓜成熟期品质、香气合成相关酶活性及基因表达的影响[J].中国农业科学,2012,45(9):1766-1774.TIAN Xiaobin,QI Hongyan,LI Yan,et al.Effects of different rootstocks on ripening quality,activities and gene expression of aroma-related enzymes in grafting oriental melon(Cucumis melo var.makuwa Makino)[J].Sci Agric Sin,2012,45(9):1766-1774.
[42]AYRE B G,TURGEON R.Graft transmission of a floral stimulant derived from constans[J].Plant Physiol,2004,135(4):2271-2278.
[43]LIU Na,YANG Jinghua,GUO Shaogui,et al.Genome-Wide identification and comparative analysis of conserved and novel micro RNAs in grafted watermelon by high-throughput sequencing[J].PLo S One,2013,8(2):e57359.doi:10.1371/journal.pone.0057359
[44]YEOMAN M M,KILPATRICK D C,MIEDZYBRODZKA M B,et al.Cellular interactions during graft formation in plants,a recognition phenomenon?[J].Symp Soc Exp Biol,1978,32(3):139-160.
[45]曾义安,朱月林,黄保健,等.嫁接黄瓜的光合特性及叶片激素含量和可溶性蛋白研究[J].南京农业大学学报,2005,28(1):16-19.ZENG Yian,ZHU Yuelin,HUANG Baojian,et al.Study on photosynthetic characteristics,GA and ABA contents,and soluble proteins in leaves of grafted cucumber[J].J Nanjing Agric Univ,2005,28(1):16-19.
[46]李跃建,梁根云,刘小俊,等.黄瓜嫁接苗和自根苗的蛋白质组学研究[J].园艺学报,2009,36(8):1147-1152.LI Yuejian,LIANG Genyun,LIU Xiaojun,et al.Proteomic study on grafted and non-grafted cucumber(Cucumis sativus L.)[J].Acta Hortic Sin,2009,36(8):1147-1152.
[47]XU Qing,GUO Shirong,LI Lin,et al.Proteomics analysis of compatibility and incompatibility in grafted cucumber seedlings[J].Plant Physiol Biochem,2016,105(4):21-28.doi:10.1016/j.plaphy.2016.04.001
[48]冯金玲,杨志坚,陈辉.油茶芽苗砧嫁接口不同发育时期差异蛋白质分析[J].应用生态学报,2012,23(8):2055-2061.FENG Jinling,YANG Zhijian,CHEN Hui.Analysis of differential proteins in nurse seed grafted unions of Camellia oleifera at its different developmental stages[J].Chin J Appl Ecol,2012,23(8):2055-2061.
[49]ODA M,MARUYAMA M,MORI G.Water transfer at graft union of tomato plants grafted onto solanum rootstocks[J].Engei Gakkai Zasshi,2015,74(6):458-463.
[50]刘传荷,洪健,夏国华,等.山核桃嫁接过程中愈伤反应的细胞学观察[J].林业科学,2009,45(6):34-38.LIU Chuanhe,HONG Jian,XIA Guohua,et al.Cytological observation on healing responses in grafting of Carya cathayensis[J].Sci Silv Sin,2009,45(6):34-38.
[51]GISBERT C,PROHENS J,RAIG魷N M D,et al.Eggplant relatives as sources of variation for developing new rootstocks:effects of grafting on eggplant yield and fruit apparent quality and composition[J].Sci Hortic,2001,128(1):14-22.
[52]张帆,贾长才,姜立纲,等.黄瓜专用砧木品种京欣砧5号的选育[J].中国蔬菜,2011(4):73-75.ZHANG Fan,JIA Changcai,JIANG Ligang,et al.A new cucumber rootstock F1 hybrid-‘Jingxinzhen No.5’[J].China Veget,2011(4):73-75.
[53]施先锋,李爱成,李煜华,等.西瓜专用砧木:鄂砧1号葫芦的选育[J].长江蔬菜,2012(6):17-19.SHI Xianfeng,LI Aicheng,LI Yuhua,et al.Breeding of a new watermelon rootstock cultivar,E-zhen No.1[J].J Changjiang Veget,2012(6):17-19.
