内生真菌多糖浸种对苦荞出苗及幼苗素质的影响
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摘要
以川荞1号和西荞1号为试验材料,研究了内生真菌多糖(0、100、200、400、800 mg/kg)浸种对苦荞出苗和幼苗素质的影响。结果表明:内生真菌多糖浸种显著提高了川荞1号和西荞1号的出苗率,以100 mg/kg处理最高,比对照分别提高16.0%和17.4%。苦荞幼苗性状方面,内生菌多糖浸种显著增加了植株高度、第一节间长度和单株叶面积,两个品种表现趋势相同。浸种对苦荞幼苗各器官(根、茎和叶)的干物质重和根冠比有显著影响,均随着浓度的增加呈先升高后降低的变化趋势,以100 mg/kg浓度处理最高,两个品种表现趋势一致。苦荞幼苗的根系活性和SPAD值也以100 mg/kg处理最高,分别比对照高31.6%～34.9%和3.1%～3.6%。综合可知,采用100 mg/kg内生真菌多糖浸种最有利于提高苦荞出苗率和幼苗素质。
The Chuanqiao1 and Xiqiao1 cultivars were used to investigate the effect of seed soaked bypolysaccharide (0, 100, 200, 400, 800 mg/kg) obtained from endophytic fungus on the sprout and seedling qualityof tartary buckwheat. The results showed that seed soaked by polysaccharide could improve the germination rateof Chuanqiao1 and Xiqiao1 cultivars. The treatment of 100 mg/kg was the highest, which was 16.0% and 17.4%significantly higher than CK, respectively. Seed soaked by polysaccharide significantly increased the plant height,the 1st internode length and leaf area per plant, and two cultivars showed the same trend. The root activity and SPADvalue of the treatment of 100 mg/kg were the highest among all treatments, which were 31.6%~34.9% and 3.1~3.6%significantly higher than other treatments, respectively. Above all, the agricultural measure of seed soaked bypolysaccharide (100 mg/kg) was beneficial to improve the germination rate and seedling quality of tartary buckwheatin this experiment.
The Chuanqiao1 and Xiqiao1 cultivars were used to investigate the effect of seed soaked bypolysaccharide (0, 100, 200, 400, 800 mg/kg) obtained from endophytic fungus on the sprout and seedling qualityof tartary buckwheat. The results showed that seed soaked by polysaccharide could improve the germination rateof Chuanqiao1 and Xiqiao1 cultivars. The treatment of 100 mg/kg was the highest, which was 16.0% and 17.4%significantly higher than CK, respectively. Seed soaked by polysaccharide significantly increased the plant height,the 1st internode length and leaf area per plant, and two cultivars showed the same trend. The root activity and SPADvalue of the treatment of 100 mg/kg were the highest among all treatments, which were 31.6%~34.9% and 3.1~3.6%significantly higher than other treatments, respectively. Above all, the agricultural measure of seed soaked bypolysaccharide (100 mg/kg) was beneficial to improve the germination rate and seedling quality of tartary buckwheatin this experiment.
引文
[1]赵钢,陕方.中国苦荞[M].北京:科学出版社,2009:1-4.
[2]Ohmi O, Mitsuyuki T. Distribution of cultivated and wild buckwheat species in the Nu river valley of southwestern China[J]. Fagopyrum,2005(22):1-5.
[3]冯佰利,姚爱华,高金峰,等.中国荞麦优势区域布局与发展研究[J].中国农学通报,2005,21(3):375-377.
[4]向达兵,彭镰心,赵钢,等.荞麦栽培研究进展[J].作物杂志,2013(3):1-6.
[5]陈慧,李建婷,秦丹.苦荞的保健功效及开发利用研究进展[J].农产品加工, 2016(15):63-66.
[6]赵钢,邹亮.荞麦的营养与功能[M].北京:科学出版社,2012:1-12.
[7]郑亭,樊高琼,王秀芳,等.耕作方式、播深及覆土对机播套作小麦麦苗素质的影响[J].农业工程学报,2011,27(5):164-168.
[8]李长亮,李昌远,魏世杰,等.不同播量与肥料对苦荞菜产量及出苗率的影响[J].现代农业科技,2014(22):73.
[9]闫艳红,杨文钰,张新全,等.套作遮荫条件下烯效唑对大豆壮苗机理的研究[J].中国油料作物学报,2011,33(3):259-264.
[10]贾春林,郭洪海,袁奎明,等.黄淮区不同播种方式对玉米出苗质量和土壤环境的影响[J].玉米科学,2012,20(1):108-111.
[11]莫亿伟,王忠,梁国斌,等.施氮处理对水稻子代乳苗素质的影响[J].作物学报,2004,30(3):227-231.
[12]段里成,张坤,章起明,等.低温胁迫对直播早稻出苗及幼苗生长的影响[J].广东农业科学,2018,45(3):1-6.
