血小板膜糖蛋白GP Ibα-145C/T单核苷酸多态性与大肠癌的相关性研究
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摘要
目的探讨血小板膜糖蛋白(GP)Ibα-145C/T单核苷酸多态性与大肠癌的发生及发展的相关性。方法采用病例对照研究,用聚合酶链反应—序列特异性引物PCR(PCR-SSP)技术和琼脂糖凝胶电泳技术,对大肠癌患者及健康体检者各140例外周血标本进行GP Ibα-145C/T位点基因分型检测。结果 (1)大肠癌组GP Ibα-145基因型和等位基因的频率分布与正常对照组比较,差异具有统计学意义(P<0.05);(2)大肠癌组GP Ibα-145C/T位点基因型及等位基因频率在大肠癌TNM分期中的比较,差异无统计学意义(P>0.05);(3)GP Ibα-145C/T中携带CT基因型的个体大肠癌发病率是携带CC基因型个体的3.232倍,携带T等位基因的个体大肠癌发病率是携带C等位基因个体的2.610倍。结论 GP Ibα-145C/T基因多态性可能与大肠癌的发生有关。
Objective To investigate the correlation between the occurrence and development of platelet membrane glycoprotein(PMGP)Ibα-145 C/T single nucleotide polymorphisms and colorectal cancer.Methods Used casecontrol study,polymerase chain reaction sequence specific primer PCR(PCR-SSP)technology and agarose gel electrophoresis technology,the GP Ibα-145 C/T genotyping was performed on 140 peripheral blood samples of colorectal cancer patients and health people.Results The distribution ofGP Ibα-145 C/T genotype and allele frequencies between colorectal cancer group and control group were significantly different(P<0.05).The frequency of GP Ibα-145 C/T genotype and allele frequencies in colorectal cancer group were not significantly different between the TNM stages of colorectal cancer(P>0.05).The incidence of individual colorectal cancer with CT genotype in GP Ibα-145 C/T was 3.232 times that of the individual with CC genotype,the incidence of individual colorectal cancer with T allele was2.610 times that of individuals with C allele.Conclusion GP Ibα-145 C/T genetic polymorphism might associate with the occurrence of colorectal cancer.
Objective To investigate the correlation between the occurrence and development of platelet membrane glycoprotein(PMGP)Ibα-145 C/T single nucleotide polymorphisms and colorectal cancer.Methods Used casecontrol study,polymerase chain reaction sequence specific primer PCR(PCR-SSP)technology and agarose gel electrophoresis technology,the GP Ibα-145 C/T genotyping was performed on 140 peripheral blood samples of colorectal cancer patients and health people.Results The distribution ofGP Ibα-145 C/T genotype and allele frequencies between colorectal cancer group and control group were significantly different(P<0.05).The frequency of GP Ibα-145 C/T genotype and allele frequencies in colorectal cancer group were not significantly different between the TNM stages of colorectal cancer(P>0.05).The incidence of individual colorectal cancer with CT genotype in GP Ibα-145 C/T was 3.232 times that of the individual with CC genotype,the incidence of individual colorectal cancer with T allele was2.610 times that of individuals with C allele.Conclusion GP Ibα-145 C/T genetic polymorphism might associate with the occurrence of colorectal cancer.
引文
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[2]姚兴伟,李娟,杜志刚,等.大肠癌临床病理特征与血小板增多关系的探讨[J].中国肿瘤临床,2007,34(8):450-453.
[3]黄传钱,赖晓兰,肖吝生,等.血小板升高与肠癌肝转移的关系研究[J].牡丹江医学院学报,2016,37(5):121-123+151.
[4]冯志平,李积德,李进章,等.青海地区大肠癌患者血小板增高的临床研究[J].河南医学研究,2008,17(3):221-224.
[5]Laublin,Borsing L.Selections promote tumor metastasis[J].Semin Cancer Biol,2010,20(3):169-177.
[6]Jain S,Zuka M,Liu J,et al.Platelet glycoprotein Ib alpha supports experimental lung metastasis[J].Proceedings of the National Academy of Science of the United States of America,2007,104(21):9024-9028.
[7]李映峰,徐建,周学录,等.大肠癌发病危险因素研究(附98例报告)[J].贵州医药,2014,38(5):409-410.
[8]杨志伟,王燕锋,朱大勇,等.血小板源性生长因子-D基因多态性与大肠癌遗传易感性关系的研究[J].医学研究杂志,2014,43(9):60-63.
[9]曹玉珠,刘兆国,单云龙,等.血小板介导肿瘤血行转移的作用及其机制研究进展[J].中国药理学通报,2015,31(2):166-169.
[10]刘艳兰,李菊香,龚皓.血小板增多与肿瘤的关系[J].中国处方药,2014,12(10):86.
[11]Simanek R,Vormittagv R,Ay C,et al.High platelet count associated with venous thromb-oembolism in cancer patients:results from the Vienna Cancer and Thrombosis Study(CATS)[J].Journal of Thrombosis and Haemostasis,2010,8(1):114-120.
[12]Labelle M,Begum S,Hynes RO.Direct signaling between platelets and cancer cells induces an epithelialmesenchymal-like transition and promotes metastasis[J].Cancer Cell,2013,20(5):451-456.
[13]Alonso Escolano D,Strongin AY,Chung AW,et al.Membrane type-1matrix metalloproteinase stimulates tumour cell-induced platelet aggregation:role of receptor glycoproteins[J].British Journalof Pharmacology,2004,141(2):241-252.
[14]陈涵薇,任明,张浩,等.武汉地区人群血小板特异性基因(HPA-1~17)的多态性分析[J].临床血液学杂志,2014,27(8):634-637.
[15]夏兰,蔡兴权,陈鑫萍,等.海南岛黎族人血小板1~17抗原系统基因多态性研究[J].中华医学遗传学杂志,2010,27(2):229-230.
[16]何智,乔艳辉.乌鲁木齐地区哈萨克族献血人群HPA-1~17基因多态性分析[J].中国输血杂志,2016,29(8):798-801.
[17]尹红,杨芳,杨晋渝,等.贵州地区随机人群血小板抗原基因频率调查和不配合率分析[J].中国输血杂志,2015,28(3):279-282.