[54]潘云平,张西森,王迎春,等.不同砧木嫁接西红柿亲和性试验研究[J].现代农业科技,2012(10):109-110.PAN Yunping,ZHANG Xisen,WANG Yingchun,et al.Study on compatibility of grafted tomatoes with different rootstocks[J].Mod Agric Technol,2012(10):109-110.
[55]VU N T,XU Zhihao,KIM Y S,et al.Effect of nursery environmental condition and different cultivars on survival rate of grafted tomato seedling[J].Acta Hortic,2014,1037(2):765-770.
[56]KAWAGUCHI M,TAJI A,BACKHOUSE D,et al.Anatomy and physiology of graft incompatibility in solanaceous plants[J].J Hortic Sci Biotechnol,2008,83(5):581-588.
[57]王白坡,程晓建,喻卫武.山核桃嫁接育苗成活率探讨[J].浙江林学院学报,2002,19(3):231-234.WANG Baipo,CHENG Xiaojian,YU Weiwu.Inquiry into survival rate of Carya cathayensis seedlings in grafting[J].J Zhejiang For Coll,2002,19(3):231-234.
[58]胡靖楠,蒋细春,林跃,等.化香作砧木嫁接山核桃育苗及造林效果[J].湖南林业科技,2009,36(5):11-14.HU Jingnan,JIANG Xichun,LIN Yue,et al.Effect of grafting Carya cathayenisis onto Platycarya strobilaceab on seedling-raising and planting[J].J Hunan For Sci Technol,2009,36(5):11-14.
[59]MUNEER S,KO C H,SOUNDARARAJAN P,et al.Proteomic study related to vascular connections in watermelon scions grafted onto bottle-gourd rootstock under different light intensities[J].PLo S One,2015,1 0(3):e0120899.doi:10.1371/journal.pone.0120899.
[60]宮本眞吾.LED照明を用いた物工場[J].表面技術,2010,61(9):641-645.MIYAMOTO S.Advances in plant factories with LED lighting[J].J Surf Finish Soc Jpn,2010,61(9):641-645.
[61]LEE K M,LIM C S,MUNEER S,et al.Functional vascular connections and light quality effects on tomato grafted unions[J].Sci Hortic,2016,201:306-317.doi:org/10.1016/j.scienta.2016.02013.
[62]赵锋亮.长期夜间亚低温和长期昼间亚高温对茄子生长发育的影响[D].郑州:河南农业大学,2008.ZHAO Fengliang.Effects of Long Team Night Sub-low Temperature and Long Team Day Sub-high Temperature on Eggplant Growth and Development[D].Zhengzhou:Henan Agricultural University,2008.
[63]王利民,王献革,李军英,等.观赏植物火鹤、红宝石喜林芋的快速繁殖[J].华北农学报,2002,17(增刊):213-218.WANG Limin,WANG Xiange,LI Junying,et al.Tissue cluture and rapid propagation of Anthurium scherzrianum and Philodendron enbesoens cv Red Emerald of modern ornamental aroid[J].Acta Agric Boreal-Sin,2002,17(supp1):213-218.
[64]李梅,须晖,李天来,等.不同夜温对番茄叶片呼吸及光合启动时间的影响[J].石河子大学学报(自然科学版),2006,24(2):201-204.LI Mei,XU Hui,LI Tianlai,et al.Influence of different night temperature the starting time of photosynthesis to tomato leaves[J].J Shihezi Univ Nat Sci,2006,24(2):201-204.
[65]赵渊渊,董春娟,尚庆茂.夜温对番茄套管嫁接苗愈合的影响[J].西北植物学报,2015,35(3):493-499.ZHAO Yuanyuan,DONG Chunjuan,SHANG Qingmao,et al.Healing responses of tube grafted tomato plug seedling under different night temperatures[J].Acta Bot Boreal-Occident Sin,2015,35(3):493-499.
[66]于金超,辜松,陈克农.利用小拱棚愈合嫁接苗的试验研究[J].农机化研究,2008(1):154-155.YU Jinchao,GU Song,CHEN Kenong.A study on the small arch awning of grafting seedling for healing[J].J Agric Mechan Res,2008(1):154-155.
