DNA条形码技术在蚊虫分类鉴定中的研究进展
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摘要
DNA条形码在物种分类鉴定中具有快速、准确的特点。近年来,基于线粒体细胞色素c氧化酶亚基Ⅰ(cytochrome c oxidase subunitⅠ,COⅠ)基因的DNA条形码技术已成功应用于蚊虫物种水平的鉴定。本文主要对DNA条形码技术及原理,基于COⅠ基因的DNA条形码技术对蚊虫种和种团鉴定的应用及其局限性,以及基于COⅡ、16S RNA、核糖体DNA内转录间隔区(ribosome DNA internal transcribed spacer,r DNA-ITS)中的第一内转录间隔区(the first internal transcribed spacer,ITS1)和第二内转录间隔区(the second internal transcribed spacer,ITS2)等其他分子标记在蚊虫分类鉴定中的应用及其辅助COⅠ基因对蚊虫进行分类鉴定的研究进展进行综述。
DNA barcoding technique is a fast and accurate method for species identification. The DNA barcoding based on cytochrome c oxidase subunit Ⅰ(CO Ⅰ) has recently been successfully applied for species identification of Culicidae. In this paper, we introduce the technique and principle of DNA barcoding, application and limitation of the technique based on CO I gene for species identification, as well as research development on applications of other molecular makers such as CO Ⅱ, 16 S RNA, and the first and the second internal transcribed spacers(ITS1 and ITS2) in species identification and to assist the COⅠ gene in identifying Culicidae species.
DNA barcoding technique is a fast and accurate method for species identification. The DNA barcoding based on cytochrome c oxidase subunit Ⅰ(CO Ⅰ) has recently been successfully applied for species identification of Culicidae. In this paper, we introduce the technique and principle of DNA barcoding, application and limitation of the technique based on CO I gene for species identification, as well as research development on applications of other molecular makers such as CO Ⅱ, 16 S RNA, and the first and the second internal transcribed spacers(ITS1 and ITS2) in species identification and to assist the COⅠ gene in identifying Culicidae species.
引文
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[3]Govindarajan M,Rajeswary M,Muthukumaran U,et al.Singlestep biosynthesis and characterization of silver nanoparticles using Zornia diphylla leaves:a potent eco-friendly tool against malaria and arbovirus vectors[J].J Photochem Photobiol B,2016,161:482-489.
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[5]付文博,陈斌.中国蚊科分类研究新进展[C].重庆市昆虫学会2013年学术交流及研讨会论文集,2013:149-164.
[6]Wang G,Li C,Guo X,et al.Identifying the main mosquito species in China based on DNA barcoding[J].PLo S One,2012,7(10):e47051.
[7]中国动物志昆虫纲第8卷双翅目蚊科(上)[M].北京:科学出版社,1997:25.
[8]Krtini B,Ludo ki J,Milankov V.Multi-character approach reveals a discordant pattern of phenotypic variation during ontogeny in Culex pipiens biotypes(Diptera∶Culicidae)[J].Bull Entomol Res,2015,105(1):129-138.
[9]Wong WH,Tay YC,Puniamoorthy J,et al.‘Direct PCR’optimization yields a rapid,cost-effective,nondestructive and efficient method for obtaining DNA barcodes without DNA extraction[J].Mol Ecol Resour,2014,14(6):1271-1280.
[10]钟敏,蔺应学,王英.分子生物学技术在按蚊近缘种鉴定的应用进展[J].中国热带医学,2014,14(12):1529-1532.
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[12]Hebert PD,Cywinska A,Ball SL,et al.Biological identifications through DNA barcodes[J].Proc Biol Sci,2003,270(1512):313-321.
[13]薛银刚,许霞,蔡焕兴,等.DNA条形码技术在水生生物分类中的研究进展[J].环境监控与预警,2012,4(6):23-26.
[14]Versteirt V,Nagy ZT,Roelants P,et al.Identification of Belgian mosquito species(Diptera∶Culicidae)by DNA barcoding[J].Mol Ecol Resour,2015,15(2):449-457.
[15]Rajavel AR,Kumar NP,Natarajan R,et al.Morphological and molecular characterization of the ecological,biological and behavioural variants of the JE vector Culex tritaeniorhynchus:an assessment of its taxonomic status[J].J Vector Borne Dis,2015,52(1):40-51.
[16]Rozo-Lopez P,Mengual X.Mosquito species(Diptera∶Culicidae)in three ecosystems from the Colombian Andes:identification through DNA barcoding and adult morphology[J].Zookeys,2015,(513):39-64.
[17]Wang Y,Xu J,Ma Y.Molecular characterization of cryptic species of Anopheles barbirostris van der Wulp in China[J].Parasit Vectors,2014,7:592.
[18]Lehr MA,Kilpatrick CW,Wilkerson RC,et al.Cryptic species in the Anopheles(Nyssorhynchus)albitarsis(Diptera∶Culicidae)complex:incongruence between random amplified polymorphic DNA-polymerase chain reaction identification and analysis of mitochondrial DNA COⅠgene sequences[J].Ann Entomol Soc Am,2005,98(6):908-917.
[19]Remigio EA,Hebert PD.Testing the utility of partial COⅠsequences for phylogenetic estimates of gastropod relationships[J].Mol Phylogenet Evol,2003,29(3):641-647.
[20]江颖,张仪,郭云海.DNA条形码技术在软体动物分类学中的研究进展[J].中国寄生虫学与寄生虫病杂志,2016,34(1):80-83.