[13]焦朋娜,段进潮,任跃英,等.野生抚育山参内生真菌分离鉴定[J].菌物研究,2012,10(1):36-40.
[14]刘丽莉,吕国忠,孙晓东.林木内生真菌研究进展[J].菌物研究,2006,4(2):54-59.
[15]王娜,杨镇,曹君,等.植物内生菌提取物对水稻生长发育的影响[J].吉林农业大学学报,2014,36(1):10-16.
[16]马晓颖,杨镇,宋艳雨,等.不同微生物多糖对植物生长和植保素累积的影响[J].辽宁农业科学,2016(2):23-26.
[17]Zhao G, Zhao J L, Peng L X, et al. Effects of yeast polysaccharide on growth and flavonoid accumulation in fagopyrum tataricum sprout cultures[J]. Molecules, 2012,17(10):11335-11345.
[18]Zhong L Y, Niu B, Tang L, et al. Effects of polysaccharideelicitorsfromendophytic fusariumoxysporumFat9onthegrowth,flavonoid accumulation and antioxidant Property of Fagopyrum tataricum sprout cultures[J].Molecules, 2016, 21(12):1590-1602.
[19]Zhong L Y, Zou L, Tang X H, et al. Community of endophytic fungi from the medicinal and edible plant Fagopyrum tataricum and their antimicrobial activity[J]. Tropical Journal of Pharmaceutical Research February, 2017, 16(2):387-396.
[20]周秋艳,卿朕,骆海玉,等.两株广西地不容内生真菌的抑菌活性研究[J].广东农业科学,2016,43(4):111-116.
[21]鲍士旦.土壤农化分析[M].第3版.北京:中国农业出版社,2000:25-109.
[22]张志良,霍伟箐.植物生理学实验指导[M].北京:高等教育出版社,2003:127-128.
[23]杨文钰,屠乃美.作物栽培学各论[M].北京:中国农业出版社,2003:88-90.
[24]贾春林,郭洪海,袁奎明,等.黄淮区不同播种方式对玉米出苗质量和土壤环境的影响[J].玉米科学,2012,20(1):108-111.
[25]郭志欣,顾地周,黄芳芳,等.五味子药渣多糖提取及其对白菜种子萌发活性影响的研究[J].种子,2013,32(2):24-27.
[26]谢冬娣,石贵玉,岳君.壳聚糖对苦瓜种子萌发和幼苗生长的影响[J].广东农业科学,2006(6):33-35.
[27]姜虎生,石德成.壳聚糖对白菜种子萌发和幼苗生长的影响[J].河南师范大学学报,2006(2):19-23.
[28]闫艳红,杨文钰,张新全,等.套作遮荫条件下烯效唑对大豆壮苗机理的研究[J].中国油料作物学报,2011,33(3):259-264.
[29]张凯.黄瓜穴盘苗成苗标准和质量指标研究[D].北京:中国农业大学,2005.
[30]郑亭,樊高琼,王秀芳,等.耕作方式、播深及覆土对机播套作小麦麦苗素质的影响[J].农业工程学报,2011,27(5):164-168.
[31]刘俊仙,李松,谭芳,等.多效唑浸种对甘蔗幼苗分蘖发生及生理特性变化的影响[J].广东农业科学,2016,43(4):38-43.
[32]黄诗宸,王清,张文平,等.乳酸菌多糖对水稻种子萌发及幼苗生长的影响[J].江西农业学报,2018,30(4):59-62.
[33]朱云林,顾大路,王伟中,等.壳聚糖对水稻幼苗抗冷性的影响[J].江苏农业科学,2017,45(8):66-68.
[34]赵俞,王琦.人参内生菌的多样性及其次生代谢产物[J].菌物研究,2012,10(2):113-118.
[35]杨娟.香菇柄水提液对油菜种子活力及抗逆性的影响[D].杨凌:西北农林科技大学,2013.
[36]毕锐,崔娟,吴磊,等.昆虫多糖对大豆生长调节作用及产量的影响[J].吉林农业大学学报,2015,37(4):402-405.
[2]Ohmi O, Mitsuyuki T. Distribution of cultivated and wild buckwheat species in the Nu river valley of southwestern China[J]. Fagopyrum,2005(22):1-5.
[3]冯佰利,姚爱华,高金峰,等.中国荞麦优势区域布局与发展研究[J].中国农学通报,2005,21(3):375-377.
[4]向达兵,彭镰心,赵钢,等.荞麦栽培研究进展[J].作物杂志,2013(3):1-6.
[5]陈慧,李建婷,秦丹.苦荞的保健功效及开发利用研究进展[J].农产品加工, 2016(15):63-66.
[6]赵钢,邹亮.荞麦的营养与功能[M].北京:科学出版社,2012:1-12.
[7]郑亭,樊高琼,王秀芳,等.耕作方式、播深及覆土对机播套作小麦麦苗素质的影响[J].农业工程学报,2011,27(5):164-168.