[67]SHIBUYA T,ITAGAKI K,WANG Yu,et al.Grafting transiently suppresses development of powdery mildew colonies,probably through a quantitative change in water relations of the host cucumber scions during graft healing[J].Sci Hortic,2015,192:197-199.doi:org/10.1016/j.scienta.2015.06.010.
[68]MUN B,JANG Y,GOTO E,et al.Measurement system of whole-canopy carbon dioxide exchange rates in grafted cucumber transplants in which scions were exposed to different water regimes using a semi-open multi-chamber[J].Sci Hortic,2011,130(3):607-614.doi:org/10.1016/j.scienta.2011.08.017.
[69]郑碧娟,苏丽芳,陈世品,等.外源激动素对油茶芽苗砧嵌合体愈合的效应[J].福建农林大学学报(自然科学版),2014,43(2):146-150.ZHENG Bijuan,SU Lifang,CHEN Shipin,et al.Effects of exogenous KT on the healing of the grafted union of Camelia oleifera nurse seed[J].J Fujian Agric For Univ Nat Sci Ed,2014,43(2):146-150.
[70]赵琳,黄钰,李甜江,等.云油茶5个品种芽苗砧嫁接技术研究[J].西部林业科学,2012,41(5):19-25.ZHAO Lin,HUANG Yu,LI Tianjiang,et al.Study on seedling stock grafting of five varieties of‘Yunyou’series of Camellia oleifera[J].J West China For Sci,2012,41(5):19-25.
[71]GROSSMANN K.Auxin herbicide action:lifting the veil step by step[J].Plant Sign Behav,2007,2(5):421-423.
[2]LOUWS F J,RIVARD C L,KUBOTA C.Grafting fruiting vegetables to manage soilborne pathogens,foliar pathogens,arthropods and weeds[J].Sci Hortic,2010,127(2):127-146.
[3]LEE J M,ODA M.Grafting of herbaceous vegetable and ornamental crops[J].Hortic Rev,2010,28:61-124.
[4]LIU Binghua,LI Mingjun,CHENG Liang,et al.Influence of rootstock on antioxidant system in leaves and roots of young apple trees in response to drought stress[J].Plant Growth Regul,2012,67(3):247-256.
[5]DAVIS A R,PERKINS-VEAZIE P,SAKATA Y,et al.Cucurbit grafting[J].Crit Rev Plant Sci,2008,27(1):50-74.
[6]ROUPHAEL Y,SCHWARZ D,KRUMBEIN A,et al.Impact of grafting on product quality of fruit vegetables[J].Sci Hortic,2010,127(2):172-179.
[7]YAMATO T,DAN K.Improved growth and yield under high-temperature conditions of Cucurbita maxima Duchesne ex Lam.cv.Kurokawa-delicious grafted onto Cucurbita moschata Duchesne ex Poir.cv.Shima-kabocha as rootstock[J].Hortic Res,2015,14(2):171-177.
[8]YOONAH J,JIHYE M,JIWEON L,et al.Effects of different rootstocks on fruit quality of grafted pepper(Capsicum annuum L.)[J].Korean J Hortic Sci Technol,2013,31(6):687-699.
[9]KUBOTA C,Mc CLURE M A,KOKALIS-BURELLE N,et al.Vegetable grafting:history,use,and current technology status in North America[J].Hort Science,2008,43(6):1664-1669.
[10]胡艳青,苏媛,韩风叶,等.嫁接黄瓜在愈合过程中的解剖观察和抗氧化酶活性的变化研究[J].内蒙古农业大学学报,2007,28(3):224-230.HU Yanqing,SU Yuan,HAN Fengye,et al.The research of anatomical structures and three activity of antioxidases change of grafed cucumber seedling[J].J Inn Mong Agric Univ,2007,28(3):224-230.
[11]杨欢欢,李景富.不同砧木黄瓜/南瓜嫁接愈合过程的解剖学观察[J].北方园艺,2014(8):5-8.YANG Huanhuan,LI Jingfu.The anatomical observation of different root stock of healing process on the grafted cucumber/pumpkin[J].Northern Hortic,2014(8):5-8.
