等离子体聚合法制备生物大分子印迹聚合物
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摘要
分别以缩水甘油和二乙二醇单乙烯基醚(EO2)为单体,采用等离子体聚合方法,在金基底表面制备特异性识别牛血清蛋白(BSA)的生物大分子印迹聚合物(BMIPs).利用傅里叶变换红外光谱仪(FTIR)和接触角测试仪对沉积薄膜的物理化学特性进行表征,利用表面等离子体共振光谱(SPR)研究所制备印迹聚合物中BSA模板分子的洗脱和结合过程.实验结果表明,通过控制等离子体沉积过程中等离子体功率和占空比,可以制备对BSA具有一定特异性识别能力的BMIPs,这为将来以BMIPs为基础构建生物传感器提供了一种新的思路.
Biomacromolecularly imprinted polymers(BMIPs)on gold substrates were prepared with plasma polymerization method by using glycidol and di(ethylene glycol)monovinyl ether(EO2)as functional monomers,respectively.The physicochemical properties of the plasma deposited films were characterized by Fourier transform infrared spectroscopy(FTIR)and contact angle measurement.Surface plasmon resonance spectroscopy(SPR)was employed to monitor the desorption and adsorption of BSA template on the resulting BMIPs.Experimental results showed that,by adjusting the conditions of plasma input power and duty cycle during the deposition process,the resulting BMIPs can recognize BSA to some extent.The fabrication process can provide a new way for the construction of BMIPs-based biosensors.
Biomacromolecularly imprinted polymers(BMIPs)on gold substrates were prepared with plasma polymerization method by using glycidol and di(ethylene glycol)monovinyl ether(EO2)as functional monomers,respectively.The physicochemical properties of the plasma deposited films were characterized by Fourier transform infrared spectroscopy(FTIR)and contact angle measurement.Surface plasmon resonance spectroscopy(SPR)was employed to monitor the desorption and adsorption of BSA template on the resulting BMIPs.Experimental results showed that,by adjusting the conditions of plasma input power and duty cycle during the deposition process,the resulting BMIPs can recognize BSA to some extent.The fabrication process can provide a new way for the construction of BMIPs-based biosensors.
引文
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[4]Algieri C,Drioli E,Guzzo L,et al.Bio-mimetic sensors based on molecularly imprinted membranes[J].Sensors,2014,14(8):13863–13912.
[5]Wackerlig J,Lieberzeit P A.Molecularly imprinted polymer nanoparticles in chemical sensing:Synthesis,characterisation and application[J].Sensors and Actuators B:Chemical,2015,207:144–157.
[6]Chen F,Zhao W,Zhang J,et al.Magnetic twodimensional molecularly imprinted materials for the recognition and separation of proteins[J].Physical Chemistry Chemical Physics,2016,18(2):718–725.
[7]Huval C C,Bailey M J,Braunlin W H,et al.Novel cholesterol lowering polymeric drugs obtained by molecular imprinting[J].Macromolecules,2001,34(6):1548–1550.
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