[8]李长亮,李昌远,魏世杰,等.不同播量与肥料对苦荞菜产量及出苗率的影响[J].现代农业科技,2014(22):73.
[9]闫艳红,杨文钰,张新全,等.套作遮荫条件下烯效唑对大豆壮苗机理的研究[J].中国油料作物学报,2011,33(3):259-264.
[10]贾春林,郭洪海,袁奎明,等.黄淮区不同播种方式对玉米出苗质量和土壤环境的影响[J].玉米科学,2012,20(1):108-111.
[11]莫亿伟,王忠,梁国斌,等.施氮处理对水稻子代乳苗素质的影响[J].作物学报,2004,30(3):227-231.
[12]段里成,张坤,章起明,等.低温胁迫对直播早稻出苗及幼苗生长的影响[J].广东农业科学,2018,45(3):1-6.
[13]焦朋娜,段进潮,任跃英,等.野生抚育山参内生真菌分离鉴定[J].菌物研究,2012,10(1):36-40.
[14]刘丽莉,吕国忠,孙晓东.林木内生真菌研究进展[J].菌物研究,2006,4(2):54-59.
[15]王娜,杨镇,曹君,等.植物内生菌提取物对水稻生长发育的影响[J].吉林农业大学学报,2014,36(1):10-16.
[16]马晓颖,杨镇,宋艳雨,等.不同微生物多糖对植物生长和植保素累积的影响[J].辽宁农业科学,2016(2):23-26.
[17]Zhao G, Zhao J L, Peng L X, et al. Effects of yeast polysaccharide on growth and flavonoid accumulation in fagopyrum tataricum sprout cultures[J]. Molecules, 2012,17(10):11335-11345.
[18]Zhong L Y, Niu B, Tang L, et al. Effects of polysaccharideelicitorsfromendophytic fusariumoxysporumFat9onthegrowth,flavonoid accumulation and antioxidant Property of Fagopyrum tataricum sprout cultures[J].Molecules, 2016, 21(12):1590-1602.
[19]Zhong L Y, Zou L, Tang X H, et al. Community of endophytic fungi from the medicinal and edible plant Fagopyrum tataricum and their antimicrobial activity[J]. Tropical Journal of Pharmaceutical Research February, 2017, 16(2):387-396.
[20]周秋艳,卿朕,骆海玉,等.两株广西地不容内生真菌的抑菌活性研究[J].广东农业科学,2016,43(4):111-116.
[21]鲍士旦.土壤农化分析[M].第3版.北京:中国农业出版社,2000:25-109.
[22]张志良,霍伟箐.植物生理学实验指导[M].北京:高等教育出版社,2003:127-128.
[23]杨文钰,屠乃美.作物栽培学各论[M].北京:中国农业出版社,2003:88-90.
[24]贾春林,郭洪海,袁奎明,等.黄淮区不同播种方式对玉米出苗质量和土壤环境的影响[J].玉米科学,2012,20(1):108-111.
[25]郭志欣,顾地周,黄芳芳,等.五味子药渣多糖提取及其对白菜种子萌发活性影响的研究[J].种子,2013,32(2):24-27.
[26]谢冬娣,石贵玉,岳君.壳聚糖对苦瓜种子萌发和幼苗生长的影响[J].广东农业科学,2006(6):33-35.
[27]姜虎生,石德成.壳聚糖对白菜种子萌发和幼苗生长的影响[J].河南师范大学学报,2006(2):19-23.
[28]闫艳红,杨文钰,张新全,等.套作遮荫条件下烯效唑对大豆壮苗机理的研究[J].中国油料作物学报,2011,33(3):259-264.
[29]张凯.黄瓜穴盘苗成苗标准和质量指标研究[D].北京:中国农业大学,2005.
[30]郑亭,樊高琼,王秀芳,等.耕作方式、播深及覆土对机播套作小麦麦苗素质的影响[J].农业工程学报,2011,27(5):164-168.
[31]刘俊仙,李松,谭芳,等.多效唑浸种对甘蔗幼苗分蘖发生及生理特性变化的影响[J].广东农业科学,2016,43(4):38-43.
[32]黄诗宸,王清,张文平,等.乳酸菌多糖对水稻种子萌发及幼苗生长的影响[J].江西农业学报,2018,30(4):59-62.
[33]朱云林,顾大路,王伟中,等.壳聚糖对水稻幼苗抗冷性的影响[J].江苏农业科学,2017,45(8):66-68.
[34]赵俞,王琦.人参内生菌的多样性及其次生代谢产物[J].菌物研究,2012,10(2):113-118.
[35]杨娟.香菇柄水提液对油菜种子活力及抗逆性的影响[D].杨凌:西北农林科技大学,2013.
[36]毕锐,崔娟,吴磊,等.昆虫多糖对大豆生长调节作用及产量的影响[J].吉林农业大学学报,2015,37(4):402-405.