[12]黄坚钦,章滨森,陆建伟,等.山核桃嫁接愈合过程的解剖学观察[J].浙江林学院学报,2001,18(2):111-114.HUANG Jianqin,ZHANG Binsen,LU Jianwei,et al.Anatomical observation in graft union of Carya cathayenisis[J].J Zhejiang For Coll,2001,18(2):111-114.
[13]张红梅,丁明,姜武,等.不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究[J].园艺学报,2012,39(3):493-500.ZHANG Hongmei,DING Ming,JIANG Wu,et al.Histological and cytological research on graft union formation of grafted watermelon seedlings with different scion ages[J].Acta Hortic Sin,2012,39(3):493-500.
[14]朱晓慧,杨途熙,魏安智,等.无刺花椒嫁接愈合过程中相关生理指标的变化[J].西北林学院学报,2015,30(2):134-138.ZHU Xiaohui,YANG Tuxi,WEI Anzhi,et al.Changes of relative physiological indices involved in the graft union development of Zanthoxylum bungeanum[J].J Northwest For Univ,2015,30(2):134-138.
[15]蒋丽娟,马倩,佟金权,等.光皮树砧木生长指标及内源激素对嫁接成活率的影响[J].中南林业科技大学学报,2011,31(3):26-29.JIANG Lijuan,MA Qian,TONG Jinquan,et al.Effects of growth indices and endogenous hormone on grafting survival rate of Cornus wilsoniana[J].J Cent South Univ For Technol,2011,31(3):26-29.
[16]李锋.植物嫁接不亲和性的问题讨论[J].惠州学院学报(自然科学版),1991,17(4):170-172.LI Feng.Discussion on grafting incompatibility of plant[J].J Huizhou Univ Nat Sci,1991,17(4):170-172.
[17]CHINALIA F A,REGALI-SELEGHIN M H,CORREA E M.2,4-D toxicity:cause,effect and control[J].Terr Aquat Environ Toxicol,2007,1(2):24-33.
[18]高京草,王慧霞,李西选.可溶性蛋白、丙二醛含量与枣树枝条抗寒性的关系研究[J].北方园艺,2010(23):18-20.GAO Jingcao,WANG Huixia,LI Xixuan.Relationship between soluble protein,MDA and jujube tree cold hardiness[J].Northern Hortic,2010(23):18-20.
[19]商闯,马春红,翟彩霞,等.丙二醛(MDA)含量在玉米诱导抗病过程中的变化[J].华北农学报,2007,22(增刊):29-32.SHANG Chuang,MA Chunhong,ZHAI Caixia,et al.The changes of malondialdenyde content on course of the induced disease resistance[J].Acta Agric Boreali-Sin,2007,22(suppl):29-32.
[20]丁锦平,徐心诚,李文琦.番茄嫁接后生理指标的变化[J].北方园艺,2015(5):29-31.DING Jinping,XU Xincheng,LI Wenqi.Physiological changes of tomato after grafting[J].Northern Hortic,2015(5):29-31
[21]陈红,王永清,袁媛,等.茄子/番茄嫁接体发育过程中的蛋白质含量、POD、CAT和SOD活性及其同工酶研究[J].四川农业大学学报,2006,24(2):144-147.CHEN Hong,WANG Yongqing,YUAN Yuan,et al.Studies on protein content,activity and enzymes of POD,SOD and CAT in eggplant/tomato graft union[J].J Sichuan Agric Univ,2006,24(2):144-147.
[22]王超霞,邓嘉进,张雅丽,等.不同葡萄砧木对矢富罗莎果肉酚类化合物含量的影响[J].中外葡萄与葡萄酒,2011(7):28-31.WANG Chaoxia,DENG Jiajin,ZHANG Yali,et al.Influence of different grapevine rootstocks on the phenols content of Yatomi Rosa[J].Sino-Oversea Grape Wine,2011(7):28-31.
[23]张力平,孙长霞,李俊清,等.植物多酚的研究现状及发展前景[J].林业科学,2005,41(6):157-162.ZHANG Liping,SUN Changxia,LI Junqing,et al.The present conditions and development trend of plant polyphenols research[J].Sci Silv Sin,2005,41(6):157-162.