[21]Di Pinto A,Marchetti P,Mottola A,et al.Species identification in fish fillet products using DNA barcoding[J].Fish Res,2015,170:9-13.
[22]Osterhage D,Pogonoski JJ,Appleyard SA,et al.Integrated taxonomy reveals hidden diversity in Northern Australian fishes:a new species of Seamoth(Genus Pegasus)[J].PLo S ONE,2016,11(3):e0149415.
[23]Balasubramanian CP,Cubelio SS,Mohanlal DL,et al.DNA sequence information resolves taxonomic ambiguity of the common mud crab species(Genus Scylla)in Indian waters[J].Mitochondrial DNA A DNA Mapp Seq Anal,2016,27(1):270-275.
[24]Jordaens K,Goergen G,Virgilio M,et al.DNA Barcoding to improve the taxonomy of the afrotropical hoverflies(Insecta∶Diptera∶Syrphidae)[J].PLo S One,2015,10(10):e0140264.
[25]Wen J,Zeng L,Sun Y,et al.Authentication and traceability of fish maw products from the market using DNA sequencing[J].Food Control,2015,55:185-189.
[26]刘勇,宋毓,李晓宇.基于线粒体COⅠ基因的DNA条形码技术在昆虫分子鉴定中的应用[J].植物检疫,2010,24(2):46-50.
[27]杨倩倩,李志红,伍祎,等.线粒体COⅠ基因在昆虫DNA条形码中的研究与应用[J].应用昆虫学报,2012,49(6):1687-1695.
[28]Hua YQ,Ding YR,Yan ZT,et al.The complete mitochondrial genome of Anopheles minimus(Diptera∶Culicidae)and the phylogenetics of known Anopheles mitogenomes[J].Insect Sci,2016,23(3):353-365.
[29]Zhang H,Xing D,Wang G,et al.Sequencing and analysis of the complete mitochondrial genome of Aedes albopictus(Diptera∶Culicidae)in China[J].Mitochondrial DNA A DNA Mapp Seq Anal,2016,27(4):2787-2788.
[30]Sharma A,Deshmukh A,Sharma R,et al.Population genetic structure of malaria vector Anopheles stephensi using mitochondrial cytochrome oxidase II gene in Indian populations[J].Indian J Exp Biol,2014,52(10):996-1002.
[31]Khadem M.Deep interisland genetic divergence in the macaronesian endemic mosquito Ochlerotatus eatoni(Diptera∶Culicidae),Indication of cryptic species[J].J Med Entomol,2015,52(5):1175-1180.
[32]常雪莲,钟代斌,李小聪,等.基于mt DNA-COⅠ基因序列分析我国中华按蚊种群的遗传结构[J].南方医科大学学报,2015,35(2):234-238,247.
[33]阳帆,张仁利,刘阳,等.深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析[J].中国媒介生物学及控制杂志,2015,26(4):337-340.
[34]廉国胜,莫秋华,柯明剑,等.国境口岸13蚊种COⅠ部分基因序列分析及应用[J].热带医学杂志,2015,15(10):1321-1325.
[35]Murugan K,Vadivalagan C,Karthika P,et al.DNA barcoding and molecular evolution of mosquito vectors of medical and veterinary importance[J].Parasitol Res,2016,115(1):107-121.
[36]Ashfaq M,Hebert PD,Mirza JH,et al.Analyzing mosquito(Diptera∶Culicidae)diversity in Pakistan by DNA barcoding[J].PLo S One,2014,9(5):e97268.
[37]Chan A,Chiang LP,Hapuarachchi HC,et al.DNA barcoding:complementing morphological identification of mosquito species in Singapore[J].Parasit Vectors,2014,7:569.
[38]Oter K,Gunay F,Tuzer E,et al.First record of Stegomyia albopicta in Turkey determined by active ovitrap surveillance and DNA barcoding[J].Vector Borne Zoonotic Dis,2013,13(10):753-761.
[39]Engdahl C,Larsson P,N覿slund J,et al.Identification of Swedish mosquitoes based on molecular barcoding of the COⅠgene and SNP analysis[J].Mol Ecol Resour,2014,14(3):478-488.
[40]朱淮民.蚊虫采集、保存及标本制作[J].中国寄生虫学与寄生虫病杂志,2006,24(S1):73-75.
[41]Wijit A,Saeung A,Baimai V,et al.DNA barcoding for the identification of eight species members of the Thai Hyrcanus group and investigation of their stenogamous behavior[J].C R Biol,2013,336(9):449-456.
[42]Laboudi M,Faraj C,Sadak A,et al.DNA barcodes confirm the presence of a single member of the Anopheles maculipennis group in Morocco and Algeria:An.sicaulti is conspecific with An.labranchiae[J].Acta Trop,2011,118(1):6-13.
[43]Tahir HM,Kanwal N,Mehwish.The sequence divergence in cytochrome C oxidase I gene of Culex quinquefasciatus mosquito and its comparison with four other Culex species[J].Mitochondrial DNA A DNA Mapp Seq Anal,2016,27(4):3054-3057.
[44]Laurito M,Oliveira TM,Almirón WR,et al.COⅠbarcode versus morphological identification of Culex(Culex)(Diptera∶Culicidae)species:a case study using samples from Argentina and Brazil[J].Mem Inst Oswaldo Cruz,2013,108(Suppl 1):110-122.
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