[24]郭大勇,谭琪,夏仁学.日本栗/板栗酚类物质含量与嫁接亲和力关系研究[J].华中农业大学学报,2004,23(3):341-343.GUO Dayong,TAN Qi,XIA Renxue.Studies on relationship between phenol content and graft compatibility in chestnut[J].J Huazhong Agric Univ,2004,23(3):341-343.
[25]马绍英,刘元卫,苏李维,等.葡萄离体嫁接部位的生理生化变化[J].果树学报,2016,33(2):179-186.MA Shaoying,LIU Yuanwei,SU Liwei,et al.Physiological and biochemical changes in the graft site after in vitro grafting in grape[J].J Fruit Sci,2016,33(2):179-186.
[26]雷东锋,蒋大宗,王一理.烟草中多酚氧化酶的生理生化特征及其活性控制的研究[J].西安交通大学学报,2003,37(12):1316-1320.LEI Dongfeng,JIANG Dazong,WANG Yili.Physiological and biochemical characteristic of tobacco polyphenol oxidase and control way for its activity[J].J Xi’an Jiaotong Univ,2003,37(12):1316-1320.
[27]陈静,符真珠,王政,等.牡丹多酚氧化酶的酶学特性[J].西北农林科技大学学报(自然科学版),2012,40(3):154-157.CHEN Jing,FU Zhenzhu,WANG Zheng,et al.Study on characteristics of polyphenol oxidase in peony[J].J Northwest A&F Univ Nat Sci Ed,2012,40(3):154-157.
[28]张淑红,王月,聂洪光,等.茄子嫁接愈合过程中相关酶活性的变化[J].沈阳农业大学学报,2016,47(3):266-270.ZHANG Shuhong,WANG Yue,NIE Hongguang,et al.Changes of related enzyme activity during healing process of grafted eggplant[J].J Shenyang Agric Univ,2016,47(3):266-270.
[29]严毅,高柱,何承忠,等.葡萄柚嫁接愈合过程关联酶活性研究进展[J].安徽农业科学,2011,39(2):734-736,742.YAN Yi,GAO Zhu,HE Chengzhong,et al.Research progress of related enzyme activity during graft healing process of grapefruit[J].J Anhui Agric Sci,2011,39(2):734-736,742.
[30]孙华丽,宋健坤,李鼎立,等.梨不同嫁接组合嫁接愈合过程中生理动态变化研究[J].北方园艺,2013(16):25-29.SUN Huali,SONG Jiankun,LI Dingli,et al.Study on the physiological dynamic change of different graft combinations in pear during the grafted healing process[J].Northern Horti,2013(16):25-29.
[31]曲云峰,赵忠,张小鹏.大扁杏嫁接愈合过程中几种生化物质含量的变化[J].西北农林科技大学学报(自然科学版),2008,36(5):73-78.QU Yunfeng,ZHAO Zhong,ZHANG Xiaopeng.The content changes of several biochemistry substances of grafted armeniaca surviving[J].J Northwest A&F Univ Nat Sci Ed,2008,36(5):73-78.
[32]张红梅,黄丹枫,丁明,等.不同苗龄接穗的西瓜嫁接体愈合过程中的3种酶活性变化[J].植物生理学通讯,2005,41(3):302-304.ZHANG Hongmei,HUANG Danfeng,DING Ming,et al.Changes in three enzyme activities in the prcess of watermelon seedlings grafted with different ages of scion[J].Plant Physiol Commun,2005,41(3):302-304.
[33]王瑞,陈永忠,王湘南,等.油茶芽苗砧嫁接愈合过程中砧穗相关生理指标的研究[J].西北农林科技大学学报(自然科学版),2014,42(1):46-50.WANG Rui,CHEN Yongzhong,WANG Xiangnan,et al.Dynamic changes in physiological indexes of Camellia oleifera nurse seed graft in the grafting and recovering process[J].J Northwest A&F Univ Nat Sci Ed,2014,42(1):46-50.
[34]程勋东,李姗姗,隋益虎,等.辣椒/茄子嫁接体保护酶同工酶分析[J].安徽科技学院学报,2015,29(3):14-17.CHENG Xundong,LI Shanshan,SUI Yihu,et al.Analysis of protecting isoenzymes of pepper/eggplant graft union[J].J Anhui Sci Technol Univ,2015,29(3):14-17.
[35]张子学,李爱青,许玉龙,等.西瓜嫁接苗的植物保护酶同工酶与亲和力分析[J].热带作物学报,2006,27(1):12-16.ZHANG Zixue,LI Aiqing,XU Yulong,et al.Analysis of plant protecting isozymes and theirs affinity of grafted watermelon seedlings[J].Chin J Trop Crops,2006,27(1):12-16.
[36]邰翔,郭世荣,陈志贵,等.以耐盐茄子为砧木的番茄嫁接苗的分析[J].江苏农业学报,2010,26(3):583-586.TAI Xiang,GUO Shirong,CHEN Zhigui,et al.Analysis of tomato seedlings grafted with salt tolerance eggplant rootstock[J].Jiangsu J Agric Sci,2010,26(3):583-586.
[37]冯金玲,杨志坚,陈辉.油茶芽苗砧嫁接体愈合过程AFLP分析[J].中南林业科技大学学报,2012,32(3):141-146.FENG Jinling,YANG Zhijian,CHEN Hui.AFLP analysis of nurse seed grafted union in Camellia oleifera during healing[J].J Cent South Univ For Technol,2012,32(3):141-146.
[38]YIN Hao,YAN Bo,SUN Jing,et al.Graft-union development:a delicate process that involves cell-cell communication between scion and stock for local auxin accumulation[J].J Exp Bot,2012,63(11):4219-4232.
[39]QIU Lingling,JIANG Bo,FANG Jia,et al.Analysis of transcriptome in hickory(Carya cathayensis),and uncover the dynamics in the hormonal signaling pathway during graft process[J].BMC Genom,2016,17(1):935.doi:10.1186/s12864-016-3182-4.
[40]COOKSON S J,MORENO M J C,HEVIN C,et al.Graft union formation in grapevine induces tran scriptional changes related to cell wall modification,wounding,hormone signalling,and secondary metabolism[J].J Exp Bot,2013,64(10):2997-3008.
[41]田晓彬,齐红岩,李岩,等.不同砧木嫁接对薄皮甜瓜成熟期品质、香气合成相关酶活性及基因表达的影响[J].中国农业科学,2012,45(9):1766-1774.TIAN Xiaobin,QI Hongyan,LI Yan,et al.Effects of different rootstocks on ripening quality,activities and gene expression of aroma-related enzymes in grafting oriental melon(Cucumis melo var.makuwa Makino)[J].Sci Agric Sin,2012,45(9):1766-1774.
[42]AYRE B G,TURGEON R.Graft transmission of a floral stimulant derived from constans[J].Plant Physiol,2004,135(4):2271-2278.
[43]LIU Na,YANG Jinghua,GUO Shaogui,et al.Genome-Wide identification and comparative analysis of conserved and novel micro RNAs in grafted watermelon by high-throughput sequencing[J].PLo S One,2013,8(2):e57359.doi:10.1371/journal.pone.0057359
[44]YEOMAN M M,KILPATRICK D C,MIEDZYBRODZKA M B,et al.Cellular interactions during graft formation in plants,a recognition phenomenon?[J].Symp Soc Exp Biol,1978,32(3):139-160.
[45]曾义安,朱月林,黄保健,等.嫁接黄瓜的光合特性及叶片激素含量和可溶性蛋白研究[J].南京农业大学学报,2005,28(1):16-19.ZENG Yian,ZHU Yuelin,HUANG Baojian,et al.Study on photosynthetic characteristics,GA and ABA contents,and soluble proteins in leaves of grafted cucumber[J].J Nanjing Agric Univ,2005,28(1):16-19.
[46]李跃建,梁根云,刘小俊,等.黄瓜嫁接苗和自根苗的蛋白质组学研究[J].园艺学报,2009,36(8):1147-1152.LI Yuejian,LIANG Genyun,LIU Xiaojun,et al.Proteomic study on grafted and non-grafted cucumber(Cucumis sativus L.)[J].Acta Hortic Sin,2009,36(8):1147-1152.
[47]XU Qing,GUO Shirong,LI Lin,et al.Proteomics analysis of compatibility and incompatibility in grafted cucumber seedlings[J].Plant Physiol Biochem,2016,105(4):21-28.doi:10.1016/j.plaphy.2016.04.001
[48]冯金玲,杨志坚,陈辉.油茶芽苗砧嫁接口不同发育时期差异蛋白质分析[J].应用生态学报,2012,23(8):2055-2061.FENG Jinling,YANG Zhijian,CHEN Hui.Analysis of differential proteins in nurse seed grafted unions of Camellia oleifera at its different developmental stages[J].Chin J Appl Ecol,2012,23(8):2055-2061.
[49]ODA M,MARUYAMA M,MORI G.Water transfer at graft union of tomato plants grafted onto solanum rootstocks[J].Engei Gakkai Zasshi,2015,74(6):458-463.
[50]刘传荷,洪健,夏国华,等.山核桃嫁接过程中愈伤反应的细胞学观察[J].林业科学,2009,45(6):34-38.LIU Chuanhe,HONG Jian,XIA Guohua,et al.Cytological observation on healing responses in grafting of Carya cathayensis[J].Sci Silv Sin,2009,45(6):34-38.
[51]GISBERT C,PROHENS J,RAIG魷N M D,et al.Eggplant relatives as sources of variation for developing new rootstocks:effects of grafting on eggplant yield and fruit apparent quality and composition[J].Sci Hortic,2001,128(1):14-22.
[52]张帆,贾长才,姜立纲,等.黄瓜专用砧木品种京欣砧5号的选育[J].中国蔬菜,2011(4):73-75.ZHANG Fan,JIA Changcai,JIANG Ligang,et al.A new cucumber rootstock F1 hybrid-‘Jingxinzhen No.5’[J].China Veget,2011(4):73-75.
[53]施先锋,李爱成,李煜华,等.西瓜专用砧木:鄂砧1号葫芦的选育[J].长江蔬菜,2012(6):17-19.SHI Xianfeng,LI Aicheng,LI Yuhua,et al.Breeding of a new watermelon rootstock cultivar,E-zhen No.1[J].J Changjiang Veget,2012(6):17-19.
[54]潘云平,张西森,王迎春,等.不同砧木嫁接西红柿亲和性试验研究[J].现代农业科技,2012(10):109-110.PAN Yunping,ZHANG Xisen,WANG Yingchun,et al.Study on compatibility of grafted tomatoes with different rootstocks[J].Mod Agric Technol,2012(10):109-110.
[55]VU N T,XU Zhihao,KIM Y S,et al.Effect of nursery environmental condition and different cultivars on survival rate of grafted tomato seedling[J].Acta Hortic,2014,1037(2):765-770.
[56]KAWAGUCHI M,TAJI A,BACKHOUSE D,et al.Anatomy and physiology of graft incompatibility in solanaceous plants[J].J Hortic Sci Biotechnol,2008,83(5):581-588.
[57]王白坡,程晓建,喻卫武.山核桃嫁接育苗成活率探讨[J].浙江林学院学报,2002,19(3):231-234.WANG Baipo,CHENG Xiaojian,YU Weiwu.Inquiry into survival rate of Carya cathayensis seedlings in grafting[J].J Zhejiang For Coll,2002,19(3):231-234.
[58]胡靖楠,蒋细春,林跃,等.化香作砧木嫁接山核桃育苗及造林效果[J].湖南林业科技,2009,36(5):11-14.HU Jingnan,JIANG Xichun,LIN Yue,et al.Effect of grafting Carya cathayenisis onto Platycarya strobilaceab on seedling-raising and planting[J].J Hunan For Sci Technol,2009,36(5):11-14.
[59]MUNEER S,KO C H,SOUNDARARAJAN P,et al.Proteomic study related to vascular connections in watermelon scions grafted onto bottle-gourd rootstock under different light intensities[J].PLo S One,2015,1 0(3):e0120899.doi:10.1371/journal.pone.0120899.
[60]宮本眞吾.LED照明を用いた物工場[J].表面技術,2010,61(9):641-645.MIYAMOTO S.Advances in plant factories with LED lighting[J].J Surf Finish Soc Jpn,2010,61(9):641-645.
[61]LEE K M,LIM C S,MUNEER S,et al.Functional vascular connections and light quality effects on tomato grafted unions[J].Sci Hortic,2016,201:306-317.doi:org/10.1016/j.scienta.2016.02013.
[62]赵锋亮.长期夜间亚低温和长期昼间亚高温对茄子生长发育的影响[D].郑州:河南农业大学,2008.ZHAO Fengliang.Effects of Long Team Night Sub-low Temperature and Long Team Day Sub-high Temperature on Eggplant Growth and Development[D].Zhengzhou:Henan Agricultural University,2008.
[63]王利民,王献革,李军英,等.观赏植物火鹤、红宝石喜林芋的快速繁殖[J].华北农学报,2002,17(增刊):213-218.WANG Limin,WANG Xiange,LI Junying,et al.Tissue cluture and rapid propagation of Anthurium scherzrianum and Philodendron enbesoens cv Red Emerald of modern ornamental aroid[J].Acta Agric Boreal-Sin,2002,17(supp1):213-218.
[64]李梅,须晖,李天来,等.不同夜温对番茄叶片呼吸及光合启动时间的影响[J].石河子大学学报(自然科学版),2006,24(2):201-204.LI Mei,XU Hui,LI Tianlai,et al.Influence of different night temperature the starting time of photosynthesis to tomato leaves[J].J Shihezi Univ Nat Sci,2006,24(2):201-204.
[65]赵渊渊,董春娟,尚庆茂.夜温对番茄套管嫁接苗愈合的影响[J].西北植物学报,2015,35(3):493-499.ZHAO Yuanyuan,DONG Chunjuan,SHANG Qingmao,et al.Healing responses of tube grafted tomato plug seedling under different night temperatures[J].Acta Bot Boreal-Occident Sin,2015,35(3):493-499.
[66]于金超,辜松,陈克农.利用小拱棚愈合嫁接苗的试验研究[J].农机化研究,2008(1):154-155.YU Jinchao,GU Song,CHEN Kenong.A study on the small arch awning of grafting seedling for healing[J].J Agric Mechan Res,2008(1):154-155.
[67]SHIBUYA T,ITAGAKI K,WANG Yu,et al.Grafting transiently suppresses development of powdery mildew colonies,probably through a quantitative change in water relations of the host cucumber scions during graft healing[J].Sci Hortic,2015,192:197-199.doi:org/10.1016/j.scienta.2015.06.010.
[68]MUN B,JANG Y,GOTO E,et al.Measurement system of whole-canopy carbon dioxide exchange rates in grafted cucumber transplants in which scions were exposed to different water regimes using a semi-open multi-chamber[J].Sci Hortic,2011,130(3):607-614.doi:org/10.1016/j.scienta.2011.08.017.
[69]郑碧娟,苏丽芳,陈世品,等.外源激动素对油茶芽苗砧嵌合体愈合的效应[J].福建农林大学学报(自然科学版),2014,43(2):146-150.ZHENG Bijuan,SU Lifang,CHEN Shipin,et al.Effects of exogenous KT on the healing of the grafted union of Camelia oleifera nurse seed[J].J Fujian Agric For Univ Nat Sci Ed,2014,43(2):146-150.
[70]赵琳,黄钰,李甜江,等.云油茶5个品种芽苗砧嫁接技术研究[J].西部林业科学,2012,41(5):19-25.ZHAO Lin,HUANG Yu,LI Tianjiang,et al.Study on seedling stock grafting of five varieties of‘Yunyou’series of Camellia oleifera[J].J West China For Sci,2012,41(5):19-25.
[71]GROSSMANN K.Auxin herbicide action:lifting the veil step by step[J].Plant Sign Behav,2007,2(5):421-423.